Rmu_ssc0000112.1_g000016

Leukocyte receptor cluster

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000112.1
OG0009843
Physical Location & Seq
Reverse (-)
81880 .. 92004
10125 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3159 bp
atgttgaatgacgaggatctgtctgccgccgacggtccgacccggaagtcattaactcataggagctcatggtggcctcgatgccaacagcttccggttatagccgctcccaccgccgaaacccttcttgattcggatcctgaataccgcggatatgtgggcggaaccgaggaggtattgaaaattgtggaggaggtgaatcgccatgttgttaatgggaaccaaggacagacgccgtcatattttccatcaacaactgggtcggaagcgtcggcatggccaactcatcgggtggagaatagctccacggaaaatgggattcattctcattcggcttatcaatataatcagcatacacagccacctgcaagtagctcgtccagtttgggaacagtgaatgcaccacaagattacagtacctatgcgtcatacccaaatcctgctgatccatatggttatggaagtgcgggataccaaggttactataacaactatcagcagcaacccaatcagtcctactcacaacccgtaggagcatatcaaaatacaggtgctccttatcagcctatttcctcatttcagaatacagggtcttatgctgggtctgcaagttattcaagcacttactacaatcctgctgattatcagactgctggaggctacgcaagtagcggttacactaatcagactaatcagaccactgcttggaatggtggaaattatgcaaactatacctctcaacaatatgcaccctacactccagaaactcctggtgtatatagttctgctactgcaacttcaaccccacaaaattatcagcagcattataagcaatgggcagattattacaatcaaacagaagtcagttgtgctcctgggactgagaacttatctgttactagtacacagaatgtaggatgtcccgttccaggtgtcaccactgggtatcaaacctcaaacagccagccaccaccttcatacactcctgcatggaggccagaatctagttcatctgaattaccatcggtgcagtctggtgctgtagttggtggttctcatgatgcttactggaatcatgtggctcctacttcgcaatctcaaattcaacatatttctcctattcaagcacaatttcaaaagcctttggatcaaaaaacttcttatgatagctttcaggaccaacaaaaaactgcaacttctcaagcatcaagcattcagtatcctgcttctcagcagctgccatatgtgtctcatagttaccaaacaccttcacaagctgttccatctatagatacacaaagagtaaacaaactgcagattccaacgaaccctagaattacttcaaatatgactttgggtttacgaaaaactgacaaggatataaatacaaccagtccagcaggaaaacctgcttacattagtgtttcactgccaaaaccggttgagaaggtgccatccagtgctagtgctgattctctgcttaagactggtatgttccccaagtcactgcgtggttatgttgaaagggctttggctcgctgtaaagatgatagccagatggctgcctgtcaggttgtcatgaaggagatcatcaccaaggcaacagctgatagcacactttacacacgagactgggatactgagcctcttttccccctcccaaatgaagatgctacaaataaagagttaaattcttcaaaccttgtttcatcattggcaaagtttaaaagaagtccaagtaggcgatccaagagtagatgggaacctttaccagaggagaagtcagagaagtccgttgaaaggccagcctctttaaatagcggtggtctaaactatgctgcttggggacatgtcaatgataaggagagaaagccattgttgtggagtgctgggagtaagaatgataatatgagtagtgcaaagtttccttcactggagcagaaaagtgcaagtaagataacccaaaagccgtttaagaggcagcgcctttctgatggggtaggtgctactgagaatggtgatgcatctagtgatagtgatgcagaacaaggtttagcagcctactacgcgggagcaattgcccttgcagattcaccagaggaaagaaagagacgtgaacatcgctctaagcgttttgaaaaagtgcaaggacatcgaggacaaaataatcacttcaaacctaaaaatgctggtgctgggagcttgtataatagaagagctaatgccttggtgctcagcaaaaattttgaagatggcgctagcagggctgttgaagacattgactgggattctcttactgtcaaggggacctgccaagaaattgagaaacgttatttgcgcctcacttctgcacctgatcctgccactgttaggccggaggatgttcttgaaaaagctttgcttatggtacaaaattctcagaagaattacctatataaatgcgatcagctgaagtccattcgccaggatttgactgtgcaacgaattcgaaatcagctaacagtaaaggtgtatgaaactcatgctcggttagcattggaagttggagacctgccagaatataatcagtgccagtcacagttgaagtcgctctatgctgatggtattgagggatgtcatatggagttttctgcgtacaacttgctttgtgttatcctgcactcaaataataacagagatctgttatcatcaatggcaagcttgtcagatgaaacaaaaaagcatgaagctgtcaaacatgcccttgcagttcgagctgctgtgacttcgggaaattatgtcatgttcttcaggctctacaagacagctcccaacttaagcacctgccttatggatctttatgttgaaaagatgcggtttaaggcagtgagctgcatgtgccgatcatatcggcccacaatacctgtttcatatgttgctcaaattctggggtttacacccacaaatgaggaaagtggggagaaggattcagagggattggaggaatgcattgaatggttgaaactacatggtgcatgccttgctgcagataacaatggggaaatgcagcttgatgcaaagtctacttcttctagtctttatatgccagagactgatgctgtgtcccatggagatgccaatctcgctgttaatgatttttttacacggacaccttcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAC3_GANP

Protein Analysis

1052

Amino Acids

115.91

Weight (kDa)

6.37

Isoelectric Point (pI)

48.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 780 - 982 1.8e-25 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 828
AarI CACCTGC 2 cut(s) 373, 2831
AasI GACNNNNNNGTC 1 cut(s) 46
Acc36I ACCTGC 5 cut(s) 373, 1417, 2318, 2559, 2831
AccB1I GGYRCC 1 cut(s) 1450
AccB7I CCANNNNNTGG 1 cut(s) 705
AccBSI CCGCTC 1 cut(s) 107
AccI GTMKAC 1 cut(s) 3062
AccII CGCG 2 cut(s) 150, 2069
AclI AACGTT 1 cut(s) 2329
AclWI GGATC 8 cut(s) 24, 131, 144, 440, 1155, 1740, 2351, 2841
AcoI YGGCCR 1 cut(s) 278
AcsI RAATTY 6 cut(s) 1101, 1690, 2242, 2413, 2484, 2922
AcuI CTGAAG 2 cut(s) 2471, 2773
AcyI GRCGYC 1 cut(s) 233
AdeI CACNNNGTG 1 cut(s) 1511
AfaI GTAC 4 cut(s) 418, 904, 2409, 2636
AfiI CCNNNNNNNGG 4 cut(s) 705, 929, 1800, 2370
AflII CTTAAG 2 cut(s) 1481, 2815
AflIII ACRYGT 1 cut(s) 1849
AgeI ACCGGT 1 cut(s) 1438
AhlI ACTAGT 1 cut(s) 899
AjiI CACGTC 1 cut(s) 2114
AjnI CCWGG 4 cut(s) 769, 874, 928, 2463
AloI GAACNNNNNNTCC 2 cut(s) 909, 941
Alw21I GWGCWC 4 cut(s) 68, 556, 874, 2235
Alw26I GTCTC 5 cut(s) 1254, 1623, 2104, 2541, 3083
AlwI GGATC 8 cut(s) 24, 131, 144, 440, 1155, 1740, 2351, 2841
AlwNI CAGNNNCTG 2 cut(s) 1237, 3092
AoxI GGCC 6 cut(s) 74, 278, 995, 1802, 2372, 2891
ApoI RAATTY 6 cut(s) 1101, 1690, 2242, 2413, 2484, 2922
AsiGI ACCGGT 1 cut(s) 1438
Asp700I GAANNNNTTC 2 cut(s) 123, 1339
AspLEI GCGC 3 cut(s) 1986, 2258, 2340
AspS9I GGNCC 4 cut(s) 35, 1177, 2307, 2892
AsuC2I CCSGG 1 cut(s) 43
AsuHPI GGTGA 5 cut(s) 208, 928, 1585, 2030, 2085
AsuII TTCGAA 1 cut(s) 2488
AsuNHI GCTAGC 1 cut(s) 2258
AvaII GGWCC 3 cut(s) 35, 1177, 2307
BaeI ACNNNNGTAYC 2 cut(s) 463, 496
BalI TGGCCA 1 cut(s) 280
BamHI GGATCC 1 cut(s) 136
BanI GGYRCC 1 cut(s) 1450
BanII GRGCYC 1 cut(s) 68
BauI CACGAG 1 cut(s) 1626
BbsI GAAGAC 1 cut(s) 2280
Bbv12I GWGCWC 4 cut(s) 68, 556, 874, 2235
BccI CCATC 9 cut(s) 256, 1030, 1291, 1462, 1552, 1752, 1988, 2246, 2594
BceAI ACGGC 2 cut(s) 220, 1954
BcgI CGANNNNNNTGC 6 cut(s) 357, 391, 2135, 2169, 2468, 2502
BciT130I CCWGG 4 cut(s) 771, 876, 930, 2465
BciVI GTATCC 3 cut(s) 464, 1230, 1630
BcnI CCSGG 1 cut(s) 43
BcoDI GTCTC 5 cut(s) 1254, 1623, 2104, 2541, 3083
BcuI ACTAGT 1 cut(s) 899
BfaI CTAG 7 cut(s) 900, 1005, 1332, 1464, 2028, 2259, 3072
BfmI CTRYAG 4 cut(s) 1041, 1287, 1313, 3024
BfoI RGCGCY 2 cut(s) 1987, 2259
BfrI CTTAAG 2 cut(s) 1481, 2815
BfuAI ACCTGC 5 cut(s) 373, 1417, 2318, 2559, 2831
BfuI GTATCC 3 cut(s) 464, 1230, 1630
BglII AGATCT 1 cut(s) 2677
BlpI GCTNAGC 1 cut(s) 2234
Bme1390I CCNGG 5 cut(s) 43, 771, 876, 930, 2465
Bme18I GGWCC 3 cut(s) 35, 1177, 2307
BmgBI CACGTC 1 cut(s) 2114
BmgT120I GGNCC 4 cut(s) 35, 1177, 2307, 2892
BmiI GGNNCC 7 cut(s) 138, 166, 221, 1083, 1452, 1764, 2308
BmrFI CCNGG 5 cut(s) 43, 771, 876, 930, 2465
BmrI ACTGGG 4 cut(s) 267, 951, 1642, 2293
BmtI GCTAGC 1 cut(s) 2262
BmuI ACTGGG 4 cut(s) 267, 951, 1642, 2293
BpiI GAAGAC 1 cut(s) 2280
BplI GAGNNNNNCTC 2 cut(s) 287, 319
BpmI CTGGAG 3 cut(s) 675, 744, 1955
Bpu1102I GCTNAGC 1 cut(s) 2234
Bpu14I TTCGAA 1 cut(s) 2488
BpuEI CTTGAG 1 cut(s) 1185
BpuMI CCSGG 1 cut(s) 43
BsaBI GATNNNNATC 2 cut(s) 135, 3117
BsaHI GRCGYC 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 2541
BsaJI CCNNGG 9 cut(s) 148, 168, 223, 306, 475, 875, 1596, 2226, 3106
BsaWI WCCGGW 2 cut(s) 94, 1438
BsaXI ACNNNNNCTCC 2 cut(s) 538, 568
Bsc4I CCNNNNNNNGG 4 cut(s) 705, 929, 1800, 2370
Bse118I RCCGGY 1 cut(s) 1438
Bse3DI GCAATG 1 cut(s) 839
Bse8I GATNNNNATC 2 cut(s) 135, 3117
BseBI CCWGG 4 cut(s) 771, 876, 930, 2465
BseDI CCNNGG 9 cut(s) 148, 168, 223, 306, 475, 875, 1596, 2226, 3106
BseGI GGATG 4 cut(s) 923, 1454, 2386, 2618
BseJI GATNNNNATC 2 cut(s) 135, 3117
BseLI CCNNNNNNNGG 4 cut(s) 705, 929, 1800, 2370
BseMI GCAATG 1 cut(s) 839
BseMII CTCAG 6 cut(s) 873, 1244, 1632, 2001, 2248, 2432
BseRI GAGGAG 3 cut(s) 185, 206, 1790
BseYI CCCAGC 3 cut(s) 599, 1889, 2195
BsgI GTGCAG 3 cut(s) 1049, 2334, 2642
Bsh1236I CGCG 2 cut(s) 150, 2069
BshFI GGCC 6 cut(s) 76, 280, 997, 1804, 2374, 2893
BshNI GGYRCC 1 cut(s) 1450
BshTI ACCGGT 1 cut(s) 1438
BsiHKAI GWGCWC 4 cut(s) 68, 556, 874, 2235
BsiSI CCGG 4 cut(s) 43, 95, 1439, 2375
BslFI GGGAC 5 cut(s) 892, 906, 1860, 2320, 3088
BslI CCNNNNNNNGG 4 cut(s) 705, 929, 1800, 2370
BsmAI GTCTC 5 cut(s) 1254, 1623, 2104, 2541, 3083
BsmBI CGTCTC 1 cut(s) 2104
BsmFI GGGAC 5 cut(s) 892, 906, 1860, 2320, 3088
BsmI GAATGC 3 cut(s) 403, 1212, 2990
BsnI GGCC 6 cut(s) 76, 280, 997, 1804, 2374, 2893
Bso31I GGTCTC 1 cut(s) 2541
Bsp119I TTCGAA 1 cut(s) 2488
Bsp1286I GDGCHC 4 cut(s) 68, 556, 874, 2235
Bsp1720I GCTNAGC 1 cut(s) 2234
Bsp19I CCATGG 1 cut(s) 3106
BspANI GGCC 6 cut(s) 76, 280, 997, 1804, 2374, 2893
BspCNI CTCAG 6 cut(s) 874, 1243, 1633, 2002, 2247, 2431
BspFNI CGCG 2 cut(s) 150, 2069
BspHI TCATGA 2 cut(s) 1057, 1578
BspLI GGNNCC 7 cut(s) 138, 166, 221, 1083, 1452, 1764, 2308
BspMAI CTGCAG 2 cut(s) 1317, 3028
BspMI ACCTGC 5 cut(s) 373, 1417, 2318, 2559, 2831
BspOI GCTAGC 1 cut(s) 2262
BspPI GGATC 8 cut(s) 24, 131, 144, 440, 1155, 1740, 2351, 2841
BspQI GCTCTTC 1 cut(s) 2209
BspT104I TTCGAA 1 cut(s) 2488
BspT107I GGYRCC 1 cut(s) 1450
BspTI CTTAAG 2 cut(s) 1481, 2815
BspTNI GGTCTC 1 cut(s) 2541
BsrBI CCGCTC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 839
BsrFI RCCGGY 1 cut(s) 1438
BssAI RCCGGY 1 cut(s) 1438
BssECI CCNNGG 9 cut(s) 148, 168, 223, 306, 475, 875, 1596, 2226, 3106
BssNI GRCGYC 1 cut(s) 233
BssSI CACGAG 1 cut(s) 1626
BssT1I CCWWGG 5 cut(s) 223, 475, 1596, 2226, 3106
Bst2BI CACGAG 1 cut(s) 1626
Bst2UI CCWGG 4 cut(s) 771, 876, 930, 2465
Bst4CI ACNGT 8 cut(s) 35, 394, 416, 2299, 2368, 2476, 2503, 2580
Bst6I CTCTTC 1 cut(s) 2209
BstACI GRCGYC 1 cut(s) 233
BstAFI CTTAAG 2 cut(s) 1481, 2815
BstAPI GCANNNNNTGC 1 cut(s) 3020
BstBI TTCGAA 1 cut(s) 2488
BstC8I GCNNGC 6 cut(s) 965, 1537, 1806, 2260, 2698, 3016
BstDEI CTNAG 7 cut(s) 882, 1230, 1641, 2010, 2127, 2234, 2418
BstDSI CCRYGG 3 cut(s) 148, 306, 3106
BstF5I GGATG 4 cut(s) 923, 1454, 2386, 2618
BstFNI CGCG 2 cut(s) 150, 2069
BstH2I RGCGCY 2 cut(s) 1987, 2259
BstHHI GCGC 3 cut(s) 1986, 2258, 2340
BstMAI GTCTC 5 cut(s) 1254, 1623, 2104, 2541, 3083
BstNI CCWGG 4 cut(s) 771, 876, 930, 2465
BstNSI RCATGY 4 cut(s) 1853, 2741, 2878, 3018
BstSCI CCNGG 5 cut(s) 41, 769, 874, 928, 2463
BstSFI CTRYAG 4 cut(s) 1041, 1287, 1313, 3024
BstUI CGCG 2 cut(s) 150, 2069
BstV2I GAAGAC 1 cut(s) 2280
BstX2I RGATCY 4 cut(s) 16, 136, 2677, 2833
BstXI CCANNNNNNTGG 1 cut(s) 1881
BstYI RGATCY 4 cut(s) 16, 136, 2677, 2833
BsuI GTATCC 3 cut(s) 464, 1230, 1630
BsuRI GGCC 6 cut(s) 76, 280, 997, 1804, 2374, 2893
BtgI CCRYGG 3 cut(s) 148, 306, 3106
BtgZI GCGATG 1 cut(s) 2105
BtrI CACGTC 1 cut(s) 2114
BtsCI GGATG 4 cut(s) 923, 1454, 2386, 2618
BtsI GCAGTG 4 cut(s) 699, 1427, 1505, 2871
BveI ACCTGC 5 cut(s) 373, 1417, 2318, 2559, 2831
Cac8I GCNNGC 6 cut(s) 965, 1537, 1806, 2260, 2698, 3016
CaiI CAGNNNCTG 2 cut(s) 1237, 3092
CciI TCATGA 2 cut(s) 1057, 1578
CfoI GCGC 3 cut(s) 1986, 2258, 2340
Cfr10I RCCGGY 1 cut(s) 1438
Cfr13I GGNCC 4 cut(s) 35, 1177, 2307, 2892
Cfr42I CCGCGG 1 cut(s) 151
CpoI CGGWCCG 1 cut(s) 35
CseI GACGC 3 cut(s) 241, 258, 414
Csp6I GTAC 4 cut(s) 417, 903, 2408, 2635
CspAI ACCGGT 1 cut(s) 1438
CspI CGGWCCG 1 cut(s) 35
CviQI GTAC 4 cut(s) 417, 903, 2408, 2635
DdeI CTNAG 7 cut(s) 882, 1230, 1641, 2010, 2127, 2234, 2418
DraI TTTAAA 2 cut(s) 1726, 1815
DraIII CACNNNGTG 1 cut(s) 1511
DrdI GACNNNNNNGTC 1 cut(s) 46
DseDI GACNNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 278
Eam1104I CTCTTC 1 cut(s) 2209
EarI CTCTTC 1 cut(s) 2209
EciI GGCGGA 1 cut(s) 177
Ecl136II GAGCTC 1 cut(s) 66
Eco130I CCWWGG 5 cut(s) 223, 475, 1596, 2226, 3106
Eco24I GRGCYC 1 cut(s) 68
Eco31I GGTCTC 1 cut(s) 2541
Eco47I GGWCC 3 cut(s) 35, 1177, 2307
Eco53kI GAGCTC 1 cut(s) 66
Eco57I CTGAAG 2 cut(s) 2471, 2773
EcoICRI GAGCTC 1 cut(s) 66
EcoO109I RGGNCCY 1 cut(s) 2307
EcoRI GAATTC 1 cut(s) 2484
EcoRII CCWGG 4 cut(s) 769, 874, 928, 2463
EcoT14I CCWWGG 5 cut(s) 223, 475, 1596, 2226, 3106
EcoT22I ATGCAT 2 cut(s) 2026, 2990
EcoT38I GRGCYC 1 cut(s) 68
ErhI CCWWGG 5 cut(s) 223, 475, 1596, 2226, 3106
Esp3I CGTCTC 1 cut(s) 2104
FalI AAGNNNNNCTT 2 cut(s) 2385, 2417
FaqI GGGAC 5 cut(s) 892, 906, 1860, 2320, 3088
FauI CCCGC 2 cut(s) 460, 2062
FauNDI CATATG 4 cut(s) 451, 1243, 2619, 2911
FblI GTMKAC 1 cut(s) 3062
FokI GGATG 4 cut(s) 930, 1441, 2393, 2625
FriOI GRGCYC 1 cut(s) 68
FspBI CTAG 7 cut(s) 900, 1005, 1332, 1464, 2028, 2259, 3072
GlaI GCGC 3 cut(s) 1985, 2257, 2339
GsaI CCCAGC 3 cut(s) 603, 1893, 2199
GsuI CTGGAG 3 cut(s) 675, 744, 1955
HaeII RGCGCY 2 cut(s) 1987, 2259
HaeIII GGCC 6 cut(s) 76, 280, 997, 1804, 2374, 2893
HapII CCGG 4 cut(s) 43, 95, 1439, 2375
HgaI GACGC 3 cut(s) 241, 258, 414
HhaI GCGC 3 cut(s) 1986, 2258, 2340
Hin1I GRCGYC 1 cut(s) 233
Hin6I GCGC 3 cut(s) 1984, 2256, 2338
HinP1I GCGC 3 cut(s) 1984, 2256, 2338
HindIII AAGCTT 2 cut(s) 2394, 2698
HpaII CCGG 4 cut(s) 43, 95, 1439, 2375
HphI GGTGA 5 cut(s) 208, 928, 1585, 2030, 2085
Hpy166II GTNNAC 6 cut(s) 905, 1306, 1361, 2117, 2934, 3063
Hpy188III TCNNGA 8 cut(s) 128, 140, 761, 1058, 1175, 1579, 2387, 2769
Hpy8I GTNNAC 6 cut(s) 905, 1306, 1361, 2117, 2934, 3063
Hpy99I CGWCG 2 cut(s) 35, 274
HpyAV CCTTC 7 cut(s) 134, 984, 1278, 1441, 1576, 1938, 2956
HpyCH4III ACNGT 8 cut(s) 35, 394, 416, 2299, 2368, 2476, 2503, 2580
HpyCH4IV ACGT 2 cut(s) 2113, 2329
HpyF3I CTNAG 7 cut(s) 882, 1230, 1641, 2010, 2127, 2234, 2418
HpySE526I ACGT 2 cut(s) 2113, 2329
Hsp92I GRCGYC 1 cut(s) 233
HspAI GCGC 3 cut(s) 1984, 2256, 2338
KspI CCGCGG 1 cut(s) 151
LguI GCTCTTC 1 cut(s) 2209
MaeI CTAG 7 cut(s) 900, 1005, 1332, 1464, 2028, 2259, 3072
MaeII ACGT 2 cut(s) 2113, 2329
MaeIII GTNAC 8 cut(s) 479, 674, 895, 934, 1256, 1503, 2574, 2761
MbiI CCGCTC 1 cut(s) 107
MboII GAAGA 8 cut(s) 1679, 1686, 2226, 2261, 2285, 2434, 2779, 3060
MfeI CAATTG 1 cut(s) 2076
MflI RGATCY 4 cut(s) 16, 136, 2677, 2833
MhlI GDGCHC 4 cut(s) 68, 556, 874, 2235
MlsI TGGCCA 1 cut(s) 280
MluNI TGGCCA 1 cut(s) 280
MmeI TCCRAC 4 cut(s) 62, 243, 1346, 2524
Mox20I TGGCCA 1 cut(s) 280
Mph1103I ATGCAT 2 cut(s) 2026, 2990
MroXI GAANNNNTTC 2 cut(s) 123, 1339
MscI TGGCCA 1 cut(s) 280
MslI CAYNNNNRTG 3 cut(s) 1854, 1879, 3111
Msp20I TGGCCA 1 cut(s) 280
MspA1I CMGCKG 4 cut(s) 150, 1237, 1607, 2449
MspCI CTTAAG 2 cut(s) 1481, 2815
MspI CCGG 4 cut(s) 43, 95, 1439, 2375
MspR9I CCNGG 5 cut(s) 43, 771, 876, 930, 2465
MunI CAATTG 1 cut(s) 2076
Mva1269I GAATGC 3 cut(s) 403, 1212, 2990
MvaI CCWGG 4 cut(s) 771, 876, 930, 2465
MvnI CGCG 2 cut(s) 150, 2069
NciI CCSGG 1 cut(s) 43
NcoI CCATGG 1 cut(s) 3106
NdeI CATATG 4 cut(s) 451, 1243, 2619, 2911
NheI GCTAGC 1 cut(s) 2258
NlaIV GGNNCC 7 cut(s) 138, 166, 221, 1083, 1452, 1764, 2308
NmuCI GTSAC 4 cut(s) 934, 1503, 2574, 2761
NsiI ATGCAT 2 cut(s) 2026, 2990
NspI RCATGY 4 cut(s) 1853, 2741, 2878, 3018
NspV TTCGAA 1 cut(s) 2488
PaeI GCATGC 1 cut(s) 3018
PagI TCATGA 2 cut(s) 1057, 1578
PaqCI CACCTGC 2 cut(s) 373, 2831
PciI ACATGT 1 cut(s) 1849
PciSI GCTCTTC 1 cut(s) 2209
PcsI WCGNNNNNNNCGW 1 cut(s) 2128
PctI GAATGC 3 cut(s) 403, 1212, 2990
PdmI GAANNNNTTC 2 cut(s) 123, 1339
PflFI GACNNNGTC 1 cut(s) 235
PflMI CCANNNNNTGG 1 cut(s) 705
PinAI ACCGGT 1 cut(s) 1438
PpuMI RGGWCCY 1 cut(s) 2307
PscI ACATGT 1 cut(s) 1849
PsiI TTATAA 1 cut(s) 828
Psp124BI GAGCTC 1 cut(s) 68
Psp1406I AACGTT 1 cut(s) 2329
Psp5II RGGWCCY 1 cut(s) 2307
Psp6I CCWGG 4 cut(s) 769, 874, 928, 2463
PspFI CCCAGC 3 cut(s) 599, 1889, 2195
PspGI CCWGG 4 cut(s) 769, 874, 928, 2463
PspN4I GGNNCC 7 cut(s) 138, 166, 221, 1083, 1452, 1764, 2308
PspPI GGNCC 4 cut(s) 35, 1177, 2307, 2892
PspPPI RGGWCCY 1 cut(s) 2307
PstI CTGCAG 2 cut(s) 1317, 3028
PstNI CAGNNNCTG 2 cut(s) 1237, 3092
PsuI RGATCY 4 cut(s) 16, 136, 2677, 2833
PsyI GACNNNGTC 1 cut(s) 235
PvuII CAGCTG 3 cut(s) 1237, 1607, 2449
RsaI GTAC 4 cut(s) 418, 904, 2409, 2636
RsaNI GTAC 4 cut(s) 417, 903, 2408, 2635
RseI CAYNNNNRTG 3 cut(s) 1854, 1879, 3111
Rsr2I CGGWCCG 1 cut(s) 35
RsrII CGGWCCG 1 cut(s) 35
SacI GAGCTC 1 cut(s) 68
SacII CCGCGG 1 cut(s) 151
SapI GCTCTTC 1 cut(s) 2209
Sau96I GGNCC 4 cut(s) 35, 1177, 2307, 2892
ScrFI CCNGG 5 cut(s) 43, 771, 876, 930, 2465
SduI GDGCHC 4 cut(s) 68, 556, 874, 2235
SfcI CTRYAG 4 cut(s) 1041, 1287, 1313, 3024
Sfr303I CCGCGG 1 cut(s) 151
SfuI TTCGAA 1 cut(s) 2488
SgrBI CCGCGG 1 cut(s) 151
SinI GGWCC 3 cut(s) 35, 1177, 2307
SmiMI CAYNNNNRTG 3 cut(s) 1854, 1879, 3111
SmlI CTYRAG 3 cut(s) 1200, 1481, 2815
SmoI CTYRAG 3 cut(s) 1200, 1481, 2815
SpeI ACTAGT 1 cut(s) 899
SphI GCATGC 1 cut(s) 3018
SspMI CTAG 7 cut(s) 900, 1005, 1332, 1464, 2028, 2259, 3072
SstI GAGCTC 1 cut(s) 68
StyD4I CCNGG 5 cut(s) 41, 769, 874, 928, 2463
StyI CCWWGG 5 cut(s) 223, 475, 1596, 2226, 3106
TaaI ACNGT 8 cut(s) 35, 394, 416, 2299, 2368, 2476, 2503, 2580
TaiI ACGT 2 cut(s) 2116, 2332
TaqI TCGA 4 cut(s) 79, 2155, 2488, 2752
TatI WGTACW 1 cut(s) 902
TauI GCSGC 2 cut(s) 29, 107
TseFI GTSAC 4 cut(s) 934, 1503, 2574, 2761
Tsp45I GTSAC 4 cut(s) 934, 1503, 2574, 2761
TspGWI ACGGA 2 cut(s) 323, 1783
Tth111I GACNNNGTC 1 cut(s) 235
Van91I CCANNNNNTGG 1 cut(s) 705
Vha464I CTTAAG 2 cut(s) 1481, 2815
VpaK11BI GGWCC 3 cut(s) 35, 1177, 2307
XapI RAATTY 6 cut(s) 1101, 1690, 2242, 2413, 2484, 2922
XceI RCATGY 4 cut(s) 1853, 2741, 2878, 3018
XcmI CCANNNNNNNNNTGG 1 cut(s) 212
XmiI GTMKAC 1 cut(s) 3062
XmnI GAANNNNTTC 2 cut(s) 123, 1339
XspI CTAG 7 cut(s) 900, 1005, 1332, 1464, 2028, 2259, 3072
Zsp2I ATGCAT 2 cut(s) 2026, 2990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.