Rmu_ssc0000135.1_g000037

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000135.1
OG0017712
Physical Location & Seq
Reverse (-)
189009 .. 189434
426 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 426 bp
atgccgaagaacaaaggaaagggaggcaagaaccacaagagggggaagaacgaagccgacgacgagaagcgcgagcttctcttgaaagaggacggtcaggaatacgcgcaggtcacacgcatgttaggcaacggccgattggaagccaagtgccaggacggcgtggcccgcctctgccacatctccggcaagctacacaagaaggtctggatcgccgccggcgatattctcctcgttgggctaagggactaccaggatggcaaggctgatgtcatccacaagtacatgcccgacgaggcccggttgctcaaggctaatgggaggttgaatgacagtttcaggcccaatgagagcggcgaccatgatgatgacaactctggcgatggttacctagagtttgaggatgctgatgttgatacaatttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

141

Amino Acids

15.76

Weight (kDa)

5.73

Isoelectric Point (pI)

35.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 32 - 94 1.5e-23 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017712)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g16270
rosa_chinensis RchiOBHm_Chr1g0359281
rosa_laevigata RLG00000027886
rosa_multiflora Rmu_sc0039464.1_g000001 Rmu_ssc0000135.1_g000036 Rmu_ssc0000135.1_g000037
rosa_roxburghii Rroxscaffold_4G00297160
rosa_rugosa Rorug01G0269100 Rorug01G0269200
rosa_samantha Rh1AG283100 Rh1CG267100
rosa_wichuraiana Rw1G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 100
AccBSI CCGCTC 1 cut(s) 354
AccII CGCG 2 cut(s) 72, 107
AciI CCGC 3 cut(s) 169, 216, 354
AclWI GGATC 1 cut(s) 218
AcoI YGGCCR 1 cut(s) 133
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 1 cut(s) 40
AgsI TTSAA 2 cut(s) 85, 328
AjnI CCWGG 2 cut(s) 153, 252
AluBI AGCT 2 cut(s) 76, 193
AluI AGCT 2 cut(s) 76, 193
AlwI GGATC 1 cut(s) 218
AoxI GGCC 4 cut(s) 133, 165, 297, 341
AspLEI GCGC 2 cut(s) 72, 109
AspS9I GGNCC 3 cut(s) 166, 298, 342
AsuC2I CCSGG 1 cut(s) 301
BccI CCATC 2 cut(s) 251, 377
BceAI ACGGC 2 cut(s) 148, 175
BciT130I CCWGG 2 cut(s) 155, 254
BcnI CCSGG 1 cut(s) 301
BfaI CTAG 1 cut(s) 392
BfuAI ACCTGC 1 cut(s) 100
BglI GCCNNNNNGGC 1 cut(s) 159
BisI GCNGC 2 cut(s) 216, 355
BlsI GCNGC 2 cut(s) 217, 356
Bme1390I CCNGG 3 cut(s) 155, 254, 301
BmgT120I GGNCC 3 cut(s) 166, 298, 342
BmrFI CCNGG 3 cut(s) 155, 254, 301
BmsI GCATC 1 cut(s) 394
Bpu10I CCTNAGC 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 301
Bsc4I CCNNNNNNNGG 1 cut(s) 40
Bse118I RCCGGY 1 cut(s) 218
BseBI CCWGG 2 cut(s) 155, 254
BseGI GGATG 3 cut(s) 262, 273, 409
BseLI CCNNNNNNNGG 1 cut(s) 40
BseRI GAGGAG 1 cut(s) 221
BseX3I CGGCCG 1 cut(s) 133
Bsh1236I CGCG 2 cut(s) 72, 107
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 4 cut(s) 135, 167, 299, 343
BsiEI CGRYCG 1 cut(s) 136
BsiSI CCGG 3 cut(s) 186, 219, 301
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 1 cut(s) 40
BsmFI GGGAC 1 cut(s) 260
BsnI GGCC 4 cut(s) 135, 167, 299, 343
Bsp143I GATC 1 cut(s) 210
BspACI CCGC 3 cut(s) 169, 216, 354
BspANI GGCC 4 cut(s) 135, 167, 299, 343
BspFNI CGCG 2 cut(s) 72, 107
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 1 cut(s) 218
BsrBI CCGCTC 1 cut(s) 354
BsrFI RCCGGY 1 cut(s) 218
BssAI RCCGGY 1 cut(s) 218
BssMI GATC 1 cut(s) 210
Bst2UI CCWGG 2 cut(s) 155, 254
Bst4CI ACNGT 2 cut(s) 95, 335
BstC8I GCNNGC 4 cut(s) 74, 169, 191, 220
BstDEI CTNAG 1 cut(s) 242
BstEII GGTNACC 1 cut(s) 386
BstF5I GGATG 3 cut(s) 262, 273, 409
BstFNI CGCG 2 cut(s) 72, 107
BstHHI GCGC 2 cut(s) 72, 109
BstKTI GATC 1 cut(s) 213
BstMBI GATC 1 cut(s) 210
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 3 cut(s) 126, 159, 168
BstNI CCWGG 2 cut(s) 155, 254
BstNSI RCATGY 2 cut(s) 124, 289
BstPI GGTNACC 1 cut(s) 386
BstSCI CCNGG 3 cut(s) 153, 252, 299
BstUI CGCG 2 cut(s) 72, 107
BstZI CGGCCG 1 cut(s) 133
BsuRI GGCC 4 cut(s) 135, 167, 299, 343
BtgZI GCGATG 1 cut(s) 396
BtsCI GGATG 3 cut(s) 262, 273, 409
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 4 cut(s) 74, 169, 191, 220
CfoI GCGC 2 cut(s) 72, 109
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 3 cut(s) 166, 298, 342
Csp6I GTAC 1 cut(s) 283
CviAII CATG 3 cut(s) 121, 286, 362
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 1 cut(s) 242
DpnI GATC 1 cut(s) 212
DpnII GATC 1 cut(s) 210
EaeI YGGCCR 1 cut(s) 133
EagI CGGCCG 1 cut(s) 133
EclXI CGGCCG 1 cut(s) 133
Eco52I CGGCCG 1 cut(s) 133
Eco91I GGTNACC 1 cut(s) 386
EcoO65I GGTNACC 1 cut(s) 386
EcoRII CCWGG 2 cut(s) 153, 252
FaeI CATG 3 cut(s) 124, 289, 365
FaiI YATR 3 cut(s) 122, 287, 363
FaqI GGGAC 1 cut(s) 260
FatI CATG 3 cut(s) 120, 285, 361
FauI CCCGC 1 cut(s) 176
Fnu4HI GCNGC 2 cut(s) 216, 355
FokI GGATG 3 cut(s) 260, 269, 416
Fsp4HI GCNGC 2 cut(s) 216, 355
FspBI CTAG 1 cut(s) 392
GlaI GCGC 2 cut(s) 71, 108
GluI GCNGC 2 cut(s) 216, 355
HaeIII GGCC 4 cut(s) 135, 167, 299, 343
HapII CCGG 3 cut(s) 186, 219, 301
HhaI GCGC 2 cut(s) 72, 109
Hin1II CATG 3 cut(s) 124, 289, 365
Hin6I GCGC 2 cut(s) 70, 107
HinP1I GCGC 2 cut(s) 70, 107
HpaII CCGG 3 cut(s) 186, 219, 301
Hpy188III TCNNGA 3 cut(s) 82, 98, 208
Hpy99I CGWCG 3 cut(s) 62, 65, 296
HpyAV CCTTC 1 cut(s) 196
HpyCH4III ACNGT 2 cut(s) 95, 335
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 159, 168
HpyF3I CTNAG 1 cut(s) 242
Hsp92II CATG 3 cut(s) 124, 289, 365
HspAI GCGC 2 cut(s) 70, 107
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Kzo9I GATC 1 cut(s) 210
LweI GCATC 1 cut(s) 394
MaeI CTAG 1 cut(s) 392
MaeIII GTNAC 2 cut(s) 112, 386
MalI GATC 1 cut(s) 212
MbiI CCGCTC 1 cut(s) 354
MboI GATC 1 cut(s) 210
MboII GAAGA 2 cut(s) 19, 58
MluCI AATT 1 cut(s) 420
MnlI CCTC 8 cut(s) 17, 33, 82, 182, 242, 289, 315, 394
MreI CGCCGGCG 1 cut(s) 218
MroNI GCCGGC 1 cut(s) 218
MseI TTAA 1 cut(s) 424
MslI CAYNNNNRTG 2 cut(s) 119, 366
MspI CCGG 3 cut(s) 186, 219, 301
MspR9I CCNGG 3 cut(s) 155, 254, 301
MvaI CCWGG 2 cut(s) 155, 254
MvnI CGCG 2 cut(s) 72, 107
MwoI GCNNNNNNNGC 3 cut(s) 126, 159, 168
NaeI GCCGGC 1 cut(s) 220
NciI CCSGG 1 cut(s) 301
NdeII GATC 1 cut(s) 210
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 3 cut(s) 124, 289, 365
NmuCI GTSAC 1 cut(s) 112
NspI RCATGY 2 cut(s) 124, 289
PcsI WCGNNNNNNNCGW 3 cut(s) 57, 69, 219
PdiI GCCGGC 1 cut(s) 220
PkrI GCNGC 2 cut(s) 217, 356
Psp6I CCWGG 2 cut(s) 153, 252
PspEI GGTNACC 1 cut(s) 386
PspGI CCWGG 2 cut(s) 153, 252
PspPI GGNCC 3 cut(s) 166, 298, 342
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 2 cut(s) 119, 366
SaqAI TTAA 1 cut(s) 424
SatI GCNGC 2 cut(s) 216, 355
Sau3AI GATC 1 cut(s) 210
Sau96I GGNCC 3 cut(s) 166, 298, 342
ScrFI CCNGG 3 cut(s) 155, 254, 301
SetI ASST 6 cut(s) 78, 114, 195, 207, 326, 393
SfaNI GCATC 1 cut(s) 394
SgrAI CRCCGGYG 1 cut(s) 218
SmiMI CAYNNNNRTG 2 cut(s) 119, 366
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 1 cut(s) 420
SsiI CCGC 3 cut(s) 169, 216, 354
SspMI CTAG 1 cut(s) 392
StyD4I CCNGG 3 cut(s) 153, 252, 299
TaaI ACNGT 2 cut(s) 95, 335
TasI AATT 1 cut(s) 420
TatI WGTACW 1 cut(s) 282
TauI GCSGC 2 cut(s) 218, 357
Tru1I TTAA 1 cut(s) 424
Tru9I TTAA 1 cut(s) 424
TseFI GTSAC 1 cut(s) 112
Tsp45I GTSAC 1 cut(s) 112
XceI RCATGY 2 cut(s) 124, 289
XspI CTAG 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.