Rmu_ssc0000189.1_g000022

Seed dormancy control

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000189.1
OG0019361
Physical Location & Seq
Forward (+)
177461 .. 180249
2789 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 708 bp
atggaagatgaagcaacaagagattcgaattacaaagcaatgtggaatttggacttcttgttgagtaaggattggaattgccgtgagattcttgaaggttctagggagttcttgacgtttctacttcagaataggcgtggaagacatgtgagaggcatgtccttccgactcaattctgtcaatttcagccgtttagctgatgatgtggcgttccaagtcagcacaggtggggcccacacctacccctacctcttcaccaacctcctccgctccttcctcgactccgcctccgacgaagattccgacgccgactccgactccgacagcctcgtcgtccagttcaacgaccacccctggcgcagcccttcctcttctctcacacaaaaggtcgagcaggtggagtgcggtctcaggctcatggtccctgcgctggcggcgcgtgtgagggacgcccaggcagcgttcgtggagcgcgtggcgaggaactgggggccttacgacgccgggaagaaggaggcggcgaaggtggtgatggcggaggcgatggaggcggagatggtgagcgttctggtggatgcgaataggctcagtgcaagactcctctcagtaagcaatgccgcttggctatttgattctgggtctggtccaagaacaacacggcgatggtggtcaaatttctcaatggcatggccgagaatttggctctaa

Protein Analysis

235

Amino Acids

26.7

Weight (kDa)

5.7

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019361)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0125401
rosa_multiflora Rmu_sc0023345.1_g000001 Rmu_ssc0000189.1_g000022
rosa_roxburghii Rroxscaffold_1G00030940 Rroxscaffold_1G00030950 Rroxscaffold_2G00117580
rosa_samantha Rh6AG127100 Rh6BG229300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 385
AasI GACNNNNNNGTC 1 cut(s) 329
Acc36I ACCTGC 1 cut(s) 385
AccBSI CCGCTC 1 cut(s) 270
AccII CGCG 2 cut(s) 439, 474
AciI CCGC 8 cut(s) 268, 285, 405, 434, 518, 536, 551, 618
AcoI YGGCCR 1 cut(s) 689
AcsI RAATTY 3 cut(s) 46, 673, 696
AcuI CTGAAG 1 cut(s) 110
AcyI GRCGYC 3 cut(s) 306, 450, 501
AfiI CCNNNNNNNGG 1 cut(s) 430
AflIII ACRYGT 1 cut(s) 145
AgsI TTSAA 2 cut(s) 95, 343
AjnI CCWGG 2 cut(s) 353, 453
AluBI AGCT 1 cut(s) 197
AluI AGCT 1 cut(s) 197
Alw26I GTCTC 1 cut(s) 413
AoxI GGCC 3 cut(s) 231, 491, 689
ApaI GGGCCC 1 cut(s) 235
ApeKI GCWGC 2 cut(s) 360, 458
ApoI RAATTY 3 cut(s) 46, 673, 696
AspLEI GCGC 4 cut(s) 360, 430, 439, 474
AspS9I GGNCC 5 cut(s) 231, 232, 421, 491, 644
AsuC2I CCSGG 1 cut(s) 505
AsuHPI GGTGA 3 cut(s) 247, 541, 571
AsuII TTCGAA 1 cut(s) 26
AvaII GGWCC 2 cut(s) 421, 644
BaeGI GKGCMC 1 cut(s) 235
BanII GRGCYC 1 cut(s) 235
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 2 cut(s) 372, 470
BccI CCATC 4 cut(s) 526, 538, 550, 657
BceAI ACGGC 3 cut(s) 66, 174, 674
BciT130I CCWGG 2 cut(s) 355, 455
BcnI CCSGG 1 cut(s) 505
BcoDI GTCTC 1 cut(s) 413
BfaI CTAG 1 cut(s) 102
BfuAI ACCTGC 1 cut(s) 385
BisI GCNGC 5 cut(s) 361, 435, 459, 519, 618
BlsI GCNGC 5 cut(s) 362, 436, 460, 520, 619
Bme1390I CCNGG 3 cut(s) 355, 455, 505
Bme18I GGWCC 2 cut(s) 421, 644
BmgT120I GGNCC 5 cut(s) 231, 232, 421, 491, 644
BmiI GGNNCC 4 cut(s) 232, 233, 423, 492
BmrFI CCNGG 3 cut(s) 355, 455, 505
BmrI ACTGGG 1 cut(s) 496
BmsI GCATC 1 cut(s) 565
BmuI ACTGGG 1 cut(s) 496
BpiI GAAGAC 1 cut(s) 148
Bpu14I TTCGAA 1 cut(s) 26
BpuMI CCSGG 1 cut(s) 505
BsaHI GRCGYC 3 cut(s) 306, 450, 501
BsaI GGTCTC 1 cut(s) 413
BsaJI CCNNGG 2 cut(s) 353, 453
BsaXI ACNNNNNCTCC 6 cut(s) 272, 296, 302, 302, 326, 332
Bsc4I CCNNNNNNNGG 1 cut(s) 430
Bse1I ACTGG 2 cut(s) 337, 491
Bse3DI GCAATG 2 cut(s) 45, 619
BseBI CCWGG 2 cut(s) 355, 455
BseDI CCNNGG 2 cut(s) 353, 453
BseGI GGATG 1 cut(s) 580
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMI GCAATG 2 cut(s) 45, 619
BseMII CTCAG 3 cut(s) 424, 601, 618
BseNI ACTGG 2 cut(s) 337, 491
BseRI GAGGAG 2 cut(s) 254, 590
BseSI GKGCMC 1 cut(s) 235
BseXI GCAGC 2 cut(s) 372, 470
Bsh1236I CGCG 2 cut(s) 439, 474
BshFI GGCC 3 cut(s) 233, 493, 691
BsiSI CCGG 1 cut(s) 504
BslFI GGGAC 2 cut(s) 407, 461
BslI CCNNNNNNNGG 1 cut(s) 430
BsmAI GTCTC 1 cut(s) 413
BsmFI GGGAC 2 cut(s) 407, 461
BsnI GGCC 3 cut(s) 233, 493, 691
Bso31I GGTCTC 1 cut(s) 413
Bsp119I TTCGAA 1 cut(s) 26
Bsp120I GGGCCC 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 235
BspACI CCGC 8 cut(s) 268, 285, 405, 434, 518, 536, 551, 618
BspANI GGCC 3 cut(s) 233, 493, 691
BspCNI CTCAG 3 cut(s) 423, 600, 617
BspFNI CGCG 2 cut(s) 439, 474
BspLI GGNNCC 4 cut(s) 232, 233, 423, 492
BspMI ACCTGC 1 cut(s) 385
BspT104I TTCGAA 1 cut(s) 26
BspTNI GGTCTC 1 cut(s) 413
BsrBI CCGCTC 1 cut(s) 270
BsrDI GCAATG 2 cut(s) 45, 619
BsrI ACTGG 2 cut(s) 337, 491
BssECI CCNNGG 2 cut(s) 353, 453
BssNI GRCGYC 3 cut(s) 306, 450, 501
Bst2UI CCWGG 2 cut(s) 355, 455
Bst6I CTCTTC 2 cut(s) 257, 376
BstACI GRCGYC 3 cut(s) 306, 450, 501
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 3 cut(s) 410, 587, 604
BstF5I GGATG 1 cut(s) 580
BstFNI CGCG 2 cut(s) 439, 474
BstHHI GCGC 4 cut(s) 360, 430, 439, 474
BstMAI GTCTC 1 cut(s) 413
BstMWI GCNNNNNNNGC 4 cut(s) 434, 436, 458, 548
BstNI CCWGG 2 cut(s) 355, 455
BstNSI RCATGY 2 cut(s) 149, 160
BstSCI CCNGG 3 cut(s) 353, 453, 503
BstSLI GKGCMC 1 cut(s) 235
BstUI CGCG 2 cut(s) 439, 474
BstV1I GCAGC 2 cut(s) 372, 470
BstV2I GAAGAC 1 cut(s) 148
BsuRI GGCC 3 cut(s) 233, 493, 691
BtgZI GCGATG 2 cut(s) 557, 676
BtsCI GGATG 1 cut(s) 580
BtsIMutI CAGTG 1 cut(s) 595
BveI ACCTGC 1 cut(s) 385
Cac8I GCNNGC 1 cut(s) 432
CfoI GCGC 4 cut(s) 360, 430, 439, 474
Cfr13I GGNCC 5 cut(s) 231, 232, 421, 491, 644
CseI GACGC 3 cut(s) 314, 458, 509
CviAII CATG 4 cut(s) 146, 157, 418, 687
DdeI CTNAG 3 cut(s) 410, 587, 604
DrdI GACNNNNNNGTC 1 cut(s) 329
DseDI GACNNNNNNGTC 1 cut(s) 329
EaeI YGGCCR 1 cut(s) 689
Eam1104I CTCTTC 2 cut(s) 257, 376
EarI CTCTTC 2 cut(s) 257, 376
EciI GGCGGA 3 cut(s) 274, 551, 566
Eco24I GRGCYC 1 cut(s) 235
Eco31I GGTCTC 1 cut(s) 413
Eco47I GGWCC 2 cut(s) 421, 644
Eco57I CTGAAG 1 cut(s) 110
EcoO109I RGGNCCY 2 cut(s) 231, 491
EcoRII CCWGG 2 cut(s) 353, 453
EcoT38I GRGCYC 1 cut(s) 235
FaeI CATG 4 cut(s) 149, 160, 421, 690
FaiI YATR 4 cut(s) 147, 158, 419, 688
FaqI GGGAC 2 cut(s) 407, 461
FatI CATG 4 cut(s) 145, 156, 417, 686
Fnu4HI GCNGC 5 cut(s) 361, 435, 459, 519, 618
FokI GGATG 1 cut(s) 587
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 5 cut(s) 361, 435, 459, 519, 618
FspBI CTAG 1 cut(s) 102
GlaI GCGC 4 cut(s) 359, 429, 438, 473
GluI GCNGC 5 cut(s) 361, 435, 459, 519, 618
HaeIII GGCC 3 cut(s) 233, 493, 691
HapII CCGG 1 cut(s) 504
HgaI GACGC 3 cut(s) 314, 458, 509
HhaI GCGC 4 cut(s) 360, 430, 439, 474
Hin1I GRCGYC 3 cut(s) 306, 450, 501
Hin1II CATG 4 cut(s) 149, 160, 421, 690
Hin6I GCGC 4 cut(s) 358, 428, 437, 472
HinP1I GCGC 4 cut(s) 358, 428, 437, 472
HinfI GANTC 9 cut(s) 23, 88, 168, 281, 299, 311, 317, 597, 632
HpaII CCGG 1 cut(s) 504
HphI GGTGA 3 cut(s) 247, 541, 571
Hpy188I TCNGA 6 cut(s) 129, 167, 292, 304, 316, 322
Hpy188III TCNNGA 2 cut(s) 92, 112
Hpy99I CGWCG 4 cut(s) 296, 308, 335, 503
HpyAV CCTTC 6 cut(s) 89, 172, 283, 375, 505, 517
HpyCH4IV ACGT 1 cut(s) 116
HpyCH4V TGCA 1 cut(s) 593
HpyF10VI GCNNNNNNNGC 4 cut(s) 434, 436, 458, 548
HpyF3I CTNAG 3 cut(s) 410, 587, 604
HpySE526I ACGT 1 cut(s) 116
Hsp92I GRCGYC 3 cut(s) 306, 450, 501
Hsp92II CATG 4 cut(s) 149, 160, 421, 690
HspAI GCGC 4 cut(s) 358, 428, 437, 472
LmnI GCTCC 2 cut(s) 275, 469
Lsp1109I GCAGC 2 cut(s) 372, 470
LweI GCATC 1 cut(s) 565
MaeI CTAG 1 cut(s) 102
MaeII ACGT 1 cut(s) 116
MbiI CCGCTC 1 cut(s) 270
MboII GAAGA 6 cut(s) 17, 153, 244, 308, 363, 520
MhlI GDGCHC 1 cut(s) 235
MluCI AATT 7 cut(s) 28, 46, 76, 172, 181, 673, 696
MlyI GAGTC 5 cut(s) 162, 275, 305, 311, 591
MmeI TCCRAC 5 cut(s) 190, 315, 327, 339, 345
MslI CAYNNNNRTG 1 cut(s) 661
MspI CCGG 1 cut(s) 504
MspR9I CCNGG 3 cut(s) 355, 455, 505
MvaI CCWGG 2 cut(s) 355, 455
MvnI CGCG 2 cut(s) 439, 474
MwoI GCNNNNNNNGC 4 cut(s) 434, 436, 458, 548
NciI CCSGG 1 cut(s) 505
NlaIII CATG 4 cut(s) 149, 160, 421, 690
NlaIV GGNNCC 4 cut(s) 232, 233, 423, 492
NspI RCATGY 2 cut(s) 149, 160
NspV TTCGAA 1 cut(s) 26
PaqCI CACCTGC 1 cut(s) 385
PciI ACATGT 1 cut(s) 145
PcsI WCGNNNNNNNCGW 3 cut(s) 300, 312, 471
PfeI GAWTC 4 cut(s) 23, 88, 299, 632
PkrI GCNGC 5 cut(s) 362, 436, 460, 520, 619
PleI GAGTC 5 cut(s) 162, 275, 305, 311, 591
PpsI GAGTC 5 cut(s) 162, 275, 305, 311, 591
PscI ACATGT 1 cut(s) 145
Psp6I CCWGG 2 cut(s) 353, 453
PspGI CCWGG 2 cut(s) 353, 453
PspN4I GGNNCC 4 cut(s) 232, 233, 423, 492
PspOMI GGGCCC 1 cut(s) 231
PspPI GGNCC 5 cut(s) 231, 232, 421, 491, 644
RseI CAYNNNNRTG 1 cut(s) 661
SatI GCNGC 5 cut(s) 361, 435, 459, 519, 618
Sau96I GGNCC 5 cut(s) 231, 232, 421, 491, 644
SchI GAGTC 5 cut(s) 162, 275, 305, 311, 591
ScrFI CCNGG 3 cut(s) 355, 455, 505
SduI GDGCHC 1 cut(s) 235
SfaNI GCATC 1 cut(s) 565
SfuI TTCGAA 1 cut(s) 26
SinI GGWCC 2 cut(s) 421, 644
SmiMI CAYNNNNRTG 1 cut(s) 661
Sse9I AATT 7 cut(s) 28, 46, 76, 172, 181, 673, 696
SsiI CCGC 8 cut(s) 268, 285, 405, 434, 518, 536, 551, 618
SspMI CTAG 1 cut(s) 102
StyD4I CCNGG 3 cut(s) 353, 453, 503
TaiI ACGT 1 cut(s) 119
TaqI TCGA 3 cut(s) 26, 279, 390
TasI AATT 7 cut(s) 28, 46, 76, 172, 181, 673, 696
TauI GCSGC 3 cut(s) 437, 521, 620
TfiI GAWTC 4 cut(s) 23, 88, 299, 632
TscAI CASTG 1 cut(s) 595
TseI GCWGC 2 cut(s) 360, 458
TspDTI ATGAA 1 cut(s) 24
TspRI CASTG 1 cut(s) 595
VpaK11BI GGWCC 2 cut(s) 421, 644
XapI RAATTY 3 cut(s) 46, 673, 696
XceI RCATGY 2 cut(s) 149, 160
XspI CTAG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.