Rmu_ssc0000326.1_g000002

Protein MON2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000326.1
OG0007304
Physical Location & Seq
Reverse (-)
5881 .. 27417
21537 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4596 bp
atggctttcatggcggttttggagtcagatcttcgcgctctctccgccgaggctcgccgtcgctaccctgctgtcaaagacggcgctgagcacgcaattataaagctccgttctttgtcaagccctggtgagattgcgcagaatgaggacatattgagaatcttcctgatggcttgtgaggttaaaactgtgaagttgagtgttattggactctcgtgtctacagaagcttatatctcatgatgctgttgcttcatcagctctgcacgagatactttgtacattgaaggatcatgctgaaatgaccgatgagagtgttcagcttaagaccctgcagactgtattgataatattgcagtcacctttacaccctcagaatgaggacgatatggctcaagctcttggtatatgccttcggcttctcgaaaataggtcccctgacagtgtgcgcaatactgctgcagctactttcaggcaagctgtagcactgatttttgatcatgtggtttgcgctgagactcttcctgccggcaagcttagttctggaggttacatctctcggaccagtccagttagtggtgatgttagttgcagcataaatctttcagagtctttggatggatctgtttctggacagtcatcattgatgagggagacactaactaaagctggaaagcttgggcttcgtctgcttgaagacctgacagctcttgctgcaggtggatctgcaatctggttacgtgtcagttctctacagcggtcctttgcactcgacatactggagaatcccaagaacaccaacgttgtggagggcatggtgaaagctcttgctagagtagtttcaagtgtacaggttcaagagactagtgaagagagcctcgcagctgttgcaggaatgtttaacagcaaagctaaaggagttgagtggagccttgataatgatgcatccaatgctgcagtattggttgctagtgaagctcattgtataaccttggcagtcgaaggtctgctcggtgtagtcttcacagtagctacattgacggatgaagcagtagattccggagagattgaatcccccagatgtgactatgatccacctgcaaagaagactggaaatgctgctcttctctgtctctcaatggttgattcattatggttgacgatactagatgcattctcctttattttgtcaaggtcacaaggtgaggctattgtgttggaaatactgaagggatatcaggccttcacccaggcatgcggagttcttggtgctgtagaacctttgaactcctttttagcatcactttgcaaatttacaatcattttccccgttgaagcagaaaaaaggagcattactttacaatctcctgggtccaaacggtctgaacaagtagttgatcaaagggaaagtgttgttctcacccctaagaatgtccaggccttaagaactctcttcaacattgctcatagattacacaatgtgttgggcccatcctgggttttggtattagaaaccctagcagccctagaccgcgcaatccattctccgcatgctaccacacagctgtttgagagctcagcactgatgcacatatctgcagtaaaatcccttctttgtgcactgggccaactgtcacagcaatgcatggctgggatttcaaatggatctgtacctgcttcaagtcaaaaagttggaaacataaacttttcagtggagagaatgatatccatcctagtcaataatctacacagagtggagccattgtgggatcaagttgttggccactttcttgagcttgcagaaaattctaaccagcatttacgtaacatggcccttgatgcattagatgaatcaatatgtgctgttttaggttcagacaagttcccagacaacacaacaagcagattcaatggctcatctcagagtatggatactggggttacagatttgggatcacttgagtgtgctgtcatatctccactaagggttctttatctttctactcaaagtgttgactcacgtactggatctttgaaaattcttctgcatgttttagagaggcatggggagaaattacactacagttggccaaatattcttgaaatgttgaggtctgtagcggactcttcagataaggaactcattactctgggctttcagtgcctgcgggtgattatgaatgacggactttctactatacctgcagactgccttcaagtatgtgtagatgtgactggagcatacagtgctcaaaagacagagctgaatataagtctgactgcaataggcctcttatggacaacaactgactttattgtgaaagctctaatccatggacctggggcggagaagaaaacagggatttctgatgtgcatcctattctaaagccattggatggcgacataccaaaagaacaaacaatcaatggctcagataatgccaatgagcaagttccctcattaactatagttgacagtgacagactgcttttctctgttttctcattacttcaaaagcttggagccgatgagaggccagaggttagaaattcagcggtcaggacattgtttcagacacttggaagtcacgggcaaaaactttcgaaaagcatgtgggaagattgcctttggaattatgtgtttcctacactcgatcgtgcttctcacatggctgcaacttcatcgaaagatgaatggcatgggaaagaacttggaactcgtggaggaaaagcagttcatatgctcatacatcacagtcgcaacacagctcagaaacagtgggatgagacgttagtacttgtccttggtggcgtagcccgtattctacgctcattctttccttttcttagaagtttaagcaatttttggtcaggatgggagtctttgcttttatttgtgaagaatagtgtattaaatggaagcaaagaggttgcgcttgctgcaataaattgcttacagacacctattctttctcattcttccaaggggaacttgccgatgccttatcttgaatcagtacttgatgtttacgagcttgttcttcaaaattcaacaaacctgagtggaaatgcagccagcaaggtgaagcaggagattttgcagagtcttggggaactatatgtgcaagcacaaaggatgtttgatgatcggttgtatacacagttgcttgcagtcatacgcttggctatgaaacatgccatagttgccaacgataactgtgaaactgaatatggaaatgttccacctgtactacgtactgtgctcgagatcattccaatgttatgtccaacagagcacatttcttcaatgtggcttattcttcttcgggatttttctcaatttctgccaagattgaattctaccgtacaaattgaggaagatgaagcagaggaagctagcactagtgaccgagttccagatactcacttgaaaataaaacatgaaagacctaatgggacggcttcgatgactccaggagtaggagattctcctagttctagattaaagacatccgtaacagccagcattcctaattatatgtttgcggaaaagcttgttcccttgttggtagatcttttcctacgggctccagcagttgaaaagtatatcttgtatcctgaaattattcaaagtcttgggaggtgtatgactacaagaagagacaatccagatggttctctttggagattagctgtcgaaggcttcaatcgcattcttgttgatgatgccagcaactccactgtaaatgctggatcagattcaggtgctagtaagcctgcaagaacacgtatatggaaggaagttgccgatgtttatgaagtattccttgtgggttattgtggccgagctcttccttctgattccttctcaacagtggatgtaaaagctgacgagtcccttgagatgactatcttgcacattcttggcgacaagattttgaagtcaccaattgatgcacctaccgatattttgcagcgtctcgtttccactttggaccgttgtgcttcacgtactggttctttacctgttgattttgtggaacgtatgccttcccactgcagcaggttttccttgatttgcttacagaagttgttttccttgagcagttatgacaccaaagaccatgattggaattcagcgagagctgaggtgagtaaaattgcaatcatgatactgatgatgagatgtgaatacatcttaagcagatttctgaatgacgaaaatgatttagattgtacacctttcagccatccaactattcctgcaggtggacgcccattgccagcagcaagacttgaagaaatcatatatgttcttgaagagttgggccgactagtaattcattcagatactgcatccattcttcccttgcaaccatatttgaaaggtgctctatcaagagacaagaatcatgatgaccgttcgcaccttcttgttctatttccatccttttctgagctcatagtgtcaagagaagcaaaagtaagagcattggttcaagtcttgtttagactcatcagcaaagaattgtcacttgaaagagtttcccttaccagtcaagctgtggaagaatctcaaaggataccgcagtcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7_N

Protein Analysis

1531

Amino Acids

167.76

Weight (kDa)

5.63

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 3 - 169 1.1e-38 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HDS PF09324 657 - 723 7.7e-07 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 728 - 1014 5.4e-49 C-terminal region of Mon2 protein
Mon2_C PF16206 1178 - 1447 3.3e-10 C-terminal region of Mon2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 101
AarI CACCTGC 3 cut(s) 707, 1105, 4256
AasI GACNNNNNNGTC 1 cut(s) 216
Acc16I TGCGCA 2 cut(s) 138, 449
Acc36I ACCTGC 6 cut(s) 707, 1105, 1681, 2209, 4053, 4256
AccB7I CCANNNNNTGG 2 cut(s) 575, 2396
AccI GTMKAC 2 cut(s) 220, 3155
AccII CGCG 2 cut(s) 36, 1533
AccIII TCCGGA 1 cut(s) 1058
AclI AACGTT 1 cut(s) 801
AclWI GGATC 9 cut(s) 297, 628, 730, 1085, 1672, 1776, 1960, 2035, 3760
AcoI YGGCCR 3 cut(s) 1780, 2087, 3841
AcsI RAATTY 7 cut(s) 1310, 1804, 2037, 2548, 3046, 3355, 4132
AcuI CTGAAG 2 cut(s) 1247, 2112
AcyI GRCGYC 1 cut(s) 4273
AdeI CACNNNGTG 3 cut(s) 1202, 1480, 1753
AfiI CCNNNNNNNGG 6 cut(s) 575, 1495, 2396, 2532, 3479, 4268
AflII CTTAAG 3 cut(s) 323, 1441, 4198
AflIII ACRYGT 2 cut(s) 739, 3785
AhlI ACTAGT 3 cut(s) 863, 3401, 4333
AjnI CCWGG 7 cut(s) 124, 1248, 1366, 1434, 1493, 2338, 3472
AjuI GAANNNNNNNTTGG 2 cut(s) 3340, 3372
AleI CACNNNNGTG 1 cut(s) 1960
AloI GAACNNNNNNTCC 2 cut(s) 2718, 2750
Alw21I GWGCWC 9 cut(s) 93, 1577, 1621, 2251, 3264, 3297, 3850, 4393, 4461
Alw26I GTCTC 8 cut(s) 509, 647, 854, 1136, 2780, 3654, 3983, 4395
Alw44I GTGCAC 1 cut(s) 1617
AlwI GGATC 9 cut(s) 297, 628, 730, 1085, 1672, 1776, 1960, 2035, 3760
AlwNI CAGNNNCTG 2 cut(s) 2164, 4352
Ama87I CYCGRG 1 cut(s) 3263
Aor13HI TCCGGA 1 cut(s) 1058
ApaI GGGCCC 1 cut(s) 1490
ApaLI GTGCAC 1 cut(s) 1617
ApoI RAATTY 7 cut(s) 1310, 1804, 2037, 2548, 3046, 3355, 4132
Asp700I GAANNNNTTC 1 cut(s) 3624
AspLEI GCGC 7 cut(s) 38, 86, 139, 450, 512, 1535, 2935
AsuII TTCGAA 1 cut(s) 2603
AsuNHI GCTAGC 1 cut(s) 3395
AvaI CYCGRG 1 cut(s) 3263
AvaII GGWCC 6 cut(s) 432, 561, 759, 1371, 2336, 3994
BaeGI GKGCMC 2 cut(s) 1490, 1621
BaeI ACNNNNGTAYC 3 cut(s) 1923, 1956, 4574
BalI TGGCCA 2 cut(s) 1782, 2089
BanII GRGCYC 5 cut(s) 1490, 1577, 3589, 3850, 4461
BarI GAAGNNNNNNTAC 2 cut(s) 1593, 1625
BauI CACGAG 3 cut(s) 214, 266, 2718
BbsI GAAGAC 3 cut(s) 702, 1012, 1112
Bbv12I GWGCWC 9 cut(s) 93, 1577, 1621, 2251, 3264, 3297, 3850, 4393, 4461
BbvCI CCTCAGC 1 cut(s) 4146
BccI CCATC 9 cut(s) 163, 611, 1498, 1736, 2390, 2868, 3665, 4257, 4453
BceAI ACGGC 3 cut(s) 42, 97, 3474
BcgI CGANNNNNNTGC 2 cut(s) 2664, 2698
BciT130I CCWGG 7 cut(s) 126, 1250, 1368, 1436, 1495, 2340, 3474
BciVI GTATCC 3 cut(s) 1924, 3624, 4575
BclI TGATCA 2 cut(s) 496, 1396
BcoDI GTCTC 8 cut(s) 509, 647, 854, 1136, 2780, 3654, 3983, 4395
BcuI ACTAGT 3 cut(s) 863, 3401, 4333
BfoI RGCGCY 1 cut(s) 87
BfrI CTTAAG 3 cut(s) 323, 1441, 4198
BfuAI ACCTGC 6 cut(s) 707, 1105, 1681, 2209, 4053, 4256
BfuI GTATCC 3 cut(s) 1924, 3624, 4575
BglII AGATCT 2 cut(s) 28, 3571
BlpI GCTNAGC 2 cut(s) 87, 1576
BmcAI AGTACT 2 cut(s) 2796, 3018
Bme1390I CCNGG 7 cut(s) 126, 1250, 1368, 1436, 1495, 2340, 3474
Bme18I GGWCC 6 cut(s) 432, 561, 759, 1371, 2336, 3994
BmeT110I CYCGRG 1 cut(s) 3263
BmiI GGNNCC 7 cut(s) 434, 929, 1372, 1488, 1758, 2524, 3588
BmrFI CCNGG 7 cut(s) 126, 1250, 1368, 1436, 1495, 2340, 3474
BmrI ACTGGG 2 cut(s) 1631, 1944
BmtI GCTAGC 1 cut(s) 3399
BmuI ACTGGG 2 cut(s) 1631, 1944
BpiI GAAGAC 3 cut(s) 702, 1012, 1112
BpmI CTGGAG 5 cut(s) 564, 800, 2256, 3456, 3573
Bpu10I CCTNAGC 1 cut(s) 4146
Bpu1102I GCTNAGC 2 cut(s) 87, 1576
Bpu14I TTCGAA 1 cut(s) 2603
BpuEI CTTGAG 5 cut(s) 378, 1811, 1979, 3920, 4120
BsaAI YACGTR 6 cut(s) 740, 1823, 2021, 3254, 3788, 4010
BsaBI GATNNNNATC 3 cut(s) 1727, 2373, 3908
BsaHI GRCGYC 1 cut(s) 4273
BsaWI WCCGGW 1 cut(s) 1058
BsaXI ACNNNNNCTCC 2 cut(s) 1748, 1778
Bsc4I CCNNNNNNNGG 6 cut(s) 575, 1495, 2396, 2532, 3479, 4268
Bse118I RCCGGY 1 cut(s) 527
Bse3DI GCAATG 3 cut(s) 1458, 1646, 4277
Bse8I GATNNNNATC 3 cut(s) 1727, 2373, 3908
BseAI TCCGGA 1 cut(s) 1058
BseBI CCWGG 7 cut(s) 126, 1250, 1368, 1436, 1495, 2340, 3474
BseJI GATNNNNATC 3 cut(s) 1727, 2373, 3908
BseLI CCNNNNNNNGG 6 cut(s) 575, 1495, 2396, 2532, 3479, 4268
BseMI GCAATG 3 cut(s) 1458, 1646, 4277
BseSI GKGCMC 2 cut(s) 1490, 1621
BseYI CCCAGC 1 cut(s) 1649
BsgI GTGCAG 1 cut(s) 248
Bsh1236I CGCG 2 cut(s) 36, 1533
Bsh1285I CGRYCG 1 cut(s) 2656
BsiEI CGRYCG 1 cut(s) 2656
BsiHKAI GWGCWC 9 cut(s) 93, 1577, 1621, 2251, 3264, 3297, 3850, 4393, 4461
BsiHKCI CYCGRG 1 cut(s) 3263
BsiSI CCGG 2 cut(s) 528, 1059
BslFI GGGAC 3 cut(s) 418, 3469, 3880
BslI CCNNNNNNNGG 6 cut(s) 575, 1495, 2396, 2532, 3479, 4268
BsmAI GTCTC 8 cut(s) 509, 647, 854, 1136, 2780, 3654, 3983, 4395
BsmBI CGTCTC 2 cut(s) 2780, 3983
BsmFI GGGAC 3 cut(s) 418, 3469, 3880
BsmI GAATGC 3 cut(s) 1172, 3525, 3711
BsoBI CYCGRG 1 cut(s) 3263
Bsp119I TTCGAA 1 cut(s) 2603
Bsp120I GGGCCC 1 cut(s) 1486
Bsp13I TCCGGA 1 cut(s) 1058
Bsp1407I TGTACA 3 cut(s) 278, 847, 4235
Bsp1720I GCTNAGC 2 cut(s) 87, 1576
Bsp19I CCATGG 1 cut(s) 2332
BspEI TCCGGA 1 cut(s) 1058
BspFNI CGCG 2 cut(s) 36, 1533
BspHI TCATGA 3 cut(s) 238, 4167, 4411
BspLI GGNNCC 7 cut(s) 434, 929, 1372, 1488, 1758, 2524, 3588
BspMAI CTGCAG 8 cut(s) 336, 463, 718, 958, 1600, 2206, 4061, 4267
BspMI ACCTGC 6 cut(s) 707, 1105, 1681, 2209, 4053, 4256
BspOI GCTAGC 1 cut(s) 3399
BspPI GGATC 9 cut(s) 297, 628, 730, 1085, 1672, 1776, 1960, 2035, 3760
BspQI GCTCTTC 2 cut(s) 1128, 3855
BspT104I TTCGAA 1 cut(s) 2603
BspTI CTTAAG 3 cut(s) 323, 1441, 4198
BsrDI GCAATG 3 cut(s) 1458, 1646, 4277
BsrFI RCCGGY 1 cut(s) 527
BsrGI TGTACA 3 cut(s) 278, 847, 4235
BssAI RCCGGY 1 cut(s) 527
BssNAI GTATAC 1 cut(s) 3156
BssNI GRCGYC 1 cut(s) 4273
BssSI CACGAG 3 cut(s) 214, 266, 2718
BssT1I CCWWGG 4 cut(s) 990, 2332, 2803, 2982
Bst1107I GTATAC 1 cut(s) 3156
Bst2BI CACGAG 3 cut(s) 214, 266, 2718
Bst2UI CCWGG 7 cut(s) 126, 1250, 1368, 1436, 1495, 2340, 3474
Bst6I CTCTTC 8 cut(s) 525, 864, 1128, 1457, 2131, 3652, 3855, 4314
BstACI GRCGYC 1 cut(s) 4273
BstAFI CTTAAG 3 cut(s) 323, 1441, 4198
BstAPI GCANNNNNTGC 3 cut(s) 887, 950, 2246
BstAUI TGTACA 3 cut(s) 278, 847, 4235
BstBAI YACGTR 6 cut(s) 740, 1823, 2021, 3254, 3788, 4010
BstBI TTCGAA 1 cut(s) 2603
BstDSI CCRYGG 1 cut(s) 2332
BstFNI CGCG 2 cut(s) 36, 1533
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 7 cut(s) 38, 86, 139, 450, 512, 1535, 2935
BstMAI GTCTC 8 cut(s) 509, 647, 854, 1136, 2780, 3654, 3983, 4395
BstMCI CGRYCG 1 cut(s) 2656
BstNI CCWGG 7 cut(s) 126, 1250, 1368, 1436, 1495, 2340, 3474
BstNSI RCATGY 5 cut(s) 1257, 1553, 2051, 2614, 3197
BstSCI CCNGG 7 cut(s) 124, 1248, 1366, 1434, 1493, 2338, 3472
BstSLI GKGCMC 2 cut(s) 1490, 1621
BstSNI TACGTA 2 cut(s) 1823, 3254
BstUI CGCG 2 cut(s) 36, 1533
BstV2I GAAGAC 3 cut(s) 702, 1012, 1112
BstX2I RGATCY 6 cut(s) 28, 620, 722, 1664, 2027, 3571
BstXI CCANNNNNNTGG 2 cut(s) 805, 2339
BstYI RGATCY 6 cut(s) 28, 620, 722, 1664, 2027, 3571
BstZ17I GTATAC 1 cut(s) 3156
BsuI GTATCC 3 cut(s) 1924, 3624, 4575
BtgI CCRYGG 1 cut(s) 2332
BtsI GCAGTG 1 cut(s) 4054
BveI ACCTGC 6 cut(s) 707, 1105, 1681, 2209, 4053, 4256
CaiI CAGNNNCTG 2 cut(s) 2164, 4352
CciI TCATGA 3 cut(s) 238, 4167, 4411
CfoI GCGC 7 cut(s) 38, 86, 139, 450, 512, 1535, 2935
Cfr10I RCCGGY 1 cut(s) 527
CseI GACGC 2 cut(s) 3965, 4281
DraIII CACNNNGTG 3 cut(s) 1202, 1480, 1753
DrdI GACNNNNNNGTC 1 cut(s) 216
DseDI GACNNNNNNGTC 1 cut(s) 216
EaeI YGGCCR 3 cut(s) 1780, 2087, 3841
Eam1104I CTCTTC 8 cut(s) 525, 864, 1128, 1457, 2131, 3652, 3855, 4314
EarI CTCTTC 8 cut(s) 525, 864, 1128, 1457, 2131, 3652, 3855, 4314
EciI GGCGGA 2 cut(s) 34, 2360
Ecl136II GAGCTC 3 cut(s) 1575, 3848, 4459
Eco105I TACGTA 2 cut(s) 1823, 3254
Eco130I CCWWGG 4 cut(s) 990, 2332, 2803, 2982
Eco147I AGGCCT 3 cut(s) 1241, 1439, 2289
Eco24I GRGCYC 5 cut(s) 1490, 1577, 3589, 3850, 4461
Eco32I GATATC 2 cut(s) 1235, 1725
Eco47I GGWCC 6 cut(s) 432, 561, 759, 1371, 2336, 3994
Eco53kI GAGCTC 3 cut(s) 1575, 3848, 4459
Eco57I CTGAAG 2 cut(s) 1247, 2112
Eco88I CYCGRG 1 cut(s) 3263
EcoICRI GAGCTC 3 cut(s) 1575, 3848, 4459
EcoO109I RGGNCCY 1 cut(s) 432
EcoRI GAATTC 2 cut(s) 3355, 4132
EcoRII CCWGG 7 cut(s) 124, 1248, 1366, 1434, 1493, 2338, 3472
EcoRV GATATC 2 cut(s) 1235, 1725
EcoT14I CCWWGG 4 cut(s) 990, 2332, 2803, 2982
EcoT22I ATGCAT 4 cut(s) 946, 1174, 1646, 1843
EcoT38I GRGCYC 5 cut(s) 1490, 1577, 3589, 3850, 4461
ErhI CCWWGG 4 cut(s) 990, 2332, 2803, 2982
Esp3I CGTCTC 2 cut(s) 2780, 3983
FalI AAGNNNNNCTT 8 cut(s) 2610, 2642, 2975, 3007, 3593, 3625, 3810, 3842
FaqI GGGAC 3 cut(s) 418, 3469, 3880
FauI CCCGC 1 cut(s) 2160
FauNDI CATATG 1 cut(s) 2739
FbaI TGATCA 2 cut(s) 496, 1396
FblI GTMKAC 2 cut(s) 220, 3155
FriOI GRGCYC 5 cut(s) 1490, 1577, 3589, 3850, 4461
FspI TGCGCA 2 cut(s) 138, 449
GlaI GCGC 7 cut(s) 37, 85, 138, 449, 511, 1534, 2934
GsaI CCCAGC 1 cut(s) 1653
GsuI CTGGAG 5 cut(s) 564, 800, 2256, 3456, 3573
HaeII RGCGCY 1 cut(s) 87
HapII CCGG 2 cut(s) 528, 1059
HgaI GACGC 2 cut(s) 3965, 4281
HhaI GCGC 7 cut(s) 38, 86, 139, 450, 512, 1535, 2935
Hin1I GRCGYC 1 cut(s) 4273
Hin6I GCGC 7 cut(s) 36, 84, 137, 448, 510, 1533, 2933
HinP1I GCGC 7 cut(s) 36, 84, 137, 448, 510, 1533, 2933
HincII GTYRAC 3 cut(s) 1158, 2014, 2473
HindII GTYRAC 3 cut(s) 1158, 2014, 2473
HindIII AAGCTT 5 cut(s) 227, 533, 674, 2516, 3551
HpaII CCGG 2 cut(s) 528, 1059
Hpy99I CGWCG 1 cut(s) 63
HpyCH4IV ACGT 9 cut(s) 739, 801, 1822, 2020, 2789, 3253, 3787, 4009, 4042
HpySE526I ACGT 9 cut(s) 739, 801, 1822, 2020, 2789, 3253, 3787, 4009, 4042
Hsp92I GRCGYC 1 cut(s) 4273
HspAI GCGC 7 cut(s) 36, 84, 137, 448, 510, 1533, 2933
Kpn2I TCCGGA 1 cut(s) 1058
KroI GCCGGC 1 cut(s) 527
KroNI GCCGGC 1 cut(s) 529
Ksp22I TGATCA 2 cut(s) 496, 1396
LguI GCTCTTC 2 cut(s) 1128, 3855
LmnI GCTCC 7 cut(s) 111, 927, 1347, 1756, 2237, 2522, 3592
MaeII ACGT 9 cut(s) 739, 801, 1822, 2020, 2789, 3253, 3787, 4009, 4042
MfeI CAATTG 1 cut(s) 3948
MflI RGATCY 6 cut(s) 28, 620, 722, 1664, 2027, 3571
MlsI TGGCCA 2 cut(s) 1782, 2089
MluNI TGGCCA 2 cut(s) 1782, 2089
MmeI TCCRAC 4 cut(s) 1197, 1672, 3311, 4277
Mox20I TGGCCA 2 cut(s) 1782, 2089
Mph1103I ATGCAT 4 cut(s) 946, 1174, 1646, 1843
MroI TCCGGA 1 cut(s) 1058
MroNI GCCGGC 1 cut(s) 527
MroXI GAANNNNTTC 1 cut(s) 3624
MscI TGGCCA 2 cut(s) 1782, 2089
MseI TTAA 9 cut(s) 183, 324, 900, 1442, 2462, 2855, 2912, 3503, 4199
MslI CAYNNNNRTG 4 cut(s) 1639, 1960, 3275, 3790
Msp20I TGGCCA 2 cut(s) 1782, 2089
MspA1I CMGCKG 4 cut(s) 757, 884, 1564, 2555
MspCI CTTAAG 3 cut(s) 323, 1441, 4198
MspI CCGG 2 cut(s) 528, 1059
MspR9I CCNGG 7 cut(s) 126, 1250, 1368, 1436, 1495, 2340, 3474
MunI CAATTG 1 cut(s) 3948
Mva1269I GAATGC 3 cut(s) 1172, 3525, 3711
MvaI CCWGG 7 cut(s) 126, 1250, 1368, 1436, 1495, 2340, 3474
MvnI CGCG 2 cut(s) 36, 1533
NaeI GCCGGC 1 cut(s) 529
NcoI CCATGG 1 cut(s) 2332
NdeI CATATG 1 cut(s) 2739
NgoMIV GCCGGC 1 cut(s) 527
NheI GCTAGC 1 cut(s) 3395
NlaIV GGNNCC 7 cut(s) 434, 929, 1372, 1488, 1758, 2524, 3588
NmeAIII GCCGAG 2 cut(s) 73, 3869
NsbI TGCGCA 2 cut(s) 138, 449
NsiI ATGCAT 4 cut(s) 946, 1174, 1646, 1843
NspI RCATGY 5 cut(s) 1257, 1553, 2051, 2614, 3197
NspV TTCGAA 1 cut(s) 2603
OliI CACNNNNGTG 1 cut(s) 1960
PaeI GCATGC 2 cut(s) 1257, 1553
PaeR7I CTCGAG 1 cut(s) 3263
PagI TCATGA 3 cut(s) 238, 4167, 4411
PaqCI CACCTGC 3 cut(s) 707, 1105, 4256
PceI AGGCCT 3 cut(s) 1241, 1439, 2289
PciSI GCTCTTC 2 cut(s) 1128, 3855
PctI GAATGC 3 cut(s) 1172, 3525, 3711
PdiI GCCGGC 1 cut(s) 529
PdmI GAANNNNTTC 1 cut(s) 3624
PflMI CCANNNNNTGG 2 cut(s) 575, 2396
PfoI TCCNGGA 1 cut(s) 3472
Ple19I CGATCG 1 cut(s) 2656
Ppu21I YACGTR 6 cut(s) 740, 1823, 2021, 3254, 3788, 4010
PpuMI RGGWCCY 1 cut(s) 432
PsiI TTATAA 1 cut(s) 101
Psp124BI GAGCTC 3 cut(s) 1577, 3850, 4461
Psp1406I AACGTT 1 cut(s) 801
Psp5II RGGWCCY 1 cut(s) 432
Psp6I CCWGG 7 cut(s) 124, 1248, 1366, 1434, 1493, 2338, 3472
PspFI CCCAGC 1 cut(s) 1649
PspGI CCWGG 7 cut(s) 124, 1248, 1366, 1434, 1493, 2338, 3472
PspN4I GGNNCC 7 cut(s) 434, 929, 1372, 1488, 1758, 2524, 3588
PspOMI GGGCCC 1 cut(s) 1486
PspPPI RGGWCCY 1 cut(s) 432
PstI CTGCAG 8 cut(s) 336, 463, 718, 958, 1600, 2206, 4061, 4267
PstNI CAGNNNCTG 2 cut(s) 2164, 4352
PsuI RGATCY 6 cut(s) 28, 620, 722, 1664, 2027, 3571
PvuI CGATCG 1 cut(s) 2656
PvuII CAGCTG 2 cut(s) 884, 1564
RseI CAYNNNNRTG 4 cut(s) 1639, 1960, 3275, 3790
SacI GAGCTC 3 cut(s) 1577, 3850, 4461
SapI GCTCTTC 2 cut(s) 1128, 3855
SaqAI TTAA 9 cut(s) 183, 324, 900, 1442, 2462, 2855, 2912, 3503, 4199
SbfI CCTGCAGG 1 cut(s) 4267
ScaI AGTACT 2 cut(s) 2796, 3018
ScrFI CCNGG 7 cut(s) 126, 1250, 1368, 1436, 1495, 2340, 3474
SdaI CCTGCAGG 1 cut(s) 4267
Sfr274I CTCGAG 1 cut(s) 3263
SfuI TTCGAA 1 cut(s) 2603
SinI GGWCC 6 cut(s) 432, 561, 759, 1371, 2336, 3994
SlaI CTCGAG 1 cut(s) 3263
SmiMI CAYNNNNRTG 4 cut(s) 1639, 1960, 3275, 3790
SmlI CTYRAG 9 cut(s) 323, 393, 1441, 1790, 1958, 3263, 3899, 4099, 4198
SmoI CTYRAG 9 cut(s) 323, 393, 1441, 1790, 1958, 3263, 3899, 4099, 4198
SnaBI TACGTA 2 cut(s) 1823, 3254
SpeI ACTAGT 3 cut(s) 863, 3401, 4333
SphI GCATGC 2 cut(s) 1257, 1553
Sse8387I CCTGCAGG 1 cut(s) 4267
SseBI AGGCCT 3 cut(s) 1241, 1439, 2289
SspI AATATT 2 cut(s) 351, 2095
SstI GAGCTC 3 cut(s) 1577, 3850, 4461
StuI AGGCCT 3 cut(s) 1241, 1439, 2289
StyD4I CCNGG 7 cut(s) 124, 1248, 1366, 1434, 1493, 2338, 3472
StyI CCWWGG 4 cut(s) 990, 2332, 2803, 2982
TaiI ACGT 9 cut(s) 742, 804, 1825, 2023, 2792, 3256, 3790, 4012, 4045
TaqI TCGA 9 cut(s) 423, 771, 999, 2603, 2652, 2684, 3264, 3464, 3696
TaqII GACCGA 2 cut(s) 320, 3423
TatI WGTACW 6 cut(s) 278, 847, 2794, 3016, 3247, 4235
Tru1I TTAA 9 cut(s) 183, 324, 900, 1442, 2462, 2855, 2912, 3503, 4199
Tru9I TTAA 9 cut(s) 183, 324, 900, 1442, 2462, 2855, 2912, 3503, 4199
TspGWI ACGGA 4 cut(s) 98, 1055, 2199, 3502
Van91I CCANNNNNTGG 2 cut(s) 575, 2396
Vha464I CTTAAG 3 cut(s) 323, 1441, 4198
VneI GTGCAC 1 cut(s) 1617
VpaK11BI GGWCC 6 cut(s) 432, 561, 759, 1371, 2336, 3994
XapI RAATTY 7 cut(s) 1310, 1804, 2037, 2548, 3046, 3355, 4132
XbaI TCTAGA 1 cut(s) 3497
XceI RCATGY 5 cut(s) 1257, 1553, 2051, 2614, 3197
XcmI CCANNNNNNNNNTGG 1 cut(s) 4561
XhoI CTCGAG 1 cut(s) 3263
XmiI GTMKAC 2 cut(s) 220, 3155
XmnI GAANNNNTTC 1 cut(s) 3624
ZrmI AGTACT 2 cut(s) 2796, 3018
Zsp2I ATGCAT 4 cut(s) 946, 1174, 1646, 1843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.