Rmu_ssc0000422.1_g000017

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000422.1
OG0019234
Physical Location & Seq
Forward (+)
72135 .. 76643
4509 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1389 bp
atgacgtttaagggttttagatggcttaaggccttgtggccgtctcggagatcaagtaggcttcatccgattgatgatggggttacaaatgtaaggaatttcattaccgtgaattcctttaacatggaaatagatgatgatccaattcccttccccgccgttgctataagctctaatcatgttcagaaaatgagaagtgcttgtttgaaaggcaaagaatgttctatatgcttggaggattttgagacgatcccaccctttttgattctcatcgggtccaactctcccaagggctactttaaagaatggcagattccgaggacattcagcctcttgtctgtgataatggtactggaatggttaaggctggatttgctggagatgatgccccaagggctgtcttccccaaaagacgcatatgttggggatgaggctcagtccaaaagaggtattctcactttaaaataccctattgaacatgggattgttagcaattgggacgacatggaaaagatctggcatcatactttctacaatgagcttcgtgtggctcctgaagaacatcctgtgctccttactgaggcacccctcaatcctaaagccaacagagagaagatgacacagatcatgtttgagaccttcaattcccctgctatgtatgttgccattcaagcagttctctccctctatgccagtgggcgtacaacaggtattgtgctcgactctggtgatggagtcagtcacacagttcccatctacgaaggttatgcacttccacatgccattctgcggcttgaccttgctggacgagatttaacagactccttaatgaagatcctgactgagagagggtattcttttactacaactgctgaacgggaaattgtacgtgacattaaagagaagcttgcatatgtggcacttgattatgaacaagaacttgagactgctgcaagcagctccacaattgagaagaactatgaactacctgatggtcaggtaattaccattggagcagagcgtttccgttgccctgaagtcctatttcaaccatcactagttggaatggaggcagctggaattcacgagaccacctataactcgattatgaagtgtgatgttgacatcaggaaagacttgtatggtaatattgtgctcagtggtgggtctactatgttcccaggaatcgctgataggatgagcaaggagataacagcccttgcaccaagcagtatgaagatcaaggtggtggcacctcctgagagaaagtacagtgtttggatcggaggatccatattggcttcactaagtacattccagcagatgtggatatctaaggcagagtacgatgaatccggcccatccatcgtccacagaaaatgcttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

462

Amino Acids

51.97

Weight (kDa)

5.59

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 138 - 462 1.9e-129 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019234)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g10191 FvH4_5g10192
prunus_persica Prupe.5G137000_v2.0.a1
rosa_multiflora Rmu_ssc0000422.1_g000017
rosa_samantha Rh7AG092000 Rh7BG093600 Rh7CG092900 Rh7DG094100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 583, 1252
AccI GTMKAC 1 cut(s) 1169
AciI CCGC 2 cut(s) 156, 790
AclWI GGATC 6 cut(s) 134, 244, 829, 1284, 1289, 1297
AcoI YGGCCR 1 cut(s) 38
AcsI RAATTY 3 cut(s) 97, 112, 1080
AcuI CTGAAG 2 cut(s) 576, 1056
AfaI GTAC 6 cut(s) 351, 703, 888, 1271, 1312, 1346
AfiI CCNNNNNNNGG 2 cut(s) 580, 789
AflII CTTAAG 1 cut(s) 26
AgsI TTSAA 5 cut(s) 208, 476, 643, 671, 1049
AhlI ACTAGT 1 cut(s) 1057
AjnI CCWGG 1 cut(s) 1180
AluBI AGCT 5 cut(s) 171, 541, 907, 960, 1076
AluI AGCT 5 cut(s) 171, 541, 907, 960, 1076
Alw21I GWGCWC 3 cut(s) 573, 720, 1158
Alw26I GTCTC 5 cut(s) 48, 239, 629, 938, 1082
AlwI GGATC 6 cut(s) 134, 244, 829, 1284, 1289, 1297
AoxI GGCC 3 cut(s) 30, 38, 1357
ApeKI GCWGC 3 cut(s) 950, 957, 1073
ApoI RAATTY 3 cut(s) 97, 112, 1080
AspS9I GGNCC 2 cut(s) 276, 1358
AsuHPI GGTGA 1 cut(s) 740
AvaII GGWCC 1 cut(s) 276
BamHI GGATCC 1 cut(s) 1289
BanI GGYRCC 2 cut(s) 583, 1252
BauI CACGAG 1 cut(s) 1085
BbsI GAAGAC 1 cut(s) 393
Bbv12I GWGCWC 3 cut(s) 573, 720, 1158
BbvI GCAGC 3 cut(s) 937, 969, 1085
BccI CCATC 8 cut(s) 15, 71, 725, 761, 986, 1060, 1369, 1373
BceAI ACGGC 2 cut(s) 25, 143
BcgI CGANNNNNNTGC 2 cut(s) 749, 783
BciT130I CCWGG 1 cut(s) 1182
BcoDI GTCTC 5 cut(s) 48, 239, 629, 938, 1082
BcuI ACTAGT 1 cut(s) 1057
BfaI CTAG 2 cut(s) 1058, 1387
BfrI CTTAAG 1 cut(s) 26
BglI GCCNNNNNGGC 1 cut(s) 394
BglII AGATCT 1 cut(s) 513
BisI GCNGC 4 cut(s) 791, 951, 958, 1074
BlsI GCNGC 4 cut(s) 792, 952, 959, 1075
Bme1390I CCNGG 1 cut(s) 1182
Bme18I GGWCC 1 cut(s) 276
BmgT120I GGNCC 2 cut(s) 276, 1358
BmiI GGNNCC 5 cut(s) 277, 552, 585, 1254, 1291
BmrFI CCNGG 1 cut(s) 1182
BmsI GCATC 2 cut(s) 375, 529
BpiI GAAGAC 1 cut(s) 393
BplI GAGNNNNNCTC 2 cut(s) 438, 470
BpmI CTGGAG 1 cut(s) 398
BpuEI CTTGAG 1 cut(s) 962
BsaAI YACGTR 1 cut(s) 890
BsaBI GATNNNNATC 2 cut(s) 138, 269
BsaI GGTCTC 2 cut(s) 629, 1082
BsaJI CCNNGG 4 cut(s) 288, 317, 391, 1180
BsaXI ACNNNNNCTCC 2 cut(s) 40, 70
Bsc4I CCNNNNNNNGG 2 cut(s) 580, 789
Bse1I ACTGG 2 cut(s) 357, 693
Bse8I GATNNNNATC 2 cut(s) 138, 269
BseBI CCWGG 1 cut(s) 1182
BseDI CCNNGG 4 cut(s) 288, 317, 391, 1180
BseGI GGATG 5 cut(s) 64, 433, 562, 1203, 1361
BseJI GATNNNNATC 2 cut(s) 138, 269
BseLI CCNNNNNNNGG 2 cut(s) 580, 789
BseMII CTCAG 5 cut(s) 449, 570, 834, 1171, 1251
BseNI ACTGG 2 cut(s) 357, 693
BseXI GCAGC 3 cut(s) 937, 969, 1085
BshFI GGCC 3 cut(s) 32, 40, 1359
BshNI GGYRCC 2 cut(s) 583, 1252
BsiHKAI GWGCWC 3 cut(s) 573, 720, 1158
BsiSI CCGG 1 cut(s) 1356
BslFI GGGAC 1 cut(s) 512
BslI CCNNNNNNNGG 2 cut(s) 580, 789
BsmAI GTCTC 5 cut(s) 48, 239, 629, 938, 1082
BsmBI CGTCTC 2 cut(s) 48, 239
BsmFI GGGAC 1 cut(s) 512
BsnI GGCC 3 cut(s) 32, 40, 1359
Bso31I GGTCTC 2 cut(s) 629, 1082
Bsp1286I GDGCHC 3 cut(s) 573, 720, 1158
Bsp143I GATC 9 cut(s) 50, 139, 249, 513, 624, 834, 1239, 1281, 1289
BspACI CCGC 2 cut(s) 156, 790
BspANI GGCC 3 cut(s) 32, 40, 1359
BspCNI CTCAG 5 cut(s) 448, 571, 835, 1170, 1252
BspLI GGNNCC 5 cut(s) 277, 552, 585, 1254, 1291
BspPI GGATC 6 cut(s) 134, 244, 829, 1284, 1289, 1297
BspT107I GGYRCC 2 cut(s) 583, 1252
BspTI CTTAAG 1 cut(s) 26
BspTNI GGTCTC 2 cut(s) 629, 1082
BsrI ACTGG 2 cut(s) 357, 693
BssECI CCNNGG 4 cut(s) 288, 317, 391, 1180
BssMI GATC 9 cut(s) 50, 139, 249, 513, 624, 834, 1239, 1281, 1289
BssSI CACGAG 1 cut(s) 1085
BssT1I CCWWGG 2 cut(s) 288, 391
Bst2BI CACGAG 1 cut(s) 1085
Bst2UI CCWGG 1 cut(s) 1182
Bst4CI ACNGT 3 cut(s) 109, 748, 1274
BstAFI CTTAAG 1 cut(s) 26
BstBAI YACGTR 1 cut(s) 890
BstC8I GCNNGC 2 cut(s) 909, 955
BstDEI CTNAG 7 cut(s) 435, 579, 843, 1157, 1260, 1307, 1335
BstENI CCTNNNNNAGG 1 cut(s) 578
BstF5I GGATG 5 cut(s) 64, 433, 562, 1203, 1361
BstKTI GATC 9 cut(s) 53, 142, 252, 516, 627, 837, 1242, 1284, 1292
BstMAI GTCTC 5 cut(s) 48, 239, 629, 938, 1082
BstMBI GATC 9 cut(s) 50, 139, 249, 513, 624, 834, 1239, 1281, 1289
BstMWI GCNNNNNNNGC 4 cut(s) 373, 394, 671, 917
BstNI CCWGG 1 cut(s) 1182
BstNSI RCATGY 1 cut(s) 782
BstSCI CCNGG 1 cut(s) 1180
BstV1I GCAGC 3 cut(s) 937, 969, 1085
BstV2I GAAGAC 1 cut(s) 393
BstX2I RGATCY 3 cut(s) 513, 834, 1289
BstYI RGATCY 3 cut(s) 513, 834, 1289
BsuRI GGCC 3 cut(s) 32, 40, 1359
BtsCI GGATG 5 cut(s) 64, 433, 562, 1203, 1361
BtsIMutI CAGTG 3 cut(s) 700, 1165, 1279
Cac8I GCNNGC 2 cut(s) 909, 955
Cfr13I GGNCC 2 cut(s) 276, 1358
CseI GACGC 1 cut(s) 422
Csp6I GTAC 6 cut(s) 350, 702, 887, 1270, 1311, 1345
CspCI CAANNNNNGTGG 2 cut(s) 243, 278
CviAII CATG 6 cut(s) 124, 179, 479, 505, 628, 779
CviQI GTAC 6 cut(s) 350, 702, 887, 1270, 1311, 1345
DdeI CTNAG 7 cut(s) 435, 579, 843, 1157, 1260, 1307, 1335
DpnI GATC 9 cut(s) 52, 141, 251, 515, 626, 836, 1241, 1283, 1291
DpnII GATC 9 cut(s) 50, 139, 249, 513, 624, 834, 1239, 1281, 1289
DraI TTTAAA 2 cut(s) 301, 462
EaeI YGGCCR 1 cut(s) 38
Eco130I CCWWGG 2 cut(s) 288, 391
Eco147I AGGCCT 1 cut(s) 32
Eco31I GGTCTC 2 cut(s) 629, 1082
Eco32I GATATC 1 cut(s) 1332
Eco47I GGWCC 1 cut(s) 276
Eco57I CTGAAG 2 cut(s) 576, 1056
EcoNI CCTNNNNNAGG 1 cut(s) 578
EcoRI GAATTC 2 cut(s) 112, 1080
EcoRII CCWGG 1 cut(s) 1180
EcoRV GATATC 1 cut(s) 1332
EcoT14I CCWWGG 2 cut(s) 288, 391
ErhI CCWWGG 2 cut(s) 288, 391
Esp3I CGTCTC 2 cut(s) 48, 239
FaeI CATG 6 cut(s) 127, 182, 482, 508, 631, 782
FalI AAGNNNNNCTT 4 cut(s) 281, 313, 891, 923
FaqI GGGAC 1 cut(s) 512
FatI CATG 6 cut(s) 123, 178, 478, 504, 627, 778
FauI CCCGC 1 cut(s) 163
FauNDI CATATG 2 cut(s) 418, 913
FblI GTMKAC 1 cut(s) 1169
Fnu4HI GCNGC 4 cut(s) 791, 951, 958, 1074
FokI GGATG 5 cut(s) 51, 440, 549, 1210, 1348
Fsp4HI GCNGC 4 cut(s) 791, 951, 958, 1074
FspBI CTAG 2 cut(s) 1058, 1387
GluI GCNGC 4 cut(s) 791, 951, 958, 1074
GsuI CTGGAG 1 cut(s) 398
HaeIII GGCC 3 cut(s) 32, 40, 1359
HapII CCGG 1 cut(s) 1356
HgaI GACGC 1 cut(s) 422
Hin1II CATG 6 cut(s) 127, 182, 482, 508, 631, 782
HincII GTYRAC 1 cut(s) 1123
HindII GTYRAC 1 cut(s) 1123
HindIII AAGCTT 1 cut(s) 905
HinfI GANTC 7 cut(s) 265, 313, 722, 735, 821, 1185, 1352
HpaII CCGG 1 cut(s) 1356
HphI GGTGA 1 cut(s) 740
Hpy166II GTNNAC 3 cut(s) 1123, 1170, 1372
Hpy188I TCNGA 5 cut(s) 48, 69, 186, 318, 1286
Hpy188III TCNNGA 5 cut(s) 554, 838, 1085, 1129, 1259
Hpy8I GTNNAC 3 cut(s) 1123, 1170, 1372
HpyAV CCTTC 3 cut(s) 160, 649, 755
HpyCH4III ACNGT 3 cut(s) 109, 748, 1274
HpyCH4IV ACGT 2 cut(s) 5, 889
HpyCH4V TGCA 4 cut(s) 770, 911, 953, 1223
HpyF10VI GCNNNNNNNGC 4 cut(s) 373, 394, 671, 917
HpyF3I CTNAG 7 cut(s) 435, 579, 843, 1157, 1260, 1307, 1335
HpySE526I ACGT 2 cut(s) 5, 889
Hsp92II CATG 6 cut(s) 127, 182, 482, 508, 631, 782
Kzo9I GATC 9 cut(s) 50, 139, 249, 513, 624, 834, 1239, 1281, 1289
LmnI GCTCC 4 cut(s) 556, 576, 965, 1013
Lsp1109I GCAGC 3 cut(s) 937, 969, 1085
LweI GCATC 2 cut(s) 375, 529
MaeI CTAG 2 cut(s) 1058, 1387
MaeII ACGT 2 cut(s) 5, 889
MaeIII GTNAC 3 cut(s) 82, 740, 890
MalI GATC 9 cut(s) 52, 141, 251, 515, 626, 836, 1241, 1283, 1291
MboI GATC 9 cut(s) 50, 139, 249, 513, 624, 834, 1239, 1281, 1289
MboII GAAGA 6 cut(s) 393, 569, 625, 844, 985, 1249
MfeI CAATTG 2 cut(s) 493, 966
MflI RGATCY 3 cut(s) 513, 834, 1289
MhlI GDGCHC 3 cut(s) 573, 720, 1158
MluCI AATT 9 cut(s) 97, 112, 144, 493, 643, 882, 966, 1002, 1080
MlyI GAGTC 3 cut(s) 716, 744, 815
MmeI TCCRAC 2 cut(s) 303, 1042
MseI TTAA 9 cut(s) 9, 27, 120, 300, 362, 461, 815, 827, 897
MslI CAYNNNNRTG 1 cut(s) 107
MspA1I CMGCKG 1 cut(s) 1076
MspCI CTTAAG 1 cut(s) 26
MspI CCGG 1 cut(s) 1356
MspR9I CCNGG 1 cut(s) 1182
MunI CAATTG 2 cut(s) 493, 966
MvaI CCWGG 1 cut(s) 1182
MwoI GCNNNNNNNGC 4 cut(s) 373, 394, 671, 917
NdeI CATATG 2 cut(s) 418, 913
NdeII GATC 9 cut(s) 50, 139, 249, 513, 624, 834, 1239, 1281, 1289
NlaIII CATG 6 cut(s) 127, 182, 482, 508, 631, 782
NlaIV GGNNCC 5 cut(s) 277, 552, 585, 1254, 1291
NmuCI GTSAC 2 cut(s) 740, 890
NspI RCATGY 1 cut(s) 782
PceI AGGCCT 1 cut(s) 32
PfeI GAWTC 4 cut(s) 265, 313, 1185, 1352
PkrI GCNGC 4 cut(s) 792, 952, 959, 1075
PleI GAGTC 3 cut(s) 716, 743, 815
PpsI GAGTC 3 cut(s) 716, 743, 815
Ppu21I YACGTR 1 cut(s) 890
Psp6I CCWGG 1 cut(s) 1180
PspGI CCWGG 1 cut(s) 1180
PspN4I GGNNCC 5 cut(s) 277, 552, 585, 1254, 1291
PspPI GGNCC 2 cut(s) 276, 1358
PsuI RGATCY 3 cut(s) 513, 834, 1289
PvuII CAGCTG 1 cut(s) 1076
RsaI GTAC 6 cut(s) 351, 703, 888, 1271, 1312, 1346
RsaNI GTAC 6 cut(s) 350, 702, 887, 1270, 1311, 1345
RseI CAYNNNNRTG 1 cut(s) 107
SaqAI TTAA 9 cut(s) 9, 27, 120, 300, 362, 461, 815, 827, 897
SatI GCNGC 4 cut(s) 791, 951, 958, 1074
Sau3AI GATC 9 cut(s) 50, 139, 249, 513, 624, 834, 1239, 1281, 1289
Sau96I GGNCC 2 cut(s) 276, 1358
SchI GAGTC 3 cut(s) 716, 744, 815
ScrFI CCNGG 1 cut(s) 1182
SduI GDGCHC 3 cut(s) 573, 720, 1158
SfaNI GCATC 2 cut(s) 375, 529
SinI GGWCC 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 2 cut(s) 26, 941
SmoI CTYRAG 2 cut(s) 26, 941
SpeI ACTAGT 1 cut(s) 1057
Sse9I AATT 9 cut(s) 97, 112, 144, 493, 643, 882, 966, 1002, 1080
SseBI AGGCCT 1 cut(s) 32
SsiI CCGC 2 cut(s) 156, 790
SspI AATATT 1 cut(s) 1150
SspMI CTAG 2 cut(s) 1058, 1387
StuI AGGCCT 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 1180
StyI CCWWGG 2 cut(s) 288, 391
TaaI ACNGT 3 cut(s) 109, 748, 1274
TaiI ACGT 2 cut(s) 8, 892
TaqI TCGA 2 cut(s) 720, 1103
TasI AATT 9 cut(s) 97, 112, 144, 493, 643, 882, 966, 1002, 1080
TatI WGTACW 2 cut(s) 1269, 1310
TauI GCSGC 1 cut(s) 793
TfiI GAWTC 4 cut(s) 265, 313, 1185, 1352
Tru1I TTAA 9 cut(s) 9, 27, 120, 300, 362, 461, 815, 827, 897
Tru9I TTAA 9 cut(s) 9, 27, 120, 300, 362, 461, 815, 827, 897
TscAI CASTG 3 cut(s) 700, 1165, 1279
TseFI GTSAC 2 cut(s) 740, 890
TseI GCWGC 3 cut(s) 950, 957, 1073
Tsp45I GTSAC 2 cut(s) 740, 890
TspDTI ATGAA 8 cut(s) 53, 91, 845, 945, 996, 1124, 1250, 1365
TspGWI ACGGA 1 cut(s) 1016
TspRI CASTG 3 cut(s) 700, 1165, 1279
Vha464I CTTAAG 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 276
XagI CCTNNNNNAGG 1 cut(s) 578
XapI RAATTY 3 cut(s) 97, 112, 1080
XceI RCATGY 1 cut(s) 782
XmiI GTMKAC 1 cut(s) 1169
XspI CTAG 2 cut(s) 1058, 1387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.