Rroxscaffold_1G00012460
ERF Family

mitochondrial transcription termination factor family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0022456
Physical Location & Seq
Forward (+)
15348642 .. 15349620
979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00012460.1

Sequence Viewer

Length: 576 bp
ATGAAGCCGGTACCGATCGAGCTGTTGGGAGGAAGGACTGCGGCTCCCACAGCTGCAAAGTTAACACCATGCATGTTTGAAGTGGACTTCAATGGCGTTCATATATGTACAGGTCCTGAAACGAAAGCCTCAGTCTTGAGCTGCCAAGTTCTGAAGAGATTTGGAAGAGAAAGGGAAAAAAGACTTGATTTTGTCCAAAGTGATCTTTGGATCGTACAGCTCAAAGTGATCTTTGGATCAGCCTATACTAACAAGCTAATATCAACAGATGTGGATTTCCTTCCACTTATAGCTAATATCAACAAAAAGCTCTACCACCAGACCCGTCAGCCTCTATACAGACAGACTTCTCTTCGAGATCTACGTTGTTTTGTTATAAGTTCGATACATCACAGGACTACCATTATTGCATTGTTATCTCAAGATAAACATTTGATACTTATAATTGTAGGGCTTTACCCCGAAGGGAGCCCTTCCAGAAATTCTCAAGGGCACAAGGCTGAACCCAGGCCCAGGAGGGGGTTTGGAAATAGTTTTCAACCAGCAATAGACGCGCCTCGGTTAGAACCTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
mTERF

Protein Analysis

191

Amino Acids

21.54

Weight (kDa)

9.76

Isoelectric Point (pI)

50.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022456)

Species Orthologous Gene IDs
prunus_persica Prupe.8G171200_v2.0.a1 Prupe.8G171500_v2.0.a1
pyrus_communis pycom01g15660
rosa_roxburghii Rroxscaffold_1G00012460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 377, 443
Acc65I GGTACC 1 cut(s) 10
AccB1I GGYRCC 1 cut(s) 10
AccII CGCG 1 cut(s) 554
AciI CCGC 1 cut(s) 41
AclWI GGATC 2 cut(s) 218, 244
AcsI RAATTY 1 cut(s) 481
AcuI CTGAAG 1 cut(s) 173
AfaI GTAC 3 cut(s) 12, 109, 216
AfiI CCNNNNNNNGG 3 cut(s) 513, 518, 519
AgsI TTSAA 3 cut(s) 80, 91, 539
AjnI CCWGG 2 cut(s) 506, 512
AluBI AGCT 7 cut(s) 22, 53, 141, 220, 256, 293, 310
AluI AGCT 7 cut(s) 22, 53, 141, 220, 256, 293, 310
AlwI GGATC 2 cut(s) 218, 244
AlwNI CAGNNNCTG 1 cut(s) 116
AoxI GGCC 1 cut(s) 509
ApeKI GCWGC 2 cut(s) 53, 141
ApoI RAATTY 1 cut(s) 481
Asp718I GGTACC 1 cut(s) 10
AspLEI GCGC 1 cut(s) 556
AspS9I GGNCC 2 cut(s) 113, 510
AvaII GGWCC 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 495
BanI GGYRCC 1 cut(s) 10
BanII GRGCYC 1 cut(s) 473
BbvI GCAGC 2 cut(s) 40, 128
BciT130I CCWGG 2 cut(s) 508, 514
BglII AGATCT 1 cut(s) 358
BisI GCNGC 3 cut(s) 42, 54, 142
BlsI GCNGC 3 cut(s) 43, 55, 143
Bme1390I CCNGG 2 cut(s) 508, 514
Bme18I GGWCC 1 cut(s) 113
BmgT120I GGNCC 2 cut(s) 113, 510
BmiI GGNNCC 3 cut(s) 12, 45, 470
BmrFI CCNGG 2 cut(s) 508, 514
BpuEI CTTGAG 3 cut(s) 157, 405, 471
BsaJI CCNNGG 3 cut(s) 506, 512, 557
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bsc4I CCNNNNNNNGG 3 cut(s) 513, 518, 519
Bse118I RCCGGY 1 cut(s) 7
BseBI CCWGG 2 cut(s) 508, 514
BseDI CCNNGG 3 cut(s) 506, 512, 557
BseLI CCNNNNNNNGG 3 cut(s) 513, 518, 519
BseMII CTCAG 1 cut(s) 144
BseSI GKGCMC 1 cut(s) 495
BseXI GCAGC 2 cut(s) 40, 128
Bsh1236I CGCG 1 cut(s) 554
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 1 cut(s) 511
BshNI GGYRCC 1 cut(s) 10
BsiEI CGRYCG 1 cut(s) 18
BsiSI CCGG 1 cut(s) 8
BslI CCNNNNNNNGG 3 cut(s) 513, 518, 519
BsnI GGCC 1 cut(s) 511
Bsp1286I GDGCHC 2 cut(s) 473, 495
Bsp1407I TGTACA 1 cut(s) 107
Bsp143I GATC 6 cut(s) 15, 202, 210, 228, 236, 358
BspACI CCGC 1 cut(s) 41
BspANI GGCC 1 cut(s) 511
BspCNI CTCAG 1 cut(s) 143
BspFNI CGCG 1 cut(s) 554
BspLI GGNNCC 3 cut(s) 12, 45, 470
BspPI GGATC 2 cut(s) 218, 244
BspT107I GGYRCC 1 cut(s) 10
BsrFI RCCGGY 1 cut(s) 7
BsrGI TGTACA 1 cut(s) 107
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 3 cut(s) 506, 512, 557
BssMI GATC 6 cut(s) 15, 202, 210, 228, 236, 358
Bst2UI CCWGG 2 cut(s) 508, 514
Bst6I CTCTTC 3 cut(s) 149, 160, 357
BstAUI TGTACA 1 cut(s) 107
BstDEI CTNAG 1 cut(s) 130
BstFNI CGCG 1 cut(s) 554
BstHHI GCGC 1 cut(s) 556
BstKTI GATC 6 cut(s) 18, 205, 213, 231, 239, 361
BstMBI GATC 6 cut(s) 15, 202, 210, 228, 236, 358
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 2 cut(s) 50, 551
BstNI CCWGG 2 cut(s) 508, 514
BstNSI RCATGY 1 cut(s) 76
BstSCI CCNGG 2 cut(s) 506, 512
BstSLI GKGCMC 1 cut(s) 495
BstUI CGCG 1 cut(s) 554
BstV1I GCAGC 2 cut(s) 40, 128
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuRI GGCC 1 cut(s) 511
CaiI CAGNNNCTG 1 cut(s) 116
CfoI GCGC 1 cut(s) 556
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 113, 510
CseI GACGC 1 cut(s) 560
Csp6I GTAC 3 cut(s) 11, 108, 215
CspCI CAANNNNNGTGG 2 cut(s) 252, 287
CviAII CATG 2 cut(s) 69, 73
CviQI GTAC 3 cut(s) 11, 108, 215
DdeI CTNAG 1 cut(s) 130
DpnI GATC 6 cut(s) 17, 204, 212, 230, 238, 360
DpnII GATC 6 cut(s) 15, 202, 210, 228, 236, 358
Eam1104I CTCTTC 3 cut(s) 149, 160, 357
EarI CTCTTC 3 cut(s) 149, 160, 357
Eco24I GRGCYC 1 cut(s) 473
Eco47I GGWCC 1 cut(s) 113
Eco57I CTGAAG 1 cut(s) 173
EcoO109I RGGNCCY 1 cut(s) 113
EcoRII CCWGG 2 cut(s) 506, 512
EcoT22I ATGCAT 1 cut(s) 74
EcoT38I GRGCYC 1 cut(s) 473
FaeI CATG 2 cut(s) 72, 76
FatI CATG 2 cut(s) 68, 72
Fnu4HI GCNGC 3 cut(s) 42, 54, 142
FriOI GRGCYC 1 cut(s) 473
Fsp4HI GCNGC 3 cut(s) 42, 54, 142
GlaI GCGC 1 cut(s) 555
GluI GCNGC 3 cut(s) 42, 54, 142
HaeIII GGCC 1 cut(s) 511
HapII CCGG 1 cut(s) 8
HgaI GACGC 1 cut(s) 560
HhaI GCGC 1 cut(s) 556
Hin1II CATG 2 cut(s) 72, 76
Hin6I GCGC 1 cut(s) 554
HinP1I GCGC 1 cut(s) 554
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HpaI GTTAAC 1 cut(s) 63
HpaII CCGG 1 cut(s) 8
Hpy166II GTNNAC 2 cut(s) 63, 85
Hpy188I TCNGA 1 cut(s) 153
Hpy188III TCNNGA 5 cut(s) 116, 136, 356, 422, 477
Hpy8I GTNNAC 2 cut(s) 63, 85
HpyAV CCTTC 4 cut(s) 27, 290, 458, 483
HpyCH4IV ACGT 1 cut(s) 364
HpyCH4V TGCA 3 cut(s) 56, 72, 410
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 551
HpyF3I CTNAG 1 cut(s) 130
HpySE526I ACGT 1 cut(s) 364
Hsp92II CATG 2 cut(s) 72, 76
HspAI GCGC 1 cut(s) 554
KpnI GGTACC 1 cut(s) 14
KspAI GTTAAC 1 cut(s) 63
Kzo9I GATC 6 cut(s) 15, 202, 210, 228, 236, 358
LmnI GCTCC 2 cut(s) 49, 468
Lsp1109I GCAGC 2 cut(s) 40, 128
MaeII ACGT 1 cut(s) 364
MalI GATC 6 cut(s) 17, 204, 212, 230, 238, 360
MboI GATC 6 cut(s) 15, 202, 210, 228, 236, 358
MboII GAAGA 3 cut(s) 166, 177, 344
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 2 cut(s) 473, 495
MluCI AATT 2 cut(s) 444, 481
MnlI CCTC 5 cut(s) 23, 139, 342, 510, 567
Mph1103I ATGCAT 1 cut(s) 74
MseI TTAA 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 1 cut(s) 8
MspR9I CCNGG 2 cut(s) 508, 514
MvaI CCWGG 2 cut(s) 508, 514
MvnI CGCG 1 cut(s) 554
MwoI GCNNNNNNNGC 2 cut(s) 50, 551
NdeII GATC 6 cut(s) 15, 202, 210, 228, 236, 358
NlaIII CATG 2 cut(s) 72, 76
NlaIV GGNNCC 3 cut(s) 12, 45, 470
NsiI ATGCAT 1 cut(s) 74
NspI RCATGY 1 cut(s) 76
PcsI WCGNNNNNNNCGW 1 cut(s) 361
PkrI GCNGC 3 cut(s) 43, 55, 143
Ple19I CGATCG 1 cut(s) 18
PpuMI RGGWCCY 1 cut(s) 113
PsiI TTATAA 2 cut(s) 377, 443
Psp5II RGGWCCY 1 cut(s) 113
Psp6I CCWGG 2 cut(s) 506, 512
PspGI CCWGG 2 cut(s) 506, 512
PspN4I GGNNCC 3 cut(s) 12, 45, 470
PspPI GGNCC 2 cut(s) 113, 510
PspPPI RGGWCCY 1 cut(s) 113
PstNI CAGNNNCTG 1 cut(s) 116
PsuI RGATCY 1 cut(s) 358
PvuI CGATCG 1 cut(s) 18
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 3 cut(s) 12, 109, 216
RsaNI GTAC 3 cut(s) 11, 108, 215
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 3 cut(s) 42, 54, 142
Sau3AI GATC 6 cut(s) 15, 202, 210, 228, 236, 358
Sau96I GGNCC 2 cut(s) 113, 510
ScrFI CCNGG 2 cut(s) 508, 514
SduI GDGCHC 2 cut(s) 473, 495
SinI GGWCC 1 cut(s) 113
SmlI CTYRAG 3 cut(s) 136, 420, 486
SmoI CTYRAG 3 cut(s) 136, 420, 486
Sse9I AATT 2 cut(s) 444, 481
SsiI CCGC 1 cut(s) 41
StyD4I CCNGG 2 cut(s) 506, 512
TaiI ACGT 1 cut(s) 367
TaqI TCGA 3 cut(s) 18, 355, 383
TasI AATT 2 cut(s) 444, 481
TatI WGTACW 1 cut(s) 107
TauI GCSGC 1 cut(s) 44
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TseI GCWGC 2 cut(s) 53, 141
TspDTI ATGAA 2 cut(s) 17, 89
VpaK11BI GGWCC 1 cut(s) 113
XapI RAATTY 1 cut(s) 481
XceI RCATGY 1 cut(s) 76
Zsp2I ATGCAT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.