Rroxscaffold_1G00019650

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0019082
Physical Location & Seq
Forward (+)
24006192 .. 24007655
1464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00019650.1

Sequence Viewer

Length: 1464 bp
ATGGACGACAATGAAGACGAACAGACAATTTCTCTTCCTCCCAAGAAGAGACTGAAACAACATGATCGTCGTCAGCCTAATCATGAAGGCAGCAGCAGTGAAATCCACTCTTGCAATGTCTGCCAGAAAAGCTTCGCATCACTCAAGGCTTTGAACGGCCACATGAAAATCCACGGCGGAAGCAAATCCAAGGCTGCCAGGCCTCCGTTTCCGGCACCGGAACCATCTGAAGACAACGACAACGACAATGACAATGATGGTGTTAGTGCTAGTAGTTCATCCGATACCAACATAAAGAAAGATGTTGATGTAGCAAGCTTCTTGCAAAACTTGAGCGATATCGAAATTCAGAGGAACGGGCCAAACGGGCCAAGGGAAGAAGGGTATTGTTGGAAGCAATGCAGTAGAGATTACAATGCAGAAGACCGGCTTGGTTATGGAATTTCAGGGAAGAAGACAATGCCGGTTCCATACAAGGACAACCCTTCATCCAAGAAAGTGAAAATTGATGGTACAACTAGAACTGGTTCTGCTTTGGTACAGAAGAAGTTGGCCGGAGGTAAAAGAAAGGCTTCTGTGGAAGATGATGAGAAAGGTGATCAAAGCAGAGTACCAAAAGATTTCACAAGTAATATTTCAAAGAAGGTAAAGATTGTTGGCAGAGGTGAAGACCCCAAAAGAGACATGGTTTCACATGACCAAACCCGGAAGCTGCAGATTCCAAGAAAGCCCCAAGTTGAGTCAAGTAAAACCTTCCCAGCTTCACAAGCAATGGAGACTTACAGCTGGAATTGTAAAAGTAGCAGATCGGCTAGTGCGAGTGTGAAAATAGGTGAATCTGGGTTTGGTACTAAGTATGCTGCAAAGAGTAGTACGAGTTTGAAAATGGGTGAATCTGGGTTTGGTGCCAAGTATGGTACAAAGAGTAATACGAATGTAAAAATGGGTCATTCCGGGTATGGTACCAAGTATGCAAAGAGTACTACGAGTGTAAAAGTGGGTGAATCTGGGTTTGGTACTAAGTTGGCTGCTCGTGCTCCGACTAGTACTAAAGAAGCTGCAAAGGAGGATAACAAACAGCATGTGTGCGAAATTTGCCACAGGACGTTTAAGACTTATCAGGGTCTTGGTGGCCACAGGAGGATTCATAATTCGAGAGGAGGAGTGGCGAAAGAAGCTCCTACAGCACCTGAGGTCAGAGCAGCGACAGGTCATGAAGATGGTGAGACTGGTGTAGACCCTCAAGGCCACAGGAGTGTTGGCAGTTCTAGAAGAGTATCAGTGAGAAAAGCTCCTGCTGCACCTGTGGTTAGAGCACCGTCTGATCATGAAGATGGTGGGAGTGATGAAGACCCTGAAGCAGCATTGCATGAAGAAGAAGCAGGGGCCAAAACTGAAGGGCGCCATGAAAGAGGTTTCAATCTCAACCAAGTCCCAGATGAGGATTCGGATGGAGCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C2H2_6

Protein Analysis

487

Amino Acids

52.58

Weight (kDa)

9.01

Isoelectric Point (pI)

49.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_6 PF13912 36 - 58 2.4e-07 C2H2-type zinc finger
zf-C2H2_6 PF13912 361 - 383 1.8e-07 C2H2-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019082)

Species Orthologous Gene IDs
rosa_laevigata RLG00000035419
rosa_multiflora Rmu_sc0000640.1_g000012
rosa_roxburghii Rroxscaffold_1G00019650
rosa_rugosa Rorug05G0335800
rosa_samantha Rh5AG398300 Rh5BG411200 Rh5CG436200 Rh5DG425600
rosa_wichuraiana Rw5G037550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 962
AccB1I GGYRCC 4 cut(s) 214, 905, 962, 1401
AccI GTMKAC 1 cut(s) 1236
AciI CCGC 1 cut(s) 177
AcoI YGGCCR 3 cut(s) 157, 552, 1132
AcsI RAATTY 3 cut(s) 345, 441, 1092
AcuI CTGAAG 3 cut(s) 249, 1377, 1416
AcyI GRCGYC 1 cut(s) 1402
AfiI CCNNNNNNNGG 1 cut(s) 1441
AgsI TTSAA 4 cut(s) 154, 639, 883, 1420
AhlI ACTAGT 1 cut(s) 1043
AjnI CCWGG 1 cut(s) 197
AjuI GAANNNNNNNTTGG 8 cut(s) 414, 446, 828, 860, 885, 917, 996, 1028
AleI CACNNNNGTG 1 cut(s) 1254
AluBI AGCT 9 cut(s) 132, 318, 712, 761, 786, 1058, 1178, 1292, 1457
AluI AGCT 9 cut(s) 132, 318, 712, 761, 786, 1058, 1178, 1292, 1457
Alw21I GWGCWC 2 cut(s) 1039, 1318
Alw26I GTCTC 4 cut(s) 43, 675, 770, 1220
AlwNI CAGNNNCTG 1 cut(s) 1190
AoxI GGCC 8 cut(s) 157, 200, 359, 368, 552, 1132, 1246, 1386
ApoI RAATTY 3 cut(s) 345, 441, 1092
Asp700I GAANNNNTTC 3 cut(s) 131, 526, 571
Asp718I GGTACC 1 cut(s) 962
AspLEI GCGC 1 cut(s) 1404
AspS9I GGNCC 3 cut(s) 359, 368, 1386
AsuC2I CCSGG 2 cut(s) 706, 955
AsuHPI GGTGA 6 cut(s) 608, 677, 845, 902, 1013, 1235
AxyI CCTNAGG 1 cut(s) 1191
BalI TGGCCA 1 cut(s) 1134
BanI GGYRCC 4 cut(s) 214, 905, 962, 1401
BauI CACGAG 1 cut(s) 1032
BbsI GAAGAC 6 cut(s) 21, 237, 429, 461, 675, 1356
Bbv12I GWGCWC 2 cut(s) 1039, 1318
BccI CCATC 6 cut(s) 232, 251, 503, 1214, 1328, 1445
BceAI ACGGC 2 cut(s) 172, 190
BciT130I CCWGG 1 cut(s) 199
BclI TGATCA 2 cut(s) 598, 1324
BcnI CCSGG 2 cut(s) 706, 955
BcoDI GTCTC 4 cut(s) 43, 675, 770, 1220
BcuI ACTAGT 1 cut(s) 1043
BfaI CTAG 5 cut(s) 270, 519, 813, 1044, 1269
BfmI CTRYAG 2 cut(s) 713, 1182
BfoI RGCGCY 1 cut(s) 1405
BglI GCCNNNNNGGC 1 cut(s) 367
BmcAI AGTACT 2 cut(s) 982, 1048
Bme1390I CCNGG 3 cut(s) 199, 706, 955
BmgT120I GGNCC 3 cut(s) 359, 368, 1386
BmiI GGNNCC 7 cut(s) 216, 222, 468, 907, 964, 1387, 1403
BmrFI CCNGG 3 cut(s) 199, 706, 955
BmsI GCATC 1 cut(s) 146
BpiI GAAGAC 6 cut(s) 21, 237, 429, 461, 675, 1356
BplI GAGNNNNNCTC 2 cut(s) 1276, 1308
BpuEI CTTGAG 3 cut(s) 128, 352, 1227
BpuMI CCSGG 2 cut(s) 706, 955
BsaHI GRCGYC 1 cut(s) 1402
BsaJI CCNNGG 3 cut(s) 172, 189, 371
BsaWI WCCGGW 1 cut(s) 217
Bsc4I CCNNNNNNNGG 1 cut(s) 1441
Bse118I RCCGGY 2 cut(s) 426, 463
Bse1I ACTGG 2 cut(s) 529, 1234
Bse21I CCTNAGG 1 cut(s) 1191
Bse3DI GCAATG 4 cut(s) 121, 404, 777, 1364
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 3 cut(s) 172, 189, 371
BseGI GGATG 3 cut(s) 278, 488, 1456
BseLI CCNNNNNNNGG 1 cut(s) 1441
BseMI GCAATG 4 cut(s) 121, 404, 777, 1364
BseMII CTCAG 1 cut(s) 1182
BseNI ACTGG 2 cut(s) 529, 1234
BseRI GAGGAG 2 cut(s) 1173, 1176
BseYI CCCAGC 1 cut(s) 757
BsgI GTGCAG 1 cut(s) 1284
BshFI GGCC 8 cut(s) 159, 202, 361, 370, 554, 1134, 1248, 1388
BshNI GGYRCC 4 cut(s) 214, 905, 962, 1401
BsiHKAI GWGCWC 2 cut(s) 1039, 1318
BsiSI CCGG 7 cut(s) 212, 218, 427, 464, 555, 706, 954
BslFI GGGAC 1 cut(s) 1418
BslI CCNNNNNNNGG 1 cut(s) 1441
BsmAI GTCTC 4 cut(s) 43, 675, 770, 1220
BsmFI GGGAC 1 cut(s) 1418
BsnI GGCC 8 cut(s) 159, 202, 361, 370, 554, 1134, 1248, 1388
Bsp1286I GDGCHC 2 cut(s) 1039, 1318
Bsp143I GATC 4 cut(s) 64, 598, 806, 1324
BspACI CCGC 1 cut(s) 177
BspANI GGCC 8 cut(s) 159, 202, 361, 370, 554, 1134, 1248, 1388
BspCNI CTCAG 1 cut(s) 1183
BspHI TCATGA 3 cut(s) 82, 1213, 1327
BspLI GGNNCC 7 cut(s) 216, 222, 468, 907, 964, 1387, 1403
BspMAI CTGCAG 1 cut(s) 717
BspT107I GGYRCC 4 cut(s) 214, 905, 962, 1401
BsrDI GCAATG 4 cut(s) 121, 404, 777, 1364
BsrFI RCCGGY 2 cut(s) 426, 463
BsrI ACTGG 2 cut(s) 529, 1234
BssAI RCCGGY 2 cut(s) 426, 463
BssECI CCNNGG 3 cut(s) 172, 189, 371
BssMI GATC 4 cut(s) 64, 598, 806, 1324
BssNI GRCGYC 1 cut(s) 1402
BssSI CACGAG 1 cut(s) 1032
BssT1I CCWWGG 2 cut(s) 189, 371
Bst2BI CACGAG 1 cut(s) 1032
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 1 cut(s) 1320
Bst6I CTCTTC 3 cut(s) 39, 41, 1267
BstACI GRCGYC 1 cut(s) 1402
BstAPI GCANNNNNTGC 1 cut(s) 120
BstC8I GCNNGC 1 cut(s) 316
BstDEI CTNAG 3 cut(s) 852, 1020, 1191
BstDSI CCRYGG 1 cut(s) 172
BstF5I GGATG 3 cut(s) 278, 488, 1456
BstH2I RGCGCY 1 cut(s) 1405
BstHHI GCGC 1 cut(s) 1404
BstKTI GATC 4 cut(s) 67, 601, 809, 1327
BstMAI GTCTC 4 cut(s) 43, 675, 770, 1220
BstMBI GATC 4 cut(s) 64, 598, 806, 1324
BstMWI GCNNNNNNNGC 9 cut(s) 120, 129, 367, 767, 1034, 1095, 1175, 1184, 1298
BstNI CCWGG 1 cut(s) 199
BstNSI RCATGY 1 cut(s) 1085
BstSCI CCNGG 3 cut(s) 197, 704, 953
BstSFI CTRYAG 2 cut(s) 713, 1182
BstV2I GAAGAC 6 cut(s) 21, 237, 429, 461, 675, 1356
Bsu36I CCTNAGG 1 cut(s) 1191
BsuRI GGCC 8 cut(s) 159, 202, 361, 370, 554, 1134, 1248, 1388
BtgI CCRYGG 1 cut(s) 172
BtsCI GGATG 3 cut(s) 278, 488, 1456
BtsI GCAGTG 1 cut(s) 103
BtsIMutI CAGTG 2 cut(s) 103, 1287
Cac8I GCNNGC 1 cut(s) 316
CaiI CAGNNNCTG 1 cut(s) 1190
CciI TCATGA 3 cut(s) 82, 1213, 1327
CfoI GCGC 1 cut(s) 1404
Cfr10I RCCGGY 2 cut(s) 426, 463
Cfr13I GGNCC 3 cut(s) 359, 368, 1386
DdeI CTNAG 3 cut(s) 852, 1020, 1191
DinI GGCGCC 1 cut(s) 1403
DpnI GATC 4 cut(s) 66, 600, 808, 1326
DpnII GATC 4 cut(s) 64, 598, 806, 1324
EaeI YGGCCR 3 cut(s) 157, 552, 1132
Eam1104I CTCTTC 3 cut(s) 39, 41, 1267
EarI CTCTTC 3 cut(s) 39, 41, 1267
EciI GGCGGA 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 189, 371
Eco147I AGGCCT 1 cut(s) 202
Eco32I GATATC 1 cut(s) 340
Eco57I CTGAAG 3 cut(s) 249, 1377, 1416
Eco81I CCTNAGG 1 cut(s) 1191
EcoRII CCWGG 1 cut(s) 197
EcoRV GATATC 1 cut(s) 340
EcoT14I CCWWGG 2 cut(s) 189, 371
EgeI GGCGCC 1 cut(s) 1403
EheI GGCGCC 1 cut(s) 1403
ErhI CCWWGG 2 cut(s) 189, 371
FalI AAGNNNNNCTT 4 cut(s) 414, 446, 556, 588
FaqI GGGAC 1 cut(s) 1418
FbaI TGATCA 2 cut(s) 598, 1324
FblI GTMKAC 1 cut(s) 1236
FokI GGATG 2 cut(s) 265, 475
FspBI CTAG 5 cut(s) 270, 519, 813, 1044, 1269
GlaI GCGC 1 cut(s) 1403
GsaI CCCAGC 1 cut(s) 761
HaeII RGCGCY 1 cut(s) 1405
HaeIII GGCC 8 cut(s) 159, 202, 361, 370, 554, 1134, 1248, 1388
HapII CCGG 7 cut(s) 212, 218, 427, 464, 555, 706, 954
HhaI GCGC 1 cut(s) 1404
Hin1I GRCGYC 1 cut(s) 1402
Hin6I GCGC 1 cut(s) 1402
HinP1I GCGC 1 cut(s) 1402
HindIII AAGCTT 2 cut(s) 130, 316
HinfI GANTC 7 cut(s) 718, 740, 836, 893, 1004, 1144, 1445
HpaII CCGG 7 cut(s) 212, 218, 427, 464, 555, 706, 954
HphI GGTGA 6 cut(s) 608, 677, 845, 902, 1013, 1235
Hpy166II GTNNAC 1 cut(s) 1237
Hpy188I TCNGA 7 cut(s) 229, 283, 351, 1041, 1199, 1324, 1450
Hpy188III TCNNGA 5 cut(s) 83, 1155, 1214, 1269, 1328
Hpy8I GTNNAC 1 cut(s) 1237
Hpy99I CGWCG 1 cut(s) 72
HpyAV CCTTC 6 cut(s) 80, 374, 495, 637, 763, 1391
HpyCH4III ACNGT 1 cut(s) 1320
HpyCH4IV ACGT 1 cut(s) 1106
HpyF10VI GCNNNNNNNGC 9 cut(s) 120, 129, 367, 767, 1034, 1095, 1175, 1184, 1298
HpyF3I CTNAG 3 cut(s) 852, 1020, 1191
HpySE526I ACGT 1 cut(s) 1106
Hsp92I GRCGYC 1 cut(s) 1402
HspAI GCGC 1 cut(s) 1402
KasI GGCGCC 1 cut(s) 1401
KpnI GGTACC 1 cut(s) 966
Ksp22I TGATCA 2 cut(s) 598, 1324
Kzo9I GATC 4 cut(s) 64, 598, 806, 1324
LmnI GCTCC 4 cut(s) 1042, 1183, 1297, 1454
LweI GCATC 1 cut(s) 146
MaeI CTAG 5 cut(s) 270, 519, 813, 1044, 1269
MaeII ACGT 1 cut(s) 1106
MalI GATC 4 cut(s) 66, 600, 808, 1326
MboI GATC 4 cut(s) 64, 598, 806, 1324
MhlI GDGCHC 2 cut(s) 1039, 1318
MlsI TGGCCA 1 cut(s) 1134
MluCI AATT 7 cut(s) 27, 345, 441, 504, 790, 1092, 1150
MluNI TGGCCA 1 cut(s) 1134
Mly113I GGCGCC 1 cut(s) 1402
MlyI GAGTC 1 cut(s) 749
MmeI TCCRAC 2 cut(s) 371, 1064
Mox20I TGGCCA 1 cut(s) 1134
MroXI GAANNNNTTC 3 cut(s) 131, 526, 571
MscI TGGCCA 1 cut(s) 1134
MseI TTAA 2 cut(s) 1110, 1462
MslI CAYNNNNRTG 3 cut(s) 1218, 1254, 1332
Msp20I TGGCCA 1 cut(s) 1134
MspA1I CMGCKG 1 cut(s) 786
MspI CCGG 7 cut(s) 212, 218, 427, 464, 555, 706, 954
MspR9I CCNGG 3 cut(s) 199, 706, 955
MvaI CCWGG 1 cut(s) 199
MwoI GCNNNNNNNGC 9 cut(s) 120, 129, 367, 767, 1034, 1095, 1175, 1184, 1298
NarI GGCGCC 1 cut(s) 1402
NciI CCSGG 2 cut(s) 706, 955
NdeII GATC 4 cut(s) 64, 598, 806, 1324
NlaIV GGNNCC 7 cut(s) 216, 222, 468, 907, 964, 1387, 1403
NspI RCATGY 1 cut(s) 1085
OliI CACNNNNGTG 1 cut(s) 1254
PagI TCATGA 3 cut(s) 82, 1213, 1327
PceI AGGCCT 1 cut(s) 202
PcsI WCGNNNNNNNCGW 1 cut(s) 815
PdmI GAANNNNTTC 3 cut(s) 131, 526, 571
PfeI GAWTC 6 cut(s) 718, 836, 893, 1004, 1144, 1445
PleI GAGTC 1 cut(s) 748
PluTI GGCGCC 1 cut(s) 1405
PpsI GAGTC 1 cut(s) 748
Psp6I CCWGG 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 757
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 7 cut(s) 216, 222, 468, 907, 964, 1387, 1403
PspPI GGNCC 3 cut(s) 359, 368, 1386
PstI CTGCAG 1 cut(s) 717
PstNI CAGNNNCTG 1 cut(s) 1190
PvuII CAGCTG 1 cut(s) 786
RseI CAYNNNNRTG 3 cut(s) 1218, 1254, 1332
SaqAI TTAA 2 cut(s) 1110, 1462
Sau3AI GATC 4 cut(s) 64, 598, 806, 1324
Sau96I GGNCC 3 cut(s) 359, 368, 1386
ScaI AGTACT 2 cut(s) 982, 1048
SchI GAGTC 1 cut(s) 749
ScrFI CCNGG 3 cut(s) 199, 706, 955
SduI GDGCHC 2 cut(s) 1039, 1318
SfaNI GCATC 1 cut(s) 146
SfcI CTRYAG 2 cut(s) 713, 1182
SfiI GGCCNNNNNGGCC 1 cut(s) 367
SfoI GGCGCC 1 cut(s) 1403
SmiMI CAYNNNNRTG 3 cut(s) 1218, 1254, 1332
SmlI CTYRAG 3 cut(s) 143, 331, 1242
SmoI CTYRAG 3 cut(s) 143, 331, 1242
SpeI ACTAGT 1 cut(s) 1043
Sse9I AATT 7 cut(s) 27, 345, 441, 504, 790, 1092, 1150
SseBI AGGCCT 1 cut(s) 202
SsiI CCGC 1 cut(s) 177
SspDI GGCGCC 1 cut(s) 1401
SspI AATATT 1 cut(s) 634
SspMI CTAG 5 cut(s) 270, 519, 813, 1044, 1269
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 3 cut(s) 197, 704, 953
StyI CCWWGG 2 cut(s) 189, 371
TaaI ACNGT 1 cut(s) 1320
TaiI ACGT 1 cut(s) 1109
TaqI TCGA 2 cut(s) 342, 1154
TasI AATT 7 cut(s) 27, 345, 441, 504, 790, 1092, 1150
TatI WGTACW 2 cut(s) 980, 1046
TfiI GAWTC 6 cut(s) 718, 836, 893, 1004, 1144, 1445
Tru1I TTAA 2 cut(s) 1110, 1462
Tru9I TTAA 2 cut(s) 1110, 1462
TscAI CASTG 2 cut(s) 103, 1287
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 2 cut(s) 103, 1287
XapI RAATTY 3 cut(s) 345, 441, 1092
XbaI TCTAGA 1 cut(s) 1268
XceI RCATGY 1 cut(s) 1085
XcmI CCANNNNNNNNNTGG 2 cut(s) 682, 1256
XmiI GTMKAC 1 cut(s) 1236
XmnI GAANNNNTTC 3 cut(s) 131, 526, 571
XspI CTAG 5 cut(s) 270, 519, 813, 1044, 1269
ZrmI AGTACT 2 cut(s) 982, 1048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.