Rroxscaffold_1G00027610

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0023090
Physical Location & Seq
Reverse (-)
35003711 .. 35004073
363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00027610.1

Sequence Viewer

Length: 243 bp
ATGGAAGAAAAACACCTGGCATGTTCGAAAAGATTCATCCAAAGCAATGGCCATGAGCATGCAGTTGGGTATGTGAGTGGAGATCAATACTATGGAGCAAAAGCAAGTATCAACGTCCGGGCACCAAAGGCTGCAGCTAGGACGCTCTTCTCGAAGAGGTTTGGTCACCGTGTTTGCAACAAAGAGCATGCCCATGGCTGGACAGAAAACCGTGAGGTTCCAACTATGGACTCGATTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

80

Amino Acids

9.07

Weight (kDa)

9.56

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0023090)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002833.1_g000021
rosa_roxburghii Rroxscaffold_1G00027610 Rroxscaffold_1G00028710 Rroxscaffold_6G00394080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 121
AcoI YGGCCR 1 cut(s) 49
AfiI CCNNNNNNNGG 1 cut(s) 198
AjnI CCWGG 1 cut(s) 15
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
AoxI GGCC 1 cut(s) 49
ApeKI GCWGC 2 cut(s) 131, 134
Asp700I GAANNNNTTC 1 cut(s) 32
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 1 cut(s) 158
AsuII TTCGAA 1 cut(s) 26
BaeGI GKGCMC 1 cut(s) 124
BalI TGGCCA 1 cut(s) 51
BanI GGYRCC 1 cut(s) 121
BbvI GCAGC 2 cut(s) 118, 146
BciT130I CCWGG 1 cut(s) 17
BcnI CCSGG 1 cut(s) 119
BfaI CTAG 1 cut(s) 138
BfmI CTRYAG 1 cut(s) 132
BisI GCNGC 2 cut(s) 132, 135
BlsI GCNGC 2 cut(s) 133, 136
Bme1390I CCNGG 2 cut(s) 17, 119
BmiI GGNNCC 2 cut(s) 123, 219
BmrFI CCNGG 2 cut(s) 17, 119
Bpu14I TTCGAA 1 cut(s) 26
BpuMI CCSGG 1 cut(s) 119
BsaJI CCNNGG 1 cut(s) 193
Bsc4I CCNNNNNNNGG 1 cut(s) 198
Bse3DI GCAATG 1 cut(s) 52
BseBI CCWGG 1 cut(s) 17
BseDI CCNNGG 1 cut(s) 193
BseGI GGATG 1 cut(s) 36
BseLI CCNNNNNNNGG 1 cut(s) 198
BseMI GCAATG 1 cut(s) 52
BseSI GKGCMC 1 cut(s) 124
BseXI GCAGC 2 cut(s) 118, 146
BshFI GGCC 1 cut(s) 51
BshNI GGYRCC 1 cut(s) 121
BsiSI CCGG 1 cut(s) 118
BslI CCNNNNNNNGG 1 cut(s) 198
BsnI GGCC 1 cut(s) 51
Bsp119I TTCGAA 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 124
Bsp143I GATC 1 cut(s) 82
Bsp19I CCATGG 1 cut(s) 193
BspANI GGCC 1 cut(s) 51
BspLI GGNNCC 2 cut(s) 123, 219
BspMAI CTGCAG 1 cut(s) 136
BspQI GCTCTTC 1 cut(s) 152
BspT104I TTCGAA 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 52
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 1 cut(s) 82
BssT1I CCWWGG 1 cut(s) 193
Bst2UI CCWGG 1 cut(s) 17
Bst4CI ACNGT 2 cut(s) 170, 212
Bst6I CTCTTC 2 cut(s) 149, 152
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 60, 189
BstDSI CCRYGG 1 cut(s) 193
BstEII GGTNACC 1 cut(s) 164
BstF5I GGATG 1 cut(s) 36
BstKTI GATC 1 cut(s) 85
BstMBI GATC 1 cut(s) 82
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstNI CCWGG 1 cut(s) 17
BstNSI RCATGY 3 cut(s) 24, 62, 191
BstPI GGTNACC 1 cut(s) 164
BstSCI CCNGG 2 cut(s) 15, 117
BstSFI CTRYAG 1 cut(s) 132
BstSLI GKGCMC 1 cut(s) 124
BstV1I GCAGC 2 cut(s) 118, 146
BstXI CCANNNNNNTGG 1 cut(s) 47
BsuRI GGCC 1 cut(s) 51
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 1 cut(s) 36
Cac8I GCNNGC 2 cut(s) 60, 189
CseI GACGC 1 cut(s) 151
CviAII CATG 5 cut(s) 21, 53, 59, 188, 194
CviJI RGCY 4 cut(s) 51, 131, 137, 198
CviKI_1 RGCY 4 cut(s) 51, 131, 137, 198
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
EaeI YGGCCR 1 cut(s) 49
Eam1104I CTCTTC 2 cut(s) 149, 152
EarI CTCTTC 2 cut(s) 149, 152
Eco130I CCWWGG 1 cut(s) 193
Eco91I GGTNACC 1 cut(s) 164
EcoO65I GGTNACC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 15
EcoT14I CCWWGG 1 cut(s) 193
ErhI CCWWGG 1 cut(s) 193
FaeI CATG 5 cut(s) 24, 56, 62, 191, 197
FaiI YATR 8 cut(s) 22, 54, 60, 72, 93, 189, 195, 227
FatI CATG 5 cut(s) 20, 52, 58, 187, 193
Fnu4HI GCNGC 2 cut(s) 132, 135
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 2 cut(s) 132, 135
FspBI CTAG 1 cut(s) 138
GluI GCNGC 2 cut(s) 132, 135
HaeIII GGCC 1 cut(s) 51
HapII CCGG 1 cut(s) 118
HgaI GACGC 1 cut(s) 151
Hin1II CATG 5 cut(s) 24, 56, 62, 191, 197
HinfI GANTC 3 cut(s) 33, 230, 235
HpaII CCGG 1 cut(s) 118
HphI GGTGA 1 cut(s) 158
Hpy188III TCNNGA 1 cut(s) 151
HpyCH4III ACNGT 2 cut(s) 170, 212
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 3 cut(s) 62, 134, 177
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 5 cut(s) 24, 56, 62, 191, 197
Kzo9I GATC 1 cut(s) 82
LguI GCTCTTC 1 cut(s) 152
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 4 cut(s) 2, 29, 131, 184
Lsp1109I GCAGC 2 cut(s) 118, 146
MaeI CTAG 1 cut(s) 138
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 1 cut(s) 164
MalI GATC 1 cut(s) 84
MboI GATC 1 cut(s) 82
MboII GAAGA 3 cut(s) 17, 139, 166
MhlI GDGCHC 1 cut(s) 124
MlsI TGGCCA 1 cut(s) 51
MluNI TGGCCA 1 cut(s) 51
MlyI GAGTC 1 cut(s) 224
MnlI CCTC 2 cut(s) 150, 208
Mox20I TGGCCA 1 cut(s) 51
MroXI GAANNNNTTC 1 cut(s) 32
MscI TGGCCA 1 cut(s) 51
MslI CAYNNNNRTG 2 cut(s) 57, 192
Msp20I TGGCCA 1 cut(s) 51
MspI CCGG 1 cut(s) 118
MspR9I CCNGG 2 cut(s) 17, 119
MvaI CCWGG 1 cut(s) 17
MwoI GCNNNNNNNGC 1 cut(s) 128
NciI CCSGG 1 cut(s) 119
NcoI CCATGG 1 cut(s) 193
NdeII GATC 1 cut(s) 82
NlaIII CATG 5 cut(s) 24, 56, 62, 191, 197
NlaIV GGNNCC 2 cut(s) 123, 219
NmuCI GTSAC 1 cut(s) 164
NspI RCATGY 3 cut(s) 24, 62, 191
NspV TTCGAA 1 cut(s) 26
PaeI GCATGC 2 cut(s) 62, 191
PciSI GCTCTTC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 1 cut(s) 149
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 2 cut(s) 33, 235
PkrI GCNGC 2 cut(s) 133, 136
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 15
PspEI GGTNACC 1 cut(s) 164
PspGI CCWGG 1 cut(s) 15
PspN4I GGNNCC 2 cut(s) 123, 219
PstI CTGCAG 1 cut(s) 136
RseI CAYNNNNRTG 2 cut(s) 57, 192
SapI GCTCTTC 1 cut(s) 152
SatI GCNGC 2 cut(s) 132, 135
Sau3AI GATC 1 cut(s) 82
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 2 cut(s) 17, 119
SduI GDGCHC 1 cut(s) 124
SetI ASST 5 cut(s) 18, 117, 139, 161, 219
SfcI CTRYAG 1 cut(s) 132
SfuI TTCGAA 1 cut(s) 26
SmiMI CAYNNNNRTG 2 cut(s) 57, 192
SphI GCATGC 2 cut(s) 62, 191
SspMI CTAG 1 cut(s) 138
StyD4I CCNGG 2 cut(s) 15, 117
StyI CCWWGG 1 cut(s) 193
TaaI ACNGT 2 cut(s) 170, 212
TaiI ACGT 1 cut(s) 117
TaqI TCGA 3 cut(s) 26, 152, 233
TfiI GAWTC 2 cut(s) 33, 235
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 2 cut(s) 131, 134
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 1 cut(s) 25
XceI RCATGY 3 cut(s) 24, 62, 191
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.