Rroxscaffold_1G00028710

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0023090
Physical Location & Seq
Forward (+)
36695709 .. 36696165
457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00028710.1

Sequence Viewer

Length: 393 bp
ATGGAGCTTGTCGAGTATTATATGCCCAGATGGTACAATTCCGGTTCGAAGAACAAGAGAGCAAGACATGCTCGAGCTAGCTCGGTTCGAAGATATGGAAGAAAAACACCCAGGCATGTTCGAAAAGATTCATCCAGCAATGGCCATGAGCATGCAGTTGGGTATGTGAGTGGAGATCAATACTATGGAGCAAAAGCAAGTATCAACGTCCGGGCACCAAAGGTAGATGGGGAGGTTGAGTTCCCATTTCCAGCGGGGAAATACAGCTTGTATTTTAGGCTGCAGCTAGGACGCTCTTCTCGAAGAGGTTTGGTCACCGTGTTTGCAACAAAGAGCATGCCCATGGCTGGACAGAAAACCGGTGAGGTTCCAACTATGGACTCGATTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

130

Amino Acids

14.72

Weight (kDa)

10.37

Isoelectric Point (pI)

55.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023090)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002833.1_g000021
rosa_roxburghii Rroxscaffold_1G00027610 Rroxscaffold_1G00028710 Rroxscaffold_6G00394080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 214
AciI CCGC 1 cut(s) 254
AcoI YGGCCR 1 cut(s) 142
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 1 cut(s) 347
AgeI ACCGGT 1 cut(s) 359
AjnI CCWGG 1 cut(s) 110
AloI GAACNNNNNNTCC 2 cut(s) 224, 256
AluBI AGCT 5 cut(s) 7, 77, 81, 267, 286
AluI AGCT 5 cut(s) 7, 77, 81, 267, 286
Ama87I CYCGRG 1 cut(s) 72
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 2 cut(s) 280, 283
AsiGI ACCGGT 1 cut(s) 359
Asp700I GAANNNNTTC 1 cut(s) 127
AsuC2I CCSGG 1 cut(s) 212
AsuHPI GGTGA 2 cut(s) 307, 374
AsuII TTCGAA 3 cut(s) 47, 88, 121
AsuNHI GCTAGC 1 cut(s) 77
AvaI CYCGRG 1 cut(s) 72
BaeGI GKGCMC 1 cut(s) 217
BalI TGGCCA 1 cut(s) 144
BanI GGYRCC 1 cut(s) 214
BbvI GCAGC 2 cut(s) 267, 295
BccI CCATC 2 cut(s) 24, 221
BciT130I CCWGG 1 cut(s) 112
BcnI CCSGG 1 cut(s) 212
BfaI CTAG 2 cut(s) 78, 287
BfmI CTRYAG 1 cut(s) 281
BisI GCNGC 2 cut(s) 281, 284
BlsI GCNGC 2 cut(s) 282, 285
Bme1390I CCNGG 2 cut(s) 112, 212
BmeT110I CYCGRG 1 cut(s) 72
BmiI GGNNCC 2 cut(s) 216, 369
BmrFI CCNGG 2 cut(s) 112, 212
BmtI GCTAGC 1 cut(s) 81
Bpu14I TTCGAA 3 cut(s) 47, 88, 121
BpuMI CCSGG 1 cut(s) 212
BsaJI CCNNGG 2 cut(s) 110, 342
BsaWI WCCGGW 2 cut(s) 41, 359
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 1 cut(s) 347
Bse118I RCCGGY 1 cut(s) 359
Bse3DI GCAATG 1 cut(s) 145
BseBI CCWGG 1 cut(s) 112
BseDI CCNNGG 2 cut(s) 110, 342
BseGI GGATG 1 cut(s) 131
BseLI CCNNNNNNNGG 1 cut(s) 347
BseMI GCAATG 1 cut(s) 145
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 2 cut(s) 267, 295
BshFI GGCC 1 cut(s) 144
BshNI GGYRCC 1 cut(s) 214
BshTI ACCGGT 1 cut(s) 359
BsiHKCI CYCGRG 1 cut(s) 72
BsiSI CCGG 3 cut(s) 42, 211, 360
BslI CCNNNNNNNGG 1 cut(s) 347
BsnI GGCC 1 cut(s) 144
BsoBI CYCGRG 1 cut(s) 72
Bsp119I TTCGAA 3 cut(s) 47, 88, 121
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 1 cut(s) 175
Bsp19I CCATGG 1 cut(s) 342
BspACI CCGC 1 cut(s) 254
BspANI GGCC 1 cut(s) 144
BspLI GGNNCC 2 cut(s) 216, 369
BspMAI CTGCAG 1 cut(s) 285
BspOI GCTAGC 1 cut(s) 81
BspQI GCTCTTC 1 cut(s) 301
BspT104I TTCGAA 3 cut(s) 47, 88, 121
BspT107I GGYRCC 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 145
BsrFI RCCGGY 1 cut(s) 359
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 2 cut(s) 110, 342
BssMI GATC 1 cut(s) 175
BssT1I CCWWGG 1 cut(s) 342
Bst2UI CCWGG 1 cut(s) 112
Bst4CI ACNGT 1 cut(s) 319
Bst6I CTCTTC 2 cut(s) 298, 301
BstAPI GCANNNNNTGC 1 cut(s) 68
BstBI TTCGAA 3 cut(s) 47, 88, 121
BstC8I GCNNGC 3 cut(s) 79, 153, 338
BstDSI CCRYGG 1 cut(s) 342
BstEII GGTNACC 1 cut(s) 313
BstF5I GGATG 1 cut(s) 131
BstKTI GATC 1 cut(s) 178
BstMBI GATC 1 cut(s) 175
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 1 cut(s) 112
BstNSI RCATGY 4 cut(s) 71, 119, 155, 340
BstPI GGTNACC 1 cut(s) 313
BstSCI CCNGG 2 cut(s) 110, 210
BstSFI CTRYAG 1 cut(s) 281
BstSLI GKGCMC 1 cut(s) 217
BstV1I GCAGC 2 cut(s) 267, 295
BsuRI GGCC 1 cut(s) 144
BtgI CCRYGG 1 cut(s) 342
BtsCI GGATG 1 cut(s) 131
Cac8I GCNNGC 3 cut(s) 79, 153, 338
Cfr10I RCCGGY 1 cut(s) 359
CseI GACGC 1 cut(s) 300
Csp6I GTAC 1 cut(s) 34
CspAI ACCGGT 1 cut(s) 359
CviAII CATG 6 cut(s) 68, 116, 146, 152, 337, 343
CviJI RGCY 8 cut(s) 7, 77, 81, 144, 267, 280, 286, 347
CviKI_1 RGCY 8 cut(s) 7, 77, 81, 144, 267, 280, 286, 347
CviQI GTAC 1 cut(s) 34
DpnI GATC 1 cut(s) 177
DpnII GATC 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 2 cut(s) 298, 301
EarI CTCTTC 2 cut(s) 298, 301
Eco130I CCWWGG 1 cut(s) 342
Eco88I CYCGRG 1 cut(s) 72
Eco91I GGTNACC 1 cut(s) 313
EcoO65I GGTNACC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 110
EcoT14I CCWWGG 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 6 cut(s) 71, 119, 149, 155, 340, 346
FatI CATG 6 cut(s) 67, 115, 145, 151, 336, 342
FauI CCCGC 1 cut(s) 247
Fnu4HI GCNGC 2 cut(s) 281, 284
FokI GGATG 1 cut(s) 118
Fsp4HI GCNGC 2 cut(s) 281, 284
FspBI CTAG 2 cut(s) 78, 287
GluI GCNGC 2 cut(s) 281, 284
HaeIII GGCC 1 cut(s) 144
HapII CCGG 3 cut(s) 42, 211, 360
HgaI GACGC 1 cut(s) 300
Hin1II CATG 6 cut(s) 71, 119, 149, 155, 340, 346
HinfI GANTC 3 cut(s) 128, 380, 385
HpaII CCGG 3 cut(s) 42, 211, 360
HphI GGTGA 2 cut(s) 307, 374
Hpy188III TCNNGA 1 cut(s) 300
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 3 cut(s) 155, 283, 326
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpySE526I ACGT 1 cut(s) 207
Hsp92II CATG 6 cut(s) 71, 119, 149, 155, 340, 346
Kzo9I GATC 1 cut(s) 175
LguI GCTCTTC 1 cut(s) 301
LmnI GCTCC 2 cut(s) 4, 188
LpnPI CCDG 9 cut(s) 40, 55, 97, 124, 148, 224, 264, 333, 373
Lsp1109I GCAGC 2 cut(s) 267, 295
MaeI CTAG 2 cut(s) 78, 287
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 1 cut(s) 313
MalI GATC 1 cut(s) 177
MboI GATC 1 cut(s) 175
MboII GAAGA 5 cut(s) 61, 102, 111, 288, 315
MhlI GDGCHC 1 cut(s) 217
MlsI TGGCCA 1 cut(s) 144
MluCI AATT 1 cut(s) 37
MluNI TGGCCA 1 cut(s) 144
MlyI GAGTC 1 cut(s) 374
MnlI CCTC 3 cut(s) 226, 299, 358
Mox20I TGGCCA 1 cut(s) 144
MroXI GAANNNNTTC 1 cut(s) 127
MscI TGGCCA 1 cut(s) 144
MslI CAYNNNNRTG 2 cut(s) 150, 341
Msp20I TGGCCA 1 cut(s) 144
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 3 cut(s) 42, 211, 360
MspR9I CCNGG 2 cut(s) 112, 212
MvaI CCWGG 1 cut(s) 112
MwoI GCNNNNNNNGC 1 cut(s) 68
NciI CCSGG 1 cut(s) 212
NcoI CCATGG 1 cut(s) 342
NdeII GATC 1 cut(s) 175
NheI GCTAGC 1 cut(s) 77
NlaIII CATG 6 cut(s) 71, 119, 149, 155, 340, 346
NlaIV GGNNCC 2 cut(s) 216, 369
NmuCI GTSAC 1 cut(s) 313
NspI RCATGY 4 cut(s) 71, 119, 155, 340
NspV TTCGAA 3 cut(s) 47, 88, 121
PaeI GCATGC 2 cut(s) 155, 340
PaeR7I CTCGAG 1 cut(s) 72
PciSI GCTCTTC 1 cut(s) 301
PcsI WCGNNNNNNNCGW 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 127
PfeI GAWTC 2 cut(s) 128, 385
PinAI ACCGGT 1 cut(s) 359
PkrI GCNGC 2 cut(s) 282, 285
PleI GAGTC 1 cut(s) 374
PpsI GAGTC 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 110
PspEI GGTNACC 1 cut(s) 313
PspGI CCWGG 1 cut(s) 110
PspN4I GGNNCC 2 cut(s) 216, 369
PspXI VCTCGAGB 1 cut(s) 72
PstI CTGCAG 1 cut(s) 285
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
RseI CAYNNNNRTG 2 cut(s) 150, 341
SapI GCTCTTC 1 cut(s) 301
SatI GCNGC 2 cut(s) 281, 284
Sau3AI GATC 1 cut(s) 175
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 2 cut(s) 112, 212
SduI GDGCHC 1 cut(s) 217
SfcI CTRYAG 1 cut(s) 281
Sfr274I CTCGAG 1 cut(s) 72
SfuI TTCGAA 3 cut(s) 47, 88, 121
SlaI CTCGAG 1 cut(s) 72
SmiMI CAYNNNNRTG 2 cut(s) 150, 341
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
SphI GCATGC 2 cut(s) 155, 340
Sse9I AATT 1 cut(s) 37
SsiI CCGC 1 cut(s) 254
SspMI CTAG 2 cut(s) 78, 287
StyD4I CCNGG 2 cut(s) 110, 210
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 1 cut(s) 210
TaqI TCGA 7 cut(s) 12, 47, 73, 88, 121, 301, 383
TasI AATT 1 cut(s) 37
TfiI GAWTC 2 cut(s) 128, 385
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 2 cut(s) 280, 283
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 1 cut(s) 120
XceI RCATGY 4 cut(s) 71, 119, 155, 340
XhoI CTCGAG 1 cut(s) 72
XmnI GAANNNNTTC 1 cut(s) 127
XspI CTAG 2 cut(s) 78, 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.