Rroxscaffold_1G00045520

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0021445
Physical Location & Seq
Forward (+)
64491004 .. 64504380
13377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00045520.1

Sequence Viewer

Length: 1125 bp
ATGCATGACCAACCCAAAGTGAAAACTCACAAAGTGCGAGGCTCGTCCGAGCATAGGGACAAGAGCAGCACCGATACCGGTAATCCTAATGGGCGTCACCCTAAACCCAACCGAACTAGCAGGATTAGGACCGACACCACCGCCACGGTTGCACAAGAGAAGATGGAGGGACCGCCACATCCGGATGTCGGGATCTTGACAAAACAATGGCGCCGGCACCTTGCGAGGACTCCTCAAGGCAATCCTATATGTCTAAGTCGAATACGGCACTTATGGCGCCACAACACCACCACCACAAACTCCACTCAAACCCCCAACCTCGCCACATTCCAACCAGGAAACAACACCACCACTCCAAACATAACCACACCGCCCAATGACAAAAAAGGGAGTGGCAGTAGGGTAACTACCAAGACAAGTGGCAAAAGGACCAACATTATCACAATAAGGGCAACAGATCTTTTTCACAATGATCGCTACGACAATCGCCACAATAAGGCCGCTACAAATGCGCAAAAGTTTGAGGTTTTCACAGTCTTGACGACGTCATATGAAGAAATATATGATCGGTGTAAGGATCAAATCCCACCACCACCCCCAAGAAAATACCCCGGGGTAGGAAAATCTAGAAACACCGTGAGGAAGAGGCTGACCCTCTTCCTTGTTTTCAATGTGTGGACCGATATGCGTCGCGGTCCAAGTTTGTGGAGCTTCGATGCTCTTTTGATGTTGCGCGTGGCGGTGCGTAAGCGGGTGATCCGGGGTCGGTTTTGCTTTGCTAGGCTACCCAGGTTCTCAAGACCACCTAGGGAACCAAAATATAGTCATTTGAAATATCTGCATAAAGCCATCAAATTAGTTGAGCCAGCTTTAGTGGCCACTGATGAGCTAATAACATTTATCTATGATAGTCCCCAGCTTCCTCTCTCTCCCCGATCCCCTCTCTGTTCCTCGCAAGAAAGAAAAAAGTCAAAATCGATGAAGCTTCAAATCCGATTCAACCCATCTCATCTCCGCTCTTTGCTTTCCGATCTACAACCGTATTGTACCCACCGCCAATTCCAGTTCCAAGCGCCGACATTAGTATCTTCTCCATCTAAATCTCACCCGTCAAGGCGTCGGTAA

Protein Analysis

374

Amino Acids

43.01

Weight (kDa)

11.03

Isoelectric Point (pI)

56.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021445)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0019801
rosa_roxburghii Rroxscaffold_1G00045520 Rroxscaffold_4G00326140 Rroxscaffold_4G00326150 Rroxscaffold_5G00364970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 548
Acc16I TGCGCA 1 cut(s) 513
AccB1I GGYRCC 3 cut(s) 210, 216, 276
AccBSI CCGCTC 1 cut(s) 1017
AccII CGCG 2 cut(s) 693, 735
AccIII TCCGGA 1 cut(s) 181
AciI CCGC 9 cut(s) 141, 173, 371, 501, 693, 740, 751, 1015, 1054
AclWI GGATC 4 cut(s) 200, 585, 751, 930
AcoI YGGCCR 1 cut(s) 876
AcyI GRCGYC 5 cut(s) 94, 211, 277, 545, 1117
AdeI CACNNNGTG 1 cut(s) 34
AfaI GTAC 1 cut(s) 1048
AfiI CCNNNNNNNGG 4 cut(s) 54, 188, 496, 617
AgeI ACCGGT 1 cut(s) 77
AgsI TTSAA 4 cut(s) 670, 832, 989, 1000
AjnI CCWGG 2 cut(s) 334, 788
AluBI AGCT 5 cut(s) 711, 869, 889, 919, 985
AluI AGCT 5 cut(s) 711, 869, 889, 919, 985
AlwI GGATC 4 cut(s) 200, 585, 751, 930
Ama87I CYCGRG 1 cut(s) 611
Aor13HI TCCGGA 1 cut(s) 181
AoxI GGCC 2 cut(s) 498, 876
ApeKI GCWGC 1 cut(s) 66
AsiGI ACCGGT 1 cut(s) 77
AspA2I CCTAGG 1 cut(s) 806
AspLEI GCGC 5 cut(s) 213, 279, 514, 735, 1075
AspS9I GGNCC 5 cut(s) 129, 170, 429, 678, 695
AsuC2I CCSGG 3 cut(s) 612, 613, 761
AsuHPI GGTGA 3 cut(s) 89, 766, 1097
AvaI CYCGRG 1 cut(s) 611
AvaII GGWCC 5 cut(s) 129, 170, 429, 678, 695
AvrII CCTAGG 1 cut(s) 806
BaeI ACNNNNGTAYC 2 cut(s) 1068, 1101
BalI TGGCCA 1 cut(s) 878
BanI GGYRCC 3 cut(s) 210, 216, 276
BbvI GCAGC 1 cut(s) 78
BccI CCATC 4 cut(s) 157, 857, 1012, 1102
BceAI ACGGC 1 cut(s) 281
BciT130I CCWGG 2 cut(s) 336, 790
BcnI CCSGG 3 cut(s) 612, 613, 761
BfaI CTAG 4 cut(s) 117, 627, 780, 807
BfoI RGCGCY 3 cut(s) 214, 280, 1076
BglII AGATCT 1 cut(s) 457
BisI GCNGC 2 cut(s) 67, 501
BlnI CCTAGG 1 cut(s) 806
BlsI GCNGC 2 cut(s) 68, 502
Bme1390I CCNGG 5 cut(s) 336, 612, 613, 761, 790
Bme18I GGWCC 5 cut(s) 129, 170, 429, 678, 695
BmeT110I CYCGRG 1 cut(s) 611
BmgT120I GGNCC 5 cut(s) 129, 170, 429, 678, 695
BmiI GGNNCC 5 cut(s) 171, 212, 218, 278, 813
BmrFI CCNGG 5 cut(s) 336, 612, 613, 761, 790
BmsI GCATC 1 cut(s) 706
BplI GAGNNNNNCTC 2 cut(s) 217, 249
BpuEI CTTGAG 2 cut(s) 219, 781
BpuMI CCSGG 3 cut(s) 612, 613, 761
Bsa29I ATCGAT 1 cut(s) 977
BsaHI GRCGYC 5 cut(s) 94, 211, 277, 545, 1117
BsaJI CCNNGG 7 cut(s) 144, 610, 611, 612, 760, 788, 806
BsaWI WCCGGW 2 cut(s) 77, 181
BsaXI ACNNNNNCTCC 4 cut(s) 337, 367, 382, 412
Bsc4I CCNNNNNNNGG 4 cut(s) 54, 188, 496, 617
Bse118I RCCGGY 2 cut(s) 77, 213
Bse1I ACTGG 1 cut(s) 1063
BseAI TCCGGA 1 cut(s) 181
BseBI CCWGG 2 cut(s) 336, 790
BseCI ATCGAT 1 cut(s) 977
BseDI CCNNGG 7 cut(s) 144, 610, 611, 612, 760, 788, 806
BseGI GGATG 2 cut(s) 178, 190
BseLI CCNNNNNNNGG 4 cut(s) 54, 188, 496, 617
BseNI ACTGG 1 cut(s) 1063
BseRI GAGGAG 1 cut(s) 222
BseXI GCAGC 1 cut(s) 78
BseYI CCCAGC 1 cut(s) 915
Bsh1236I CGCG 2 cut(s) 693, 735
BshFI GGCC 2 cut(s) 500, 878
BshNI GGYRCC 3 cut(s) 210, 216, 276
BshTI ACCGGT 1 cut(s) 77
BshVI ATCGAT 1 cut(s) 977
BsiHKCI CYCGRG 1 cut(s) 611
BsiSI CCGG 5 cut(s) 78, 182, 214, 612, 760
BslFI GGGAC 3 cut(s) 71, 183, 897
BslI CCNNNNNNNGG 4 cut(s) 54, 188, 496, 617
BsmFI GGGAC 3 cut(s) 71, 183, 897
BsnI GGCC 2 cut(s) 500, 878
BsoBI CYCGRG 1 cut(s) 611
Bsp13I TCCGGA 1 cut(s) 181
Bsp143I GATC 8 cut(s) 192, 457, 472, 565, 577, 756, 935, 1030
BspACI CCGC 9 cut(s) 141, 173, 371, 501, 693, 740, 751, 1015, 1054
BspANI GGCC 2 cut(s) 500, 878
BspDI ATCGAT 1 cut(s) 977
BspEI TCCGGA 1 cut(s) 181
BspFNI CGCG 2 cut(s) 693, 735
BspLI GGNNCC 5 cut(s) 171, 212, 218, 278, 813
BspPI GGATC 4 cut(s) 200, 585, 751, 930
BspT107I GGYRCC 3 cut(s) 210, 216, 276
BsrBI CCGCTC 1 cut(s) 1017
BsrFI RCCGGY 2 cut(s) 77, 213
BsrI ACTGG 1 cut(s) 1063
BssAI RCCGGY 2 cut(s) 77, 213
BssECI CCNNGG 7 cut(s) 144, 610, 611, 612, 760, 788, 806
BssMI GATC 8 cut(s) 192, 457, 472, 565, 577, 756, 935, 1030
BssNI GRCGYC 5 cut(s) 94, 211, 277, 545, 1117
BssT1I CCWWGG 1 cut(s) 806
Bst2UI CCWGG 2 cut(s) 336, 790
Bst4CI ACNGT 4 cut(s) 148, 535, 637, 1041
Bst6I CTCTTC 2 cut(s) 638, 662
BstACI GRCGYC 5 cut(s) 94, 211, 277, 545, 1117
BstC8I GCNNGC 2 cut(s) 215, 867
BstDEI CTNAG 1 cut(s) 254
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 2 cut(s) 178, 190
BstFNI CGCG 2 cut(s) 693, 735
BstH2I RGCGCY 3 cut(s) 214, 280, 1076
BstHHI GCGC 5 cut(s) 213, 279, 514, 735, 1075
BstKTI GATC 8 cut(s) 195, 460, 475, 568, 580, 759, 938, 1033
BstMBI GATC 8 cut(s) 192, 457, 472, 565, 577, 756, 935, 1030
BstMWI GCNNNNNNNGC 4 cut(s) 149, 274, 509, 875
BstNI CCWGG 2 cut(s) 336, 790
BstSCI CCNGG 5 cut(s) 334, 610, 611, 759, 788
BstUI CGCG 2 cut(s) 693, 735
BstV1I GCAGC 1 cut(s) 78
BstX2I RGATCY 2 cut(s) 192, 457
BstXI CCANNNNNNTGG 1 cut(s) 705
BstYI RGATCY 2 cut(s) 192, 457
Bsu15I ATCGAT 1 cut(s) 977
BsuRI GGCC 2 cut(s) 500, 878
BsuTUI ATCGAT 1 cut(s) 977
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 2 cut(s) 178, 190
BtsIMutI CAGTG 1 cut(s) 879
Cac8I GCNNGC 2 cut(s) 215, 867
CfoI GCGC 5 cut(s) 213, 279, 514, 735, 1075
Cfr10I RCCGGY 2 cut(s) 77, 213
Cfr13I GGNCC 5 cut(s) 129, 170, 429, 678, 695
Cfr9I CCCGGG 1 cut(s) 611
ClaI ATCGAT 1 cut(s) 977
CseI GACGC 3 cut(s) 83, 677, 1106
Csp6I GTAC 1 cut(s) 1047
CspAI ACCGGT 1 cut(s) 77
CspCI CAANNNNNGTGG 2 cut(s) 400, 435
CviAII CATG 1 cut(s) 5
CviQI GTAC 1 cut(s) 1047
DdeI CTNAG 1 cut(s) 254
DinI GGCGCC 2 cut(s) 212, 278
DpnI GATC 8 cut(s) 194, 459, 474, 567, 579, 758, 937, 1032
DpnII GATC 8 cut(s) 192, 457, 472, 565, 577, 756, 935, 1030
DraIII CACNNNGTG 1 cut(s) 34
EaeI YGGCCR 1 cut(s) 876
Eam1104I CTCTTC 2 cut(s) 638, 662
EarI CTCTTC 2 cut(s) 638, 662
Eco130I CCWWGG 1 cut(s) 806
Eco47I GGWCC 5 cut(s) 129, 170, 429, 678, 695
Eco88I CYCGRG 1 cut(s) 611
EcoRII CCWGG 2 cut(s) 334, 788
EcoT14I CCWWGG 1 cut(s) 806
EcoT22I ATGCAT 1 cut(s) 6
EgeI GGCGCC 2 cut(s) 212, 278
EheI GGCGCC 2 cut(s) 212, 278
ErhI CCWWGG 1 cut(s) 806
FaeI CATG 1 cut(s) 8
FaqI GGGAC 3 cut(s) 71, 183, 897
FatI CATG 1 cut(s) 4
FauI CCCGC 1 cut(s) 744
FauNDI CATATG 1 cut(s) 550
Fnu4HI GCNGC 2 cut(s) 67, 501
FokI GGATG 2 cut(s) 165, 197
Fsp4HI GCNGC 2 cut(s) 67, 501
FspBI CTAG 4 cut(s) 117, 627, 780, 807
FspI TGCGCA 1 cut(s) 513
GlaI GCGC 5 cut(s) 212, 278, 513, 734, 1074
GluI GCNGC 2 cut(s) 67, 501
GsaI CCCAGC 1 cut(s) 919
HaeII RGCGCY 3 cut(s) 214, 280, 1076
HaeIII GGCC 2 cut(s) 500, 878
HapII CCGG 5 cut(s) 78, 182, 214, 612, 760
HgaI GACGC 3 cut(s) 83, 677, 1106
HhaI GCGC 5 cut(s) 213, 279, 514, 735, 1075
Hin1I GRCGYC 5 cut(s) 94, 211, 277, 545, 1117
Hin1II CATG 1 cut(s) 8
Hin6I GCGC 5 cut(s) 211, 277, 512, 733, 1073
HinP1I GCGC 5 cut(s) 211, 277, 512, 733, 1073
HindIII AAGCTT 1 cut(s) 983
HinfI GANTC 2 cut(s) 229, 996
HpaII CCGG 5 cut(s) 78, 182, 214, 612, 760
HphI GGTGA 3 cut(s) 89, 766, 1097
Hpy166II GTNNAC 1 cut(s) 678
Hpy188I TCNGA 3 cut(s) 49, 995, 1030
Hpy188III TCNNGA 6 cut(s) 182, 190, 196, 538, 627, 798
Hpy8I GTNNAC 1 cut(s) 678
Hpy99I CGWCG 3 cut(s) 547, 693, 1122
HpyCH4III ACNGT 4 cut(s) 148, 535, 637, 1041
HpyCH4IV ACGT 1 cut(s) 545
HpyCH4V TGCA 3 cut(s) 4, 152, 841
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 274, 509, 875
HpyF3I CTNAG 1 cut(s) 254
HpySE526I ACGT 1 cut(s) 545
Hsp92I GRCGYC 5 cut(s) 94, 211, 277, 545, 1117
Hsp92II CATG 1 cut(s) 8
HspAI GCGC 5 cut(s) 211, 277, 512, 733, 1073
KasI GGCGCC 2 cut(s) 210, 276
Kpn2I TCCGGA 1 cut(s) 181
KroI GCCGGC 1 cut(s) 213
KroNI GCCGGC 1 cut(s) 215
Kzo9I GATC 8 cut(s) 192, 457, 472, 565, 577, 756, 935, 1030
LmnI GCTCC 1 cut(s) 708
Lsp1109I GCAGC 1 cut(s) 78
LweI GCATC 1 cut(s) 706
MaeI CTAG 4 cut(s) 117, 627, 780, 807
MaeII ACGT 1 cut(s) 545
MaeIII GTNAC 2 cut(s) 95, 403
MalI GATC 8 cut(s) 194, 459, 474, 567, 579, 758, 937, 1032
MbiI CCGCTC 1 cut(s) 1017
MboI GATC 8 cut(s) 192, 457, 472, 565, 577, 756, 935, 1030
MboII GAAGA 5 cut(s) 172, 566, 649, 655, 1080
MflI RGATCY 2 cut(s) 192, 457
MlsI TGGCCA 1 cut(s) 878
MluCI AATT 2 cut(s) 854, 1058
MluNI TGGCCA 1 cut(s) 878
Mly113I GGCGCC 2 cut(s) 211, 277
MlyI GAGTC 1 cut(s) 223
MmeI TCCRAC 1 cut(s) 355
Mox20I TGGCCA 1 cut(s) 878
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 181
MroNI GCCGGC 1 cut(s) 213
MscI TGGCCA 1 cut(s) 878
MslI CAYNNNNRTG 1 cut(s) 183
Msp20I TGGCCA 1 cut(s) 878
MspI CCGG 5 cut(s) 78, 182, 214, 612, 760
MspR9I CCNGG 5 cut(s) 336, 612, 613, 761, 790
MvaI CCWGG 2 cut(s) 336, 790
MvnI CGCG 2 cut(s) 693, 735
MwoI GCNNNNNNNGC 4 cut(s) 149, 274, 509, 875
NaeI GCCGGC 1 cut(s) 215
NarI GGCGCC 2 cut(s) 211, 277
NciI CCSGG 3 cut(s) 612, 613, 761
NdeI CATATG 1 cut(s) 550
NdeII GATC 8 cut(s) 192, 457, 472, 565, 577, 756, 935, 1030
NgoMIV GCCGGC 1 cut(s) 213
NlaIII CATG 1 cut(s) 8
NlaIV GGNNCC 5 cut(s) 171, 212, 218, 278, 813
NmuCI GTSAC 1 cut(s) 95
NsbI TGCGCA 1 cut(s) 513
NsiI ATGCAT 1 cut(s) 6
PdiI GCCGGC 1 cut(s) 215
PfeI GAWTC 1 cut(s) 996
PflFI GACNNNGTC 1 cut(s) 544
PinAI ACCGGT 1 cut(s) 77
PkrI GCNGC 2 cut(s) 68, 502
PleI GAGTC 1 cut(s) 223
PluTI GGCGCC 2 cut(s) 214, 280
PpsI GAGTC 1 cut(s) 223
Psp6I CCWGG 2 cut(s) 334, 788
PspFI CCCAGC 1 cut(s) 915
PspGI CCWGG 2 cut(s) 334, 788
PspN4I GGNNCC 5 cut(s) 171, 212, 218, 278, 813
PspPI GGNCC 5 cut(s) 129, 170, 429, 678, 695
PsuI RGATCY 2 cut(s) 192, 457
PsyI GACNNNGTC 1 cut(s) 544
RsaI GTAC 1 cut(s) 1048
RsaNI GTAC 1 cut(s) 1047
RseI CAYNNNNRTG 1 cut(s) 183
SatI GCNGC 2 cut(s) 67, 501
Sau3AI GATC 8 cut(s) 192, 457, 472, 565, 577, 756, 935, 1030
Sau96I GGNCC 5 cut(s) 129, 170, 429, 678, 695
SchI GAGTC 1 cut(s) 223
ScrFI CCNGG 5 cut(s) 336, 612, 613, 761, 790
SfaNI GCATC 1 cut(s) 706
SfoI GGCGCC 2 cut(s) 212, 278
SinI GGWCC 5 cut(s) 129, 170, 429, 678, 695
SmaI CCCGGG 1 cut(s) 613
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 2 cut(s) 234, 796
SmoI CTYRAG 2 cut(s) 234, 796
Sse9I AATT 2 cut(s) 854, 1058
SsiI CCGC 9 cut(s) 141, 173, 371, 501, 693, 740, 751, 1015, 1054
SspDI GGCGCC 2 cut(s) 210, 276
SspMI CTAG 4 cut(s) 117, 627, 780, 807
StyD4I CCNGG 5 cut(s) 334, 610, 611, 759, 788
StyI CCWWGG 1 cut(s) 806
TaaI ACNGT 4 cut(s) 148, 535, 637, 1041
TaiI ACGT 1 cut(s) 548
TaqI TCGA 3 cut(s) 259, 714, 977
TaqII GACCGA 2 cut(s) 146, 695
TasI AATT 2 cut(s) 854, 1058
TauI GCSGC 1 cut(s) 503
TfiI GAWTC 1 cut(s) 996
TscAI CASTG 1 cut(s) 886
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 1 cut(s) 66
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 2 cut(s) 567, 995
TspMI CCCGGG 1 cut(s) 611
TspRI CASTG 1 cut(s) 886
Tth111I GACNNNGTC 1 cut(s) 544
VpaK11BI GGWCC 5 cut(s) 129, 170, 429, 678, 695
XbaI TCTAGA 1 cut(s) 626
XmaI CCCGGG 1 cut(s) 611
XmaJI CCTAGG 1 cut(s) 806
XspI CTAG 4 cut(s) 117, 627, 780, 807
ZraI GACGTC 1 cut(s) 546
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.