Rroxscaffold_1G00049330

Rab3 gtpase-activating protein non-catalytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013168
Physical Location & Seq
Reverse (-)
69511527 .. 69516261
4735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00049330.1

Sequence Viewer

Length: 1362 bp
ATGGCGAGGCGGGTTCACACGACGGAGGTGGGCTGCATAGCGTGCGAGGAGCTCGGCGAGCTCGGCGCCGGAAAGGAGGGGTGGCTCGTGGACACTCCGAACCTCCTCTGCGCCATCGACACTCACTGCATCGCGCTCGCGAACCGGTCCGTGATTGTGGTACTCGGGTGGGCCGAGCCCGACGGGAGCCGGGTCAAAATCCGACCCGAATTGTCGCCGATTGAGGCCGAGTTCGTGACGGCGATTGAGTGGCTGGTGTTTGATGAGATGCGGGTCGTCGTGGCCGGGACTTCTGCTGGCTATCTCCTCTTTTACTCTTTGGCTGGACATTTGATCCATAAACAGATGGTATATCCAGCTAGGATCATACGGTTGAGGGTCCGAGGAACGAAGAGAGATTTGAGCCACGATACATCCTCGGAAGAGGTTTGTGTTGTTATGCCAGGCATTCTCGCCCGTTTTGATGGTTCGGATATTCAGCACAAATTGTTTCTGGTTTTCTCAGTGGTTTCAAGAAACACAGTCTCATTTTGGGATCGACATCGGAGGTCTGATGATTTGGGAAATTCTTATGGAAAATTACCTTACCAGTTATGGAGTGTCAGCAAGTATGGTAGTTGTGCTGATGCAGCAATCACTGGCATAATGCCTCCACCACTGATGGAAATCCAGTCAAGTGAACATTATTTCTGTGGAATCACTGTTGGAGAGGATGCGGTAATTTCAGCCTTCAGACTTTCGGAAGATAAAAATAGATCTTTCGTGGGAGCTATCTTGTCTAAAGTTGTGCCTGCAACATTTTCAACAATAGCCTCATTCTCAAAACTGATATGGCGAAGTGAACAGACTTCACCTAAGAAATTAGATGAGACACCCCAACCATTTGCTCGAGCTTCTCCATTGACATGTTTGAAGGACTTCCCAAGAAAGGGTGAGAAGCTTACCTTATCACCTAGTGGCACATTGGCTGCAATTACAGATTCACTTGGTCGCATATTGCTTTTAGACACTCAAGCACTTGTCATAGTGCGACTGTGGAAGGGATATCGGGATGCTAGCTGCCTATTCATGGAGATGCTGGTCAATAAAAACACTGCAGCTTCTAGTTCCCGTTATTATGAACCTACCAAGAGTGATTACTGCCTGTGTTTGGTCATTCATGCCCCTAGAAAAGGAATTATTGAGATTTGGCAGATGAGAACTGGACCAAGGCTTCGAAGTTTTGTGTGTGCCAAAGGTAGCAAGATACTGCAACCTACTTACAGATTTGGATCGTCAATAGCCTCTCCTTATGTTCCTTTGGAGGTTTTCTTGTTCAACGGCGACGCTGGTCAGATATCAGTTTTAAATCGAACTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RAB3GAP2_N

Protein Analysis

453

Amino Acids

50.25

Weight (kDa)

8.74

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAB3GAP2_N PF14655 28 - 417 8.6e-117 Rab3 GTPase-activating protein regulatory subunit N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14910
fragaria_vesca FvH4_3g18310 FvH4_3g18310 FvH4_3g18310
malus_domestica MD03G1243500.v1.1
prunus_persica Prupe.4G164800_v2.0.a1
pyrus_communis pycom03g19020
rosa_chinensis RchiOBHm_Chr5g0030941
rosa_laevigata RLG00000033273
rosa_multiflora Rmu_sc0001974.1_g000011
rosa_roxburghii Rroxscaffold_1G00049330
rosa_rugosa Rorug05G0121600 Rorug05G0121600
rosa_samantha Rh5AG214800 Rh5BG213800 Rh5CG237700 Rh5DG218400
rosa_wichuraiana Rw5G019650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 65
AccII CGCG 2 cut(s) 134, 140
AciI CCGC 3 cut(s) 10, 271, 716
AclWI GGATC 4 cut(s) 328, 371, 543, 1279
AcoI YGGCCR 1 cut(s) 282
AcsI RAATTY 1 cut(s) 565
AcuI CTGAAG 1 cut(s) 715
AcyI GRCGYC 1 cut(s) 66
AdeI CACNNNGTG 1 cut(s) 956
AfaI GTAC 1 cut(s) 162
AfiI CCNNNNNNNGG 5 cut(s) 928, 929, 1069, 1150, 1172
AflIII ACRYGT 1 cut(s) 905
AgeI ACCGGT 1 cut(s) 144
AgsI TTSAA 4 cut(s) 513, 804, 913, 1318
AjnI CCWGG 1 cut(s) 442
AluBI AGCT 8 cut(s) 52, 61, 359, 770, 893, 940, 1059, 1100
AluI AGCT 8 cut(s) 52, 61, 359, 770, 893, 940, 1059, 1100
Alw21I GWGCWC 2 cut(s) 54, 63
Alw26I GTCTC 2 cut(s) 529, 863
AlwI GGATC 4 cut(s) 328, 371, 543, 1279
Ama87I CYCGRG 2 cut(s) 164, 888
AoxI GGCC 3 cut(s) 171, 225, 282
ApeKI GCWGC 5 cut(s) 33, 629, 968, 1059, 1097
ApoI RAATTY 1 cut(s) 565
ArsI GACNNNNNNTTYG 2 cut(s) 1197, 1229
AsiGI ACCGGT 1 cut(s) 144
Asp700I GAANNNNTTC 1 cut(s) 917
AspLEI GCGC 3 cut(s) 68, 113, 136
AspS9I GGNCC 4 cut(s) 147, 171, 379, 1205
AsuC2I CCSGG 2 cut(s) 191, 286
AsuHPI GGTGA 3 cut(s) 843, 942, 944
AsuII TTCGAA 1 cut(s) 1216
AsuNHI GCTAGC 1 cut(s) 1055
AvaI CYCGRG 2 cut(s) 164, 888
AvaII GGWCC 3 cut(s) 147, 379, 1205
BaeI ACNNNNGTAYC 2 cut(s) 152, 185
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 3 cut(s) 54, 63, 180
BauI CACGAG 1 cut(s) 86
Bbv12I GWGCWC 2 cut(s) 54, 63
BbvI GCAGC 5 cut(s) 20, 641, 955, 1046, 1109
BccI CCATC 4 cut(s) 122, 340, 458, 655
BceAI ACGGC 2 cut(s) 255, 1336
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 1 cut(s) 444
BcnI CCSGG 2 cut(s) 191, 286
BcoDI GTCTC 2 cut(s) 529, 863
BfaI CTAG 5 cut(s) 360, 954, 1056, 1104, 1167
BfmI CTRYAG 1 cut(s) 1095
BfoI RGCGCY 1 cut(s) 69
BglII AGATCT 1 cut(s) 755
BisI GCNGC 5 cut(s) 34, 630, 969, 1060, 1098
BlsI GCNGC 5 cut(s) 35, 631, 970, 1061, 1099
Bme1390I CCNGG 3 cut(s) 191, 286, 444
Bme18I GGWCC 3 cut(s) 147, 379, 1205
BmeT110I CYCGRG 2 cut(s) 164, 888
BmgT120I GGNCC 4 cut(s) 147, 171, 379, 1205
BmiI GGNNCC 3 cut(s) 67, 188, 380
BmrFI CCNGG 3 cut(s) 191, 286, 444
BmsI GCATC 6 cut(s) 138, 258, 616, 703, 1042, 1065
BmtI GCTAGC 1 cut(s) 1059
BoxI GACNNNNGTC 1 cut(s) 1329
Bpu14I TTCGAA 1 cut(s) 1216
BpuEI CTTGAG 1 cut(s) 996
BpuMI CCSGG 2 cut(s) 191, 286
BsaBI GATNNNNATC 3 cut(s) 540, 665, 1270
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 3 cut(s) 382, 417, 1208
BsaWI WCCGGW 1 cut(s) 144
Bsc4I CCNNNNNNNGG 5 cut(s) 928, 929, 1069, 1150, 1172
Bse118I RCCGGY 1 cut(s) 144
Bse1I ACTGG 4 cut(s) 589, 643, 670, 1207
Bse8I GATNNNNATC 3 cut(s) 540, 665, 1270
BseBI CCWGG 1 cut(s) 444
BseDI CCNNGG 3 cut(s) 382, 417, 1208
BseGI GGATG 3 cut(s) 413, 718, 1057
BseJI GATNNNNATC 3 cut(s) 540, 665, 1270
BseLI CCNNNNNNNGG 5 cut(s) 928, 929, 1069, 1150, 1172
BseMII CTCAG 1 cut(s) 516
BseNI ACTGG 4 cut(s) 589, 643, 670, 1207
BseRI GAGGAG 3 cut(s) 62, 95, 296
BseXI GCAGC 5 cut(s) 20, 641, 955, 1046, 1109
Bsh1236I CGCG 2 cut(s) 134, 140
BshFI GGCC 3 cut(s) 173, 227, 284
BshNI GGYRCC 1 cut(s) 65
BshTI ACCGGT 1 cut(s) 144
BsiHKAI GWGCWC 2 cut(s) 54, 63
BsiHKCI CYCGRG 2 cut(s) 164, 888
BsiSI CCGG 4 cut(s) 69, 145, 190, 285
BslFI GGGAC 1 cut(s) 301
BslI CCNNNNNNNGG 5 cut(s) 928, 929, 1069, 1150, 1172
BsmAI GTCTC 2 cut(s) 529, 863
BsmFI GGGAC 1 cut(s) 301
BsmI GAATGC 1 cut(s) 447
BsnI GGCC 3 cut(s) 173, 227, 284
BsoBI CYCGRG 2 cut(s) 164, 888
Bsp119I TTCGAA 1 cut(s) 1216
Bsp1286I GDGCHC 3 cut(s) 54, 63, 180
Bsp143I GATC 5 cut(s) 333, 363, 535, 755, 1271
Bsp68I TCGCGA 1 cut(s) 140
BspACI CCGC 3 cut(s) 10, 271, 716
BspANI GGCC 3 cut(s) 173, 227, 284
BspCNI CTCAG 1 cut(s) 515
BspFNI CGCG 2 cut(s) 134, 140
BspLI GGNNCC 3 cut(s) 67, 188, 380
BspMAI CTGCAG 1 cut(s) 1099
BspOI GCTAGC 1 cut(s) 1059
BspPI GGATC 4 cut(s) 328, 371, 543, 1279
BspT104I TTCGAA 1 cut(s) 1216
BspT107I GGYRCC 1 cut(s) 65
BsrFI RCCGGY 1 cut(s) 144
BsrI ACTGG 4 cut(s) 589, 643, 670, 1207
BssAI RCCGGY 1 cut(s) 144
BssECI CCNNGG 3 cut(s) 382, 417, 1208
BssMI GATC 5 cut(s) 333, 363, 535, 755, 1271
BssNI GRCGYC 1 cut(s) 66
BssSI CACGAG 1 cut(s) 86
BssT1I CCWWGG 1 cut(s) 1208
Bst2BI CACGAG 1 cut(s) 86
Bst2UI CCWGG 1 cut(s) 444
Bst4CI ACNGT 4 cut(s) 372, 523, 703, 1035
Bst6I CTCTTC 2 cut(s) 386, 417
BstACI GRCGYC 1 cut(s) 66
BstAPI GCANNNNNTGC 1 cut(s) 42
BstBI TTCGAA 1 cut(s) 1216
BstC8I GCNNGC 6 cut(s) 43, 59, 138, 298, 792, 1057
BstDEI CTNAG 2 cut(s) 502, 855
BstENI CCTNNNNNAGG 1 cut(s) 1170
BstF5I GGATG 3 cut(s) 413, 718, 1057
BstFNI CGCG 2 cut(s) 134, 140
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 3 cut(s) 68, 113, 136
BstKTI GATC 5 cut(s) 336, 366, 538, 758, 1274
BstMAI GTCTC 2 cut(s) 529, 863
BstMBI GATC 5 cut(s) 333, 363, 535, 755, 1271
BstMWI GCNNNNNNNGC 4 cut(s) 42, 58, 63, 629
BstNI CCWGG 1 cut(s) 444
BstNSI RCATGY 1 cut(s) 909
BstPAI GACNNNNGTC 1 cut(s) 1329
BstSCI CCNGG 3 cut(s) 189, 284, 442
BstSFI CTRYAG 1 cut(s) 1095
BstUI CGCG 2 cut(s) 134, 140
BstV1I GCAGC 5 cut(s) 20, 641, 955, 1046, 1109
BstX2I RGATCY 1 cut(s) 755
BstYI RGATCY 1 cut(s) 755
BsuRI GGCC 3 cut(s) 173, 227, 284
BtgZI GCGATG 1 cut(s) 115
BtsCI GGATG 3 cut(s) 413, 718, 1057
BtsI GCAGTG 2 cut(s) 124, 1092
BtsIMutI CAGTG 6 cut(s) 124, 510, 636, 656, 699, 1092
BtuMI TCGCGA 1 cut(s) 140
Cac8I GCNNGC 6 cut(s) 43, 59, 138, 298, 792, 1057
CfoI GCGC 3 cut(s) 68, 113, 136
Cfr10I RCCGGY 1 cut(s) 144
Cfr13I GGNCC 4 cut(s) 147, 171, 379, 1205
CpoI CGGWCCG 1 cut(s) 147
CseI GACGC 1 cut(s) 1334
Csp6I GTAC 1 cut(s) 161
CspAI ACCGGT 1 cut(s) 144
CspI CGGWCCG 1 cut(s) 147
CviAII CATG 3 cut(s) 906, 1069, 1160
CviQI GTAC 1 cut(s) 161
DdeI CTNAG 2 cut(s) 502, 855
DinI GGCGCC 1 cut(s) 67
DpnI GATC 5 cut(s) 335, 365, 537, 757, 1273
DpnII GATC 5 cut(s) 333, 363, 535, 755, 1271
DraI TTTAAA 1 cut(s) 1347
DraIII CACNNNGTG 1 cut(s) 956
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 2 cut(s) 386, 417
EarI CTCTTC 2 cut(s) 386, 417
Ecl136II GAGCTC 2 cut(s) 52, 61
Eco130I CCWWGG 1 cut(s) 1208
Eco24I GRGCYC 3 cut(s) 54, 63, 180
Eco32I GATATC 2 cut(s) 1046, 1338
Eco47I GGWCC 3 cut(s) 147, 379, 1205
Eco53kI GAGCTC 2 cut(s) 52, 61
Eco57I CTGAAG 1 cut(s) 715
Eco88I CYCGRG 2 cut(s) 164, 888
EcoICRI GAGCTC 2 cut(s) 52, 61
EcoNI CCTNNNNNAGG 1 cut(s) 1170
EcoRII CCWGG 1 cut(s) 442
EcoRV GATATC 2 cut(s) 1046, 1338
EcoT14I CCWWGG 1 cut(s) 1208
EcoT38I GRGCYC 3 cut(s) 54, 63, 180
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 1208
FaeI CATG 3 cut(s) 909, 1072, 1163
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 1 cut(s) 301
FatI CATG 3 cut(s) 905, 1068, 1159
FauI CCCGC 2 cut(s) 3, 264
Fnu4HI GCNGC 5 cut(s) 34, 630, 969, 1060, 1098
FokI GGATG 3 cut(s) 400, 725, 1064
FriOI GRGCYC 3 cut(s) 54, 63, 180
Fsp4HI GCNGC 5 cut(s) 34, 630, 969, 1060, 1098
FspBI CTAG 5 cut(s) 360, 954, 1056, 1104, 1167
GlaI GCGC 3 cut(s) 67, 112, 135
GluI GCNGC 5 cut(s) 34, 630, 969, 1060, 1098
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 3 cut(s) 173, 227, 284
HapII CCGG 4 cut(s) 69, 145, 190, 285
HgaI GACGC 1 cut(s) 1334
HhaI GCGC 3 cut(s) 68, 113, 136
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 3 cut(s) 909, 1072, 1163
Hin6I GCGC 3 cut(s) 66, 111, 134
HinP1I GCGC 3 cut(s) 66, 111, 134
HindIII AAGCTT 1 cut(s) 938
HinfI GANTC 2 cut(s) 696, 980
HpaII CCGG 4 cut(s) 69, 145, 190, 285
HphI GGTGA 3 cut(s) 843, 942, 944
Hpy166II GTNNAC 4 cut(s) 16, 91, 680, 842
Hpy188III TCNNGA 4 cut(s) 139, 235, 513, 1049
Hpy8I GTNNAC 4 cut(s) 16, 91, 680, 842
Hpy99I CGWCG 4 cut(s) 25, 185, 281, 1328
HpyAV CCTTC 3 cut(s) 739, 907, 1033
HpyCH4III ACNGT 4 cut(s) 372, 523, 703, 1035
HpyCH4V TGCA 7 cut(s) 36, 129, 629, 794, 971, 1097, 1252
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 58, 63, 629
HpyF3I CTNAG 2 cut(s) 502, 855
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 3 cut(s) 909, 1072, 1163
HspAI GCGC 3 cut(s) 66, 111, 134
KasI GGCGCC 1 cut(s) 65
Kzo9I GATC 5 cut(s) 333, 363, 535, 755, 1271
LmnI GCTCC 3 cut(s) 49, 186, 767
Lsp1109I GCAGC 5 cut(s) 20, 641, 955, 1046, 1109
LweI GCATC 6 cut(s) 138, 258, 616, 703, 1042, 1065
MaeI CTAG 5 cut(s) 360, 954, 1056, 1104, 1167
MaeIII GTNAC 1 cut(s) 235
MalI GATC 5 cut(s) 335, 365, 537, 757, 1273
MboI GATC 5 cut(s) 333, 363, 535, 755, 1271
MboII GAAGA 3 cut(s) 403, 434, 755
MflI RGATCY 1 cut(s) 755
MhlI GDGCHC 3 cut(s) 54, 63, 180
MluCI AATT 8 cut(s) 209, 485, 565, 578, 720, 860, 972, 1176
Mly113I GGCGCC 1 cut(s) 66
MmeI TCCRAC 2 cut(s) 226, 685
MroXI GAANNNNTTC 1 cut(s) 917
MseI TTAA 2 cut(s) 1346, 1360
MslI CAYNNNNRTG 2 cut(s) 904, 1073
MspI CCGG 4 cut(s) 69, 145, 190, 285
MspR9I CCNGG 3 cut(s) 191, 286, 444
Mva1269I GAATGC 1 cut(s) 447
MvaI CCWGG 1 cut(s) 444
MvnI CGCG 2 cut(s) 134, 140
MwoI GCNNNNNNNGC 4 cut(s) 42, 58, 63, 629
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 2 cut(s) 191, 286
NdeII GATC 5 cut(s) 333, 363, 535, 755, 1271
NheI GCTAGC 1 cut(s) 1055
NlaIII CATG 3 cut(s) 909, 1072, 1163
NlaIV GGNNCC 3 cut(s) 67, 188, 380
NmeAIII GCCGAG 4 cut(s) 33, 42, 199, 253
NmuCI GTSAC 1 cut(s) 235
NruI TCGCGA 1 cut(s) 140
NspI RCATGY 1 cut(s) 909
NspV TTCGAA 1 cut(s) 1216
PaeR7I CTCGAG 1 cut(s) 888
PciI ACATGT 1 cut(s) 905
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PctI GAATGC 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 917
PfeI GAWTC 2 cut(s) 696, 980
PinAI ACCGGT 1 cut(s) 144
PkrI GCNGC 5 cut(s) 35, 631, 970, 1061, 1099
PluTI GGCGCC 1 cut(s) 69
PscI ACATGT 1 cut(s) 905
PshAI GACNNNNGTC 1 cut(s) 1329
Psp124BI GAGCTC 2 cut(s) 54, 63
Psp6I CCWGG 1 cut(s) 442
PspGI CCWGG 1 cut(s) 442
PspN4I GGNNCC 3 cut(s) 67, 188, 380
PspPI GGNCC 4 cut(s) 147, 171, 379, 1205
PspXI VCTCGAGB 1 cut(s) 888
PstI CTGCAG 1 cut(s) 1099
PsuI RGATCY 1 cut(s) 755
RruI TCGCGA 1 cut(s) 140
RsaI GTAC 1 cut(s) 162
RsaNI GTAC 1 cut(s) 161
RseI CAYNNNNRTG 2 cut(s) 904, 1073
Rsr2I CGGWCCG 1 cut(s) 147
RsrII CGGWCCG 1 cut(s) 147
SacI GAGCTC 2 cut(s) 54, 63
SaqAI TTAA 2 cut(s) 1346, 1360
SatI GCNGC 5 cut(s) 34, 630, 969, 1060, 1098
Sau3AI GATC 5 cut(s) 333, 363, 535, 755, 1271
Sau96I GGNCC 4 cut(s) 147, 171, 379, 1205
ScrFI CCNGG 3 cut(s) 191, 286, 444
SduI GDGCHC 3 cut(s) 54, 63, 180
SfaNI GCATC 6 cut(s) 138, 258, 616, 703, 1042, 1065
SfcI CTRYAG 1 cut(s) 1095
SfoI GGCGCC 1 cut(s) 67
Sfr274I CTCGAG 1 cut(s) 888
SfuI TTCGAA 1 cut(s) 1216
SinI GGWCC 3 cut(s) 147, 379, 1205
SlaI CTCGAG 1 cut(s) 888
SmiMI CAYNNNNRTG 2 cut(s) 904, 1073
SmlI CTYRAG 2 cut(s) 888, 1011
SmoI CTYRAG 2 cut(s) 888, 1011
Sse9I AATT 8 cut(s) 209, 485, 565, 578, 720, 860, 972, 1176
SsiI CCGC 3 cut(s) 10, 271, 716
SspDI GGCGCC 1 cut(s) 65
SspMI CTAG 5 cut(s) 360, 954, 1056, 1104, 1167
SstI GAGCTC 2 cut(s) 54, 63
StyD4I CCNGG 3 cut(s) 189, 284, 442
StyI CCWWGG 1 cut(s) 1208
TaaI ACNGT 4 cut(s) 372, 523, 703, 1035
TaqI TCGA 5 cut(s) 117, 538, 889, 1216, 1351
TasI AATT 8 cut(s) 209, 485, 565, 578, 720, 860, 972, 1176
TfiI GAWTC 2 cut(s) 696, 980
Tru1I TTAA 2 cut(s) 1346, 1360
Tru9I TTAA 2 cut(s) 1346, 1360
TscAI CASTG 6 cut(s) 131, 510, 643, 663, 706, 1099
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 5 cut(s) 33, 629, 968, 1059, 1097
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 3 cut(s) 1057, 1134, 1148
TspGWI ACGGA 2 cut(s) 38, 139
TspRI CASTG 6 cut(s) 131, 510, 643, 663, 706, 1099
VpaK11BI GGWCC 3 cut(s) 147, 379, 1205
XagI CCTNNNNNAGG 1 cut(s) 1170
XapI RAATTY 1 cut(s) 565
XceI RCATGY 1 cut(s) 909
XhoI CTCGAG 1 cut(s) 888
XmnI GAANNNNTTC 1 cut(s) 917
XspI CTAG 5 cut(s) 360, 954, 1056, 1104, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.