Rroxscaffold_2G00078160

Lysine histidine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0021281
Physical Location & Seq
Reverse (-)
1562399 .. 1565477
3079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00078160.1

Sequence Viewer

Length: 546 bp
ATGGACGAAAAATTAGCCAAGGAAAAGGCAATTAATGAGTGGCTTCCAATTACTTCTTCAAGGAATGCAAAATGGTGGTATTCGGCTTTTCACAATGTTACGGCCATGGTTGGAGCTGGTGTTCTCGGTCTCCCTTATGCCATGGCAGAGCTTGGATGGGGTCCTGGTATAGCTATTTTGGTCCTTTCATGGGTCATCACATTATACACACTATGGCAAATGGTTGAGATGCATGAGATGGTTCCAGGGAAGCGTTTTGATCGTTACCACGAATTGGGTCAGCATGCATTTGGTGAAAAACTTGGTCTTTACATTGTGGTGCCTCAGCAACTCATTGTTGAAGTTGGTGTTTGCATTGTGTACATGGTCACCGGAGGAAGATCGCTGCAGAAGGTCCATGACACTGTGTGCTCAGACTGCAAAAAAATCAAATTGACTTACTTCATCATGATCTTTGCCTCTGTTCACTTTGTGCTTTCCCACCTTCCCAACTTCAACTCCATCTCCGGCGTGTCTTTGGCCGCAGCTGTCATGTCCTTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

181

Amino Acids

20.27

Weight (kDa)

8.42

Isoelectric Point (pI)

30.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 22 - 180 1.5e-50 Transmembrane amino acid transporter protein
Trp_Tyr_perm PF03222 26 - 158 5.4e-11 Tryptophan/tyrosine permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021281)

Species Orthologous Gene IDs
pyrus_communis pycom13g09700
rosa_multiflora Rmu_sc0003649.1_g000019
rosa_roxburghii Rroxscaffold_2G00078120 Rroxscaffold_2G00078160 Rroxscaffold_5G00372790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 319
AccB7I CCANNNNNTGG 1 cut(s) 274
AciI CCGC 1 cut(s) 522
AcoI YGGCCR 2 cut(s) 102, 519
AdeI CACNNNGTG 2 cut(s) 408, 472
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 2 cut(s) 190, 274
AgsI TTSAA 3 cut(s) 60, 341, 496
AjnI CCWGG 2 cut(s) 163, 244
AjuI GAANNNNNNNTTGG 2 cut(s) 40, 72
AloI GAACNNNNNNTCC 2 cut(s) 105, 137
AluBI AGCT 4 cut(s) 116, 151, 173, 527
AluI AGCT 4 cut(s) 116, 151, 173, 527
Alw21I GWGCWC 1 cut(s) 413
Alw26I GTCTC 1 cut(s) 134
AoxI GGCC 2 cut(s) 102, 519
ApeKI GCWGC 2 cut(s) 385, 524
AseI ATTAAT 1 cut(s) 33
AspS9I GGNCC 3 cut(s) 161, 181, 394
AsuHPI GGTGA 2 cut(s) 305, 361
AvaII GGWCC 3 cut(s) 161, 181, 394
BanI GGYRCC 1 cut(s) 319
Bbv12I GWGCWC 1 cut(s) 413
BbvCI CCTCAGC 1 cut(s) 324
BbvI GCAGC 2 cut(s) 372, 536
BccI CCATC 3 cut(s) 150, 232, 509
BceAI ACGGC 1 cut(s) 117
BciT130I CCWGG 2 cut(s) 165, 246
BcoDI GTCTC 1 cut(s) 134
BfmI CTRYAG 1 cut(s) 386
BisI GCNGC 3 cut(s) 386, 522, 525
BlsI GCNGC 3 cut(s) 387, 523, 526
Bme1390I CCNGG 2 cut(s) 165, 246
Bme18I GGWCC 3 cut(s) 161, 181, 394
BmgT120I GGNCC 3 cut(s) 161, 181, 394
BmiI GGNNCC 3 cut(s) 162, 243, 321
BmrFI CCNGG 2 cut(s) 165, 246
BmsI GCATC 1 cut(s) 219
Bpu10I CCTNAGC 1 cut(s) 324
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 4 cut(s) 18, 105, 141, 245
BsaWI WCCGGW 1 cut(s) 371
BsaXI ACNNNNNCTCC 6 cut(s) 105, 135, 482, 488, 512, 518
Bsc4I CCNNNNNNNGG 2 cut(s) 190, 274
BseBI CCWGG 2 cut(s) 165, 246
BseDI CCNNGG 4 cut(s) 18, 105, 141, 245
BseGI GGATG 1 cut(s) 161
BseLI CCNNNNNNNGG 2 cut(s) 190, 274
BseMII CTCAG 2 cut(s) 338, 426
BseXI GCAGC 2 cut(s) 372, 536
BshFI GGCC 2 cut(s) 104, 521
BshNI GGYRCC 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 413
BsiSI CCGG 2 cut(s) 372, 507
BslI CCNNNNNNNGG 2 cut(s) 190, 274
BsmAI GTCTC 1 cut(s) 134
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 2 cut(s) 104, 521
Bso31I GGTCTC 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 413
Bsp1407I TGTACA 1 cut(s) 360
Bsp143I GATC 3 cut(s) 259, 380, 450
Bsp19I CCATGG 2 cut(s) 105, 141
BspACI CCGC 1 cut(s) 522
BspANI GGCC 2 cut(s) 104, 521
BspCNI CTCAG 2 cut(s) 337, 425
BspHI TCATGA 1 cut(s) 447
BspLI GGNNCC 3 cut(s) 162, 243, 321
BspMAI CTGCAG 1 cut(s) 390
BspT107I GGYRCC 1 cut(s) 319
BspTNI GGTCTC 1 cut(s) 134
BsrGI TGTACA 1 cut(s) 360
BssECI CCNNGG 4 cut(s) 18, 105, 141, 245
BssMI GATC 3 cut(s) 259, 380, 450
BssT1I CCWWGG 3 cut(s) 18, 105, 141
Bst2UI CCWGG 2 cut(s) 165, 246
Bst4CI ACNGT 1 cut(s) 406
BstAUI TGTACA 1 cut(s) 360
BstC8I GCNNGC 1 cut(s) 285
BstDEI CTNAG 2 cut(s) 324, 412
BstDSI CCRYGG 2 cut(s) 105, 141
BstEII GGTNACC 1 cut(s) 367
BstF5I GGATG 1 cut(s) 161
BstKTI GATC 3 cut(s) 262, 383, 453
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 3 cut(s) 259, 380, 450
BstMWI GCNNNNNNNGC 1 cut(s) 417
BstNI CCWGG 2 cut(s) 165, 246
BstNSI RCATGY 1 cut(s) 287
BstPI GGTNACC 1 cut(s) 367
BstSCI CCNGG 2 cut(s) 163, 244
BstSFI CTRYAG 1 cut(s) 386
BstV1I GCAGC 2 cut(s) 372, 536
BsuRI GGCC 2 cut(s) 104, 521
BtgI CCRYGG 2 cut(s) 105, 141
BtsCI GGATG 1 cut(s) 161
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 1 cut(s) 285
CciI TCATGA 1 cut(s) 447
Cfr13I GGNCC 3 cut(s) 161, 181, 394
Csp6I GTAC 1 cut(s) 361
CviAII CATG 9 cut(s) 106, 142, 189, 233, 284, 364, 398, 448, 532
CviJI RGCY 9 cut(s) 17, 43, 86, 104, 116, 151, 173, 521, 527
CviKI_1 RGCY 9 cut(s) 17, 43, 86, 104, 116, 151, 173, 521, 527
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 2 cut(s) 324, 412
DpnI GATC 3 cut(s) 261, 382, 452
DpnII GATC 3 cut(s) 259, 380, 450
DraIII CACNNNGTG 2 cut(s) 408, 472
EaeI YGGCCR 2 cut(s) 102, 519
Eco130I CCWWGG 3 cut(s) 18, 105, 141
Eco31I GGTCTC 1 cut(s) 134
Eco47I GGWCC 3 cut(s) 161, 181, 394
Eco91I GGTNACC 1 cut(s) 367
EcoO109I RGGNCCY 1 cut(s) 161
EcoO65I GGTNACC 1 cut(s) 367
EcoRII CCWGG 2 cut(s) 163, 244
EcoT14I CCWWGG 3 cut(s) 18, 105, 141
EcoT22I ATGCAT 2 cut(s) 234, 289
ErhI CCWWGG 3 cut(s) 18, 105, 141
FaeI CATG 9 cut(s) 109, 145, 192, 236, 287, 367, 401, 451, 535
FatI CATG 9 cut(s) 105, 141, 188, 232, 283, 363, 397, 447, 531
Fnu4HI GCNGC 3 cut(s) 386, 522, 525
FokI GGATG 1 cut(s) 168
Fsp4HI GCNGC 3 cut(s) 386, 522, 525
GluI GCNGC 3 cut(s) 386, 522, 525
HaeIII GGCC 2 cut(s) 104, 521
HapII CCGG 2 cut(s) 372, 507
Hin1II CATG 9 cut(s) 109, 145, 192, 236, 287, 367, 401, 451, 535
HpaII CCGG 2 cut(s) 372, 507
HphI GGTGA 2 cut(s) 305, 361
Hpy166II GTNNAC 2 cut(s) 361, 466
Hpy188I TCNGA 1 cut(s) 415
Hpy188III TCNNGA 1 cut(s) 448
Hpy8I GTNNAC 2 cut(s) 361, 466
HpyAV CCTTC 2 cut(s) 385, 494
HpyCH4III ACNGT 1 cut(s) 406
HpyCH4V TGCA 6 cut(s) 68, 232, 287, 354, 388, 420
HpyF10VI GCNNNNNNNGC 1 cut(s) 417
HpyF3I CTNAG 2 cut(s) 324, 412
Hsp92II CATG 9 cut(s) 109, 145, 192, 236, 287, 367, 401, 451, 535
Kzo9I GATC 3 cut(s) 259, 380, 450
LmnI GCTCC 1 cut(s) 113
LpnPI CCDG 7 cut(s) 102, 150, 177, 231, 258, 385, 520
Lsp1109I GCAGC 2 cut(s) 372, 536
LweI GCATC 1 cut(s) 219
MaeIII GTNAC 3 cut(s) 97, 263, 367
MalI GATC 3 cut(s) 261, 382, 452
MboI GATC 3 cut(s) 259, 380, 450
MboII GAAGA 2 cut(s) 48, 390
MhlI GDGCHC 1 cut(s) 413
MluCI AATT 5 cut(s) 11, 30, 48, 272, 431
MmeI TCCRAC 1 cut(s) 91
MnlI CCTC 4 cut(s) 333, 368, 469, 534
Mph1103I ATGCAT 2 cut(s) 234, 289
MseI TTAA 1 cut(s) 33
MslI CAYNNNNRTG 1 cut(s) 317
MspA1I CMGCKG 1 cut(s) 527
MspI CCGG 2 cut(s) 372, 507
MspR9I CCNGG 2 cut(s) 165, 246
Mva1269I GAATGC 1 cut(s) 70
MvaI CCWGG 2 cut(s) 165, 246
MwoI GCNNNNNNNGC 1 cut(s) 417
NcoI CCATGG 2 cut(s) 105, 141
NdeII GATC 3 cut(s) 259, 380, 450
NlaIII CATG 9 cut(s) 109, 145, 192, 236, 287, 367, 401, 451, 535
NlaIV GGNNCC 3 cut(s) 162, 243, 321
NmuCI GTSAC 1 cut(s) 367
NsiI ATGCAT 2 cut(s) 234, 289
NspI RCATGY 1 cut(s) 287
PaeI GCATGC 1 cut(s) 287
PagI TCATGA 1 cut(s) 447
PctI GAATGC 1 cut(s) 70
PflMI CCANNNNNTGG 1 cut(s) 274
PkrI GCNGC 3 cut(s) 387, 523, 526
PpuMI RGGWCCY 1 cut(s) 161
PshBI ATTAAT 1 cut(s) 33
Psp5II RGGWCCY 1 cut(s) 161
Psp6I CCWGG 2 cut(s) 163, 244
PspEI GGTNACC 1 cut(s) 367
PspGI CCWGG 2 cut(s) 163, 244
PspN4I GGNNCC 3 cut(s) 162, 243, 321
PspPI GGNCC 3 cut(s) 161, 181, 394
PspPPI RGGWCCY 1 cut(s) 161
PstI CTGCAG 1 cut(s) 390
PvuII CAGCTG 1 cut(s) 527
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
RseI CAYNNNNRTG 1 cut(s) 317
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 3 cut(s) 386, 522, 525
Sau3AI GATC 3 cut(s) 259, 380, 450
Sau96I GGNCC 3 cut(s) 161, 181, 394
ScrFI CCNGG 2 cut(s) 165, 246
SduI GDGCHC 1 cut(s) 413
SetI ASST 7 cut(s) 118, 153, 175, 396, 486, 529, 545
SfaNI GCATC 1 cut(s) 219
SfcI CTRYAG 1 cut(s) 386
SinI GGWCC 3 cut(s) 161, 181, 394
SmiMI CAYNNNNRTG 1 cut(s) 317
SmlI CTYRAG 1 cut(s) 538
SmoI CTYRAG 1 cut(s) 538
SphI GCATGC 1 cut(s) 287
Sse9I AATT 5 cut(s) 11, 30, 48, 272, 431
SsiI CCGC 1 cut(s) 522
StyD4I CCNGG 2 cut(s) 163, 244
StyI CCWWGG 3 cut(s) 18, 105, 141
TaaI ACNGT 1 cut(s) 406
TaqII GACCGA 1 cut(s) 116
TasI AATT 5 cut(s) 11, 30, 48, 272, 431
TatI WGTACW 1 cut(s) 360
TauI GCSGC 1 cut(s) 524
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 2 cut(s) 385, 524
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 2 cut(s) 177, 433
TspRI CASTG 1 cut(s) 409
Van91I CCANNNNNTGG 1 cut(s) 274
VpaK11BI GGWCC 3 cut(s) 161, 181, 394
VspI ATTAAT 1 cut(s) 33
XceI RCATGY 1 cut(s) 287
Zsp2I ATGCAT 2 cut(s) 234, 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.