Rroxscaffold_2G00081010
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014780
Physical Location & Seq
Reverse (-)
3996303 .. 3998669
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00081010.1

Sequence Viewer

Length: 1839 bp
ATGAACAGAATTTCCGTCTGCGTAATCTTCCTTTCATTGTTTCTCTTCCTCCACAGCACTAGTTCTAAAGTGGATGATGAGACCAAGGATTCACTACTCTCCTTCCTTGATAAACTTTCAAACGGTGCTACTCAACCTGGATTCACTTTGGGTTGGACCACCTTGTCTGATCCCTGTGAGAAACAATGGGGAGGTATAACATGTGATTCCCAAAAAACTTCTGTCACAGGACTCTTTCTAAACAAACAAACCCTCATTGGAACCCTTCATGTTGCTACCCTTTGCAATGTGAAATCTGTTGCTGCCTCTCTCACCATTCTTGACCTTAATGACAACAGCATTGGGGGAGAAATTTCAGCTGATATTCAGAACTGTCAGCAGCTCACTAGATTGAATGTAAGCGGAAACCGGCTTTCAGGAAACATTCCTGATTCACTTGCCATGTTAAGCAACCTGAGAAGGCTTGACATTTCCTACAATGACTTCTCTGGCAAGTTACCTGAGTTGTCTTTGATTTCAGGCCTTACAGATTTCCTTGCACAAAATAACCAGCTTACTGGGGAGATACCAAAATTCAACTTCTCTCATTTTGACACCTTCAATGTCTCCAACAATAACTTTCATGGTCCAATCCAGAATGCGCGAATGGCAATGTCTCTGCAAGCAGCTTCCTTGGTAATCCTGGATTATGTGGATATCCACTGCCAAACAACTGTTCAACAACTTCTTCTTCTTCTACAGCAACAGCGGTGTTACTCATTTCCCAGGATATGTAGCAAAAAAGAAAGAAGAAAAAATGATAAGAAGGCTGTTGATGCACCTGAAAAGAACAAGGTAGCATCAGTTGATGAAAGCATGGACAGCAAATATAGTGCTTCATCGACTGACTTCAAAGGCGGTTTCAGCAAATCACAATGTTCAGTAACTTTCTCATCCGAGAGCAATGCTAATATGACCTCCTCCTCACTTGTTGTCCTCTCCAGCCCTGTGGTCAATGGATTGAACTTTGAGGAGTTGCTCAAAGCTCCTGCTGAGATGCTTGGAAGAGGCAAGCATGGTAGCCTGTACAAGGTCATCATTGATTATGGGGCGACCCTTGTTGTCAAAAGGATTAAAGATTGGACGATTTCGACTAATGATTTCAAGCTGAGGATGCAGAGGTTAGACCAAGCCAAGCACCCTAATGTATTGTCTGCTCTTGCCTTTTACTCCTCCAGACAAGAAAAGCTTCTGGTCTATGAATATCAGTACAATGGAAGCCTTTTCAGGCTCATACACGGAAACCAAGGGGGCACAGCCTTTGGCTGGACTAGCAGACTTTCTTGTGCAGCAACCATTGCCGACACATTAGCTTTTATGCATGAGGATCTTCAAAAAGATGGGATTGTTCATGGAAACTTAAAATCTTCGAACATTTTGTTGAACAAGAACATGGAGCCTTGCATAAGCGAGTATGGTCTTATGAAGATCAACGATCATGATAATATATTGCCGGGGAAGGTGTCTGCTGCCAGTAGTGCTTCTACCACATTTAAACAAGACATTTATGGGTTTGGTGTGATTCTTCTTGAGCTACTTACAGGCAAACTTGTACAGCACAACGGAGTTGACTTGACATCTTGGGTTCACTCGGTGGTTCGAGAGGAATGGACTGCAGAAGTGTTTGACAAGTCCTTATATTCGGAAGGTGCAAGCGAGGAGAGGATGGTGAACTTGCTCCAAGTGGCTATAAAGTGTGTCAACCGTTCTCCAGAGGCTAGACCGAGCATGAAACGAGTTGCTACCATGATCAATACCATAAGAGAGGATGAAGACAAATCCACATTCTTTGATGTATGA

Protein Analysis

612

Amino Acids

67.73

Weight (kDa)

6.68

Isoelectric Point (pI)

30.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 103 - 162 2.1e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 345 - 595 5.1e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 345 - 593 3.1e-20 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014780)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g50440
malus_domestica MD09G1036300.v1.1 MD17G1037900.v1.1
prunus_persica Prupe.3G281400_v2.0.a1
pyrus_communis pycom111g02910
rosa_chinensis RchiOBHm_Chr2g0170861
rosa_laevigata RLG00000021987
rosa_multiflora Rmu_co8503595.1_g000001 Rmu_sc0009938.1_g000006 Rmu_sc0042424.1_g000001
rosa_roxburghii Rroxscaffold_2G00081010
rosa_rugosa Rorug02G0551800
rosa_samantha Rh2AG624600 Rh2BG635200 Rh2CG604000 Rh2DG646800
rosa_wichuraiana Rw2G051740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 163
AccII CGCG 1 cut(s) 643
AciI CCGC 3 cut(s) 402, 748, 897
AclWI GGATC 2 cut(s) 164, 1374
AcsI RAATTY 3 cut(s) 9, 351, 572
AdeI CACNNNGTG 1 cut(s) 1633
AfaI GTAC 3 cut(s) 1067, 1250, 1593
AfiI CCNNNNNNNGG 2 cut(s) 1069, 1305
AflIII ACRYGT 1 cut(s) 200
AhlI ACTAGT 1 cut(s) 59
AjnI CCWGG 3 cut(s) 136, 681, 764
AjuI GAANNNNNNNTTGG 2 cut(s) 699, 731
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 9 cut(s) 359, 382, 553, 668, 1025, 1147, 1228, 1352, 1573
AluI AGCT 9 cut(s) 359, 382, 553, 668, 1025, 1147, 1228, 1352, 1573
Alw26I GTCTC 3 cut(s) 74, 610, 660
AlwI GGATC 2 cut(s) 164, 1374
AoxI GGCC 1 cut(s) 520
ApeKI GCWGC 5 cut(s) 302, 379, 665, 1328, 1508
ApoI RAATTY 3 cut(s) 9, 351, 572
Asp700I GAANNNNTTC 1 cut(s) 1227
AspLEI GCGC 1 cut(s) 643
AspS9I GGNCC 2 cut(s) 156, 626
AsuC2I CCSGG 1 cut(s) 1494
AsuHPI GGTGA 2 cut(s) 304, 1720
AsuII TTCGAA 1 cut(s) 1409
AvaII GGWCC 2 cut(s) 156, 626
BaeGI GKGCMC 1 cut(s) 1295
BbsI GAAGAC 1 cut(s) 1818
BbvCI CCTCAGC 1 cut(s) 1148
BbvI GCAGC 5 cut(s) 289, 391, 677, 1340, 1495
BccI CCATC 2 cut(s) 1373, 1699
BcgI CGANNNNNNTGC 2 cut(s) 926, 960
BciT130I CCWGG 3 cut(s) 138, 683, 766
BclI TGATCA 1 cut(s) 1788
BcnI CCSGG 1 cut(s) 1494
BcoDI GTCTC 3 cut(s) 74, 610, 660
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 4 cut(s) 60, 387, 1311, 1758
BfmI CTRYAG 2 cut(s) 737, 1653
BisI GCNGC 5 cut(s) 303, 380, 666, 1329, 1509
BlsI GCNGC 5 cut(s) 304, 381, 667, 1330, 1510
Bme1390I CCNGG 4 cut(s) 138, 683, 766, 1494
Bme18I GGWCC 2 cut(s) 156, 626
BmgT120I GGNCC 2 cut(s) 156, 626
BmiI GGNNCC 2 cut(s) 262, 1437
BmrFI CCNGG 4 cut(s) 138, 683, 766, 1494
BmrI ACTGGG 1 cut(s) 567
BmsI GCATC 4 cut(s) 805, 848, 1026, 1143
BmuI ACTGGG 1 cut(s) 567
BpiI GAAGAC 1 cut(s) 1818
BpmI CTGGAG 3 cut(s) 964, 1198, 1734
Bpu10I CCTNAGC 1 cut(s) 1148
Bpu14I TTCGAA 1 cut(s) 1409
BpuEI CTTGAG 1 cut(s) 1589
BpuMI CCSGG 1 cut(s) 1494
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 5 cut(s) 84, 672, 764, 1285, 1493
Bsc4I CCNNNNNNNGG 2 cut(s) 1069, 1305
Bse118I RCCGGY 1 cut(s) 408
Bse1I ACTGG 2 cut(s) 562, 1512
Bse3DI GCAATG 4 cut(s) 292, 657, 949, 1335
BseBI CCWGG 3 cut(s) 138, 683, 766
BseDI CCNNGG 5 cut(s) 84, 672, 764, 1285, 1493
BseGI GGATG 5 cut(s) 79, 932, 1158, 1710, 1813
BseLI CCNNNNNNNGG 2 cut(s) 1069, 1305
BseMI GCAATG 4 cut(s) 292, 657, 949, 1335
BseMII CTCAG 4 cut(s) 446, 492, 1023, 1139
BseNI ACTGG 2 cut(s) 562, 1512
BseRI GAGGAG 5 cut(s) 949, 952, 1025, 1201, 1712
BseSI GKGCMC 1 cut(s) 1295
BseXI GCAGC 5 cut(s) 289, 391, 677, 1340, 1495
BsgI GTGCAG 1 cut(s) 1347
Bsh1236I CGCG 1 cut(s) 643
BshFI GGCC 1 cut(s) 522
BsiSI CCGG 2 cut(s) 409, 1493
BslI CCNNNNNNNGG 2 cut(s) 1069, 1305
BsmAI GTCTC 3 cut(s) 74, 610, 660
BsmI GAATGC 1 cut(s) 643
BsnI GGCC 1 cut(s) 522
Bso31I GGTCTC 1 cut(s) 74
Bsp119I TTCGAA 1 cut(s) 1409
Bsp1286I GDGCHC 1 cut(s) 1295
Bsp1407I TGTACA 2 cut(s) 1065, 1591
Bsp143I GATC 5 cut(s) 169, 1366, 1467, 1474, 1788
BspACI CCGC 3 cut(s) 402, 748, 897
BspANI GGCC 1 cut(s) 522
BspCNI CTCAG 4 cut(s) 447, 493, 1024, 1140
BspFNI CGCG 1 cut(s) 643
BspHI TCATGA 1 cut(s) 1477
BspLI GGNNCC 2 cut(s) 262, 1437
BspMAI CTGCAG 1 cut(s) 1657
BspPI GGATC 2 cut(s) 164, 1374
BspT104I TTCGAA 1 cut(s) 1409
BspTNI GGTCTC 1 cut(s) 74
BsrDI GCAATG 4 cut(s) 292, 657, 949, 1335
BsrFI RCCGGY 1 cut(s) 408
BsrGI TGTACA 2 cut(s) 1065, 1591
BsrI ACTGG 2 cut(s) 562, 1512
BssAI RCCGGY 1 cut(s) 408
BssECI CCNNGG 5 cut(s) 84, 672, 764, 1285, 1493
BssMI GATC 5 cut(s) 169, 1366, 1467, 1474, 1788
BssT1I CCWWGG 3 cut(s) 84, 672, 1285
Bst2UI CCWGG 3 cut(s) 138, 683, 766
Bst4CI ACNGT 4 cut(s) 125, 374, 715, 1745
Bst6I CTCTTC 2 cut(s) 50, 1039
BstAPI GCANNNNNTGC 1 cut(s) 1337
BstAUI TGTACA 2 cut(s) 1065, 1591
BstBI TTCGAA 1 cut(s) 1409
BstC8I GCNNGC 3 cut(s) 663, 1052, 1693
BstDEI CTNAG 4 cut(s) 455, 501, 1032, 1148
BstENI CCTNNNNNAGG 1 cut(s) 1067
BstF5I GGATG 5 cut(s) 79, 932, 1158, 1710, 1813
BstFNI CGCG 1 cut(s) 643
BstHHI GCGC 1 cut(s) 643
BstKTI GATC 5 cut(s) 172, 1369, 1470, 1477, 1791
BstMAI GTCTC 3 cut(s) 74, 610, 660
BstMBI GATC 5 cut(s) 169, 1366, 1467, 1474, 1788
BstMWI GCNNNNNNNGC 8 cut(s) 647, 815, 861, 903, 1153, 1311, 1337, 1517
BstNI CCWGG 3 cut(s) 138, 683, 766
BstNSI RCATGY 1 cut(s) 204
BstSCI CCNGG 4 cut(s) 136, 681, 764, 1492
BstSFI CTRYAG 2 cut(s) 737, 1653
BstSLI GKGCMC 1 cut(s) 1295
BstUI CGCG 1 cut(s) 643
BstV1I GCAGC 5 cut(s) 289, 391, 677, 1340, 1495
BstV2I GAAGAC 1 cut(s) 1818
BstX2I RGATCY 1 cut(s) 1366
BstXI CCANNNNNNTGG 2 cut(s) 557, 988
BstYI RGATCY 1 cut(s) 1366
BsuRI GGCC 1 cut(s) 522
BtsCI GGATG 5 cut(s) 79, 932, 1158, 1710, 1813
BtsI GCAGTG 1 cut(s) 700
BtsIMutI CAGTG 1 cut(s) 700
Cac8I GCNNGC 3 cut(s) 663, 1052, 1693
CciI TCATGA 1 cut(s) 1477
CfoI GCGC 1 cut(s) 643
Cfr10I RCCGGY 1 cut(s) 408
Cfr13I GGNCC 2 cut(s) 156, 626
Csp6I GTAC 3 cut(s) 1066, 1249, 1592
CspCI CAANNNNNGTGG 1 cut(s) 1810
CviQI GTAC 3 cut(s) 1066, 1249, 1592
DdeI CTNAG 4 cut(s) 455, 501, 1032, 1148
DpnI GATC 5 cut(s) 171, 1368, 1469, 1476, 1790
DpnII GATC 5 cut(s) 169, 1366, 1467, 1474, 1788
DraI TTTAAA 1 cut(s) 1534
DraIII CACNNNGTG 1 cut(s) 1633
DrdI GACNNNNNNGTC 1 cut(s) 163
DseDI GACNNNNNNGTC 1 cut(s) 163
Eam1104I CTCTTC 2 cut(s) 50, 1039
EarI CTCTTC 2 cut(s) 50, 1039
Eco130I CCWWGG 3 cut(s) 84, 672, 1285
Eco147I AGGCCT 1 cut(s) 522
Eco31I GGTCTC 1 cut(s) 74
Eco32I GATATC 1 cut(s) 697
Eco47I GGWCC 2 cut(s) 156, 626
EcoNI CCTNNNNNAGG 1 cut(s) 1067
EcoRII CCWGG 3 cut(s) 136, 681, 764
EcoRV GATATC 1 cut(s) 697
EcoT14I CCWWGG 3 cut(s) 84, 672, 1285
EcoT22I ATGCAT 1 cut(s) 1362
ErhI CCWWGG 3 cut(s) 84, 672, 1285
FalI AAGNNNNNCTT 2 cut(s) 1212, 1244
FbaI TGATCA 1 cut(s) 1788
Fnu4HI GCNGC 5 cut(s) 303, 380, 666, 1329, 1509
FokI GGATG 5 cut(s) 86, 919, 1165, 1717, 1820
Fsp4HI GCNGC 5 cut(s) 303, 380, 666, 1329, 1509
FspBI CTAG 4 cut(s) 60, 387, 1311, 1758
GlaI GCGC 1 cut(s) 642
GluI GCNGC 5 cut(s) 303, 380, 666, 1329, 1509
GsuI CTGGAG 3 cut(s) 964, 1198, 1734
HaeIII GGCC 1 cut(s) 522
HapII CCGG 2 cut(s) 409, 1493
HhaI GCGC 1 cut(s) 643
Hin6I GCGC 1 cut(s) 641
HinP1I GCGC 1 cut(s) 641
HincII GTYRAC 2 cut(s) 1609, 1741
HindII GTYRAC 2 cut(s) 1609, 1741
HindIII AAGCTT 1 cut(s) 1226
HinfI GANTC 6 cut(s) 89, 141, 206, 231, 431, 1561
HpaII CCGG 2 cut(s) 409, 1493
HphI GGTGA 2 cut(s) 304, 1720
Hpy166II GTNNAC 4 cut(s) 1609, 1627, 1711, 1741
Hpy188I TCNGA 4 cut(s) 169, 369, 937, 1684
Hpy188III TCNNGA 9 cut(s) 320, 417, 428, 634, 1215, 1478, 1568, 1640, 1751
Hpy8I GTNNAC 4 cut(s) 1609, 1627, 1711, 1741
HpyAV CCTTC 7 cut(s) 112, 275, 453, 607, 799, 1492, 1679
HpyCH4III ACNGT 4 cut(s) 125, 374, 715, 1745
HpyF10VI GCNNNNNNNGC 8 cut(s) 647, 815, 861, 903, 1153, 1311, 1337, 1517
HpyF3I CTNAG 4 cut(s) 455, 501, 1032, 1148
HspAI GCGC 1 cut(s) 641
Ksp22I TGATCA 1 cut(s) 1788
Kzo9I GATC 5 cut(s) 169, 1366, 1467, 1474, 1788
LmnI GCTCC 3 cut(s) 1030, 1435, 1722
Lsp1109I GCAGC 5 cut(s) 289, 391, 677, 1340, 1495
LweI GCATC 4 cut(s) 805, 848, 1026, 1143
MaeI CTAG 4 cut(s) 60, 387, 1311, 1758
MaeIII GTNAC 4 cut(s) 223, 495, 752, 922
MalI GATC 5 cut(s) 171, 1368, 1469, 1476, 1790
MboI GATC 5 cut(s) 169, 1366, 1467, 1474, 1788
MflI RGATCY 1 cut(s) 1366
MhlI GDGCHC 1 cut(s) 1295
MluCI AATT 3 cut(s) 9, 351, 572
MlyI GAGTC 1 cut(s) 225
MmeI TCCRAC 2 cut(s) 134, 633
Mph1103I ATGCAT 1 cut(s) 1362
MroXI GAANNNNTTC 1 cut(s) 1227
MseI TTAA 5 cut(s) 327, 446, 1113, 1400, 1533
MslI CAYNNNNRTG 1 cut(s) 1182
MspA1I CMGCKG 2 cut(s) 359, 748
MspI CCGG 2 cut(s) 409, 1493
MspR9I CCNGG 4 cut(s) 138, 683, 766, 1494
Mva1269I GAATGC 1 cut(s) 643
MvaI CCWGG 3 cut(s) 138, 683, 766
MvnI CGCG 1 cut(s) 643
MwoI GCNNNNNNNGC 8 cut(s) 647, 815, 861, 903, 1153, 1311, 1337, 1517
NciI CCSGG 1 cut(s) 1494
NdeII GATC 5 cut(s) 169, 1366, 1467, 1474, 1788
NlaIV GGNNCC 2 cut(s) 262, 1437
NmuCI GTSAC 1 cut(s) 223
NsiI ATGCAT 1 cut(s) 1362
NspI RCATGY 1 cut(s) 204
NspV TTCGAA 1 cut(s) 1409
PagI TCATGA 1 cut(s) 1477
PceI AGGCCT 1 cut(s) 522
PciI ACATGT 1 cut(s) 200
PctI GAATGC 1 cut(s) 643
PdmI GAANNNNTTC 1 cut(s) 1227
PfeI GAWTC 5 cut(s) 89, 141, 206, 431, 1561
PfoI TCCNGGA 1 cut(s) 681
PkrI GCNGC 5 cut(s) 304, 381, 667, 1330, 1510
PleI GAGTC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 225
PscI ACATGT 1 cut(s) 200
Psp6I CCWGG 3 cut(s) 136, 681, 764
PspGI CCWGG 3 cut(s) 136, 681, 764
PspN4I GGNNCC 2 cut(s) 262, 1437
PspPI GGNCC 2 cut(s) 156, 626
PstI CTGCAG 1 cut(s) 1657
PsuI RGATCY 1 cut(s) 1366
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 3 cut(s) 1067, 1250, 1593
RsaNI GTAC 3 cut(s) 1066, 1249, 1592
RseI CAYNNNNRTG 1 cut(s) 1182
SaqAI TTAA 5 cut(s) 327, 446, 1113, 1400, 1533
SatI GCNGC 5 cut(s) 303, 380, 666, 1329, 1509
Sau3AI GATC 5 cut(s) 169, 1366, 1467, 1474, 1788
Sau96I GGNCC 2 cut(s) 156, 626
SchI GAGTC 1 cut(s) 225
ScrFI CCNGG 4 cut(s) 138, 683, 766, 1494
SduI GDGCHC 1 cut(s) 1295
SfaNI GCATC 4 cut(s) 805, 848, 1026, 1143
SfcI CTRYAG 2 cut(s) 737, 1653
SfuI TTCGAA 1 cut(s) 1409
SinI GGWCC 2 cut(s) 156, 626
SmiMI CAYNNNNRTG 1 cut(s) 1182
SmlI CTYRAG 1 cut(s) 1568
SmoI CTYRAG 1 cut(s) 1568
SpeI ACTAGT 1 cut(s) 59
Sse9I AATT 3 cut(s) 9, 351, 572
SseBI AGGCCT 1 cut(s) 522
SsiI CCGC 3 cut(s) 402, 748, 897
SspMI CTAG 4 cut(s) 60, 387, 1311, 1758
StuI AGGCCT 1 cut(s) 522
StyD4I CCNGG 4 cut(s) 136, 681, 764, 1492
StyI CCWWGG 3 cut(s) 84, 672, 1285
TaaI ACNGT 4 cut(s) 125, 374, 715, 1745
TaqI TCGA 4 cut(s) 881, 1130, 1409, 1639
TaqII GACCGA 1 cut(s) 1777
TasI AATT 3 cut(s) 9, 351, 572
TatI WGTACW 3 cut(s) 1065, 1248, 1591
TfiI GAWTC 5 cut(s) 89, 141, 206, 431, 1561
Tru1I TTAA 5 cut(s) 327, 446, 1113, 1400, 1533
Tru9I TTAA 5 cut(s) 327, 446, 1113, 1400, 1533
TscAI CASTG 1 cut(s) 707
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 5 cut(s) 302, 379, 665, 1328, 1508
Tsp45I GTSAC 1 cut(s) 223
TspGWI ACGGA 3 cut(s) 4, 1293, 1617
TspRI CASTG 1 cut(s) 707
VpaK11BI GGWCC 2 cut(s) 156, 626
XagI CCTNNNNNAGG 1 cut(s) 1067
XapI RAATTY 3 cut(s) 9, 351, 572
XceI RCATGY 1 cut(s) 204
XmnI GAANNNNTTC 1 cut(s) 1227
XspI CTAG 4 cut(s) 60, 387, 1311, 1758
Zsp2I ATGCAT 1 cut(s) 1362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.