Rroxscaffold_2G00105740

Belongs to the spermidine spermine synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0020765
Physical Location & Seq
Forward (+)
29167517 .. 29174677
7161 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00105740.1

Sequence Viewer

Length: 294 bp
ATGGGGGAAGACGCTGGAAGAGGTTTGGAATGCCAGAAGACTATGGATGGGAAGGTGAGTAACATAAACGGTTCAGAGAAGGCCATTCCTTCTTGTTGCTTGAAGGCTAGGGCTTCAGATCCTGAGGCTGATGCAATAAGCCATTCTACTGTTGTTTCTGGGTGGTTTTCAGAATCCCAGTCTCGCACAGATCTTCAAGTTGAAACCTGCTGCAAGGGCCCATTAGCTCACTTTTTCTTTAGAAAATTCAACATTCCAGGTCAACGTGCATCAACAGTGGTAGCAAGGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

97

Amino Acids

10.44

Weight (kDa)

7.64

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020765)

Species Orthologous Gene IDs
rosa_roxburghii Rroxscaffold_2G00105740
rosa_rugosa Rorug01G0065800 Rorug02G0365500 Rorug06G0145800
rosa_samantha Rh3BG357200
rosa_wichuraiana Rw0G005130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 215
AclWI GGATC 1 cut(s) 113
AcsI RAATTY 1 cut(s) 245
AcuI CTGAAG 1 cut(s) 99
AgsI TTSAA 4 cut(s) 103, 197, 203, 250
AjnI CCWGG 1 cut(s) 256
AluBI AGCT 1 cut(s) 227
AluI AGCT 1 cut(s) 227
Alw26I GTCTC 1 cut(s) 186
AlwI GGATC 1 cut(s) 113
AlwNI CAGNNNCTG 1 cut(s) 122
AoxI GGCC 2 cut(s) 81, 217
ApaI GGGCCC 1 cut(s) 221
ApeKI GCWGC 1 cut(s) 210
ApoI RAATTY 1 cut(s) 245
AspS9I GGNCC 2 cut(s) 217, 218
AsuHPI GGTGA 1 cut(s) 67
AxyI CCTNAGG 1 cut(s) 123
BaeGI GKGCMC 1 cut(s) 221
BanII GRGCYC 1 cut(s) 221
BbsI GAAGAC 2 cut(s) 15, 44
BbvI GCAGC 1 cut(s) 197
BccI CCATC 1 cut(s) 41
BciT130I CCWGG 1 cut(s) 258
BcoDI GTCTC 1 cut(s) 186
BfaI CTAG 1 cut(s) 108
BfuAI ACCTGC 1 cut(s) 215
BglII AGATCT 1 cut(s) 190
BisI GCNGC 1 cut(s) 211
BlsI GCNGC 1 cut(s) 212
Bme1390I CCNGG 1 cut(s) 258
BmgT120I GGNCC 2 cut(s) 217, 218
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 1 cut(s) 258
BmrI ACTGGG 1 cut(s) 172
BmsI GCATC 2 cut(s) 121, 278
BmuI ACTGGG 1 cut(s) 172
BpiI GAAGAC 2 cut(s) 15, 44
Bse1I ACTGG 1 cut(s) 178
Bse21I CCTNAGG 1 cut(s) 123
BseBI CCWGG 1 cut(s) 258
BseGI GGATG 1 cut(s) 52
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 1 cut(s) 178
BseSI GKGCMC 1 cut(s) 221
BseXI GCAGC 1 cut(s) 197
BshFI GGCC 2 cut(s) 83, 219
BsmAI GTCTC 1 cut(s) 186
BsmI GAATGC 1 cut(s) 35
BsnI GGCC 2 cut(s) 83, 219
Bsp120I GGGCCC 1 cut(s) 217
Bsp1286I GDGCHC 1 cut(s) 221
Bsp143I GATC 2 cut(s) 118, 190
BspANI GGCC 2 cut(s) 83, 219
BspCNI CTCAG 1 cut(s) 115
BspLI GGNNCC 1 cut(s) 219
BspMI ACCTGC 1 cut(s) 215
BspPI GGATC 1 cut(s) 113
BsrI ACTGG 1 cut(s) 178
BssMI GATC 2 cut(s) 118, 190
Bst2UI CCWGG 1 cut(s) 258
Bst4CI ACNGT 3 cut(s) 71, 151, 277
Bst6I CTCTTC 1 cut(s) 13
BstDEI CTNAG 1 cut(s) 123
BstF5I GGATG 1 cut(s) 52
BstKTI GATC 2 cut(s) 121, 193
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 2 cut(s) 118, 190
BstMWI GCNNNNNNNGC 1 cut(s) 216
BstNI CCWGG 1 cut(s) 258
BstSCI CCNGG 1 cut(s) 256
BstSLI GKGCMC 1 cut(s) 221
BstV1I GCAGC 1 cut(s) 197
BstV2I GAAGAC 2 cut(s) 15, 44
BstX2I RGATCY 2 cut(s) 118, 190
BstYI RGATCY 2 cut(s) 118, 190
Bsu36I CCTNAGG 1 cut(s) 123
BsuRI GGCC 2 cut(s) 83, 219
BtsCI GGATG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 282
BveI ACCTGC 1 cut(s) 215
CaiI CAGNNNCTG 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 217, 218
CseI GACGC 1 cut(s) 20
CviJI RGCY 7 cut(s) 83, 107, 113, 128, 141, 219, 227
CviKI_1 RGCY 7 cut(s) 83, 107, 113, 128, 141, 219, 227
DdeI CTNAG 1 cut(s) 123
DpnI GATC 2 cut(s) 120, 192
DpnII GATC 2 cut(s) 118, 190
Eam1104I CTCTTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 13
Eco24I GRGCYC 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 99
Eco81I CCTNAGG 1 cut(s) 123
EcoO109I RGGNCCY 1 cut(s) 217
EcoRII CCWGG 1 cut(s) 256
EcoT38I GRGCYC 1 cut(s) 221
FaiI YATR 2 cut(s) 44, 65
Fnu4HI GCNGC 1 cut(s) 211
FokI GGATG 1 cut(s) 59
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 1 cut(s) 211
FspBI CTAG 1 cut(s) 108
GluI GCNGC 1 cut(s) 211
HaeIII GGCC 2 cut(s) 83, 219
HgaI GACGC 1 cut(s) 20
HincII GTYRAC 1 cut(s) 263
HindII GTYRAC 1 cut(s) 263
HinfI GANTC 1 cut(s) 173
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 1 cut(s) 263
Hpy188I TCNGA 3 cut(s) 76, 118, 172
Hpy188III TCNNGA 1 cut(s) 122
Hpy8I GTNNAC 1 cut(s) 263
HpyAV CCTTC 4 cut(s) 46, 73, 97, 99
HpyCH4III ACNGT 3 cut(s) 71, 151, 277
HpyCH4IV ACGT 1 cut(s) 265
HpyCH4V TGCA 3 cut(s) 134, 213, 269
HpyF10VI GCNNNNNNNGC 1 cut(s) 216
HpyF3I CTNAG 1 cut(s) 123
HpySE526I ACGT 1 cut(s) 265
Kzo9I GATC 2 cut(s) 118, 190
LpnPI CCDG 7 cut(s) 47, 135, 144, 191, 220, 243, 270
Lsp1109I GCAGC 1 cut(s) 197
LweI GCATC 2 cut(s) 121, 278
MaeI CTAG 1 cut(s) 108
MaeII ACGT 1 cut(s) 265
MaeIII GTNAC 1 cut(s) 59
MalI GATC 2 cut(s) 120, 192
MboI GATC 2 cut(s) 118, 190
MboII GAAGA 4 cut(s) 20, 30, 49, 185
MflI RGATCY 2 cut(s) 118, 190
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 1 cut(s) 245
MnlI CCTC 2 cut(s) 14, 118
MspR9I CCNGG 1 cut(s) 258
Mva1269I GAATGC 1 cut(s) 35
MvaI CCWGG 1 cut(s) 258
MwoI GCNNNNNNNGC 1 cut(s) 216
NdeII GATC 2 cut(s) 118, 190
NlaIV GGNNCC 1 cut(s) 219
PctI GAATGC 1 cut(s) 35
PfeI GAWTC 1 cut(s) 173
PkrI GCNGC 1 cut(s) 212
Psp6I CCWGG 1 cut(s) 256
PspGI CCWGG 1 cut(s) 256
PspN4I GGNNCC 1 cut(s) 219
PspOMI GGGCCC 1 cut(s) 217
PspPI GGNCC 2 cut(s) 217, 218
PstNI CAGNNNCTG 1 cut(s) 122
PsuI RGATCY 2 cut(s) 118, 190
SatI GCNGC 1 cut(s) 211
Sau3AI GATC 2 cut(s) 118, 190
Sau96I GGNCC 2 cut(s) 217, 218
ScrFI CCNGG 1 cut(s) 258
SduI GDGCHC 1 cut(s) 221
SetI ASST 7 cut(s) 25, 57, 209, 229, 262, 268, 290
SfaNI GCATC 2 cut(s) 121, 278
Sse9I AATT 1 cut(s) 245
SspMI CTAG 1 cut(s) 108
StyD4I CCNGG 1 cut(s) 256
TaaI ACNGT 3 cut(s) 71, 151, 277
TaiI ACGT 1 cut(s) 268
TasI AATT 1 cut(s) 245
TfiI GAWTC 1 cut(s) 173
TscAI CASTG 1 cut(s) 282
TseI GCWGC 1 cut(s) 210
TspRI CASTG 1 cut(s) 282
XapI RAATTY 1 cut(s) 245
XspI CTAG 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.