Rroxscaffold_2G00127880

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0020417
Physical Location & Seq
Forward (+)
63448650 .. 63450210
1561 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_2G00127880.1

Sequence Viewer

Length: 318 bp
ATGCGACTCCTTCAAGGCTTCAATCCCAGATCCCAGATAACTCAAGCTCATATGCAAGAACAAGTCGAGGAGCTGAAGCAAGTAATGAGAAAGGAAGTATTCACTGATGATGCTGATGATTTTTTACATCAACTGAAGCTAATTGATTCAATCCAGCGCCTCGGTGTGGCTTACCATTTTGAAATTGAAATAGAACAAGCCCCGAAACTTATACATGCTAACTATCAAGATATTGATGATGATGATAGTGATCTGTACAATGTTGCTCTTCGTTTTCGACTACTCGGACGACATGGATATAATGTTTCTTGCGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

105

Amino Acids

12.3

Weight (kDa)

5.03

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 11 - 104 1e-24 Terpene synthase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020417)

Species Orthologous Gene IDs
prunus_persica Prupe.4G193900_v2.0.a1
rosa_multiflora Rmu_co8170300.1_g000001
rosa_roxburghii Rroxscaffold_2G00127880 Rroxscaffold_2G00128760
rosa_samantha Rh3DG216400 Rh3DG328500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 312
AclWI GGATC 1 cut(s) 24
AcuI CTGAAG 2 cut(s) 95, 155
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 1 cut(s) 166
AgsI TTSAA 5 cut(s) 14, 22, 150, 182, 188
AluBI AGCT 3 cut(s) 47, 73, 139
AluI AGCT 3 cut(s) 47, 73, 139
AlwI GGATC 1 cut(s) 24
AspLEI GCGC 1 cut(s) 159
BfoI RGCGCY 1 cut(s) 160
BmsI GCATC 1 cut(s) 100
BpuEI CTTGAG 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 249
BsaJI CCNNGG 1 cut(s) 160
Bsc4I CCNNNNNNNGG 1 cut(s) 166
Bse8I GATNNNNATC 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 160
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 1 cut(s) 166
BseRI GAGGAG 1 cut(s) 83
BslI CCNNNNNNNGG 1 cut(s) 166
Bsp1407I TGTACA 1 cut(s) 255
Bsp143I GATC 2 cut(s) 29, 250
BspACI CCGC 1 cut(s) 312
BspPI GGATC 1 cut(s) 24
BspQI GCTCTTC 1 cut(s) 273
BsrGI TGTACA 1 cut(s) 255
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 2 cut(s) 29, 250
Bst6I CTCTTC 1 cut(s) 273
BstAUI TGTACA 1 cut(s) 255
BstH2I RGCGCY 1 cut(s) 160
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 2 cut(s) 32, 253
BstMBI GATC 2 cut(s) 29, 250
BstNSI RCATGY 1 cut(s) 218
BstX2I RGATCY 1 cut(s) 29
BstYI RGATCY 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 102
CfoI GCGC 1 cut(s) 159
Csp6I GTAC 1 cut(s) 256
CviAII CATG 2 cut(s) 215, 293
CviJI RGCY 6 cut(s) 18, 47, 73, 139, 170, 200
CviKI_1 RGCY 6 cut(s) 18, 47, 73, 139, 170, 200
CviQI GTAC 1 cut(s) 256
DpnI GATC 2 cut(s) 31, 252
DpnII GATC 2 cut(s) 29, 250
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
Eco57I CTGAAG 2 cut(s) 95, 155
FaeI CATG 2 cut(s) 218, 296
FaiI YATR 6 cut(s) 51, 53, 212, 216, 294, 300
FatI CATG 2 cut(s) 214, 292
FauNDI CATATG 1 cut(s) 51
GlaI GCGC 1 cut(s) 158
HaeII RGCGCY 1 cut(s) 160
HhaI GCGC 1 cut(s) 159
Hin1II CATG 2 cut(s) 218, 296
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HinfI GANTC 2 cut(s) 6, 146
Hpy188I TCNGA 1 cut(s) 287
Hpy188III TCNNGA 1 cut(s) 227
HpyAV CCTTC 1 cut(s) 20
HpyCH4V TGCA 1 cut(s) 55
Hsp92II CATG 2 cut(s) 218, 296
HspAI GCGC 1 cut(s) 157
Kzo9I GATC 2 cut(s) 29, 250
LguI GCTCTTC 1 cut(s) 273
LmnI GCTCC 1 cut(s) 70
LpnPI CCDG 3 cut(s) 40, 47, 167
LweI GCATC 1 cut(s) 100
MalI GATC 2 cut(s) 31, 252
MboI GATC 2 cut(s) 29, 250
MboII GAAGA 1 cut(s) 260
MflI RGATCY 1 cut(s) 29
MluCI AATT 2 cut(s) 141, 183
MnlI CCTC 2 cut(s) 61, 170
NdeI CATATG 1 cut(s) 51
NdeII GATC 2 cut(s) 29, 250
NlaIII CATG 2 cut(s) 218, 296
NspI RCATGY 1 cut(s) 218
PciSI GCTCTTC 1 cut(s) 273
PfeI GAWTC 1 cut(s) 146
PsuI RGATCY 1 cut(s) 29
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SapI GCTCTTC 1 cut(s) 273
Sau3AI GATC 2 cut(s) 29, 250
SetI ASST 3 cut(s) 49, 75, 141
SfaNI GCATC 1 cut(s) 100
SmlI CTYRAG 1 cut(s) 42
SmoI CTYRAG 1 cut(s) 42
Sse9I AATT 2 cut(s) 141, 183
SsiI CCGC 1 cut(s) 312
TaqI TCGA 2 cut(s) 66, 277
TasI AATT 2 cut(s) 141, 183
TatI WGTACW 1 cut(s) 255
TfiI GAWTC 1 cut(s) 146
TscAI CASTG 1 cut(s) 109
TspRI CASTG 1 cut(s) 109
XceI RCATGY 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.