Rh3DG328500

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0020417
Physical Location & Seq
Reverse (-)
36110259 .. 36111164
906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG328500.1

Sequence Viewer

Length: 588 bp
ATGTCGTCTATGCCAATTATTTCTGCATCATTTCAAGTACCGCAAATTTCAAAGCTTGAAATTGTTCGCCGGACGGCAAATTTTCCACCAAGCATATGGGGAGATCGCTTCATCAACTATGATTCCCAACACACTCAGAAGACTGATGCCTTTTGGCCGCAACAGGTTGAAAAACTGAAAACAGTAGTGAAGAGGGAAGTTTTTACTAATGAATGTGATTTTTCACATCGACTCAAGTTCATTGATGCAATACAACGACTTGGCGTGGCATACCAATTTGAACATGAAACAGAAGAATCACTGCAACATATCCATGCTAAATATTGTGATCATGATCAGTTTCGTGACGACAACCATCTTTACAATGTTGCGCTATGTTTTCGGCTTCTAAGGCAACATGGATATCATATTTCATCTAATATATTCAACAAGTTCAAAGATTCAAATGTTAGCTTCAAAAAATGCGTAATTGCTGACGCCTATGGTATGCTAAGCCTCTATGAAGCGACACATCTTAGGGTTAATGGAGAAGATATACTTGAAGCTCTTCTGTTCACCGGCCACCACTCGACTAGAGTCTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

195

Amino Acids

22.75

Weight (kDa)

6.86

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 32 - 189 8.3e-47 Terpene synthase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020417)

Species Orthologous Gene IDs
prunus_persica Prupe.4G193900_v2.0.a1
rosa_multiflora Rmu_co8170300.1_g000001
rosa_roxburghii Rroxscaffold_2G00127880 Rroxscaffold_2G00128760
rosa_samantha Rh3DG216400 Rh3DG328500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 41, 158
AcoI YGGCCR 2 cut(s) 155, 559
AcsI RAATTY 2 cut(s) 45, 79
AcyI GRCGYC 1 cut(s) 477
AfaI GTAC 1 cut(s) 39
AluBI AGCT 3 cut(s) 55, 453, 545
AluI AGCT 3 cut(s) 55, 453, 545
Alw26I GTCTC 1 cut(s) 583
AoxI GGCC 2 cut(s) 155, 559
ApoI RAATTY 2 cut(s) 45, 79
Asp700I GAANNNNTTC 2 cut(s) 63, 546
AspLEI GCGC 1 cut(s) 373
AsuHPI GGTGA 1 cut(s) 547
BbsI GAAGAC 1 cut(s) 146
BccI CCATC 1 cut(s) 363
BceAI ACGGC 1 cut(s) 90
BclI TGATCA 2 cut(s) 328, 334
BcoDI GTCTC 1 cut(s) 583
BfaI CTAG 1 cut(s) 573
BisI GCNGC 1 cut(s) 158
BlpI GCTNAGC 1 cut(s) 491
BlsI GCNGC 1 cut(s) 159
BmsI GCATC 3 cut(s) 35, 136, 235
BoxI GACNNNNGTC 1 cut(s) 575
BpiI GAAGAC 1 cut(s) 146
Bpu1102I GCTNAGC 1 cut(s) 491
BpuEI CTTGAG 1 cut(s) 218
BsaBI GATNNNNATC 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 477
Bse118I RCCGGY 1 cut(s) 557
Bse8I GATNNNNATC 1 cut(s) 333
BseJI GATNNNNATC 1 cut(s) 333
BseMII CTCAG 1 cut(s) 149
BshFI GGCC 2 cut(s) 157, 561
BsiSI CCGG 2 cut(s) 70, 558
BsmAI GTCTC 1 cut(s) 583
BsnI GGCC 2 cut(s) 157, 561
Bsp143I GATC 3 cut(s) 103, 328, 334
Bsp1720I GCTNAGC 1 cut(s) 491
BspACI CCGC 2 cut(s) 41, 158
BspANI GGCC 2 cut(s) 157, 561
BspCNI CTCAG 1 cut(s) 148
BspHI TCATGA 1 cut(s) 331
BspQI GCTCTTC 1 cut(s) 552
BsrFI RCCGGY 1 cut(s) 557
BssAI RCCGGY 1 cut(s) 557
BssMI GATC 3 cut(s) 103, 328, 334
BssNI GRCGYC 1 cut(s) 477
Bst4CI ACNGT 1 cut(s) 184
Bst6I CTCTTC 2 cut(s) 185, 552
BstACI GRCGYC 1 cut(s) 477
BstDEI CTNAG 4 cut(s) 135, 389, 491, 515
BstHHI GCGC 1 cut(s) 373
BstKTI GATC 3 cut(s) 106, 331, 337
BstMAI GTCTC 1 cut(s) 583
BstMBI GATC 3 cut(s) 103, 328, 334
BstMWI GCNNNNNNNGC 1 cut(s) 391
BstPAI GACNNNNGTC 1 cut(s) 575
BstV2I GAAGAC 1 cut(s) 146
BstXI CCANNNNNNTGG 1 cut(s) 96
BsuRI GGCC 2 cut(s) 157, 561
BtsI GCAGTG 1 cut(s) 299
BtsIMutI CAGTG 1 cut(s) 299
CciI TCATGA 1 cut(s) 331
CfoI GCGC 1 cut(s) 373
Cfr10I RCCGGY 1 cut(s) 557
CseI GACGC 1 cut(s) 485
Csp6I GTAC 1 cut(s) 38
CviAII CATG 4 cut(s) 284, 314, 332, 398
CviJI RGCY 7 cut(s) 55, 157, 385, 453, 495, 545, 561
CviKI_1 RGCY 7 cut(s) 55, 157, 385, 453, 495, 545, 561
CviQI GTAC 1 cut(s) 38
DdeI CTNAG 4 cut(s) 135, 389, 491, 515
DpnI GATC 3 cut(s) 105, 330, 336
DpnII GATC 3 cut(s) 103, 328, 334
EaeI YGGCCR 2 cut(s) 155, 559
Eam1104I CTCTTC 2 cut(s) 185, 552
EarI CTCTTC 2 cut(s) 185, 552
Eco32I GATATC 1 cut(s) 404
EcoRV GATATC 1 cut(s) 404
FaeI CATG 4 cut(s) 287, 317, 335, 401
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FatI CATG 4 cut(s) 283, 313, 331, 397
FauNDI CATATG 1 cut(s) 95
FbaI TGATCA 2 cut(s) 328, 334
Fnu4HI GCNGC 1 cut(s) 158
Fsp4HI GCNGC 1 cut(s) 158
FspBI CTAG 1 cut(s) 573
GlaI GCGC 1 cut(s) 372
GluI GCNGC 1 cut(s) 158
HaeIII GGCC 2 cut(s) 157, 561
HapII CCGG 2 cut(s) 70, 558
HgaI GACGC 1 cut(s) 485
HhaI GCGC 1 cut(s) 373
Hin1I GRCGYC 1 cut(s) 477
Hin1II CATG 4 cut(s) 287, 317, 335, 401
Hin6I GCGC 1 cut(s) 371
HinP1I GCGC 1 cut(s) 371
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 5 cut(s) 122, 231, 296, 440, 576
HpaII CCGG 2 cut(s) 70, 558
HphI GGTGA 1 cut(s) 547
Hpy166II GTNNAC 1 cut(s) 555
Hpy188I TCNGA 1 cut(s) 138
Hpy188III TCNNGA 2 cut(s) 332, 344
Hpy8I GTNNAC 1 cut(s) 555
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 3 cut(s) 26, 248, 304
HpyF10VI GCNNNNNNNGC 1 cut(s) 391
HpyF3I CTNAG 4 cut(s) 135, 389, 491, 515
Hsp92I GRCGYC 1 cut(s) 477
Hsp92II CATG 4 cut(s) 287, 317, 335, 401
HspAI GCGC 1 cut(s) 371
Ksp22I TGATCA 2 cut(s) 328, 334
Kzo9I GATC 3 cut(s) 103, 328, 334
LguI GCTCTTC 1 cut(s) 552
LpnPI CCDG 3 cut(s) 83, 149, 571
LweI GCATC 3 cut(s) 35, 136, 235
MaeI CTAG 1 cut(s) 573
MaeIII GTNAC 1 cut(s) 344
MalI GATC 3 cut(s) 105, 330, 336
MboI GATC 3 cut(s) 103, 328, 334
MboII GAAGA 5 cut(s) 151, 202, 305, 539, 542
MluCI AATT 6 cut(s) 15, 45, 60, 79, 275, 468
MlyI GAGTC 2 cut(s) 225, 585
MnlI CCTC 2 cut(s) 186, 506
MroXI GAANNNNTTC 2 cut(s) 63, 546
MseI TTAA 2 cut(s) 522, 586
MslI CAYNNNNRTG 1 cut(s) 312
MspI CCGG 2 cut(s) 70, 558
MwoI GCNNNNNNNGC 1 cut(s) 391
NdeI CATATG 1 cut(s) 95
NdeII GATC 3 cut(s) 103, 328, 334
NlaIII CATG 4 cut(s) 287, 317, 335, 401
NmuCI GTSAC 1 cut(s) 344
PagI TCATGA 1 cut(s) 331
PciSI GCTCTTC 1 cut(s) 552
PdmI GAANNNNTTC 2 cut(s) 63, 546
PfeI GAWTC 3 cut(s) 122, 296, 440
PkrI GCNGC 1 cut(s) 159
PleI GAGTC 2 cut(s) 225, 584
PpsI GAGTC 2 cut(s) 225, 584
PshAI GACNNNNGTC 1 cut(s) 575
RsaI GTAC 1 cut(s) 39
RsaNI GTAC 1 cut(s) 38
RseI CAYNNNNRTG 1 cut(s) 312
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 2 cut(s) 522, 586
SatI GCNGC 1 cut(s) 158
Sau3AI GATC 3 cut(s) 103, 328, 334
SchI GAGTC 2 cut(s) 225, 585
SetI ASST 4 cut(s) 57, 168, 455, 547
SfaNI GCATC 3 cut(s) 35, 136, 235
SmiMI CAYNNNNRTG 1 cut(s) 312
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
Sse9I AATT 6 cut(s) 15, 45, 60, 79, 275, 468
SsiI CCGC 2 cut(s) 41, 158
SspI AATATT 1 cut(s) 323
SspMI CTAG 1 cut(s) 573
TaaI ACNGT 1 cut(s) 184
TaqI TCGA 2 cut(s) 229, 569
TasI AATT 6 cut(s) 15, 45, 60, 79, 275, 468
TauI GCSGC 1 cut(s) 160
TfiI GAWTC 3 cut(s) 122, 296, 440
Tru1I TTAA 2 cut(s) 522, 586
Tru9I TTAA 2 cut(s) 522, 586
TscAI CASTG 1 cut(s) 306
TseFI GTSAC 1 cut(s) 344
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 6 cut(s) 100, 225, 229, 300, 402, 516
TspRI CASTG 1 cut(s) 306
XapI RAATTY 2 cut(s) 45, 79
XcmI CCANNNNNNNNNTGG 1 cut(s) 93
XmnI GAANNNNTTC 2 cut(s) 63, 546
XspI CTAG 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.