Rh3DG216400

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0020417
Physical Location & Seq
Forward (+)
19966051 .. 19971525
5475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG216400.1

Sequence Viewer

Length: 825 bp
ATGCCTGTCCAAACTATTCCAGCAGCTGCATCCCAGATCAATGCTATGCCAGAAATTGTTCGGCGCACAGCAAATTTTAAACCTAGCGTTTGGGGAGATCGGTTTGCTAACTATGCTGAAGACATTGTAACTCAAGCTCATATGCAAAAACAAGTTGAGGAGCTGAAACAAGTAGTGAGGAAGGAAGTATTCACTGATGCTGCTGATGATTTTTCACATCAGCTGAAGCTAATTGATGCAATCCAGCGCCTTGGTGTGGCTTACCATTTTGAAACTGAAATAGAACAAGCCCTGGAACGTATACATACCTATCAAGATATTAATGATGATGATGGTGATCTGTACAATGTTGCTCTTCGTTTTCGACTACTCAGACGACATGGATATAATGTTTCCTGCGATGTATTCAACAAATTCAAAGATGTTAATGGTGACTTCAAGGAAAGCTTAGTTACTGATGTTTCTGGTATGCTAAGCTTCTATGAGGCAGCCCATCTAATGGTGCATGGAGAAAAATTACTAGAAGAGGCTCTCGTTTTTACCACCACTCATCTCGAGACTGCAACAACTTTAACCGGTGCAAGCTCTCTGCTGAAAGAACAAATAACTGAAGCCCTAGAGAGACCTCTACTAAAAACTGTGGAGAGGTTAGGTGCTCGGCGTTACATGTCAATATACCAAGATGAAGCTTCACACAATAAAGAGTTACTGAAACTTGCCAAGTTAGATTTCAATGTTGTTCAGTGTTCACACAAAAAGGAACTCAGTGACATTATAAGGTTAATGAGCCTCATATCTATAAATCTTCAGCTTCATAACCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

274

Amino Acids

31.33

Weight (kDa)

5.67

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 30 - 206 3.6e-59 Terpene synthase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020417)

Species Orthologous Gene IDs
prunus_persica Prupe.4G193900_v2.0.a1
rosa_multiflora Rmu_co8170300.1_g000001
rosa_roxburghii Rroxscaffold_2G00127880 Rroxscaffold_2G00128760
rosa_samantha Rh3DG216400 Rh3DG328500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 776
AccB7I CCANNNNNTGG 1 cut(s) 499
AccI GTMKAC 1 cut(s) 301
AcsI RAATTY 2 cut(s) 73, 413
AcuI CTGAAG 4 cut(s) 138, 245, 630, 791
AfaI GTAC 1 cut(s) 344
AfiI CCNNNNNNNGG 2 cut(s) 256, 499
AflIII ACRYGT 1 cut(s) 666
AgeI ACCGGT 1 cut(s) 575
AgsI TTSAA 5 cut(s) 272, 409, 418, 439, 733
AjnI CCWGG 1 cut(s) 291
AjuI GAANNNNNNNTTGG 2 cut(s) 713, 745
Alw21I GWGCWC 1 cut(s) 658
Alw26I GTCTC 2 cut(s) 551, 616
AlwNI CAGNNNCTG 1 cut(s) 26
Ama87I CYCGRG 1 cut(s) 554
ApeKI GCWGC 4 cut(s) 23, 26, 200, 488
ApoI RAATTY 2 cut(s) 73, 413
AseI ATTAAT 1 cut(s) 321
AsiGI ACCGGT 1 cut(s) 575
Asp700I GAANNNNTTC 1 cut(s) 57
AspLEI GCGC 2 cut(s) 66, 249
AsuHPI GGTGA 2 cut(s) 347, 443
AvaI CYCGRG 1 cut(s) 554
BbsI GAAGAC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 658
BbvI GCAGC 4 cut(s) 13, 35, 187, 500
BccI CCATC 2 cut(s) 326, 501
BciT130I CCWGG 1 cut(s) 293
BcoDI GTCTC 2 cut(s) 551, 616
BfaI CTAG 3 cut(s) 84, 521, 617
BfoI RGCGCY 1 cut(s) 250
BisI GCNGC 4 cut(s) 24, 27, 201, 489
BlpI GCTNAGC 1 cut(s) 473
BlsI GCNGC 4 cut(s) 25, 28, 202, 490
Bme1390I CCNGG 1 cut(s) 293
BmeT110I CYCGRG 1 cut(s) 554
BmrFI CCNGG 1 cut(s) 293
BmsI GCATC 3 cut(s) 38, 187, 226
BpiI GAAGAC 1 cut(s) 126
Bpu1102I GCTNAGC 1 cut(s) 473
BpuEI CTTGAG 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 616
BsaJI CCNNGG 2 cut(s) 250, 291
BsaWI WCCGGW 1 cut(s) 575
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 499
Bse118I RCCGGY 1 cut(s) 575
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 1 cut(s) 293
BseDI CCNNGG 2 cut(s) 250, 291
BseGI GGATG 1 cut(s) 29
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 2 cut(s) 256, 499
BseMII CTCAG 2 cut(s) 385, 778
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 4 cut(s) 13, 35, 187, 500
BshTI ACCGGT 1 cut(s) 575
BsiHKAI GWGCWC 1 cut(s) 658
BsiHKCI CYCGRG 1 cut(s) 554
BsiSI CCGG 1 cut(s) 576
BslI CCNNNNNNNGG 2 cut(s) 256, 499
BsmAI GTCTC 2 cut(s) 551, 616
Bso31I GGTCTC 1 cut(s) 616
BsoBI CYCGRG 1 cut(s) 554
Bsp1286I GDGCHC 1 cut(s) 658
Bsp1407I TGTACA 1 cut(s) 342
Bsp143I GATC 3 cut(s) 36, 97, 337
Bsp1720I GCTNAGC 1 cut(s) 473
BspCNI CTCAG 2 cut(s) 384, 777
BspQI GCTCTTC 1 cut(s) 360
BspTNI GGTCTC 1 cut(s) 616
BsrFI RCCGGY 1 cut(s) 575
BsrGI TGTACA 1 cut(s) 342
BssAI RCCGGY 1 cut(s) 575
BssECI CCNNGG 2 cut(s) 250, 291
BssMI GATC 3 cut(s) 36, 97, 337
BssNAI GTATAC 1 cut(s) 302
BssT1I CCWWGG 1 cut(s) 250
Bst1107I GTATAC 1 cut(s) 302
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 1 cut(s) 640
Bst6I CTCTTC 2 cut(s) 360, 519
BstAUI TGTACA 1 cut(s) 342
BstC8I GCNNGC 1 cut(s) 583
BstDEI CTNAG 5 cut(s) 371, 448, 473, 764, 822
BstF5I GGATG 1 cut(s) 29
BstH2I RGCGCY 1 cut(s) 250
BstHHI GCGC 2 cut(s) 66, 249
BstKTI GATC 3 cut(s) 39, 100, 340
BstMAI GTCTC 2 cut(s) 551, 616
BstMBI GATC 3 cut(s) 36, 97, 337
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstNI CCWGG 1 cut(s) 293
BstNSI RCATGY 1 cut(s) 670
BstSCI CCNGG 1 cut(s) 291
BstV1I GCAGC 4 cut(s) 13, 35, 187, 500
BstV2I GAAGAC 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 251
BstZ17I GTATAC 1 cut(s) 302
BtgZI GCGATG 1 cut(s) 414
BtsCI GGATG 1 cut(s) 29
BtsIMutI CAGTG 3 cut(s) 192, 749, 772
Cac8I GCNNGC 1 cut(s) 583
CaiI CAGNNNCTG 1 cut(s) 26
CfoI GCGC 2 cut(s) 66, 249
Cfr10I RCCGGY 1 cut(s) 575
Csp6I GTAC 1 cut(s) 343
CspAI ACCGGT 1 cut(s) 575
CviAII CATG 3 cut(s) 380, 506, 667
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 5 cut(s) 371, 448, 473, 764, 822
DpnI GATC 3 cut(s) 38, 99, 339
DpnII GATC 3 cut(s) 36, 97, 337
DraI TTTAAA 1 cut(s) 79
Eam1104I CTCTTC 2 cut(s) 360, 519
EarI CTCTTC 2 cut(s) 360, 519
Eco130I CCWWGG 1 cut(s) 250
Eco31I GGTCTC 1 cut(s) 616
Eco57I CTGAAG 4 cut(s) 138, 245, 630, 791
Eco88I CYCGRG 1 cut(s) 554
EcoRII CCWGG 1 cut(s) 291
EcoT14I CCWWGG 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 250
FaeI CATG 3 cut(s) 383, 509, 670
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FatI CATG 3 cut(s) 379, 505, 666
FauNDI CATATG 1 cut(s) 141
FblI GTMKAC 1 cut(s) 301
Fnu4HI GCNGC 4 cut(s) 24, 27, 201, 489
FokI GGATG 1 cut(s) 16
Fsp4HI GCNGC 4 cut(s) 24, 27, 201, 489
FspBI CTAG 3 cut(s) 84, 521, 617
GlaI GCGC 2 cut(s) 65, 248
GluI GCNGC 4 cut(s) 24, 27, 201, 489
HaeII RGCGCY 1 cut(s) 250
HapII CCGG 1 cut(s) 576
HhaI GCGC 2 cut(s) 66, 249
Hin1II CATG 3 cut(s) 383, 509, 670
Hin6I GCGC 2 cut(s) 64, 247
HinP1I GCGC 2 cut(s) 64, 247
HindIII AAGCTT 3 cut(s) 445, 475, 687
HpaII CCGG 1 cut(s) 576
HphI GGTGA 2 cut(s) 347, 443
Hpy166II GTNNAC 2 cut(s) 302, 749
Hpy188I TCNGA 1 cut(s) 374
Hpy188III TCNNGA 3 cut(s) 314, 554, 556
Hpy8I GTNNAC 2 cut(s) 302, 749
HpyAV CCTTC 1 cut(s) 175
HpyCH4III ACNGT 1 cut(s) 640
HpyCH4IV ACGT 1 cut(s) 298
HpyCH4V TGCA 6 cut(s) 29, 145, 239, 505, 563, 581
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpyF3I CTNAG 5 cut(s) 371, 448, 473, 764, 822
HpySE526I ACGT 1 cut(s) 298
Hsp92II CATG 3 cut(s) 383, 509, 670
HspAI GCGC 2 cut(s) 64, 247
Kzo9I GATC 3 cut(s) 36, 97, 337
LguI GCTCTTC 1 cut(s) 360
LmnI GCTCC 1 cut(s) 160
Lsp1109I GCAGC 4 cut(s) 13, 35, 187, 500
LweI GCATC 3 cut(s) 38, 187, 226
MaeI CTAG 3 cut(s) 84, 521, 617
MaeII ACGT 1 cut(s) 298
MaeIII GTNAC 6 cut(s) 127, 431, 451, 662, 705, 767
MalI GATC 3 cut(s) 38, 99, 339
MboI GATC 3 cut(s) 36, 97, 337
MboII GAAGA 4 cut(s) 131, 347, 536, 797
MhlI GDGCHC 1 cut(s) 658
MluCI AATT 5 cut(s) 54, 73, 231, 413, 515
MnlI CCTC 7 cut(s) 151, 171, 478, 520, 636, 639, 800
MroXI GAANNNNTTC 1 cut(s) 57
MseI TTAA 5 cut(s) 78, 321, 426, 572, 782
MspA1I CMGCKG 2 cut(s) 26, 223
MspI CCGG 1 cut(s) 576
MspR9I CCNGG 1 cut(s) 293
MvaI CCWGG 1 cut(s) 293
MwoI GCNNNNNNNGC 1 cut(s) 113
NdeI CATATG 1 cut(s) 141
NdeII GATC 3 cut(s) 36, 97, 337
NlaIII CATG 3 cut(s) 383, 509, 670
NmeAIII GCCGAG 1 cut(s) 637
NmuCI GTSAC 2 cut(s) 431, 767
NspI RCATGY 1 cut(s) 670
PaeR7I CTCGAG 1 cut(s) 554
PciI ACATGT 1 cut(s) 666
PciSI GCTCTTC 1 cut(s) 360
PdmI GAANNNNTTC 1 cut(s) 57
PflMI CCANNNNNTGG 1 cut(s) 499
PinAI ACCGGT 1 cut(s) 575
PkrI GCNGC 4 cut(s) 25, 28, 202, 490
PscI ACATGT 1 cut(s) 666
PshBI ATTAAT 1 cut(s) 321
PsiI TTATAA 1 cut(s) 776
Psp6I CCWGG 1 cut(s) 291
PspGI CCWGG 1 cut(s) 291
PstNI CAGNNNCTG 1 cut(s) 26
PvuII CAGCTG 2 cut(s) 26, 223
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
SapI GCTCTTC 1 cut(s) 360
SaqAI TTAA 5 cut(s) 78, 321, 426, 572, 782
SatI GCNGC 4 cut(s) 24, 27, 201, 489
Sau3AI GATC 3 cut(s) 36, 97, 337
ScrFI CCNGG 1 cut(s) 293
SduI GDGCHC 1 cut(s) 658
SfaNI GCATC 3 cut(s) 38, 187, 226
Sfr274I CTCGAG 1 cut(s) 554
SlaI CTCGAG 1 cut(s) 554
SmlI CTYRAG 2 cut(s) 132, 554
SmoI CTYRAG 2 cut(s) 132, 554
Sse9I AATT 5 cut(s) 54, 73, 231, 413, 515
SspMI CTAG 3 cut(s) 84, 521, 617
StyD4I CCNGG 1 cut(s) 291
StyI CCWWGG 1 cut(s) 250
TaaI ACNGT 1 cut(s) 640
TaiI ACGT 1 cut(s) 301
TaqI TCGA 2 cut(s) 364, 555
TasI AATT 5 cut(s) 54, 73, 231, 413, 515
TatI WGTACW 1 cut(s) 342
Tru1I TTAA 5 cut(s) 78, 321, 426, 572, 782
Tru9I TTAA 5 cut(s) 78, 321, 426, 572, 782
TscAI CASTG 3 cut(s) 199, 749, 772
TseFI GTSAC 2 cut(s) 431, 767
TseI GCWGC 4 cut(s) 23, 26, 200, 488
Tsp45I GTSAC 2 cut(s) 431, 767
TspDTI ATGAA 2 cut(s) 699, 803
TspRI CASTG 3 cut(s) 199, 749, 772
Van91I CCANNNNNTGG 1 cut(s) 499
VspI ATTAAT 1 cut(s) 321
XapI RAATTY 2 cut(s) 73, 413
XceI RCATGY 1 cut(s) 670
XhoI CTCGAG 1 cut(s) 554
XmiI GTMKAC 1 cut(s) 301
XmnI GAANNNNTTC 1 cut(s) 57
XspI CTAG 3 cut(s) 84, 521, 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.