Rroxscaffold_2G00150550

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0020745
Physical Location & Seq
Forward (+)
87927201 .. 87930435
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00150550.1

Sequence Viewer

Length: 1008 bp
ATGGGCAGGTGGAAGCTTGAGGAGGACATAATACACGTTGGCACTGGCATCTCTCACCTCCAGGACACAAAGGAAAGATTTAACATCAAATTCCTGAGGCGAGACTCATCAAAATCTGCCCAACAGCTTGCGTCTCAGTATGATACTTTGCATGATACTGATCTGTCATCTTTCATCAACAAGATTCATATCTGGCTCAATCAATTGAGGTATGTTGCAGCAGGCAATAGACCCTTTCTTAAAACATACAATGGCACCTATAAAAATACTACAGTACCAGCCCAGAAGAACATCCAGGAAGCTCTCAAGCATGCCGGGCTTGGGTCACAGATCAAAGCCACTGTGCCCTTCAATGCTGTCATCTACTTCTCCCCTGACTCAGTCCCAGTTCCATCCACGGGAGACTTCCAGCCGGAAATTAAAGATAATGCTCCATTCACTGTCAACATATACCCTTTCCTCTGCCTGTATGGAAGTGCCTATCTCCCTTTTGAATTTGCTTTCTTTGATGCAGACATTGACACCGTTGATTGGTCTTTAACCAAAGCTGGGTTCCCTGAAATGAATATCATAGTTGGAGAGGTGGGTTGGTCAACTGATGGGGTTGTAAATGCCAATATCCCAAATGCCAAAAGGTTTGATCAGGGAATGCTTGAACATGCCTTGAGCGGAAATAGAACTCCAGCTAGGGAAGGCATGATTGATGTTTACCTTTACAGCCACATTGATGAAAATGCTAAAAGCATTGCTCCCGGCTGCTTTGAGAGGGATTGGGGCCTGTTCGAGTTTGATGGGAAGCTGAAATACTTGTTGGATTTGTGGGGTTCAGTGCAGAATAAGGGAGTTGTAGCCGTGGAAGGGATGTTCAAAAGGTGGTGTGTGCTGAATCCAGATGCTAAAGATTTGTATGGTTTGCCAAGTAGCGGTGACTATGCTTGTGGTCTTTCAGATTGTCCTGCCTTGCATTATGGTTCTTCTTGCAATAACCATAGTGTTTCTGTGGCGTGA

Protein Analysis

335

Amino Acids

37.33

Weight (kDa)

5.59

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 67 - 272 6.4e-23 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0020745)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004851.1_g000005
rosa_roxburghii Rroxscaffold_2G00150550 Rroxscaffold_2G00150580
rosa_rugosa Rorug02G0012700
rosa_samantha Rh2AG058800 Rh2BG057300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 254
AccBSI CCGCTC 1 cut(s) 669
AciI CCGC 2 cut(s) 669, 924
AcsI RAATTY 2 cut(s) 89, 494
AfaI GTAC 1 cut(s) 276
AfiI CCNNNNNNNGG 6 cut(s) 321, 398, 531, 549, 858, 923
AflIII ACRYGT 1 cut(s) 34
AgsI TTSAA 4 cut(s) 352, 494, 656, 868
AjnI CCWGG 2 cut(s) 60, 294
AjuI GAANNNNNNNTTGG 4 cut(s) 536, 568, 794, 826
AloI GAACNNNNNNTCC 2 cut(s) 848, 880
AluBI AGCT 6 cut(s) 16, 127, 302, 548, 686, 799
AluI AGCT 6 cut(s) 16, 127, 302, 548, 686, 799
Alw26I GTCTC 3 cut(s) 96, 138, 396
AoxI GGCC 1 cut(s) 775
ApeKI GCWGC 2 cut(s) 218, 756
ApoI RAATTY 2 cut(s) 89, 494
AspS9I GGNCC 1 cut(s) 775
AsuC2I CCSGG 2 cut(s) 316, 753
AsuHPI GGTGA 2 cut(s) 47, 938
AxyI CCTNAGG 1 cut(s) 95
BaeGI GKGCMC 1 cut(s) 348
BaeI ACNNNNGTAYC 2 cut(s) 258, 291
BanI GGYRCC 1 cut(s) 254
BarI GAAGNNNNNNTAC 2 cut(s) 788, 820
BbvI GCAGC 2 cut(s) 230, 743
BccI CCATC 3 cut(s) 400, 593, 785
BceAI ACGGC 1 cut(s) 836
BciT130I CCWGG 2 cut(s) 62, 296
BclI TGATCA 1 cut(s) 640
BcnI CCSGG 2 cut(s) 316, 753
BcoDI GTCTC 3 cut(s) 96, 138, 396
BfaI CTAG 1 cut(s) 687
BfmI CTRYAG 1 cut(s) 270
BisI GCNGC 2 cut(s) 219, 757
BlsI GCNGC 2 cut(s) 220, 758
Bme1390I CCNGG 4 cut(s) 62, 296, 316, 753
BmgT120I GGNCC 1 cut(s) 775
BmiI GGNNCC 3 cut(s) 256, 554, 776
BmrFI CCNGG 4 cut(s) 62, 296, 316, 753
BmrI ACTGGG 1 cut(s) 380
BmsI GCATC 3 cut(s) 57, 499, 883
BmuI ACTGGG 1 cut(s) 380
BpmI CTGGAG 2 cut(s) 44, 666
BpuEI CTTGAG 3 cut(s) 38, 290, 685
BpuMI CCSGG 2 cut(s) 316, 753
BsaBI GATNNNNATC 2 cut(s) 159, 188
BsaJI CCNNGG 2 cut(s) 396, 852
Bsc4I CCNNNNNNNGG 6 cut(s) 321, 398, 531, 549, 858, 923
Bse1I ACTGG 2 cut(s) 49, 386
Bse21I CCTNAGG 1 cut(s) 95
Bse3DI GCAATG 1 cut(s) 744
Bse8I GATNNNNATC 2 cut(s) 159, 188
BseBI CCWGG 2 cut(s) 62, 296
BseDI CCNNGG 2 cut(s) 396, 852
BseGI GGATG 3 cut(s) 291, 392, 867
BseJI GATNNNNATC 2 cut(s) 159, 188
BseLI CCNNNNNNNGG 6 cut(s) 321, 398, 531, 549, 858, 923
BseMI GCAATG 1 cut(s) 744
BseMII CTCAG 3 cut(s) 86, 149, 393
BseNI ACTGG 2 cut(s) 49, 386
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 348
BseXI GCAGC 2 cut(s) 230, 743
BseYI CCCAGC 1 cut(s) 548
BsgI GTGCAG 1 cut(s) 851
BshFI GGCC 1 cut(s) 777
BshNI GGYRCC 1 cut(s) 254
BsiSI CCGG 3 cut(s) 315, 413, 753
BslFI GGGAC 1 cut(s) 368
BslI CCNNNNNNNGG 6 cut(s) 321, 398, 531, 549, 858, 923
BsmAI GTCTC 3 cut(s) 96, 138, 396
BsmBI CGTCTC 1 cut(s) 138
BsmFI GGGAC 1 cut(s) 368
BsmI GAATGC 1 cut(s) 654
BsnI GGCC 1 cut(s) 777
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 3 cut(s) 160, 330, 640
BspACI CCGC 2 cut(s) 669, 924
BspANI GGCC 1 cut(s) 777
BspCNI CTCAG 3 cut(s) 87, 148, 392
BspLI GGNNCC 3 cut(s) 256, 554, 776
BspT107I GGYRCC 1 cut(s) 254
BsrBI CCGCTC 1 cut(s) 669
BsrDI GCAATG 1 cut(s) 744
BsrI ACTGG 2 cut(s) 49, 386
BssECI CCNNGG 2 cut(s) 396, 852
BssMI GATC 3 cut(s) 160, 330, 640
Bst2UI CCWGG 2 cut(s) 62, 296
Bst4CI ACNGT 4 cut(s) 274, 343, 442, 526
BstC8I GCNNGC 3 cut(s) 129, 223, 312
BstDEI CTNAG 3 cut(s) 95, 135, 379
BstDSI CCRYGG 2 cut(s) 396, 852
BstF5I GGATG 3 cut(s) 291, 392, 867
BstKTI GATC 3 cut(s) 163, 333, 643
BstMAI GTCTC 3 cut(s) 96, 138, 396
BstMBI GATC 3 cut(s) 160, 330, 640
BstMWI GCNNNNNNNGC 1 cut(s) 316
BstNI CCWGG 2 cut(s) 62, 296
BstNSI RCATGY 2 cut(s) 314, 662
BstSCI CCNGG 4 cut(s) 60, 294, 314, 751
BstSFI CTRYAG 1 cut(s) 270
BstSLI GKGCMC 1 cut(s) 348
BstV1I GCAGC 2 cut(s) 230, 743
Bsu36I CCTNAGG 1 cut(s) 95
BsuRI GGCC 1 cut(s) 777
BtgI CCRYGG 2 cut(s) 396, 852
BtsCI GGATG 3 cut(s) 291, 392, 867
BtsIMutI CAGTG 4 cut(s) 42, 339, 438, 834
Cac8I GCNNGC 3 cut(s) 129, 223, 312
Cfr13I GGNCC 1 cut(s) 775
CseI GACGC 1 cut(s) 120
Csp6I GTAC 1 cut(s) 275
CviAII CATG 4 cut(s) 152, 311, 659, 697
CviQI GTAC 1 cut(s) 275
DdeI CTNAG 3 cut(s) 95, 135, 379
DpnI GATC 3 cut(s) 162, 332, 642
DpnII GATC 3 cut(s) 160, 330, 640
Eco81I CCTNAGG 1 cut(s) 95
EcoO109I RGGNCCY 1 cut(s) 775
EcoRII CCWGG 2 cut(s) 60, 294
Esp3I CGTCTC 1 cut(s) 138
FaeI CATG 4 cut(s) 155, 314, 662, 700
FaqI GGGAC 1 cut(s) 368
FatI CATG 4 cut(s) 151, 310, 658, 696
FbaI TGATCA 1 cut(s) 640
Fnu4HI GCNGC 2 cut(s) 219, 757
FokI GGATG 3 cut(s) 278, 379, 874
Fsp4HI GCNGC 2 cut(s) 219, 757
FspBI CTAG 1 cut(s) 687
GluI GCNGC 2 cut(s) 219, 757
GsaI CCCAGC 1 cut(s) 552
GsuI CTGGAG 2 cut(s) 44, 666
HaeIII GGCC 1 cut(s) 777
HapII CCGG 3 cut(s) 315, 413, 753
HgaI GACGC 1 cut(s) 120
Hin1II CATG 4 cut(s) 155, 314, 662, 700
HincII GTYRAC 2 cut(s) 445, 594
HindII GTYRAC 2 cut(s) 445, 594
HindIII AAGCTT 1 cut(s) 14
HinfI GANTC 4 cut(s) 104, 184, 377, 886
HpaII CCGG 3 cut(s) 315, 413, 753
HphI GGTGA 2 cut(s) 47, 938
Hpy166II GTNNAC 3 cut(s) 445, 594, 709
Hpy188I TCNGA 1 cut(s) 949
Hpy188III TCNNGA 2 cut(s) 94, 890
Hpy8I GTNNAC 3 cut(s) 445, 594, 709
HpyAV CCTTC 3 cut(s) 358, 686, 851
HpyCH4III ACNGT 4 cut(s) 274, 343, 442, 526
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 6 cut(s) 151, 218, 512, 832, 964, 981
HpyF10VI GCNNNNNNNGC 1 cut(s) 316
HpyF3I CTNAG 3 cut(s) 95, 135, 379
HpySE526I ACGT 1 cut(s) 36
Hsp92II CATG 4 cut(s) 155, 314, 662, 700
Ksp22I TGATCA 1 cut(s) 640
Kzo9I GATC 3 cut(s) 160, 330, 640
LmnI GCTCC 2 cut(s) 436, 754
Lsp1109I GCAGC 2 cut(s) 230, 743
LweI GCATC 3 cut(s) 57, 499, 883
MaeI CTAG 1 cut(s) 687
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 2 cut(s) 324, 926
MalI GATC 3 cut(s) 162, 332, 642
MbiI CCGCTC 1 cut(s) 669
MboI GATC 3 cut(s) 160, 330, 640
MboII GAAGA 2 cut(s) 298, 966
MfeI CAATTG 1 cut(s) 203
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 4 cut(s) 89, 203, 417, 494
MlyI GAGTC 2 cut(s) 98, 371
MmeI TCCRAC 2 cut(s) 556, 792
MnlI CCTC 8 cut(s) 13, 16, 68, 90, 201, 470, 574, 759
MseI TTAA 4 cut(s) 81, 240, 420, 539
MslI CAYNNNNRTG 1 cut(s) 726
MspI CCGG 3 cut(s) 315, 413, 753
MspR9I CCNGG 4 cut(s) 62, 296, 316, 753
MunI CAATTG 1 cut(s) 203
Mva1269I GAATGC 1 cut(s) 654
MvaI CCWGG 2 cut(s) 62, 296
MwoI GCNNNNNNNGC 1 cut(s) 316
NciI CCSGG 2 cut(s) 316, 753
NdeII GATC 3 cut(s) 160, 330, 640
NlaIII CATG 4 cut(s) 155, 314, 662, 700
NlaIV GGNNCC 3 cut(s) 256, 554, 776
NmuCI GTSAC 2 cut(s) 324, 926
NspI RCATGY 2 cut(s) 314, 662
PaeI GCATGC 1 cut(s) 314
PctI GAATGC 1 cut(s) 654
PfeI GAWTC 2 cut(s) 184, 886
PflFI GACNNNGTC 1 cut(s) 380
PfoI TCCNGGA 2 cut(s) 60, 294
PkrI GCNGC 2 cut(s) 220, 758
PleI GAGTC 2 cut(s) 98, 371
PpsI GAGTC 2 cut(s) 98, 371
Psp6I CCWGG 2 cut(s) 60, 294
PspFI CCCAGC 1 cut(s) 548
PspGI CCWGG 2 cut(s) 60, 294
PspN4I GGNNCC 3 cut(s) 256, 554, 776
PspPI GGNCC 1 cut(s) 775
PsyI GACNNNGTC 1 cut(s) 380
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
RseI CAYNNNNRTG 1 cut(s) 726
SaqAI TTAA 4 cut(s) 81, 240, 420, 539
SatI GCNGC 2 cut(s) 219, 757
Sau3AI GATC 3 cut(s) 160, 330, 640
Sau96I GGNCC 1 cut(s) 775
SchI GAGTC 2 cut(s) 98, 371
ScrFI CCNGG 4 cut(s) 62, 296, 316, 753
SduI GDGCHC 1 cut(s) 348
SfaNI GCATC 3 cut(s) 57, 499, 883
SfcI CTRYAG 1 cut(s) 270
SmiMI CAYNNNNRTG 1 cut(s) 726
SmlI CTYRAG 3 cut(s) 17, 305, 664
SmoI CTYRAG 3 cut(s) 17, 305, 664
SphI GCATGC 1 cut(s) 314
Sse9I AATT 4 cut(s) 89, 203, 417, 494
SsiI CCGC 2 cut(s) 669, 924
SspMI CTAG 1 cut(s) 687
StyD4I CCNGG 4 cut(s) 60, 294, 314, 751
TaaI ACNGT 4 cut(s) 274, 343, 442, 526
TaiI ACGT 1 cut(s) 39
TaqI TCGA 1 cut(s) 783
TasI AATT 4 cut(s) 89, 203, 417, 494
TfiI GAWTC 2 cut(s) 184, 886
Tru1I TTAA 4 cut(s) 81, 240, 420, 539
Tru9I TTAA 4 cut(s) 81, 240, 420, 539
TscAI CASTG 4 cut(s) 49, 346, 445, 834
TseFI GTSAC 2 cut(s) 324, 926
TseI GCWGC 2 cut(s) 218, 756
Tsp45I GTSAC 2 cut(s) 324, 926
TspDTI ATGAA 4 cut(s) 163, 176, 578, 744
TspRI CASTG 4 cut(s) 49, 346, 445, 834
Tth111I GACNNNGTC 1 cut(s) 380
XapI RAATTY 2 cut(s) 89, 494
XceI RCATGY 2 cut(s) 314, 662
XspI CTAG 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.