Rroxscaffold_2G00150580

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0020745
Physical Location & Seq
Forward (+)
87950458 .. 87951778
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00150580.1

Sequence Viewer

Length: 834 bp
ATGCAAGAAGGAAGCACAAGGAGAATGCAATTAAGGTATGTTGCAGCAGGCAGTAGACCCTTTCTTAAAACATACAATGGCACCTATAAAAATACTACAGTACCAGCCCAGAAGAACATCCAGGAAGCTCTCAACCGTGCCGGGCTTGCTGTCATCTACTTCTCCCCTGACTCAAACCCAGTTCCATCCACGGGAGACTTCCAGCCGGAAATTAAAGATAATGCGATTGAGATAATCCAATACCTATACACCAATGATGCTCCATTCACTGTCAACATCTACCCTTTCCTCTGCCTGTATGGAAGTGCCTATCTCCCTTTTGAATTTGCTTTCTTTGATGCAGACATTGACACCGTTGATTGGTCTTTAACCAAAGCTGGGTTCCCTGAAATGAATATCATAGTTGGAGAGGGGGTTGGTCAACTGATTGTTTTAAATGCCAATATCCCAAATGCCAAAAGGTTTGATCAGGGAATGCCTGAACATGCCTTGAGCGGAAATGGAACTCCAGCTACGGAAGGCATGATTGATGTTTACCTTTACAGCCACATTGATGAAAATGCAAAAAGCATTGCTCCCGGCTGCTTTGAGAGGGATTGGGGCCTGTTCGAGTTCGATGGGAAGCTGAAATACGACTTGGATTTGTCGGGTTCAGTGCAGAATAAGGGAATTGTAGCCGTGGAAGGGATGTTCAAAAGGTGGTGTGTGCTGAATCCAGATGCTAAAGATTTGTTTGGTTTTCCAAGTAGCGTTGACTATGCTTGTGGTCTTTCAGATTGTACTGCCTTGGGTTATGGTTCTTCTTGCAATAACCATAGCGTTTCTGTGGCGTGA

Protein Analysis

277

Amino Acids

30.44

Weight (kDa)

4.63

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 10 - 214 1.6e-17 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020745)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004851.1_g000005
rosa_roxburghii Rroxscaffold_2G00150550 Rroxscaffold_2G00150580
rosa_rugosa Rorug02G0012700
rosa_samantha Rh2AG058800 Rh2BG057300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 80
AccBSI CCGCTC 1 cut(s) 495
AccI GTMKAC 1 cut(s) 55
AciI CCGC 1 cut(s) 495
AcsI RAATTY 1 cut(s) 323
AfaI GTAC 2 cut(s) 102, 781
AfiI CCNNNNNNNGG 4 cut(s) 191, 360, 378, 684
AgsI TTSAA 2 cut(s) 323, 694
AjnI CCWGG 1 cut(s) 120
AjuI GAANNNNNNNTTGG 4 cut(s) 365, 397, 620, 652
AloI GAACNNNNNNTCC 2 cut(s) 674, 706
AluBI AGCT 4 cut(s) 128, 377, 512, 625
AluI AGCT 4 cut(s) 128, 377, 512, 625
Alw26I GTCTC 1 cut(s) 189
AoxI GGCC 1 cut(s) 601
ApeKI GCWGC 2 cut(s) 44, 582
ApoI RAATTY 1 cut(s) 323
AspS9I GGNCC 1 cut(s) 601
AsuC2I CCSGG 2 cut(s) 142, 579
BaeI ACNNNNGTAYC 2 cut(s) 84, 117
BanI GGYRCC 1 cut(s) 80
BarI GAAGNNNNNNTAC 2 cut(s) 614, 646
BbvI GCAGC 2 cut(s) 56, 569
BccI CCATC 2 cut(s) 193, 611
BceAI ACGGC 1 cut(s) 662
BciT130I CCWGG 1 cut(s) 122
BclI TGATCA 1 cut(s) 466
BcnI CCSGG 2 cut(s) 142, 579
BcoDI GTCTC 1 cut(s) 189
BfmI CTRYAG 1 cut(s) 96
BisI GCNGC 2 cut(s) 45, 583
BlsI GCNGC 2 cut(s) 46, 584
Bme1390I CCNGG 3 cut(s) 122, 142, 579
BmgT120I GGNCC 1 cut(s) 601
BmiI GGNNCC 3 cut(s) 82, 383, 602
BmrFI CCNGG 3 cut(s) 122, 142, 579
BmrI ACTGGG 1 cut(s) 173
BmsI GCATC 3 cut(s) 247, 328, 709
BmuI ACTGGG 1 cut(s) 173
BpmI CTGGAG 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 511
BpuMI CCSGG 2 cut(s) 142, 579
BsaJI CCNNGG 3 cut(s) 189, 678, 786
BsaXI ACNNNNNCTCC 2 cut(s) 399, 429
Bsc4I CCNNNNNNNGG 4 cut(s) 191, 360, 378, 684
Bse1I ACTGG 1 cut(s) 179
Bse3DI GCAATG 1 cut(s) 570
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 3 cut(s) 189, 678, 786
BseGI GGATG 3 cut(s) 117, 185, 693
BseLI CCNNNNNNNGG 4 cut(s) 191, 360, 378, 684
BseMI GCAATG 1 cut(s) 570
BseNI ACTGG 1 cut(s) 179
BseXI GCAGC 2 cut(s) 56, 569
BseYI CCCAGC 1 cut(s) 377
BsgI GTGCAG 1 cut(s) 677
BshFI GGCC 1 cut(s) 603
BshNI GGYRCC 1 cut(s) 80
BsiSI CCGG 3 cut(s) 141, 206, 579
BslI CCNNNNNNNGG 4 cut(s) 191, 360, 378, 684
BsmAI GTCTC 1 cut(s) 189
BsmI GAATGC 2 cut(s) 30, 480
BsnI GGCC 1 cut(s) 603
Bsp143I GATC 1 cut(s) 466
BspACI CCGC 1 cut(s) 495
BspANI GGCC 1 cut(s) 603
BspLI GGNNCC 3 cut(s) 82, 383, 602
BspT107I GGYRCC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 495
BsrDI GCAATG 1 cut(s) 570
BsrI ACTGG 1 cut(s) 179
BssECI CCNNGG 3 cut(s) 189, 678, 786
BssMI GATC 1 cut(s) 466
BssT1I CCWWGG 1 cut(s) 786
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 4 cut(s) 100, 137, 271, 355
BstC8I GCNNGC 2 cut(s) 49, 147
BstDSI CCRYGG 2 cut(s) 189, 678
BstF5I GGATG 3 cut(s) 117, 185, 693
BstKTI GATC 1 cut(s) 469
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 1 cut(s) 466
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 1 cut(s) 122
BstNSI RCATGY 1 cut(s) 488
BstSCI CCNGG 3 cut(s) 120, 140, 577
BstSFI CTRYAG 1 cut(s) 96
BstV1I GCAGC 2 cut(s) 56, 569
BsuRI GGCC 1 cut(s) 603
BtgI CCRYGG 2 cut(s) 189, 678
BtsCI GGATG 3 cut(s) 117, 185, 693
BtsIMutI CAGTG 2 cut(s) 267, 660
Cac8I GCNNGC 2 cut(s) 49, 147
Cfr13I GGNCC 1 cut(s) 601
Csp6I GTAC 2 cut(s) 101, 780
CviAII CATG 2 cut(s) 485, 523
CviQI GTAC 2 cut(s) 101, 780
DpnI GATC 1 cut(s) 468
DpnII GATC 1 cut(s) 466
DraI TTTAAA 1 cut(s) 435
Eco130I CCWWGG 1 cut(s) 786
EcoO109I RGGNCCY 1 cut(s) 601
EcoRII CCWGG 1 cut(s) 120
EcoT14I CCWWGG 1 cut(s) 786
ErhI CCWWGG 1 cut(s) 786
FaeI CATG 2 cut(s) 488, 526
FatI CATG 2 cut(s) 484, 522
FbaI TGATCA 1 cut(s) 466
FblI GTMKAC 1 cut(s) 55
Fnu4HI GCNGC 2 cut(s) 45, 583
FokI GGATG 3 cut(s) 104, 172, 700
Fsp4HI GCNGC 2 cut(s) 45, 583
GluI GCNGC 2 cut(s) 45, 583
GsaI CCCAGC 1 cut(s) 381
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 1 cut(s) 603
HapII CCGG 3 cut(s) 141, 206, 579
Hin1II CATG 2 cut(s) 488, 526
HincII GTYRAC 3 cut(s) 274, 422, 754
HindII GTYRAC 3 cut(s) 274, 422, 754
HinfI GANTC 2 cut(s) 170, 712
HpaII CCGG 3 cut(s) 141, 206, 579
Hpy166II GTNNAC 5 cut(s) 56, 274, 422, 535, 754
Hpy188I TCNGA 1 cut(s) 775
Hpy188III TCNNGA 1 cut(s) 716
Hpy8I GTNNAC 5 cut(s) 56, 274, 422, 535, 754
HpyAV CCTTC 2 cut(s) 512, 677
HpyCH4III ACNGT 4 cut(s) 100, 137, 271, 355
HpyCH4V TGCA 7 cut(s) 4, 28, 44, 341, 563, 658, 807
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
Hsp92II CATG 2 cut(s) 488, 526
Ksp22I TGATCA 1 cut(s) 466
Kzo9I GATC 1 cut(s) 466
LmnI GCTCC 2 cut(s) 265, 580
Lsp1109I GCAGC 2 cut(s) 56, 569
LweI GCATC 3 cut(s) 247, 328, 709
MalI GATC 1 cut(s) 468
MbiI CCGCTC 1 cut(s) 495
MboI GATC 1 cut(s) 466
MboII GAAGA 2 cut(s) 124, 792
MluCI AATT 4 cut(s) 29, 210, 323, 669
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 1 cut(s) 385
MnlI CCTC 3 cut(s) 299, 403, 585
MseI TTAA 5 cut(s) 32, 66, 213, 368, 434
MslI CAYNNNNRTG 1 cut(s) 552
MspI CCGG 3 cut(s) 141, 206, 579
MspR9I CCNGG 3 cut(s) 122, 142, 579
Mva1269I GAATGC 2 cut(s) 30, 480
MvaI CCWGG 1 cut(s) 122
MwoI GCNNNNNNNGC 1 cut(s) 146
NciI CCSGG 2 cut(s) 142, 579
NdeII GATC 1 cut(s) 466
NlaIII CATG 2 cut(s) 488, 526
NlaIV GGNNCC 3 cut(s) 82, 383, 602
NspI RCATGY 1 cut(s) 488
PctI GAATGC 2 cut(s) 30, 480
PfeI GAWTC 1 cut(s) 712
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 2 cut(s) 46, 584
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
Psp6I CCWGG 1 cut(s) 120
PspFI CCCAGC 1 cut(s) 377
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 3 cut(s) 82, 383, 602
PspPI GGNCC 1 cut(s) 601
PsrI GAACNNNNNNTAC 2 cut(s) 496, 528
RsaI GTAC 2 cut(s) 102, 781
RsaNI GTAC 2 cut(s) 101, 780
RseI CAYNNNNRTG 1 cut(s) 552
SaqAI TTAA 5 cut(s) 32, 66, 213, 368, 434
SatI GCNGC 2 cut(s) 45, 583
Sau3AI GATC 1 cut(s) 466
Sau96I GGNCC 1 cut(s) 601
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 3 cut(s) 122, 142, 579
SfaNI GCATC 3 cut(s) 247, 328, 709
SfcI CTRYAG 1 cut(s) 96
SmiMI CAYNNNNRTG 1 cut(s) 552
SmlI CTYRAG 1 cut(s) 490
SmoI CTYRAG 1 cut(s) 490
Sse9I AATT 4 cut(s) 29, 210, 323, 669
SsiI CCGC 1 cut(s) 495
StyD4I CCNGG 3 cut(s) 120, 140, 577
StyI CCWWGG 1 cut(s) 786
TaaI ACNGT 4 cut(s) 100, 137, 271, 355
TaqI TCGA 2 cut(s) 609, 615
TasI AATT 4 cut(s) 29, 210, 323, 669
TatI WGTACW 1 cut(s) 779
TfiI GAWTC 1 cut(s) 712
Tru1I TTAA 5 cut(s) 32, 66, 213, 368, 434
Tru9I TTAA 5 cut(s) 32, 66, 213, 368, 434
TscAI CASTG 2 cut(s) 274, 660
TseI GCWGC 2 cut(s) 44, 582
TspDTI ATGAA 2 cut(s) 407, 570
TspGWI ACGGA 1 cut(s) 530
TspRI CASTG 2 cut(s) 274, 660
XapI RAATTY 1 cut(s) 323
XceI RCATGY 1 cut(s) 488
XmiI GTMKAC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.