Rh2BG057300

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0020745
Physical Location & Seq
Reverse (-)
4280613 .. 4280972
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG057300.1

Sequence Viewer

Length: 360 bp
ATGCTTGAACATGCCTTGAGCGGAAATGGAACTCTAGCTAGGGAAGGCATGATTGATGTTTACCTTTACAGCCACATTGATGAAAATGCTAAAAGCATTGCTCCCGGCTGCTTTGAGAGGGATTGGGGCCTGTTCGAGTTCGATGGGAAGCTGAAATACGAGTTGGATTTGGCGGCTTCAGTGCAGAATAAGGGAATTGTAGCCGTTGAAGGGATGTTCAAAAGGTGGTGTGTGCTGAATCCAGATGCTAAAGATTTGTATGGTTTGCCAAGTAGCGTTGACTATGCTTGTGGTCTTTCAGATTGTCCGGCCTTGGGTTATGGTTCTTCTTGCAATAACCATAGTGTTTCTGTGGCGTGA

Protein Analysis

119

Amino Acids

12.9

Weight (kDa)

4.71

Isoelectric Point (pI)

28.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 9 - 56 1e-06 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0020745)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004851.1_g000005
rosa_roxburghii Rroxscaffold_2G00150550 Rroxscaffold_2G00150580
rosa_rugosa Rorug02G0012700
rosa_samantha Rh2AG058800 Rh2BG057300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 21
AciI CCGC 2 cut(s) 21, 173
AcuI CTGAAG 1 cut(s) 162
AfiI CCNNNNNNNGG 2 cut(s) 210, 314
AgsI TTSAA 3 cut(s) 8, 209, 220
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AluBI AGCT 2 cut(s) 38, 151
AluI AGCT 2 cut(s) 38, 151
AoxI GGCC 2 cut(s) 127, 309
ApeKI GCWGC 1 cut(s) 108
AspS9I GGNCC 1 cut(s) 127
AsuC2I CCSGG 1 cut(s) 105
BarI GAAGNNNNNNTAC 2 cut(s) 140, 172
BbvI GCAGC 1 cut(s) 95
BccI CCATC 1 cut(s) 137
BceAI ACGGC 1 cut(s) 188
BcnI CCSGG 1 cut(s) 105
BfaI CTAG 2 cut(s) 35, 39
BisI GCNGC 2 cut(s) 109, 174
BlsI GCNGC 2 cut(s) 110, 175
Bme1390I CCNGG 1 cut(s) 105
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 1 cut(s) 128
BmrFI CCNGG 1 cut(s) 105
BmsI GCATC 1 cut(s) 235
BpuEI CTTGAG 1 cut(s) 37
BpuMI CCSGG 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 312
Bsc4I CCNNNNNNNGG 2 cut(s) 210, 314
Bse3DI GCAATG 1 cut(s) 96
BseDI CCNNGG 1 cut(s) 312
BseGI GGATG 1 cut(s) 219
BseLI CCNNNNNNNGG 2 cut(s) 210, 314
BseMI GCAATG 1 cut(s) 96
BseXI GCAGC 1 cut(s) 95
BsgI GTGCAG 1 cut(s) 203
BshFI GGCC 2 cut(s) 129, 311
BsiSI CCGG 2 cut(s) 105, 308
BslI CCNNNNNNNGG 2 cut(s) 210, 314
BsnI GGCC 2 cut(s) 129, 311
BspACI CCGC 2 cut(s) 21, 173
BspANI GGCC 2 cut(s) 129, 311
BspLI GGNNCC 1 cut(s) 128
BsrBI CCGCTC 1 cut(s) 21
BsrDI GCAATG 1 cut(s) 96
BssECI CCNNGG 1 cut(s) 312
BssT1I CCWWGG 1 cut(s) 312
BstF5I GGATG 1 cut(s) 219
BstNSI RCATGY 1 cut(s) 14
BstSCI CCNGG 1 cut(s) 103
BstV1I GCAGC 1 cut(s) 95
BsuRI GGCC 2 cut(s) 129, 311
BtsCI GGATG 1 cut(s) 219
BtsIMutI CAGTG 1 cut(s) 186
Cfr13I GGNCC 1 cut(s) 127
CviAII CATG 2 cut(s) 11, 49
CviJI RGCY 8 cut(s) 38, 72, 108, 129, 151, 176, 203, 311
CviKI_1 RGCY 8 cut(s) 38, 72, 108, 129, 151, 176, 203, 311
Eco130I CCWWGG 1 cut(s) 312
Eco57I CTGAAG 1 cut(s) 162
EcoO109I RGGNCCY 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 312
FaeI CATG 2 cut(s) 14, 52
FaiI YATR 6 cut(s) 12, 50, 261, 285, 321, 342
FatI CATG 2 cut(s) 10, 48
Fnu4HI GCNGC 2 cut(s) 109, 174
FokI GGATG 1 cut(s) 226
Fsp4HI GCNGC 2 cut(s) 109, 174
FspBI CTAG 2 cut(s) 35, 39
GluI GCNGC 2 cut(s) 109, 174
HaeIII GGCC 2 cut(s) 129, 311
HapII CCGG 2 cut(s) 105, 308
Hin1II CATG 2 cut(s) 14, 52
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 1 cut(s) 238
HpaII CCGG 2 cut(s) 105, 308
Hpy166II GTNNAC 2 cut(s) 61, 280
Hpy188I TCNGA 1 cut(s) 301
Hpy188III TCNNGA 1 cut(s) 242
Hpy8I GTNNAC 2 cut(s) 61, 280
HpyAV CCTTC 2 cut(s) 38, 203
HpyCH4V TGCA 2 cut(s) 184, 333
Hsp92II CATG 2 cut(s) 14, 52
LmnI GCTCC 1 cut(s) 106
LpnPI CCDG 4 cut(s) 118, 143, 255, 321
Lsp1109I GCAGC 1 cut(s) 95
LweI GCATC 1 cut(s) 235
MaeI CTAG 2 cut(s) 35, 39
MbiI CCGCTC 1 cut(s) 21
MboII GAAGA 1 cut(s) 318
MluCI AATT 1 cut(s) 195
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 1 cut(s) 111
MslI CAYNNNNRTG 1 cut(s) 78
MspI CCGG 2 cut(s) 105, 308
MspR9I CCNGG 1 cut(s) 105
NciI CCSGG 1 cut(s) 105
NlaIII CATG 2 cut(s) 14, 52
NlaIV GGNNCC 1 cut(s) 128
NspI RCATGY 1 cut(s) 14
PfeI GAWTC 1 cut(s) 238
PkrI GCNGC 2 cut(s) 110, 175
PspN4I GGNNCC 1 cut(s) 128
PspPI GGNCC 1 cut(s) 127
RseI CAYNNNNRTG 1 cut(s) 78
SatI GCNGC 2 cut(s) 109, 174
Sau96I GGNCC 1 cut(s) 127
ScrFI CCNGG 1 cut(s) 105
SetI ASST 4 cut(s) 40, 66, 153, 227
SfaNI GCATC 1 cut(s) 235
SmiMI CAYNNNNRTG 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 16
SmoI CTYRAG 1 cut(s) 16
Sse9I AATT 1 cut(s) 195
SsiI CCGC 2 cut(s) 21, 173
SspMI CTAG 2 cut(s) 35, 39
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 1 cut(s) 312
TaqI TCGA 2 cut(s) 135, 141
TasI AATT 1 cut(s) 195
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 1 cut(s) 238
TscAI CASTG 1 cut(s) 186
TseI GCWGC 1 cut(s) 108
TspDTI ATGAA 1 cut(s) 96
TspRI CASTG 1 cut(s) 186
XceI RCATGY 1 cut(s) 14
XspI CTAG 2 cut(s) 35, 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.