Rroxscaffold_3G00245780

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0022264
Physical Location & Seq
Reverse (-)
38877955 .. 38882527
4573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00245780.1

Sequence Viewer

Length: 618 bp
ATGAAGATCTGCGCAAAATATCAACAGCTTTTCACAATTTTCTCGGACGAAGAGAGGATTCAGGTGGAGCGAGCTGTTTATAGTGATGCTGATATCTATCTCCTTGATGACCCCTTTAGTGCCGTGGATGCACATACAGCTGCAATTCTATTTCATATTTCAAATGACTTGCCGGATGAAGAAGTCTTTCATGCAGATGTTCTCCCTACTTGGAAAATGTTCTTTAATGAGTCTTCATCGGCAGATGGGGCAAAAACTGGTGTTGTCTTCGTTTCCCGACAAAGGGAGATATTACCTTATGCTTTTACTCTTGGTGAACTACACTCCAATAATTTTGCTATGTACCAAGCGCTAATCATGGGACTACAAACGGCAGCTGAAATGAAAATCTCAAATCTAGAGATATATGGAGACTCGAAGTTAGTAGTTGATCAATTACTAACTATTTATAAAGTGAAGAAAGAGGATTTAGTTCCTTATTTTCAGTTCGCCACACAACTCTTGAAGGGTTTTGATGTTATCACGTTGATGCATGTCTTTGAGAAAGGAAAATCTAATGGCAGATGCTTTGGCCAACTTAGTAGCAACTTTGACAATGTCGAGAGGATGAGATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

205

Amino Acids

23.37

Weight (kDa)

4.99

Isoelectric Point (pI)

30.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 98 - 180 1.7e-10 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022264)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g04124
malus_domestica MD17G1175600.v1.1
rosa_chinensis RchiOBHm_Chr2g0142741
rosa_roxburghii Rroxscaffold_3G00245780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
Acc16I TGCGCA 1 cut(s) 13
AcoI YGGCCR 1 cut(s) 571
AfaI GTAC 1 cut(s) 344
AfeI AGCGCT 1 cut(s) 351
AfiI CCNNNNNNNGG 2 cut(s) 282, 283
AgsI TTSAA 2 cut(s) 162, 505
AluBI AGCT 4 cut(s) 28, 74, 140, 377
AluI AGCT 4 cut(s) 28, 74, 140, 377
Alw26I GTCTC 1 cut(s) 405
Aor51HI AGCGCT 1 cut(s) 351
AoxI GGCC 1 cut(s) 571
ApeKI GCWGC 2 cut(s) 140, 374
Asp700I GAANNNNTTC 2 cut(s) 186, 218
AspLEI GCGC 2 cut(s) 14, 352
AsuHPI GGTGA 1 cut(s) 326
BalI TGGCCA 1 cut(s) 573
BbsI GAAGAC 2 cut(s) 225, 259
BbvI GCAGC 2 cut(s) 127, 386
BccI CCATC 1 cut(s) 239
BceAI ACGGC 2 cut(s) 107, 387
BclI TGATCA 1 cut(s) 430
BcoDI GTCTC 1 cut(s) 405
BfaI CTAG 1 cut(s) 398
BfoI RGCGCY 1 cut(s) 353
BglII AGATCT 1 cut(s) 6
BisI GCNGC 2 cut(s) 141, 375
BlsI GCNGC 2 cut(s) 142, 376
BmsI GCATC 4 cut(s) 76, 118, 519, 554
BpiI GAAGAC 2 cut(s) 225, 259
BsaBI GATNNNNATC 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 123
Bsc4I CCNNNNNNNGG 2 cut(s) 282, 283
Bse1I ACTGG 1 cut(s) 262
Bse8I GATNNNNATC 1 cut(s) 96
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 3 cut(s) 133, 181, 612
BseJI GATNNNNATC 1 cut(s) 96
BseLI CCNNNNNNNGG 2 cut(s) 282, 283
BseNI ACTGG 1 cut(s) 262
BseXI GCAGC 2 cut(s) 127, 386
BshFI GGCC 1 cut(s) 573
BsiSI CCGG 1 cut(s) 173
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 2 cut(s) 282, 283
BsmAI GTCTC 1 cut(s) 405
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 1 cut(s) 573
Bsp143I GATC 3 cut(s) 6, 430, 611
BspANI GGCC 1 cut(s) 573
BsrI ACTGG 1 cut(s) 262
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 3 cut(s) 6, 430, 611
Bst6I CTCTTC 1 cut(s) 45
BstC8I GCNNGC 1 cut(s) 72
BstDEI CTNAG 1 cut(s) 578
BstDSI CCRYGG 1 cut(s) 123
BstF5I GGATG 3 cut(s) 133, 181, 612
BstH2I RGCGCY 1 cut(s) 353
BstHHI GCGC 2 cut(s) 14, 352
BstKTI GATC 3 cut(s) 9, 433, 614
BstMAI GTCTC 1 cut(s) 405
BstMBI GATC 3 cut(s) 6, 430, 611
BstMWI GCNNNNNNNGC 3 cut(s) 128, 137, 248
BstNSI RCATGY 1 cut(s) 536
BstV1I GCAGC 2 cut(s) 127, 386
BstV2I GAAGAC 2 cut(s) 225, 259
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BsuRI GGCC 1 cut(s) 573
BtgI CCRYGG 1 cut(s) 123
BtsCI GGATG 3 cut(s) 133, 181, 612
Cac8I GCNNGC 1 cut(s) 72
CfoI GCGC 2 cut(s) 14, 352
Csp6I GTAC 1 cut(s) 343
CviAII CATG 3 cut(s) 191, 358, 533
CviJI RGCY 5 cut(s) 28, 74, 140, 377, 573
CviKI_1 RGCY 5 cut(s) 28, 74, 140, 377, 573
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 1 cut(s) 578
DpnI GATC 3 cut(s) 8, 432, 613
DpnII GATC 3 cut(s) 6, 430, 611
EaeI YGGCCR 1 cut(s) 571
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Eco32I GATATC 1 cut(s) 94
Eco47III AGCGCT 1 cut(s) 351
EcoRV GATATC 1 cut(s) 94
EcoT22I ATGCAT 1 cut(s) 534
FaeI CATG 3 cut(s) 194, 361, 536
FaqI GGGAC 1 cut(s) 375
FatI CATG 3 cut(s) 190, 357, 532
FbaI TGATCA 1 cut(s) 430
Fnu4HI GCNGC 2 cut(s) 141, 375
FokI GGATG 2 cut(s) 140, 188
Fsp4HI GCNGC 2 cut(s) 141, 375
FspBI CTAG 1 cut(s) 398
FspI TGCGCA 1 cut(s) 13
GlaI GCGC 2 cut(s) 13, 351
GluI GCNGC 2 cut(s) 141, 375
HaeII RGCGCY 1 cut(s) 353
HaeIII GGCC 1 cut(s) 573
HapII CCGG 1 cut(s) 173
HhaI GCGC 2 cut(s) 14, 352
Hin1II CATG 3 cut(s) 194, 361, 536
Hin6I GCGC 2 cut(s) 12, 350
HinP1I GCGC 2 cut(s) 12, 350
HinfI GANTC 3 cut(s) 58, 230, 413
HpaII CCGG 1 cut(s) 173
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 1 cut(s) 46
Hpy188III TCNNGA 4 cut(s) 276, 398, 502, 601
Hpy8I GTNNAC 1 cut(s) 317
HpyAV CCTTC 1 cut(s) 499
HpyCH4IV ACGT 1 cut(s) 524
HpyCH4V TGCA 4 cut(s) 131, 143, 194, 532
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 137, 248
HpyF3I CTNAG 1 cut(s) 578
HpySE526I ACGT 1 cut(s) 524
Hsp92II CATG 3 cut(s) 194, 361, 536
HspAI GCGC 2 cut(s) 12, 350
Ksp22I TGATCA 1 cut(s) 430
Kzo9I GATC 3 cut(s) 6, 430, 611
LmnI GCTCC 1 cut(s) 67
LpnPI CCDG 3 cut(s) 47, 186, 243
Lsp1109I GCAGC 2 cut(s) 127, 386
LweI GCATC 4 cut(s) 76, 118, 519, 554
MaeI CTAG 1 cut(s) 398
MaeII ACGT 1 cut(s) 524
MalI GATC 3 cut(s) 8, 432, 613
MboI GATC 3 cut(s) 6, 430, 611
MboII GAAGA 6 cut(s) 16, 62, 191, 225, 259, 469
MflI RGATCY 1 cut(s) 6
MlsI TGGCCA 1 cut(s) 573
MluCI AATT 4 cut(s) 36, 144, 331, 434
MluNI TGGCCA 1 cut(s) 573
MlyI GAGTC 2 cut(s) 239, 407
MnlI CCTC 3 cut(s) 48, 457, 597
Mox20I TGGCCA 1 cut(s) 573
Mph1103I ATGCAT 1 cut(s) 534
MroXI GAANNNNTTC 2 cut(s) 186, 218
MscI TGGCCA 1 cut(s) 573
MseI TTAA 1 cut(s) 225
MslI CAYNNNNRTG 2 cut(s) 195, 527
Msp20I TGGCCA 1 cut(s) 573
MspA1I CMGCKG 2 cut(s) 140, 377
MspI CCGG 1 cut(s) 173
MwoI GCNNNNNNNGC 3 cut(s) 128, 137, 248
NdeII GATC 3 cut(s) 6, 430, 611
NlaIII CATG 3 cut(s) 194, 361, 536
NsbI TGCGCA 1 cut(s) 13
NsiI ATGCAT 1 cut(s) 534
NspI RCATGY 1 cut(s) 536
PdmI GAANNNNTTC 2 cut(s) 186, 218
PfeI GAWTC 1 cut(s) 58
PflFI GACNNNGTC 1 cut(s) 596
PkrI GCNGC 2 cut(s) 142, 376
PleI GAGTC 2 cut(s) 238, 407
PpsI GAGTC 2 cut(s) 238, 407
PsiI TTATAA 1 cut(s) 450
PsuI RGATCY 1 cut(s) 6
PsyI GACNNNGTC 1 cut(s) 596
PvuII CAGCTG 2 cut(s) 140, 377
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
RseI CAYNNNNRTG 2 cut(s) 195, 527
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 2 cut(s) 141, 375
Sau3AI GATC 3 cut(s) 6, 430, 611
SchI GAGTC 2 cut(s) 239, 407
SetI ASST 7 cut(s) 30, 66, 76, 142, 298, 379, 527
SfaNI GCATC 4 cut(s) 76, 118, 519, 554
SmiMI CAYNNNNRTG 2 cut(s) 195, 527
Sse9I AATT 4 cut(s) 36, 144, 331, 434
SspMI CTAG 1 cut(s) 398
TaiI ACGT 1 cut(s) 527
TaqI TCGA 2 cut(s) 416, 600
TasI AATT 4 cut(s) 36, 144, 331, 434
TfiI GAWTC 1 cut(s) 58
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TseI GCWGC 2 cut(s) 140, 374
TspDTI ATGAA 6 cut(s) 17, 143, 179, 192, 225, 398
Tth111I GACNNNGTC 1 cut(s) 596
XbaI TCTAGA 1 cut(s) 397
XceI RCATGY 1 cut(s) 536
XmnI GAANNNNTTC 2 cut(s) 186, 218
XspI CTAG 1 cut(s) 398
Zsp2I ATGCAT 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.