Rroxscaffold_3G00268070

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0017663
Physical Location & Seq
Forward (+)
61236562 .. 61237338
777 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_3G00268070.1

Sequence Viewer

Length: 702 bp
ATGGTTGCGTCTCTCAAAGAACACGCCAGAGAGGTCGGAATGCCTGAATGGGGAAATGTGTATCGTGTTGCAGAACAATTGCAATTTGCCGAAAATGGTAATGATTCTTGGAATACGCCGTACGGTGCATTTCTGCTTGAATGGTACTCTGGAATGTTGCTTATGCACGGAGAAAGGATATGCGAGCAAGCTGAGGCCATTTTCTCGGGAACAAATGTTACTATGTCAGCAAAACTAGCAGGGAACTATTGCCATTACCTCACTCAGTCTCATGTTTCGGAGCTGACTGCTGGCTATTACAACACTTCAACAAGGGATGGATACTTGCCGTTTGCTCACATGTTTGCTAGGCAAACTATGTCTCTTAGGCATGGGTTTGCGTTATGCTGCTCCTGTTTTGACTTGAAAGATGAGATGGAAAGGTATCCCACCAGTAGTCCAGAAGGTCTTCTCAAACAGATGCTATCGGCTGCTAGGGTTTGTGGTATACCCGTGGAAGGTGAAACTTCTACATCCTTTTGCAATGGGAGACTCATATCGTTGAAACAAGTGGTAACAATGTCCAAGCATTACGTATCTGGACTAGAAAACCCCTCCTTCTCATTCAAGCTCAGGGCCAACAAGGAGGAGTTGTTCAATTCACCTAAATATTGGGCTATGTTTACTGATTTTGTCAGCCAAATGTCTAATGTCAATGATTAA

Protein Analysis

233

Amino Acids

26.3

Weight (kDa)

5.81

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 34 - 223 3.7e-30 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017663)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11340
rosa_chinensis RchiOBHm_Chr7g0186521
rosa_laevigata RLG00000004838
rosa_multiflora Rmu_sc0000645.1_g000026
rosa_roxburghii Rroxscaffold_3G00268070
rosa_rugosa Rorug06G0474900 Rorug06G0474900
rosa_samantha Rh7AG066300 Rh7BG082200 Rh7CG081500 Rh7DG082400
rosa_wichuraiana Rw7G006840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 487
AfaI GTAC 2 cut(s) 122, 146
AfiI CCNNNNNNNGG 2 cut(s) 50, 497
AflIII ACRYGT 1 cut(s) 339
AgsI TTSAA 6 cut(s) 140, 309, 406, 544, 607, 637
AloI GAACNNNNNNTCC 2 cut(s) 617, 649
AluBI AGCT 3 cut(s) 191, 283, 610
AluI AGCT 3 cut(s) 191, 283, 610
Alw26I GTCTC 4 cut(s) 15, 273, 366, 523
Ama87I CYCGRG 1 cut(s) 205
AoxI GGCC 2 cut(s) 195, 615
ApeKI GCWGC 2 cut(s) 387, 470
Asp700I GAANNNNTTC 1 cut(s) 447
AspS9I GGNCC 1 cut(s) 615
AsuHPI GGTGA 2 cut(s) 512, 633
AvaI CYCGRG 1 cut(s) 205
BbsI GAAGAC 1 cut(s) 440
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 374, 457
BccI CCATC 2 cut(s) 311, 409
BceAI ACGGC 2 cut(s) 103, 313
BciVI GTATCC 2 cut(s) 314, 435
BcoDI GTCTC 4 cut(s) 15, 273, 366, 523
BfaI CTAG 4 cut(s) 236, 348, 474, 584
BfuI GTATCC 2 cut(s) 314, 435
BisI GCNGC 2 cut(s) 388, 471
BlsI GCNGC 2 cut(s) 389, 472
BmeT110I CYCGRG 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 615
BmsI GCATC 1 cut(s) 450
BpiI GAAGAC 1 cut(s) 440
Bpu10I CCTNAGC 2 cut(s) 192, 611
BsaAI YACGTR 1 cut(s) 574
BsaJI CCNNGG 1 cut(s) 492
BsaXI ACNNNNNCTCC 2 cut(s) 617, 647
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 497
Bse1I ACTGG 1 cut(s) 432
Bse3DI GCAATG 1 cut(s) 529
BseDI CCNNGG 1 cut(s) 492
BseGI GGATG 2 cut(s) 322, 512
BseLI CCNNNNNNNGG 2 cut(s) 50, 497
BseMI GCAATG 1 cut(s) 529
BseMII CTCAG 3 cut(s) 183, 278, 625
BseNI ACTGG 1 cut(s) 432
BseRI GAGGAG 1 cut(s) 641
BseXI GCAGC 2 cut(s) 374, 457
BshFI GGCC 2 cut(s) 197, 617
BsiHKCI CYCGRG 1 cut(s) 205
BsiWI CGTACG 1 cut(s) 120
BslI CCNNNNNNNGG 2 cut(s) 50, 497
BsmAI GTCTC 4 cut(s) 15, 273, 366, 523
BsmBI CGTCTC 1 cut(s) 15
BsmI GAATGC 1 cut(s) 45
BsnI GGCC 2 cut(s) 197, 617
BsoBI CYCGRG 1 cut(s) 205
BspANI GGCC 2 cut(s) 197, 617
BspCNI CTCAG 3 cut(s) 184, 277, 624
BsrDI GCAATG 1 cut(s) 529
BsrI ACTGG 1 cut(s) 432
BssECI CCNNGG 1 cut(s) 492
BssNAI GTATAC 1 cut(s) 488
Bst1107I GTATAC 1 cut(s) 488
Bst4CI ACNGT 1 cut(s) 125
BstBAI YACGTR 1 cut(s) 574
BstC8I GCNNGC 3 cut(s) 185, 189, 292
BstDEI CTNAG 4 cut(s) 192, 264, 365, 611
BstDSI CCRYGG 1 cut(s) 492
BstF5I GGATG 2 cut(s) 322, 512
BstMAI GTCTC 4 cut(s) 15, 273, 366, 523
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstNSI RCATGY 1 cut(s) 343
BstSNI TACGTA 1 cut(s) 574
BstV1I GCAGC 2 cut(s) 374, 457
BstV2I GAAGAC 1 cut(s) 440
BstZ17I GTATAC 1 cut(s) 488
BsuI GTATCC 2 cut(s) 314, 435
BsuRI GGCC 2 cut(s) 197, 617
BtgI CCRYGG 1 cut(s) 492
BtsCI GGATG 2 cut(s) 322, 512
Cac8I GCNNGC 3 cut(s) 185, 189, 292
Cfr13I GGNCC 1 cut(s) 615
Csp6I GTAC 2 cut(s) 121, 145
CviAII CATG 3 cut(s) 272, 340, 371
CviJI RGCY 9 cut(s) 191, 197, 283, 294, 470, 610, 617, 656, 678
CviKI_1 RGCY 9 cut(s) 191, 197, 283, 294, 470, 610, 617, 656, 678
CviQI GTAC 2 cut(s) 121, 145
DdeI CTNAG 4 cut(s) 192, 264, 365, 611
Eco105I TACGTA 1 cut(s) 574
Eco88I CYCGRG 1 cut(s) 205
Esp3I CGTCTC 1 cut(s) 15
FaeI CATG 3 cut(s) 275, 343, 374
FatI CATG 3 cut(s) 271, 339, 370
FblI GTMKAC 1 cut(s) 487
Fnu4HI GCNGC 2 cut(s) 388, 471
FokI GGATG 2 cut(s) 329, 499
Fsp4HI GCNGC 2 cut(s) 388, 471
FspBI CTAG 4 cut(s) 236, 348, 474, 584
GluI GCNGC 2 cut(s) 388, 471
HaeIII GGCC 2 cut(s) 197, 617
Hin1II CATG 3 cut(s) 275, 343, 374
HinfI GANTC 2 cut(s) 104, 531
HphI GGTGA 2 cut(s) 512, 633
Hpy166II GTNNAC 2 cut(s) 488, 663
Hpy188I TCNGA 2 cut(s) 38, 280
Hpy188III TCNNGA 4 cut(s) 150, 207, 440, 579
Hpy8I GTNNAC 2 cut(s) 488, 663
HpyAV CCTTC 3 cut(s) 437, 491, 607
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4IV ACGT 1 cut(s) 573
HpyCH4V TGCA 5 cut(s) 71, 82, 128, 166, 522
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 4 cut(s) 192, 264, 365, 611
HpySE526I ACGT 1 cut(s) 573
Hsp92II CATG 3 cut(s) 275, 343, 374
LmnI GCTCC 2 cut(s) 280, 395
Lsp1109I GCAGC 2 cut(s) 374, 457
LweI GCATC 1 cut(s) 450
MaeI CTAG 4 cut(s) 236, 348, 474, 584
MaeII ACGT 1 cut(s) 573
MaeIII GTNAC 2 cut(s) 217, 553
MboII GAAGA 1 cut(s) 440
MfeI CAATTG 1 cut(s) 77
MluCI AATT 3 cut(s) 77, 83, 637
MlyI GAGTC 1 cut(s) 525
MmeI TCCRAC 1 cut(s) 16
MnlI CCTC 5 cut(s) 25, 187, 269, 604, 619
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 1 cut(s) 700
MunI CAATTG 1 cut(s) 77
Mva1269I GAATGC 1 cut(s) 45
MwoI GCNNNNNNNGC 1 cut(s) 236
NlaIII CATG 3 cut(s) 275, 343, 374
NspI RCATGY 1 cut(s) 343
PciI ACATGT 1 cut(s) 339
PctI GAATGC 1 cut(s) 45
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 1 cut(s) 104
Pfl23II CGTACG 1 cut(s) 120
PkrI GCNGC 2 cut(s) 389, 472
PleI GAGTC 1 cut(s) 525
PpsI GAGTC 1 cut(s) 525
Ppu21I YACGTR 1 cut(s) 574
PscI ACATGT 1 cut(s) 339
PspLI CGTACG 1 cut(s) 120
PspPI GGNCC 1 cut(s) 615
PsrI GAACNNNNNNTAC 2 cut(s) 202, 234
RsaI GTAC 2 cut(s) 122, 146
RsaNI GTAC 2 cut(s) 121, 145
SaqAI TTAA 1 cut(s) 700
SatI GCNGC 2 cut(s) 388, 471
Sau96I GGNCC 1 cut(s) 615
SchI GAGTC 1 cut(s) 525
SfaNI GCATC 1 cut(s) 450
SnaBI TACGTA 1 cut(s) 574
Sse9I AATT 3 cut(s) 77, 83, 637
SspI AATATT 1 cut(s) 650
SspMI CTAG 4 cut(s) 236, 348, 474, 584
TaaI ACNGT 1 cut(s) 125
TaiI ACGT 1 cut(s) 576
TasI AATT 3 cut(s) 77, 83, 637
TfiI GAWTC 1 cut(s) 104
Tru1I TTAA 1 cut(s) 700
Tru9I TTAA 1 cut(s) 700
TseI GCWGC 2 cut(s) 387, 470
TspGWI ACGGA 1 cut(s) 183
XceI RCATGY 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 487
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 4 cut(s) 236, 348, 474, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.