Rroxscaffold_4G00294340

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0015691
Physical Location & Seq
Forward (+)
14441061 .. 14452616
11556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00294340.1

Sequence Viewer

Length: 1806 bp
ATGGGTAGTTGGACAGACATGAAGGGATTCACCCTCCAAGTTCTTACAGGAAGGTGGCTCAAGTTGTTTGCCTCATTTCTAATGATGGCTGGTGCTGGTGCAAGCTACAGCTTCGGCCTCTACTCAAACGACATCAAGTCCTCGCTCGGTTACACCCAAACGACGCTCAATCTCATCAGCTTCTTCAAGGACTTGGGTGCCAACATTGGCATATTTTCGGGGCTAATCAACGAGCTCACTCCCCCATGGGTGGTCTTATCAATCGGTGCAGCTTTCAACTTCTTTGGCTACTTCATGATTTGGCTTGCCGTGACCAAAAAGATAGCCAAGCCCCAAGTTTGGCACATGTGCATGTACATAACAATGGGGGCAAACTCGCACACTTTCATCAACGCAGGAGCTCTAGGCACATGCGTGAAGAACTTTCCCAGAAGCCGGGGACTCTTGTTAGGCCTTTTGCAGGGCTACACTGGTCTAAGTGCAGCAGTAATAGCACAGCTATACCATGCTTTTTATGGTGATGACACTAAATCCTTCACATTGTTCGTCGCATGGCTTCCTTCTACTCTTACTTTGATATTTCTCCGAACCATTAGAATCATGCCGGTTATACCAAGCAAAAATGACCGCAATGTGTTACATAAATTCTTTTTTATCTCACTTGGACTTGCCATGTTCTTGTTGGTAATAATCATAGTAGAGCGGCAAGTTAACTTCACACAAAGTGAGTACGCTGGGAGTGCTGCTGTAGTGGTTTTCTTGCTCTTTCTCCCACTCGCTGTTGTTGTAGCCGTAGAGCAAAATATTTGGAGGAACAATCGACTAACTAGTACGGCAGAACATATCGATTCATCAGGCCAAAACCAGCTGAGTATAATTTCGTCCCCAGTATCATTAGCGTTACCCAATGATGACAAGACAATTTCTTGTTGGAATTGGAATCACATATTTAGTCCGCCACAGATAGGAAACGACTACACCATACTCCAAGCAGTGTTCAGCATTGAAATGTTAGCTCTGTTCTTGACAACACTATGCGGCCTAGGCGGCATGTTAACAATGATAGACAACTTGGGTCAGATTGGAACAGCATTAGGATACTCATTGGGAAGTGTAACCACATTTGTGTCACTCACAAGCATATGGTGTTACCTAGGGGAAGTAATGGTGGGTATGCTCTCAGATATCTTCGTCACAAAGTACAAATGTCCTAGACCTCTCATGTTCACTCTAACCCTCTTGCTATCATGCATTGGTCACCTTCTGATAGCGTTCAATGTCCCCAACGGTCTCTATGCGGCTGCGATAATCATTGGGTTTTGCTTCGGTGGACAGTGGCCACTGATATTTTCAGTAACTTCTGAGATTTTCGGGCTTAAGCACTGCTCTACTCTGAACAATATTGGGGTAATGGCTTGCCCACTGGGGTCAAATTTGCTCAATGTGAGGGTTACAGGGTTTCTCTATGATAAGGAGGCAGAGAAGCAACTGAAAGCTTTGGGATTGAAGGGGAAGATTGGGGAGGGGTTAAATTGTAGTGGGGTACAATGTTACAAATTGCCCTTCATTATAATCACTGGAGCGACAATGCTTGGTGTGCTTTTTTCACTTGTTTTGGTACTTAGGACTAGCAAGTTCTATAGCACTGACTTGTATAACAAGTTCAAAGATGAGGCCAAAGCAGCAGAGTATGAAACTGTGGTGGCCAGAAACCCAGTTGGACTAGAAAAATCTAGTGAAAAGTTAGAGTTGAGACTTGAGAGTGATTCAGCAAGTAGCAGAAGAAACCAACCTCAAGACGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 Nodulin-like

Protein Analysis

601

Amino Acids

66.08

Weight (kDa)

8.77

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 18 - 263 1.1e-84 Nodulin-like
NFD4_C PF23262 343 - 548 8.2e-17 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015691)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18460 FvH4_7g18460
malus_domestica MD01G1101300.v1.1
prunus_persica Prupe.2G206500_v2.0.a1
pyrus_communis pycom01g12790
rosa_chinensis RchiOBHm_Chr1g0362271
rosa_laevigata RLG00000027660
rosa_multiflora Rmu_sc0001237.1_g000019 Rmu_ssc0000009.1_g000005
rosa_roxburghii Rroxscaffold_4G00294320 Rroxscaffold_4G00294340
rosa_rugosa Rorug01G0296500
rosa_samantha Rh1AG305500 Rh1BG268700 Rh1CG286700 Rh1DG298800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1571
AatII GACGTC 1 cut(s) 1803
AccB1I GGYRCC 1 cut(s) 197
AccBSI CCGCTC 1 cut(s) 703
AciI CCGC 6 cut(s) 628, 703, 956, 1038, 1047, 1298
AcoI YGGCCR 2 cut(s) 1337, 1704
AcsI RAATTY 2 cut(s) 644, 1432
AcyI GRCGYC 1 cut(s) 1800
AdeI CACNNNGTG 1 cut(s) 725
AfaI GTAC 6 cut(s) 356, 731, 832, 1202, 1545, 1620
AfiI CCNNNNNNNGG 5 cut(s) 250, 339, 435, 460, 965
AflII CTTAAG 1 cut(s) 1376
AflIII ACRYGT 1 cut(s) 345
AgsI TTSAA 6 cut(s) 187, 277, 1007, 1276, 1507, 1666
AhdI GACNNNNNGTC 1 cut(s) 136
AhlI ACTAGT 1 cut(s) 827
AleI CACNNNNGTG 2 cut(s) 413, 1124
Alw21I GWGCWC 2 cut(s) 237, 403
Alw26I GTCTC 2 cut(s) 1295, 1747
AoxI GGCC 7 cut(s) 115, 451, 856, 1039, 1337, 1674, 1704
ApeKI GCWGC 5 cut(s) 269, 482, 743, 1301, 1682
ApoI RAATTY 2 cut(s) 644, 1432
Asp700I GAANNNNTTC 1 cut(s) 26
AspA2I CCTAGG 2 cut(s) 1042, 1153
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 3 cut(s) 22, 530, 1250
AvrII CCTAGG 2 cut(s) 1042, 1153
BalI TGGCCA 2 cut(s) 1339, 1706
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 2 cut(s) 237, 403
Bbv12I GWGCWC 2 cut(s) 237, 403
BbvI GCAGC 5 cut(s) 281, 494, 730, 1288, 1694
BccI CCATC 1 cut(s) 79
BceAI ACGGC 3 cut(s) 293, 776, 849
BciVI GTATCC 1 cut(s) 1091
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 2 cut(s) 1295, 1747
BcuI ACTAGT 1 cut(s) 827
BfaI CTAG 8 cut(s) 404, 828, 1043, 1154, 1212, 1629, 1724, 1734
BfmI CTRYAG 3 cut(s) 106, 747, 1639
BfrI CTTAAG 1 cut(s) 1376
BfuI GTATCC 1 cut(s) 1091
BglI GCCNNNNNGGC 1 cut(s) 1047
BisI GCNGC 9 cut(s) 270, 483, 704, 744, 1039, 1048, 1299, 1302, 1683
BlnI CCTAGG 2 cut(s) 1042, 1153
BlsI GCNGC 9 cut(s) 271, 484, 705, 745, 1040, 1049, 1300, 1303, 1684
Bme1390I CCNGG 1 cut(s) 437
BmeRI GACNNNNNGTC 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 199
BmrFI CCNGG 1 cut(s) 437
BmrI ACTGGG 3 cut(s) 881, 1433, 1709
BmuI ACTGGG 3 cut(s) 881, 1433, 1709
BpmI CTGGAG 1 cut(s) 1599
BpuEI CTTGAG 3 cut(s) 44, 1778, 1779
BpuMI CCSGG 1 cut(s) 437
Bsa29I ATCGAT 1 cut(s) 846
BsaHI GRCGYC 1 cut(s) 1800
BsaI GGTCTC 1 cut(s) 1295
BsaJI CCNNGG 4 cut(s) 245, 436, 1042, 1153
Bsc4I CCNNNNNNNGG 5 cut(s) 250, 339, 435, 460, 965
Bse118I RCCGGY 1 cut(s) 604
Bse1I ACTGG 5 cut(s) 475, 887, 1428, 1582, 1715
Bse3DI GCAATG 1 cut(s) 637
BseCI ATCGAT 1 cut(s) 846
BseDI CCNNGG 4 cut(s) 245, 436, 1042, 1153
BseLI CCNNNNNNNGG 5 cut(s) 250, 339, 435, 460, 965
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 3 cut(s) 860, 1194, 1353
BseNI ACTGG 5 cut(s) 475, 887, 1428, 1582, 1715
BseXI GCAGC 5 cut(s) 281, 494, 730, 1288, 1694
BseYI CCCAGC 1 cut(s) 734
BsgI GTGCAG 2 cut(s) 288, 501
BshFI GGCC 7 cut(s) 117, 453, 858, 1041, 1339, 1676, 1706
BshNI GGYRCC 1 cut(s) 197
BshVI ATCGAT 1 cut(s) 846
BsiHKAI GWGCWC 2 cut(s) 237, 403
BsiSI CCGG 2 cut(s) 436, 605
BslFI GGGAC 3 cut(s) 453, 868, 1265
BslI CCNNNNNNNGG 5 cut(s) 250, 339, 435, 460, 965
BsmAI GTCTC 2 cut(s) 1295, 1747
BsmFI GGGAC 3 cut(s) 453, 868, 1265
BsnI GGCC 7 cut(s) 117, 453, 858, 1041, 1339, 1676, 1706
Bso31I GGTCTC 1 cut(s) 1295
Bsp1286I GDGCHC 2 cut(s) 237, 403
Bsp1407I TGTACA 1 cut(s) 354
Bsp19I CCATGG 1 cut(s) 245
BspACI CCGC 6 cut(s) 628, 703, 956, 1038, 1047, 1298
BspANI GGCC 7 cut(s) 117, 453, 858, 1041, 1339, 1676, 1706
BspCNI CTCAG 3 cut(s) 861, 1193, 1354
BspDI ATCGAT 1 cut(s) 846
BspHI TCATGA 1 cut(s) 294
BspLI GGNNCC 1 cut(s) 199
BspT107I GGYRCC 1 cut(s) 197
BspTI CTTAAG 1 cut(s) 1376
BspTNI GGTCTC 1 cut(s) 1295
BsrBI CCGCTC 1 cut(s) 703
BsrDI GCAATG 1 cut(s) 637
BsrFI RCCGGY 1 cut(s) 604
BsrGI TGTACA 1 cut(s) 354
BsrI ACTGG 5 cut(s) 475, 887, 1428, 1582, 1715
BssAI RCCGGY 1 cut(s) 604
BssECI CCNNGG 4 cut(s) 245, 436, 1042, 1153
BssNI GRCGYC 1 cut(s) 1800
BssT1I CCWWGG 3 cut(s) 245, 1042, 1153
Bst4CI ACNGT 3 cut(s) 1289, 1335, 1699
BstACI GRCGYC 1 cut(s) 1800
BstAFI CTTAAG 1 cut(s) 1376
BstAUI TGTACA 1 cut(s) 354
BstC8I GCNNGC 3 cut(s) 103, 306, 1417
BstDEI CTNAG 5 cut(s) 476, 869, 1180, 1362, 1622
BstDSI CCRYGG 1 cut(s) 245
BstEII GGTNACC 1 cut(s) 1256
BstENI CCTNNNNNAGG 1 cut(s) 458
BstMAI GTCTC 2 cut(s) 1295, 1747
BstMWI GCNNNNNNNGC 6 cut(s) 491, 740, 1044, 1047, 1597, 1682
BstNSI RCATGY 4 cut(s) 349, 355, 414, 1054
BstPI GGTNACC 1 cut(s) 1256
BstSCI CCNGG 1 cut(s) 435
BstSFI CTRYAG 3 cut(s) 106, 747, 1639
BstV1I GCAGC 5 cut(s) 281, 494, 730, 1288, 1694
Bsu15I ATCGAT 1 cut(s) 846
BsuI GTATCC 1 cut(s) 1091
BsuRI GGCC 7 cut(s) 117, 453, 858, 1041, 1339, 1676, 1706
BsuTUI ATCGAT 1 cut(s) 846
BtgI CCRYGG 1 cut(s) 245
BtsI GCAGTG 2 cut(s) 999, 1381
BtsIMutI CAGTG 8 cut(s) 468, 999, 1340, 1340, 1381, 1421, 1575, 1644
Cac8I GCNNGC 3 cut(s) 103, 306, 1417
CciI TCATGA 1 cut(s) 294
Cfr10I RCCGGY 1 cut(s) 604
ClaI ATCGAT 1 cut(s) 846
CseI GACGC 1 cut(s) 172
Csp6I GTAC 6 cut(s) 355, 730, 831, 1201, 1544, 1619
CviQI GTAC 6 cut(s) 355, 730, 831, 1201, 1544, 1619
DdeI CTNAG 5 cut(s) 476, 869, 1180, 1362, 1622
DraIII CACNNNGTG 1 cut(s) 725
DriI GACNNNNNGTC 1 cut(s) 136
EaeI YGGCCR 2 cut(s) 1337, 1704
Eam1105I GACNNNNNGTC 1 cut(s) 136
EciI GGCGGA 1 cut(s) 945
Ecl136II GAGCTC 2 cut(s) 235, 401
Eco130I CCWWGG 3 cut(s) 245, 1042, 1153
Eco147I AGGCCT 1 cut(s) 453
Eco24I GRGCYC 2 cut(s) 237, 403
Eco31I GGTCTC 1 cut(s) 1295
Eco32I GATATC 1 cut(s) 1186
Eco53kI GAGCTC 2 cut(s) 235, 401
Eco91I GGTNACC 1 cut(s) 1256
EcoICRI GAGCTC 2 cut(s) 235, 401
EcoNI CCTNNNNNAGG 1 cut(s) 458
EcoO65I GGTNACC 1 cut(s) 1256
EcoRV GATATC 1 cut(s) 1186
EcoT14I CCWWGG 3 cut(s) 245, 1042, 1153
EcoT22I ATGCAT 1 cut(s) 1253
EcoT38I GRGCYC 2 cut(s) 237, 403
ErhI CCWWGG 3 cut(s) 245, 1042, 1153
FaqI GGGAC 3 cut(s) 453, 868, 1265
FauNDI CATATG 1 cut(s) 1142
Fnu4HI GCNGC 9 cut(s) 270, 483, 704, 744, 1039, 1048, 1299, 1302, 1683
FriOI GRGCYC 2 cut(s) 237, 403
Fsp4HI GCNGC 9 cut(s) 270, 483, 704, 744, 1039, 1048, 1299, 1302, 1683
FspBI CTAG 8 cut(s) 404, 828, 1043, 1154, 1212, 1629, 1724, 1734
GluI GCNGC 9 cut(s) 270, 483, 704, 744, 1039, 1048, 1299, 1302, 1683
GsaI CCCAGC 1 cut(s) 738
GsuI CTGGAG 1 cut(s) 1599
HaeIII GGCC 7 cut(s) 117, 453, 858, 1041, 1339, 1676, 1706
HapII CCGG 2 cut(s) 436, 605
HgaI GACGC 1 cut(s) 172
Hin1I GRCGYC 1 cut(s) 1800
HincII GTYRAC 2 cut(s) 712, 1056
HindII GTYRAC 2 cut(s) 712, 1056
HindIII AAGCTT 1 cut(s) 1494
HinfI GANTC 6 cut(s) 27, 441, 597, 848, 940, 1766
HpaI GTTAAC 2 cut(s) 712, 1056
HpaII CCGG 2 cut(s) 436, 605
HphI GGTGA 3 cut(s) 22, 530, 1250
Hpy166II GTNNAC 4 cut(s) 712, 1056, 1227, 1331
Hpy188I TCNGA 7 cut(s) 587, 1080, 1183, 1266, 1363, 1395, 1805
Hpy188III TCNNGA 3 cut(s) 295, 1024, 1796
Hpy8I GTNNAC 4 cut(s) 712, 1056, 1227, 1331
Hpy99I CGWCG 2 cut(s) 166, 551
HpyAV CCTTC 7 cut(s) 16, 45, 544, 570, 1271, 1501, 1573
HpyCH4III ACNGT 3 cut(s) 1289, 1335, 1699
HpyCH4IV ACGT 1 cut(s) 1800
HpyCH4V TGCA 6 cut(s) 101, 269, 351, 460, 482, 1251
HpyF10VI GCNNNNNNNGC 6 cut(s) 491, 740, 1044, 1047, 1597, 1682
HpyF3I CTNAG 5 cut(s) 476, 869, 1180, 1362, 1622
HpySE526I ACGT 1 cut(s) 1800
Hsp92I GRCGYC 1 cut(s) 1800
KspAI GTTAAC 2 cut(s) 712, 1056
LmnI GCTCC 2 cut(s) 398, 1580
Lsp1109I GCAGC 5 cut(s) 281, 494, 730, 1288, 1694
MaeI CTAG 8 cut(s) 404, 828, 1043, 1154, 1212, 1629, 1724, 1734
MaeII ACGT 1 cut(s) 1800
MbiI CCGCTC 1 cut(s) 703
MboII GAAGA 5 cut(s) 175, 430, 1180, 1525, 1794
MhlI GDGCHC 2 cut(s) 237, 403
MlsI TGGCCA 2 cut(s) 1339, 1706
MluCI AATT 7 cut(s) 644, 876, 921, 934, 1432, 1531, 1556
MluNI TGGCCA 2 cut(s) 1339, 1706
MlyI GAGTC 1 cut(s) 435
MmeI TCCRAC 2 cut(s) 911, 1699
Mox20I TGGCCA 2 cut(s) 1339, 1706
Mph1103I ATGCAT 1 cut(s) 1253
MroXI GAANNNNTTC 1 cut(s) 26
MscI TGGCCA 2 cut(s) 1339, 1706
MseI TTAA 4 cut(s) 711, 1055, 1377, 1529
MslI CAYNNNNRTG 5 cut(s) 350, 362, 413, 1007, 1124
Msp20I TGGCCA 2 cut(s) 1339, 1706
MspA1I CMGCKG 1 cut(s) 868
MspCI CTTAAG 1 cut(s) 1376
MspI CCGG 2 cut(s) 436, 605
MspR9I CCNGG 1 cut(s) 437
MwoI GCNNNNNNNGC 6 cut(s) 491, 740, 1044, 1047, 1597, 1682
NciI CCSGG 1 cut(s) 437
NcoI CCATGG 1 cut(s) 245
NdeI CATATG 1 cut(s) 1142
NlaIV GGNNCC 1 cut(s) 199
NmuCI GTSAC 4 cut(s) 310, 1128, 1192, 1256
NsiI ATGCAT 1 cut(s) 1253
NspI RCATGY 4 cut(s) 349, 355, 414, 1054
OliI CACNNNNGTG 2 cut(s) 413, 1124
PagI TCATGA 1 cut(s) 294
PceI AGGCCT 1 cut(s) 453
PciI ACATGT 1 cut(s) 345
PdmI GAANNNNTTC 1 cut(s) 26
PfeI GAWTC 5 cut(s) 27, 597, 848, 940, 1766
PkrI GCNGC 9 cut(s) 271, 484, 705, 745, 1040, 1049, 1300, 1303, 1684
PleI GAGTC 1 cut(s) 435
PpsI GAGTC 1 cut(s) 435
PscI ACATGT 1 cut(s) 345
PsiI TTATAA 1 cut(s) 1571
Psp124BI GAGCTC 2 cut(s) 237, 403
PspEI GGTNACC 1 cut(s) 1256
PspFI CCCAGC 1 cut(s) 734
PspN4I GGNNCC 1 cut(s) 199
PsrI GAACNNNNNNTAC 2 cut(s) 1646, 1678
PvuII CAGCTG 1 cut(s) 868
RsaI GTAC 6 cut(s) 356, 731, 832, 1202, 1545, 1620
RsaNI GTAC 6 cut(s) 355, 730, 831, 1201, 1544, 1619
RseI CAYNNNNRTG 5 cut(s) 350, 362, 413, 1007, 1124
SacI GAGCTC 2 cut(s) 237, 403
SaqAI TTAA 4 cut(s) 711, 1055, 1377, 1529
SatI GCNGC 9 cut(s) 270, 483, 704, 744, 1039, 1048, 1299, 1302, 1683
SchI GAGTC 1 cut(s) 435
ScrFI CCNGG 1 cut(s) 437
SduI GDGCHC 2 cut(s) 237, 403
SfcI CTRYAG 3 cut(s) 106, 747, 1639
SmiMI CAYNNNNRTG 5 cut(s) 350, 362, 413, 1007, 1124
SmlI CTYRAG 4 cut(s) 59, 1376, 1757, 1794
SmoI CTYRAG 4 cut(s) 59, 1376, 1757, 1794
SpeI ACTAGT 1 cut(s) 827
Sse9I AATT 7 cut(s) 644, 876, 921, 934, 1432, 1531, 1556
SseBI AGGCCT 1 cut(s) 453
SsiI CCGC 6 cut(s) 628, 703, 956, 1038, 1047, 1298
SspI AATATT 2 cut(s) 805, 1402
SspMI CTAG 8 cut(s) 404, 828, 1043, 1154, 1212, 1629, 1724, 1734
SstI GAGCTC 2 cut(s) 237, 403
StuI AGGCCT 1 cut(s) 453
StyD4I CCNGG 1 cut(s) 435
StyI CCWWGG 3 cut(s) 245, 1042, 1153
TaaI ACNGT 3 cut(s) 1289, 1335, 1699
TaiI ACGT 1 cut(s) 1803
TaqI TCGA 2 cut(s) 820, 846
TasI AATT 7 cut(s) 644, 876, 921, 934, 1432, 1531, 1556
TatI WGTACW 2 cut(s) 354, 1200
TauI GCSGC 4 cut(s) 706, 1041, 1050, 1301
TfiI GAWTC 5 cut(s) 27, 597, 848, 940, 1766
Tru1I TTAA 4 cut(s) 711, 1055, 1377, 1529
Tru9I TTAA 4 cut(s) 711, 1055, 1377, 1529
TscAI CASTG 8 cut(s) 475, 999, 1340, 1347, 1388, 1428, 1582, 1651
TseFI GTSAC 4 cut(s) 310, 1128, 1192, 1256
TseI GCWGC 5 cut(s) 269, 482, 743, 1301, 1682
Tsp45I GTSAC 4 cut(s) 310, 1128, 1192, 1256
TspDTI ATGAA 6 cut(s) 35, 283, 376, 840, 1555, 1707
TspRI CASTG 8 cut(s) 475, 999, 1340, 1347, 1388, 1428, 1582, 1651
Vha464I CTTAAG 1 cut(s) 1376
XagI CCTNNNNNAGG 1 cut(s) 458
XapI RAATTY 2 cut(s) 644, 1432
XceI RCATGY 4 cut(s) 349, 355, 414, 1054
XcmI CCANNNNNNNNNTGG 2 cut(s) 512, 679
XmaJI CCTAGG 2 cut(s) 1042, 1153
XmnI GAANNNNTTC 1 cut(s) 26
XspI CTAG 8 cut(s) 404, 828, 1043, 1154, 1212, 1629, 1724, 1734
ZraI GACGTC 1 cut(s) 1801
Zsp2I ATGCAT 1 cut(s) 1253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.