Rroxscaffold_4G00312560

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0022972
Physical Location & Seq
Reverse (-)
35598430 .. 35598879
450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00312560.1

Sequence Viewer

Length: 450 bp
ATGCGCGCAATAGAGTATGCACCGTATGCTTATGGTCTTCCATTACTTTTGGCAGTTTTAGGTAGTCATCTACGTGGTAGAAGTATAGATGAATGGAAAGAGACTTTGCATTCCAAAAGAGCCCATTACATCGAGTTTGAATATGTGCTTAGACGAAGCTATGATGCATTGAACGATACCGTGAAAATAGCCTTCCTTGACATTGCATGTTTCTTCAAAGGTAAAAAGATGAAATATGTACAACAAATTCTAGAAGATTCTGGACTAGACTCTAGAATCGCTATTCAAACACTTGCAGAAAAGGCCCTTATAACTATTTCAAATCAGCCTTTGGTTGGTAATATTGTTTCGATGCATGATTTGATAGAGGAAATGGGAACAGATATAGTTCACCAAGAATCACCAAACGAACCGGAGAGCGGAGCGATTGTGGTGGCACGTGGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

149

Amino Acids

16.78

Weight (kDa)

5.71

Isoelectric Point (pI)

63.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 62 - 134 6e-20 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022972)

Species Orthologous Gene IDs
rosa_laevigata RLG00000029108
rosa_multiflora Rmu_sc0001400.1_g000003
rosa_roxburghii Rroxscaffold_4G00312560
rosa_wichuraiana Rw1G014080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 311
AccBSI CCGCTC 1 cut(s) 420
AccII CGCG 1 cut(s) 6
AciI CCGC 1 cut(s) 420
AcsI RAATTY 1 cut(s) 246
AcvI CACGTG 1 cut(s) 440
AfaI GTAC 1 cut(s) 240
AfiI CCNNNNNNNGG 2 cut(s) 335, 419
AgsI TTSAA 5 cut(s) 140, 172, 217, 287, 321
AluBI AGCT 1 cut(s) 159
AluI AGCT 1 cut(s) 159
Alw26I GTCTC 1 cut(s) 95
AoxI GGCC 1 cut(s) 303
ApoI RAATTY 1 cut(s) 246
AspLEI GCGC 2 cut(s) 6, 8
AspS9I GGNCC 1 cut(s) 304
AsuHPI GGTGA 2 cut(s) 383, 393
BanII GRGCYC 1 cut(s) 124
BbrPI CACGTG 1 cut(s) 440
BbsI GAAGAC 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 3 cut(s) 251, 266, 273
BmgT120I GGNCC 1 cut(s) 304
BmsI GCATC 2 cut(s) 154, 342
BpiI GAAGAC 1 cut(s) 29
BsaAI YACGTR 2 cut(s) 74, 440
BsaWI WCCGGW 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 414, 444
Bsc4I CCNNNNNNNGG 2 cut(s) 335, 419
Bse3DI GCAATG 1 cut(s) 201
BseGI GGATG 1 cut(s) 449
BseLI CCNNNNNNNGG 2 cut(s) 335, 419
BseMI GCAATG 1 cut(s) 201
BsePI GCGCGC 1 cut(s) 4
Bsh1236I CGCG 1 cut(s) 6
BshFI GGCC 1 cut(s) 305
BsiSI CCGG 1 cut(s) 413
BslI CCNNNNNNNGG 2 cut(s) 335, 419
BsmAI GTCTC 1 cut(s) 95
BsmI GAATGC 1 cut(s) 109
BsnI GGCC 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 124
Bsp1407I TGTACA 1 cut(s) 238
BspACI CCGC 1 cut(s) 420
BspANI GGCC 1 cut(s) 305
BspFNI CGCG 1 cut(s) 6
BsrBI CCGCTC 1 cut(s) 420
BsrDI GCAATG 1 cut(s) 201
BsrGI TGTACA 1 cut(s) 238
BssHII GCGCGC 1 cut(s) 4
Bst4CI ACNGT 2 cut(s) 24, 181
BstAPI GCANNNNNTGC 1 cut(s) 26
BstAUI TGTACA 1 cut(s) 238
BstBAI YACGTR 2 cut(s) 74, 440
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 149
BstF5I GGATG 1 cut(s) 449
BstFNI CGCG 1 cut(s) 6
BstHHI GCGC 2 cut(s) 6, 8
BstMAI GTCTC 1 cut(s) 95
BstMWI GCNNNNNNNGC 2 cut(s) 26, 302
BstNSI RCATGY 1 cut(s) 210
BstUI CGCG 1 cut(s) 6
BstV2I GAAGAC 1 cut(s) 29
BsuRI GGCC 1 cut(s) 305
BtsCI GGATG 1 cut(s) 449
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 2 cut(s) 6, 8
Cfr13I GGNCC 1 cut(s) 304
Csp6I GTAC 1 cut(s) 239
CviAII CATG 2 cut(s) 207, 356
CviJI RGCY 5 cut(s) 122, 159, 191, 305, 328
CviKI_1 RGCY 5 cut(s) 122, 159, 191, 305, 328
CviQI GTAC 1 cut(s) 239
DdeI CTNAG 1 cut(s) 149
Eco24I GRGCYC 1 cut(s) 124
Eco72I CACGTG 1 cut(s) 440
EcoO109I RGGNCCY 1 cut(s) 304
EcoT22I ATGCAT 2 cut(s) 169, 357
EcoT38I GRGCYC 1 cut(s) 124
FaeI CATG 2 cut(s) 210, 359
FatI CATG 2 cut(s) 206, 355
FriOI GRGCYC 1 cut(s) 124
FspBI CTAG 3 cut(s) 251, 266, 273
GlaI GCGC 2 cut(s) 5, 7
HaeIII GGCC 1 cut(s) 305
HapII CCGG 1 cut(s) 413
HhaI GCGC 2 cut(s) 6, 8
Hin1II CATG 2 cut(s) 210, 359
Hin6I GCGC 2 cut(s) 4, 6
HinP1I GCGC 2 cut(s) 4, 6
HinfI GANTC 4 cut(s) 257, 269, 276, 398
HpaII CCGG 1 cut(s) 413
HphI GGTGA 2 cut(s) 383, 393
Hpy166II GTNNAC 1 cut(s) 391
Hpy188III TCNNGA 3 cut(s) 251, 261, 273
Hpy8I GTNNAC 1 cut(s) 391
HpyAV CCTTC 1 cut(s) 202
HpyCH4III ACNGT 2 cut(s) 24, 181
HpyCH4IV ACGT 2 cut(s) 73, 439
HpyCH4V TGCA 6 cut(s) 20, 109, 167, 206, 296, 355
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 302
HpyF3I CTNAG 1 cut(s) 149
HpySE526I ACGT 2 cut(s) 73, 439
Hsp92II CATG 2 cut(s) 210, 359
HspAI GCGC 2 cut(s) 4, 6
LmnI GCTCC 1 cut(s) 422
LpnPI CCDG 2 cut(s) 246, 426
LweI GCATC 2 cut(s) 154, 342
MaeI CTAG 3 cut(s) 251, 266, 273
MaeII ACGT 2 cut(s) 73, 439
MbiI CCGCTC 1 cut(s) 420
MboII GAAGA 3 cut(s) 29, 205, 266
MhlI GDGCHC 1 cut(s) 124
MluCI AATT 1 cut(s) 246
MlyI GAGTC 1 cut(s) 263
MnlI CCTC 1 cut(s) 361
Mph1103I ATGCAT 2 cut(s) 169, 357
MslI CAYNNNNRTG 2 cut(s) 72, 442
MspI CCGG 1 cut(s) 413
Mva1269I GAATGC 1 cut(s) 109
MvnI CGCG 1 cut(s) 6
MwoI GCNNNNNNNGC 2 cut(s) 26, 302
NlaIII CATG 2 cut(s) 210, 359
NsiI ATGCAT 2 cut(s) 169, 357
NspI RCATGY 1 cut(s) 210
PauI GCGCGC 1 cut(s) 4
PctI GAATGC 1 cut(s) 109
PfeI GAWTC 3 cut(s) 257, 276, 398
PleI GAGTC 1 cut(s) 263
PmaCI CACGTG 1 cut(s) 440
PmlI CACGTG 1 cut(s) 440
PpsI GAGTC 1 cut(s) 263
Ppu21I YACGTR 2 cut(s) 74, 440
PsiI TTATAA 1 cut(s) 311
PspCI CACGTG 1 cut(s) 440
PspPI GGNCC 1 cut(s) 304
PteI GCGCGC 1 cut(s) 4
RsaI GTAC 1 cut(s) 240
RsaNI GTAC 1 cut(s) 239
RseI CAYNNNNRTG 2 cut(s) 72, 442
Sau96I GGNCC 1 cut(s) 304
SchI GAGTC 1 cut(s) 263
SduI GDGCHC 1 cut(s) 124
SetI ASST 5 cut(s) 64, 76, 161, 223, 442
SfaNI GCATC 2 cut(s) 154, 342
SmiMI CAYNNNNRTG 2 cut(s) 72, 442
Sse9I AATT 1 cut(s) 246
SsiI CCGC 1 cut(s) 420
SspI AATATT 1 cut(s) 343
SspMI CTAG 3 cut(s) 251, 266, 273
TaaI ACNGT 2 cut(s) 24, 181
TaiI ACGT 2 cut(s) 76, 442
TaqI TCGA 2 cut(s) 132, 350
TasI AATT 1 cut(s) 246
TatI WGTACW 1 cut(s) 238
TfiI GAWTC 3 cut(s) 257, 276, 398
TspDTI ATGAA 2 cut(s) 105, 245
XapI RAATTY 1 cut(s) 246
XbaI TCTAGA 2 cut(s) 250, 272
XceI RCATGY 1 cut(s) 210
XspI CTAG 3 cut(s) 251, 266, 273
Zsp2I ATGCAT 2 cut(s) 169, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.