Rroxscaffold_5G00355980

Divergent PAP2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0018465
Physical Location & Seq
Reverse (-)
35126986 .. 35127504
519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00355980.1

Sequence Viewer

Length: 249 bp
ATGGCAGCGATTTCGGTGAACCTCACTCTCATCTCCTCCTTCCTCGCTTGCGCCGTCGCTCAGTTCCTCAAGCTTTGCACGTCCTGGTACAAAGATAGGAGATGGGATTCGAAACGAATACTGGATTCTGGAGGAATGTCGTCGTTGCATTGGGCGACTGTGACGGCGCTGATGGTGGCCGTTGGGCTAGACCAGGGTACAGAAGGATATGCTTTCGCTCTTGCTCAAATTACATATTACCCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF212

Protein Analysis

82

Amino Acids

9.01

Weight (kDa)

7.79

Isoelectric Point (pI)

55.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF212 PF02681 7 - 77 1.5e-18 Divergent PAP2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 177
AfaI GTAC 2 cut(s) 89, 199
AjiI CACGTC 1 cut(s) 81
AjnI CCWGG 2 cut(s) 83, 192
AluBI AGCT 1 cut(s) 73
AluI AGCT 1 cut(s) 73
AoxI GGCC 1 cut(s) 177
ApeKI GCWGC 1 cut(s) 5
AspLEI GCGC 2 cut(s) 53, 169
AsuHPI GGTGA 1 cut(s) 28
AsuII TTCGAA 1 cut(s) 110
BbvI GCAGC 1 cut(s) 17
BccI CCATC 2 cut(s) 96, 166
BceAI ACGGC 3 cut(s) 38, 164, 180
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 2 cut(s) 85, 194
BfaI CTAG 1 cut(s) 188
BfoI RGCGCY 1 cut(s) 170
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 85, 194
BmgBI CACGTC 1 cut(s) 81
BmrFI CCNGG 2 cut(s) 85, 194
BpmI CTGGAG 1 cut(s) 150
Bpu14I TTCGAA 1 cut(s) 110
BpuEI CTTGAG 1 cut(s) 53
BsaJI CCNNGG 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 126
BseBI CCWGG 2 cut(s) 85, 194
BseDI CCNNGG 1 cut(s) 193
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 1 cut(s) 126
BseRI GAGGAG 1 cut(s) 25
BseXI GCAGC 1 cut(s) 17
BshFI GGCC 1 cut(s) 179
BsnI GGCC 1 cut(s) 179
Bsp119I TTCGAA 1 cut(s) 110
BspANI GGCC 1 cut(s) 179
BspCNI CTCAG 1 cut(s) 73
BspT104I TTCGAA 1 cut(s) 110
BsrI ACTGG 1 cut(s) 126
BssECI CCNNGG 1 cut(s) 193
Bst2UI CCWGG 2 cut(s) 85, 194
Bst4CI ACNGT 1 cut(s) 160
BstBI TTCGAA 1 cut(s) 110
BstC8I GCNNGC 1 cut(s) 49
BstDEI CTNAG 1 cut(s) 60
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 2 cut(s) 53, 169
BstNI CCWGG 2 cut(s) 85, 194
BstSCI CCNGG 2 cut(s) 83, 192
BstV1I GCAGC 1 cut(s) 17
BsuRI GGCC 1 cut(s) 179
BtrI CACGTC 1 cut(s) 81
Cac8I GCNNGC 1 cut(s) 49
CfoI GCGC 2 cut(s) 53, 169
Csp6I GTAC 2 cut(s) 88, 198
CviJI RGCY 3 cut(s) 73, 179, 187
CviKI_1 RGCY 3 cut(s) 73, 179, 187
CviQI GTAC 2 cut(s) 88, 198
DdeI CTNAG 1 cut(s) 60
EaeI YGGCCR 1 cut(s) 177
EcoRII CCWGG 2 cut(s) 83, 192
FaiI YATR 2 cut(s) 210, 235
Fnu4HI GCNGC 1 cut(s) 6
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 188
GlaI GCGC 2 cut(s) 52, 168
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 150
HaeII RGCGCY 1 cut(s) 170
HaeIII GGCC 1 cut(s) 179
HhaI GCGC 2 cut(s) 53, 169
Hin6I GCGC 2 cut(s) 51, 167
HinP1I GCGC 2 cut(s) 51, 167
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 2 cut(s) 107, 125
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 1 cut(s) 19
Hpy188III TCNNGA 1 cut(s) 129
Hpy8I GTNNAC 1 cut(s) 19
Hpy99I CGWCG 2 cut(s) 59, 145
HpyAV CCTTC 2 cut(s) 49, 197
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4IV ACGT 1 cut(s) 80
HpyCH4V TGCA 2 cut(s) 78, 148
HpyF3I CTNAG 1 cut(s) 60
HpySE526I ACGT 1 cut(s) 80
HspAI GCGC 2 cut(s) 51, 167
LpnPI CCDG 6 cut(s) 70, 97, 107, 114, 179, 206
Lsp1109I GCAGC 1 cut(s) 17
MaeI CTAG 1 cut(s) 188
MaeII ACGT 1 cut(s) 80
MaeIII GTNAC 1 cut(s) 160
MluCI AATT 2 cut(s) 228, 244
MnlI CCTC 5 cut(s) 32, 46, 53, 77, 125
MseI TTAA 1 cut(s) 247
MspR9I CCNGG 2 cut(s) 85, 194
MvaI CCWGG 2 cut(s) 85, 194
NmuCI GTSAC 1 cut(s) 160
NspV TTCGAA 1 cut(s) 110
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PfeI GAWTC 2 cut(s) 107, 125
PkrI GCNGC 1 cut(s) 7
Psp6I CCWGG 2 cut(s) 83, 192
PspGI CCWGG 2 cut(s) 83, 192
RsaI GTAC 2 cut(s) 89, 199
RsaNI GTAC 2 cut(s) 88, 198
SaqAI TTAA 1 cut(s) 247
SatI GCNGC 1 cut(s) 6
ScrFI CCNGG 2 cut(s) 85, 194
SetI ASST 3 cut(s) 24, 75, 83
SfuI TTCGAA 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 2 cut(s) 228, 244
SspMI CTAG 1 cut(s) 188
StyD4I CCNGG 2 cut(s) 83, 192
TaaI ACNGT 1 cut(s) 160
TaiI ACGT 1 cut(s) 83
TaqI TCGA 1 cut(s) 110
TasI AATT 2 cut(s) 228, 244
TfiI GAWTC 2 cut(s) 107, 125
Tru1I TTAA 1 cut(s) 247
Tru9I TTAA 1 cut(s) 247
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 160
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.