Rroxscaffold_6G00390450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0021270
Physical Location & Seq
Forward (+)
5468500 .. 5471649
3150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00390450.1

Sequence Viewer

Length: 462 bp
ATGGTGCTGCAGAGAGTCCACCTCGGTGGTCGGAGATCGGTGACGGAGGAGAGAGATTCCTCCAGATCCACTTTCTCTCTCCTCCGTCCCTCTCATTCTGGGTTTCGTTGTGGTTTCTTTAATATGGTGGTCAATCTTGGTGGTTCTGTACGGAGAATAAATCAAGAATCGTGGAGGAACAACGATATGGGTAGGCTATTTCTTTTCTTGCTCCTTATAGACCAAACCAAACCATATTCCTTGCTCATCAAAACGAGGGTCGGCCGCCGGCGGGAGATTAATATGGCGGACTGCGCGAGGACTAAAGAAGCATTTGAAGATGCTCAATGCCCCCAAGTATTTTTGGCACGCTGGTTTGGGTTGAATCAGTCAAAATATCAGTGGGCATTTGATGATTGTTATGAAATCAAGGGATCGGGCATGTCTTGGAAATTGGATTGCATCGTGGAGGCCAAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

17.78

Weight (kDa)

9.61

Isoelectric Point (pI)

58.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021270)

Species Orthologous Gene IDs
pyrus_communis pycom07g14270
rosa_multiflora Rmu_sc0016337.1_g000004
rosa_roxburghii Rroxscaffold_6G00390450 Rroxscaffold_7G00186680
rosa_samantha Rh3CG356400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 296
AciI CCGC 3 cut(s) 265, 271, 287
AclWI GGATC 2 cut(s) 60, 421
AcoI YGGCCR 1 cut(s) 262
AfaI GTAC 1 cut(s) 150
AfiI CCNNNNNNNGG 1 cut(s) 271
AgsI TTSAA 2 cut(s) 317, 364
AleI CACNNNNGTG 1 cut(s) 24
AlwI GGATC 2 cut(s) 60, 421
AoxI GGCC 2 cut(s) 262, 450
ApeKI GCWGC 1 cut(s) 7
ArsI GACNNNNNNTTYG 2 cut(s) 243, 275
AseI ATTAAT 1 cut(s) 279
AspLEI GCGC 1 cut(s) 296
AsuHPI GGTGA 1 cut(s) 52
BfmI CTRYAG 1 cut(s) 8
BisI GCNGC 2 cut(s) 8, 265
BlsI GCNGC 2 cut(s) 9, 266
BmsI GCATC 2 cut(s) 310, 450
BplI GAGNNNNNCTC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 46
BsaJI CCNNGG 1 cut(s) 22
Bsc4I CCNNNNNNNGG 1 cut(s) 271
Bse118I RCCGGY 1 cut(s) 267
BseDI CCNNGG 1 cut(s) 22
BseLI CCNNNNNNNGG 1 cut(s) 271
BseRI GAGGAG 2 cut(s) 62, 71
BseX3I CGGCCG 1 cut(s) 262
Bsh1236I CGCG 1 cut(s) 296
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 2 cut(s) 264, 452
BsiEI CGRYCG 1 cut(s) 265
BsiSI CCGG 1 cut(s) 268
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 271
BsmFI GGGAC 1 cut(s) 72
BsnI GGCC 2 cut(s) 264, 452
Bsp143I GATC 3 cut(s) 35, 65, 413
BspACI CCGC 3 cut(s) 265, 271, 287
BspANI GGCC 2 cut(s) 264, 452
BspFNI CGCG 1 cut(s) 296
BspMAI CTGCAG 1 cut(s) 12
BspPI GGATC 2 cut(s) 60, 421
BsrFI RCCGGY 1 cut(s) 267
BssAI RCCGGY 1 cut(s) 267
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 3 cut(s) 35, 65, 413
BstC8I GCNNGC 2 cut(s) 269, 349
BstFNI CGCG 1 cut(s) 296
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 3 cut(s) 38, 68, 416
BstMBI GATC 3 cut(s) 35, 65, 413
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNSI RCATGY 1 cut(s) 424
BstSFI CTRYAG 1 cut(s) 8
BstUI CGCG 1 cut(s) 296
BstX2I RGATCY 1 cut(s) 65
BstXI CCANNNNNNTGG 1 cut(s) 26
BstYI RGATCY 1 cut(s) 65
BstZI CGGCCG 1 cut(s) 262
BsuRI GGCC 2 cut(s) 264, 452
BtsIMutI CAGTG 1 cut(s) 386
Cac8I GCNNGC 2 cut(s) 269, 349
CfoI GCGC 1 cut(s) 296
Cfr10I RCCGGY 1 cut(s) 267
Csp6I GTAC 1 cut(s) 149
CspCI CAANNNNNGTGG 4 cut(s) 121, 152, 156, 187
CviAII CATG 1 cut(s) 421
CviJI RGCY 3 cut(s) 196, 264, 452
CviKI_1 RGCY 3 cut(s) 196, 264, 452
CviQI GTAC 1 cut(s) 149
DpnI GATC 3 cut(s) 37, 67, 415
DpnII GATC 3 cut(s) 35, 65, 413
EaeI YGGCCR 1 cut(s) 262
EagI CGGCCG 1 cut(s) 262
EciI GGCGGA 1 cut(s) 302
EclXI CGGCCG 1 cut(s) 262
Eco52I CGGCCG 1 cut(s) 262
FaeI CATG 1 cut(s) 424
FaiI YATR 7 cut(s) 125, 188, 218, 235, 284, 402, 422
FaqI GGGAC 1 cut(s) 72
FatI CATG 1 cut(s) 420
FauI CCCGC 1 cut(s) 264
Fnu4HI GCNGC 2 cut(s) 8, 265
Fsp4HI GCNGC 2 cut(s) 8, 265
GlaI GCGC 1 cut(s) 295
GluI GCNGC 2 cut(s) 8, 265
GsuI CTGGAG 1 cut(s) 46
HaeIII GGCC 2 cut(s) 264, 452
HapII CCGG 1 cut(s) 268
HhaI GCGC 1 cut(s) 296
Hin1II CATG 1 cut(s) 424
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HinfI GANTC 4 cut(s) 15, 56, 167, 364
HpaII CCGG 1 cut(s) 268
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 1 cut(s) 19
Hpy188I TCNGA 1 cut(s) 33
Hpy188III TCNNGA 2 cut(s) 63, 164
Hpy8I GTNNAC 1 cut(s) 19
HpyCH4V TGCA 2 cut(s) 10, 441
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
Hsp92II CATG 1 cut(s) 424
HspAI GCGC 1 cut(s) 294
KroI GCCGGC 1 cut(s) 267
KroNI GCCGGC 1 cut(s) 269
Kzo9I GATC 3 cut(s) 35, 65, 413
LmnI GCTCC 1 cut(s) 216
LpnPI CCDG 4 cut(s) 76, 84, 281, 337
LweI GCATC 2 cut(s) 310, 450
MaeIII GTNAC 1 cut(s) 40
MalI GATC 3 cut(s) 37, 67, 415
MboI GATC 3 cut(s) 35, 65, 413
MboII GAAGA 1 cut(s) 329
MflI RGATCY 1 cut(s) 65
MluCI AATT 1 cut(s) 431
MlyI GAGTC 1 cut(s) 24
MmeI TCCRAC 1 cut(s) 11
MnlI CCTC 9 cut(s) 32, 40, 70, 92, 100, 168, 249, 291, 442
MreI CGCCGGCG 1 cut(s) 267
MroNI GCCGGC 1 cut(s) 267
MseI TTAA 3 cut(s) 120, 279, 460
MslI CAYNNNNRTG 1 cut(s) 24
MspI CCGG 1 cut(s) 268
MvnI CGCG 1 cut(s) 296
MwoI GCNNNNNNNGC 1 cut(s) 293
NaeI GCCGGC 1 cut(s) 269
NdeII GATC 3 cut(s) 35, 65, 413
NgoMIV GCCGGC 1 cut(s) 267
NlaIII CATG 1 cut(s) 424
NmuCI GTSAC 1 cut(s) 40
NspI RCATGY 1 cut(s) 424
OliI CACNNNNGTG 1 cut(s) 24
PdiI GCCGGC 1 cut(s) 269
PfeI GAWTC 3 cut(s) 56, 167, 364
PkrI GCNGC 2 cut(s) 9, 266
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
PshBI ATTAAT 1 cut(s) 279
PstI CTGCAG 1 cut(s) 12
PsuI RGATCY 1 cut(s) 65
RsaI GTAC 1 cut(s) 150
RsaNI GTAC 1 cut(s) 149
RseI CAYNNNNRTG 1 cut(s) 24
SaqAI TTAA 3 cut(s) 120, 279, 460
SatI GCNGC 2 cut(s) 8, 265
Sau3AI GATC 3 cut(s) 35, 65, 413
SchI GAGTC 1 cut(s) 24
SetI ASST 1 cut(s) 24
SfaNI GCATC 2 cut(s) 310, 450
SfcI CTRYAG 1 cut(s) 8
SgrAI CRCCGGYG 1 cut(s) 267
SmiMI CAYNNNNRTG 1 cut(s) 24
Sse9I AATT 1 cut(s) 431
SsiI CCGC 3 cut(s) 265, 271, 287
TasI AATT 1 cut(s) 431
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 3 cut(s) 56, 167, 364
Tru1I TTAA 3 cut(s) 120, 279, 460
Tru9I TTAA 3 cut(s) 120, 279, 460
TscAI CASTG 1 cut(s) 386
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 1 cut(s) 7
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 1 cut(s) 417
TspGWI ACGGA 3 cut(s) 59, 74, 166
TspRI CASTG 1 cut(s) 386
VspI ATTAAT 1 cut(s) 279
XceI RCATGY 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.