Rroxscaffold_6G00429660

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0019904
Physical Location & Seq
Forward (+)
49896477 .. 49898953
2477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00429660.1

Sequence Viewer

Length: 711 bp
ATGGGTCTGACATCTGAGAGAGACATGTCTAAGAACCGATTGCAAGAGGAGGCTCAGAAAAGTCGTTTAAAATTTCCCTCGTATTTACACACCCGGGAAGGACCGGATCATGCCCCAGAATTCAAAGCAACCGTCAAATTCAATGGAAAAATTTACAAAAGTCCTACCTTTTACCCTACTCTTAGACAGGCAGAGAATGCTGCAGCAGAAGAAGCATTGAACAAAGGTCAAAAAGGAGGGCATAAGGCCGCAGTACCGGTGAGTAGCAAAAACGGTAGTGCTTGCATGTTTAAGAACCGATTGCAAGAGGAGGCTCAGAAAAGTCGTTTAAATTTTCCCTCGTATTCATCCACTCGGGAAGGGCCGGATCATGCCCCACAATTCAAAGCAACCGTCAACTTCAATGGAAAAACTTACAATAGTCCTACCTTTTACCCTACTCTTAGACAGGCAGAGAATGCTGCAGCAGAAGTAGCATTGAACACAGTTGGAAAAGGAGGGCATAACCTAGCATTGGCCGCAGTACTGTACTGTGCTTCTAATGCAGGTTGTCCTATTCCTATTTCTACATGCATGGCTGCATCCCCAGTCCACAAGAGAACCCCGAAACGGGAGCAAACCGCCCTTCCGCCTTTCTCGAGTGCTGCAGGAGTAAACGTCTTTGGAGCATCTCAAGAGGAAAATCATCGATTTTGTTGTTCAGTGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box dsrm

Protein Analysis

236

Amino Acids

25.65

Weight (kDa)

9.45

Isoelectric Point (pI)

57.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 11 - 75 4e-14 Double-stranded RNA binding motif
dsrm PF00035 98 - 161 9.3e-13 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019904)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0452621
rosa_multiflora Rmu_sc0011540.1_g000001
rosa_roxburghii Rroxscaffold_6G00429660
rosa_rugosa Rorug02G0641800
rosa_samantha Rh3AG044300 Rh3BG046600
rosa_wichuraiana Rw3G003450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 536
AciI CCGC 4 cut(s) 249, 519, 621, 629
AclWI GGATC 2 cut(s) 114, 375
AcoI YGGCCR 1 cut(s) 516
AcsI RAATTY 5 cut(s) 71, 119, 137, 150, 331
AfaI GTAC 3 cut(s) 255, 525, 530
AfiI CCNNNNNNNGG 3 cut(s) 514, 609, 610
AflIII ACRYGT 1 cut(s) 24
AgeI ACCGGT 1 cut(s) 256
AgsI TTSAA 6 cut(s) 124, 142, 220, 385, 403, 481
Alw26I GTCTC 1 cut(s) 15
AlwI GGATC 2 cut(s) 114, 375
Ama87I CYCGRG 3 cut(s) 93, 354, 637
AoxI GGCC 3 cut(s) 246, 362, 516
ApeKI GCWGC 6 cut(s) 200, 203, 461, 464, 578, 644
ApoI RAATTY 5 cut(s) 71, 119, 137, 150, 331
ArsI GACNNNNNNTTYG 4 cut(s) 117, 149, 378, 410
AsiGI ACCGGT 1 cut(s) 256
AspS9I GGNCC 2 cut(s) 101, 362
AsuC2I CCSGG 2 cut(s) 94, 95
AsuHPI GGTGA 1 cut(s) 271
AvaI CYCGRG 3 cut(s) 93, 354, 637
AvaII GGWCC 1 cut(s) 101
BbvI GCAGC 6 cut(s) 187, 215, 448, 476, 565, 631
BcnI CCSGG 2 cut(s) 94, 95
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 1 cut(s) 509
BfmI CTRYAG 3 cut(s) 201, 462, 645
BfuAI ACCTGC 1 cut(s) 536
BisI GCNGC 8 cut(s) 201, 204, 249, 462, 465, 519, 579, 645
BlsI GCNGC 8 cut(s) 202, 205, 250, 463, 466, 520, 580, 646
BmcAI AGTACT 1 cut(s) 525
Bme1390I CCNGG 2 cut(s) 94, 95
Bme18I GGWCC 1 cut(s) 101
BmeT110I CYCGRG 3 cut(s) 93, 354, 637
BmgT120I GGNCC 2 cut(s) 101, 362
BmrFI CCNGG 2 cut(s) 94, 95
BmrI ACTGGG 1 cut(s) 581
BmsI GCATC 2 cut(s) 590, 677
BmuI ACTGGG 1 cut(s) 581
BpuEI CTTGAG 1 cut(s) 657
BpuMI CCSGG 2 cut(s) 94, 95
Bsa29I ATCGAT 1 cut(s) 688
BsaJI CCNNGG 1 cut(s) 93
BsaWI WCCGGW 2 cut(s) 103, 256
BsaXI ACNNNNNCTCC 2 cut(s) 642, 672
Bsc4I CCNNNNNNNGG 3 cut(s) 514, 609, 610
Bse118I RCCGGY 1 cut(s) 256
Bse1I ACTGG 1 cut(s) 587
BseCI ATCGAT 1 cut(s) 688
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 2 cut(s) 347, 581
BseLI CCNNNNNNNGG 3 cut(s) 514, 609, 610
BseMII CTCAG 3 cut(s) 6, 68, 329
BseNI ACTGG 1 cut(s) 587
BseRI GAGGAG 2 cut(s) 62, 323
BseXI GCAGC 6 cut(s) 187, 215, 448, 476, 565, 631
BshFI GGCC 3 cut(s) 248, 364, 518
BshTI ACCGGT 1 cut(s) 256
BshVI ATCGAT 1 cut(s) 688
BsiHKCI CYCGRG 3 cut(s) 93, 354, 637
BsiSI CCGG 4 cut(s) 94, 104, 257, 365
BslI CCNNNNNNNGG 3 cut(s) 514, 609, 610
BsmAI GTCTC 1 cut(s) 15
BsmI GAATGC 2 cut(s) 202, 463
BsnI GGCC 3 cut(s) 248, 364, 518
BsoBI CYCGRG 3 cut(s) 93, 354, 637
Bsp143I GATC 2 cut(s) 106, 367
BspACI CCGC 4 cut(s) 249, 519, 621, 629
BspANI GGCC 3 cut(s) 248, 364, 518
BspCNI CTCAG 3 cut(s) 7, 67, 328
BspDI ATCGAT 1 cut(s) 688
BspMAI CTGCAG 3 cut(s) 205, 466, 649
BspMI ACCTGC 1 cut(s) 536
BspPI GGATC 2 cut(s) 114, 375
BsrFI RCCGGY 1 cut(s) 256
BsrI ACTGG 1 cut(s) 587
BssAI RCCGGY 1 cut(s) 256
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 2 cut(s) 106, 367
Bst4CI ACNGT 6 cut(s) 133, 275, 394, 487, 528, 533
BstAPI GCANNNNNTGC 2 cut(s) 197, 458
BstC8I GCNNGC 1 cut(s) 283
BstDEI CTNAG 6 cut(s) 15, 30, 54, 182, 315, 443
BstF5I GGATG 2 cut(s) 347, 581
BstKTI GATC 2 cut(s) 109, 370
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 2 cut(s) 106, 367
BstMWI GCNNNNNNNGC 6 cut(s) 197, 212, 458, 473, 518, 542
BstNSI RCATGY 3 cut(s) 28, 289, 573
BstSCI CCNGG 2 cut(s) 92, 93
BstSFI CTRYAG 3 cut(s) 201, 462, 645
BstV1I GCAGC 6 cut(s) 187, 215, 448, 476, 565, 631
Bsu15I ATCGAT 1 cut(s) 688
BsuRI GGCC 3 cut(s) 248, 364, 518
BsuTUI ATCGAT 1 cut(s) 688
BtsCI GGATG 2 cut(s) 347, 581
BtsIMutI CAGTG 1 cut(s) 708
BveI ACCTGC 1 cut(s) 536
Cac8I GCNNGC 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 256
Cfr13I GGNCC 2 cut(s) 101, 362
Cfr9I CCCGGG 1 cut(s) 93
ClaI ATCGAT 1 cut(s) 688
Csp6I GTAC 3 cut(s) 254, 524, 529
CspAI ACCGGT 1 cut(s) 256
CviAII CATG 6 cut(s) 25, 110, 286, 371, 570, 574
CviJI RGCY 6 cut(s) 53, 248, 314, 364, 518, 578
CviKI_1 RGCY 6 cut(s) 53, 248, 314, 364, 518, 578
CviQI GTAC 3 cut(s) 254, 524, 529
DdeI CTNAG 6 cut(s) 15, 30, 54, 182, 315, 443
DpnI GATC 2 cut(s) 108, 369
DpnII GATC 2 cut(s) 106, 367
DraI TTTAAA 2 cut(s) 69, 330
EaeI YGGCCR 1 cut(s) 516
EciI GGCGGA 1 cut(s) 618
Eco47I GGWCC 1 cut(s) 101
Eco88I CYCGRG 3 cut(s) 93, 354, 637
EcoRI GAATTC 1 cut(s) 119
EcoT22I ATGCAT 1 cut(s) 575
FaeI CATG 6 cut(s) 28, 113, 289, 374, 573, 577
FaiI YATR 9 cut(s) 26, 111, 243, 287, 372, 504, 571, 575, 709
FatI CATG 6 cut(s) 24, 109, 285, 370, 569, 573
Fnu4HI GCNGC 8 cut(s) 201, 204, 249, 462, 465, 519, 579, 645
FokI GGATG 2 cut(s) 334, 568
Fsp4HI GCNGC 8 cut(s) 201, 204, 249, 462, 465, 519, 579, 645
FspBI CTAG 1 cut(s) 509
GluI GCNGC 8 cut(s) 201, 204, 249, 462, 465, 519, 579, 645
HaeIII GGCC 3 cut(s) 248, 364, 518
HapII CCGG 4 cut(s) 94, 104, 257, 365
Hin1II CATG 6 cut(s) 28, 113, 289, 374, 573, 577
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HpaII CCGG 4 cut(s) 94, 104, 257, 365
HphI GGTGA 1 cut(s) 271
Hpy166II GTNNAC 3 cut(s) 397, 592, 655
Hpy188I TCNGA 4 cut(s) 9, 16, 57, 318
Hpy188III TCNNGA 3 cut(s) 356, 637, 674
Hpy8I GTNNAC 3 cut(s) 397, 592, 655
HpyAV CCTTC 3 cut(s) 92, 353, 635
HpyCH4III ACNGT 6 cut(s) 133, 275, 394, 487, 528, 533
HpyCH4IV ACGT 1 cut(s) 657
HpyCH4V TGCA 9 cut(s) 43, 203, 285, 304, 464, 545, 573, 581, 647
HpyF10VI GCNNNNNNNGC 6 cut(s) 197, 212, 458, 473, 518, 542
HpyF3I CTNAG 6 cut(s) 15, 30, 54, 182, 315, 443
HpySE526I ACGT 1 cut(s) 657
Hsp92II CATG 6 cut(s) 28, 113, 289, 374, 573, 577
Kzo9I GATC 2 cut(s) 106, 367
LmnI GCTCC 2 cut(s) 613, 665
Lsp1109I GCAGC 6 cut(s) 187, 215, 448, 476, 565, 631
LweI GCATC 2 cut(s) 590, 677
MaeI CTAG 1 cut(s) 509
MaeII ACGT 1 cut(s) 657
MalI GATC 2 cut(s) 108, 369
MboI GATC 2 cut(s) 106, 367
MboII GAAGA 1 cut(s) 221
MluCI AATT 6 cut(s) 71, 119, 137, 150, 331, 380
MmeI TCCRAC 1 cut(s) 469
MnlI CCTC 9 cut(s) 40, 43, 88, 230, 301, 304, 349, 491, 670
Mph1103I ATGCAT 1 cut(s) 575
MseI TTAA 3 cut(s) 68, 291, 329
MspI CCGG 4 cut(s) 94, 104, 257, 365
MspR9I CCNGG 2 cut(s) 94, 95
Mva1269I GAATGC 2 cut(s) 202, 463
MwoI GCNNNNNNNGC 6 cut(s) 197, 212, 458, 473, 518, 542
NciI CCSGG 2 cut(s) 94, 95
NdeII GATC 2 cut(s) 106, 367
NlaIII CATG 6 cut(s) 28, 113, 289, 374, 573, 577
NsiI ATGCAT 1 cut(s) 575
NspI RCATGY 3 cut(s) 28, 289, 573
PaeR7I CTCGAG 1 cut(s) 637
PciI ACATGT 1 cut(s) 24
PctI GAATGC 2 cut(s) 202, 463
PinAI ACCGGT 1 cut(s) 256
PkrI GCNGC 8 cut(s) 202, 205, 250, 463, 466, 520, 580, 646
PscI ACATGT 1 cut(s) 24
PspPI GGNCC 2 cut(s) 101, 362
PstI CTGCAG 3 cut(s) 205, 466, 649
RsaI GTAC 3 cut(s) 255, 525, 530
RsaNI GTAC 3 cut(s) 254, 524, 529
SaqAI TTAA 3 cut(s) 68, 291, 329
SatI GCNGC 8 cut(s) 201, 204, 249, 462, 465, 519, 579, 645
Sau3AI GATC 2 cut(s) 106, 367
Sau96I GGNCC 2 cut(s) 101, 362
ScaI AGTACT 1 cut(s) 525
ScrFI CCNGG 2 cut(s) 94, 95
SetI ASST 6 cut(s) 170, 229, 431, 510, 550, 660
SfaNI GCATC 2 cut(s) 590, 677
SfcI CTRYAG 3 cut(s) 201, 462, 645
Sfr274I CTCGAG 1 cut(s) 637
SinI GGWCC 1 cut(s) 101
SlaI CTCGAG 1 cut(s) 637
SmaI CCCGGG 1 cut(s) 95
SmlI CTYRAG 2 cut(s) 637, 672
SmoI CTYRAG 2 cut(s) 637, 672
Sse9I AATT 6 cut(s) 71, 119, 137, 150, 331, 380
SsiI CCGC 4 cut(s) 249, 519, 621, 629
SspMI CTAG 1 cut(s) 509
StyD4I CCNGG 2 cut(s) 92, 93
TaaI ACNGT 6 cut(s) 133, 275, 394, 487, 528, 533
TaiI ACGT 1 cut(s) 660
TaqI TCGA 2 cut(s) 638, 688
TasI AATT 6 cut(s) 71, 119, 137, 150, 331, 380
TatI WGTACW 2 cut(s) 523, 528
TauI GCSGC 2 cut(s) 251, 521
Tru1I TTAA 3 cut(s) 68, 291, 329
Tru9I TTAA 3 cut(s) 68, 291, 329
TscAI CASTG 1 cut(s) 708
TseI GCWGC 6 cut(s) 200, 203, 461, 464, 578, 644
TspDTI ATGAA 1 cut(s) 336
TspMI CCCGGG 1 cut(s) 93
TspRI CASTG 1 cut(s) 708
VpaK11BI GGWCC 1 cut(s) 101
XapI RAATTY 5 cut(s) 71, 119, 137, 150, 331
XceI RCATGY 3 cut(s) 28, 289, 573
XhoI CTCGAG 1 cut(s) 637
XmaI CCCGGG 1 cut(s) 93
XspI CTAG 1 cut(s) 509
ZrmI AGTACT 1 cut(s) 525
Zsp2I ATGCAT 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.