Rh3BG046600

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0019904
Physical Location & Seq
Forward (+)
3004361 .. 3006154
1794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG046600.1

Sequence Viewer

Length: 327 bp
ATGGGTCTGACATCTGAGAGAGACATATATAAGAACCGATTGCAAGAGGAGGCTCAGAAAAGACGTTTAAATTTTCCCTCGTATTCATCCACCCGGGAAAGACCGGATCATGCCCCACGATTCAAAGCAACCGTCAACTTCGATGGAAAAACTTTTGAAAGTCCTGACTCTTACCCTACTCTTCGAGAGGCAGAGAATGCTGCAGCAGAAGTAGCATTGAACACAGTTGAAAAAAGAGGGCATAACCTAGCATTGGCCGCAGTACTGGCTGTCCTATTCATATTTCTATATGCATGGCTGTATCCCGAGTCCACAAGAGAACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

108

Amino Acids

12.39

Weight (kDa)

6.74

Isoelectric Point (pI)

55.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 10 - 74 2.4e-14 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019904)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0452621
rosa_multiflora Rmu_sc0011540.1_g000001
rosa_roxburghii Rroxscaffold_6G00429660
rosa_rugosa Rorug02G0641800
rosa_samantha Rh3AG044300 Rh3BG046600
rosa_wichuraiana Rw3G003450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 258
AclWI GGATC 1 cut(s) 114
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 1 cut(s) 70
AfaI GTAC 1 cut(s) 264
AfiI CCNNNNNNNGG 1 cut(s) 253
AgsI TTSAA 4 cut(s) 124, 158, 220, 230
Alw26I GTCTC 1 cut(s) 15
AlwI GGATC 1 cut(s) 114
Ama87I CYCGRG 2 cut(s) 93, 305
AoxI GGCC 1 cut(s) 255
ApeKI GCWGC 2 cut(s) 200, 203
ApoI RAATTY 1 cut(s) 70
ArsI GACNNNNNNTTYG 2 cut(s) 117, 149
AsuC2I CCSGG 2 cut(s) 94, 95
AvaI CYCGRG 2 cut(s) 93, 305
BbvI GCAGC 2 cut(s) 187, 215
BccI CCATC 1 cut(s) 137
BciVI GTATCC 1 cut(s) 312
BcnI CCSGG 2 cut(s) 94, 95
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 1 cut(s) 248
BfmI CTRYAG 1 cut(s) 201
BfuI GTATCC 1 cut(s) 312
BisI GCNGC 3 cut(s) 201, 204, 258
BlsI GCNGC 3 cut(s) 202, 205, 259
BmcAI AGTACT 1 cut(s) 264
Bme1390I CCNGG 2 cut(s) 94, 95
BmeT110I CYCGRG 2 cut(s) 93, 305
BmrFI CCNGG 2 cut(s) 94, 95
BpuMI CCSGG 2 cut(s) 94, 95
BsaJI CCNNGG 1 cut(s) 93
BsaWI WCCGGW 1 cut(s) 103
Bsc4I CCNNNNNNNGG 1 cut(s) 253
Bse1I ACTGG 1 cut(s) 270
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 1 cut(s) 86
BseLI CCNNNNNNNGG 1 cut(s) 253
BseMII CTCAG 2 cut(s) 6, 68
BseNI ACTGG 1 cut(s) 270
BseRI GAGGAG 1 cut(s) 62
BseXI GCAGC 2 cut(s) 187, 215
BshFI GGCC 1 cut(s) 257
BsiHKCI CYCGRG 2 cut(s) 93, 305
BsiSI CCGG 2 cut(s) 94, 104
BslI CCNNNNNNNGG 1 cut(s) 253
BsmAI GTCTC 1 cut(s) 15
BsmI GAATGC 1 cut(s) 202
BsnI GGCC 1 cut(s) 257
BsoBI CYCGRG 2 cut(s) 93, 305
Bsp143I GATC 1 cut(s) 106
BspACI CCGC 1 cut(s) 258
BspANI GGCC 1 cut(s) 257
BspCNI CTCAG 2 cut(s) 7, 67
BspMAI CTGCAG 1 cut(s) 205
BspPI GGATC 1 cut(s) 114
BsrI ACTGG 1 cut(s) 270
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 1 cut(s) 106
Bst4CI ACNGT 2 cut(s) 133, 226
Bst6I CTCTTC 1 cut(s) 186
BstAPI GCANNNNNTGC 1 cut(s) 197
BstDEI CTNAG 2 cut(s) 15, 54
BstF5I GGATG 1 cut(s) 86
BstKTI GATC 1 cut(s) 109
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 1 cut(s) 106
BstMWI GCNNNNNNNGC 4 cut(s) 197, 212, 257, 266
BstSCI CCNGG 2 cut(s) 92, 93
BstSFI CTRYAG 1 cut(s) 201
BstV1I GCAGC 2 cut(s) 187, 215
BsuI GTATCC 1 cut(s) 312
BsuRI GGCC 1 cut(s) 257
BtsCI GGATG 1 cut(s) 86
Cfr9I CCCGGG 1 cut(s) 93
Csp6I GTAC 1 cut(s) 263
CviAII CATG 2 cut(s) 110, 294
CviJI RGCY 4 cut(s) 53, 257, 269, 298
CviKI_1 RGCY 4 cut(s) 53, 257, 269, 298
CviQI GTAC 1 cut(s) 263
DdeI CTNAG 2 cut(s) 15, 54
DpnI GATC 1 cut(s) 108
DpnII GATC 1 cut(s) 106
DraI TTTAAA 1 cut(s) 69
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Eco88I CYCGRG 2 cut(s) 93, 305
EcoT22I ATGCAT 1 cut(s) 295
FaeI CATG 2 cut(s) 113, 297
FaiI YATR 9 cut(s) 26, 28, 30, 111, 243, 281, 289, 291, 295
FatI CATG 2 cut(s) 109, 293
Fnu4HI GCNGC 3 cut(s) 201, 204, 258
FokI GGATG 1 cut(s) 73
Fsp4HI GCNGC 3 cut(s) 201, 204, 258
FspBI CTAG 1 cut(s) 248
GluI GCNGC 3 cut(s) 201, 204, 258
HaeIII GGCC 1 cut(s) 257
HapII CCGG 2 cut(s) 94, 104
Hin1II CATG 2 cut(s) 113, 297
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 3 cut(s) 120, 167, 308
HpaII CCGG 2 cut(s) 94, 104
Hpy166II GTNNAC 2 cut(s) 136, 312
Hpy188I TCNGA 3 cut(s) 9, 16, 57
Hpy188III TCNNGA 3 cut(s) 164, 185, 305
Hpy8I GTNNAC 2 cut(s) 136, 312
HpyCH4III ACNGT 2 cut(s) 133, 226
HpyCH4IV ACGT 1 cut(s) 64
HpyCH4V TGCA 3 cut(s) 43, 203, 293
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 212, 257, 266
HpyF3I CTNAG 2 cut(s) 15, 54
HpySE526I ACGT 1 cut(s) 64
Hsp92II CATG 2 cut(s) 113, 297
Kzo9I GATC 1 cut(s) 106
LpnPI CCDG 4 cut(s) 107, 117, 177, 251
Lsp1109I GCAGC 2 cut(s) 187, 215
MaeI CTAG 1 cut(s) 248
MaeII ACGT 1 cut(s) 64
MalI GATC 1 cut(s) 108
MboI GATC 1 cut(s) 106
MboII GAAGA 1 cut(s) 173
MluCI AATT 1 cut(s) 70
MlyI GAGTC 2 cut(s) 161, 317
MnlI CCTC 5 cut(s) 40, 43, 88, 181, 230
Mph1103I ATGCAT 1 cut(s) 295
MseI TTAA 1 cut(s) 68
MspI CCGG 2 cut(s) 94, 104
MspR9I CCNGG 2 cut(s) 94, 95
Mva1269I GAATGC 1 cut(s) 202
MwoI GCNNNNNNNGC 4 cut(s) 197, 212, 257, 266
NciI CCSGG 2 cut(s) 94, 95
NdeII GATC 1 cut(s) 106
NlaIII CATG 2 cut(s) 113, 297
NsiI ATGCAT 1 cut(s) 295
PctI GAATGC 1 cut(s) 202
PfeI GAWTC 1 cut(s) 120
PkrI GCNGC 3 cut(s) 202, 205, 259
PleI GAGTC 2 cut(s) 161, 316
PpsI GAGTC 2 cut(s) 161, 316
PstI CTGCAG 1 cut(s) 205
RsaI GTAC 1 cut(s) 264
RsaNI GTAC 1 cut(s) 263
SaqAI TTAA 1 cut(s) 68
SatI GCNGC 3 cut(s) 201, 204, 258
Sau3AI GATC 1 cut(s) 106
ScaI AGTACT 1 cut(s) 264
SchI GAGTC 2 cut(s) 161, 317
ScrFI CCNGG 2 cut(s) 94, 95
SetI ASST 2 cut(s) 67, 249
SfcI CTRYAG 1 cut(s) 201
SmaI CCCGGG 1 cut(s) 95
Sse9I AATT 1 cut(s) 70
SsiI CCGC 1 cut(s) 258
SspMI CTAG 1 cut(s) 248
StyD4I CCNGG 2 cut(s) 92, 93
TaaI ACNGT 2 cut(s) 133, 226
TaiI ACGT 1 cut(s) 67
TaqI TCGA 2 cut(s) 141, 184
TasI AATT 1 cut(s) 70
TatI WGTACW 1 cut(s) 262
TauI GCSGC 1 cut(s) 260
TfiI GAWTC 1 cut(s) 120
Tru1I TTAA 1 cut(s) 68
Tru9I TTAA 1 cut(s) 68
TseI GCWGC 2 cut(s) 200, 203
TspDTI ATGAA 2 cut(s) 75, 268
TspMI CCCGGG 1 cut(s) 93
XapI RAATTY 1 cut(s) 70
XmaI CCCGGG 1 cut(s) 93
XspI CTAG 1 cut(s) 248
ZrmI AGTACT 1 cut(s) 264
Zsp2I ATGCAT 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.