Rroxscaffold_7G00185260

Zinc-binding dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0020395
Physical Location & Seq
Reverse (-)
24676740 .. 24678576
1837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00185260.1

Sequence Viewer

Length: 576 bp
ATGGCGGTAAAGCTTATGCATGCGGTTCAGTACAACAGTTATGGTGGAGGTCCCTCTGGTTTAAAGCATGTTGAGATTCCAATCCCAACTCCGAAGAAAGATGAGGTTTTGCTGAAATTAGAAGCAGCTAGTCTGAATCCCGTTGATTGGACAATTCAGACAGGCTTGATGCGGCCTATATTTTTCCCGCGCCACTTTCCTCATATTCCTGGGGCAGATCTGAATAGCCCTTCTGGTCGGAAATATGATGCTGTGATCCACTGTACAACGGGCATTCCTTGGTCCACTTTTAATCCGAATTTGAGTCCAAATGGGAAGGTCATAGACATTACTCCCGGTCCAAAGGCTATGCTTACTTTCACTCTACAGAAACTCACCTGCTCCAAGAAGCAGCTGGTGCCGCTGCTGCCGGTTATTCGCAAGGCTGAGAACCTGGATTATCTTGTCAAGTTGGTGAAGGAAGGAAAGCTCAAGACGGTGATAGACTCGACGCATCCTCTGAGCAAGGCTGAAGATGCTTGGGGTAAGAGTATCGACGGCCATGCTACTGGGAAGATCATTGTGGAGCCTAAATAG

Protein Analysis

191

Amino Acids

20.93

Weight (kDa)

9.47

Isoelectric Point (pI)

27.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N_2 PF13602 77 - 188 3.8e-16 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020395)

Species Orthologous Gene IDs
malus_domestica MD05G1168100.v1.1 MD05G1168500.v1.1
pyrus_communis pycom05g15590 pycom05g15640
rosa_multiflora Rmu_sc0001521.1_g000001
rosa_roxburghii Rroxscaffold_7G00185260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 386
Acc36I ACCTGC 1 cut(s) 386
AccB1I GGYRCC 1 cut(s) 397
AccII CGCG 1 cut(s) 190
AciI CCGC 5 cut(s) 5, 23, 172, 188, 401
AclWI GGATC 1 cut(s) 250
AcoI YGGCCR 1 cut(s) 538
AcsI RAATTY 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 531
AfaI GTAC 2 cut(s) 32, 265
AfiI CCNNNNNNNGG 1 cut(s) 147
AjnI CCWGG 2 cut(s) 208, 432
AluBI AGCT 4 cut(s) 13, 128, 394, 469
AluI AGCT 4 cut(s) 13, 128, 394, 469
AlwI GGATC 1 cut(s) 250
AoxI GGCC 2 cut(s) 173, 538
ApeKI GCWGC 4 cut(s) 125, 391, 403, 406
ApoI RAATTY 1 cut(s) 298
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 3 cut(s) 50, 282, 338
AsuC2I CCSGG 1 cut(s) 336
AsuHPI GGTGA 3 cut(s) 367, 466, 490
AvaII GGWCC 3 cut(s) 50, 282, 338
BanI GGYRCC 1 cut(s) 397
BbvI GCAGC 4 cut(s) 137, 390, 393, 403
BceAI ACGGC 1 cut(s) 553
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BciT130I CCWGG 2 cut(s) 210, 434
BcnI CCSGG 1 cut(s) 336
BfaI CTAG 1 cut(s) 129
BfmI CTRYAG 1 cut(s) 365
BfuAI ACCTGC 1 cut(s) 386
BglII AGATCT 1 cut(s) 217
BisI GCNGC 6 cut(s) 126, 173, 392, 401, 404, 407
BlsI GCNGC 6 cut(s) 127, 174, 393, 402, 405, 408
Bme1390I CCNGG 3 cut(s) 210, 336, 434
Bme18I GGWCC 3 cut(s) 50, 282, 338
BmgT120I GGNCC 3 cut(s) 50, 282, 338
BmiI GGNNCC 3 cut(s) 52, 399, 567
BmrFI CCNGG 3 cut(s) 210, 336, 434
BmrI ACTGGG 1 cut(s) 558
BmsI GCATC 4 cut(s) 159, 238, 502, 505
BmuI ACTGGG 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 455
BpuMI CCSGG 1 cut(s) 336
BsaBI GATNNNNATC 1 cut(s) 80
BsaJI CCNNGG 2 cut(s) 209, 278
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse118I RCCGGY 1 cut(s) 409
Bse1I ACTGG 1 cut(s) 553
Bse8I GATNNNNATC 1 cut(s) 80
BseBI CCWGG 2 cut(s) 210, 434
BseDI CCNNGG 2 cut(s) 209, 278
BseGI GGATG 1 cut(s) 493
BseJI GATNNNNATC 1 cut(s) 80
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMII CTCAG 2 cut(s) 417, 491
BseNI ACTGG 1 cut(s) 553
BseXI GCAGC 4 cut(s) 137, 390, 393, 403
Bsh1236I CGCG 1 cut(s) 190
BshFI GGCC 2 cut(s) 175, 540
BshNI GGYRCC 1 cut(s) 397
BsiSI CCGG 2 cut(s) 336, 410
BslFI GGGAC 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 36
BsmI GAATGC 1 cut(s) 273
BsnI GGCC 2 cut(s) 175, 540
Bsp1407I TGTACA 1 cut(s) 263
Bsp143I GATC 3 cut(s) 217, 255, 555
BspACI CCGC 5 cut(s) 5, 23, 172, 188, 401
BspANI GGCC 2 cut(s) 175, 540
BspCNI CTCAG 2 cut(s) 418, 492
BspFNI CGCG 1 cut(s) 190
BspLI GGNNCC 3 cut(s) 52, 399, 567
BspMI ACCTGC 1 cut(s) 386
BspPI GGATC 1 cut(s) 250
BspT107I GGYRCC 1 cut(s) 397
BsrFI RCCGGY 1 cut(s) 409
BsrGI TGTACA 1 cut(s) 263
BsrI ACTGG 1 cut(s) 553
BssAI RCCGGY 1 cut(s) 409
BssECI CCNNGG 2 cut(s) 209, 278
BssMI GATC 3 cut(s) 217, 255, 555
BssT1I CCWWGG 1 cut(s) 278
Bst2UI CCWGG 2 cut(s) 210, 434
Bst4CI ACNGT 3 cut(s) 38, 263, 478
BstAPI GCANNNNNTGC 1 cut(s) 397
BstAUI TGTACA 1 cut(s) 263
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 2 cut(s) 426, 500
BstF5I GGATG 1 cut(s) 493
BstFNI CGCG 1 cut(s) 190
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 3 cut(s) 220, 258, 558
BstMBI GATC 3 cut(s) 217, 255, 555
BstMWI GCNNNNNNNGC 4 cut(s) 397, 400, 406, 515
BstNI CCWGG 2 cut(s) 210, 434
BstNSI RCATGY 2 cut(s) 23, 71
BstSCI CCNGG 3 cut(s) 208, 334, 432
BstSFI CTRYAG 1 cut(s) 365
BstUI CGCG 1 cut(s) 190
BstV1I GCAGC 4 cut(s) 137, 390, 393, 403
BstX2I RGATCY 1 cut(s) 217
BstXI CCANNNNNNTGG 1 cut(s) 548
BstYI RGATCY 1 cut(s) 217
BsuRI GGCC 2 cut(s) 175, 540
BtsCI GGATG 1 cut(s) 493
BtsIMutI CAGTG 1 cut(s) 259
BveI ACCTGC 1 cut(s) 386
Cac8I GCNNGC 1 cut(s) 21
CfoI GCGC 1 cut(s) 192
Cfr10I RCCGGY 1 cut(s) 409
Cfr13I GGNCC 3 cut(s) 50, 282, 338
CseI GACGC 1 cut(s) 499
Csp6I GTAC 2 cut(s) 31, 264
CviAII CATG 3 cut(s) 20, 68, 542
CviQI GTAC 2 cut(s) 31, 264
DdeI CTNAG 2 cut(s) 426, 500
DpnI GATC 3 cut(s) 219, 257, 557
DpnII GATC 3 cut(s) 217, 255, 555
DraI TTTAAA 1 cut(s) 63
EaeI YGGCCR 1 cut(s) 538
Eco130I CCWWGG 1 cut(s) 278
Eco47I GGWCC 3 cut(s) 50, 282, 338
Eco57I CTGAAG 1 cut(s) 531
EcoO109I RGGNCCY 1 cut(s) 50
EcoRII CCWGG 2 cut(s) 208, 432
EcoT14I CCWWGG 1 cut(s) 278
EcoT22I ATGCAT 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 278
FaeI CATG 3 cut(s) 23, 71, 545
FaqI GGGAC 1 cut(s) 36
FatI CATG 3 cut(s) 19, 67, 541
FauI CCCGC 1 cut(s) 195
Fnu4HI GCNGC 6 cut(s) 126, 173, 392, 401, 404, 407
FokI GGATG 1 cut(s) 480
Fsp4HI GCNGC 6 cut(s) 126, 173, 392, 401, 404, 407
FspBI CTAG 1 cut(s) 129
GlaI GCGC 1 cut(s) 191
GluI GCNGC 6 cut(s) 126, 173, 392, 401, 404, 407
HaeIII GGCC 2 cut(s) 175, 540
HapII CCGG 2 cut(s) 336, 410
HgaI GACGC 1 cut(s) 499
HhaI GCGC 1 cut(s) 192
Hin1II CATG 3 cut(s) 23, 71, 545
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 4 cut(s) 76, 136, 304, 485
HpaII CCGG 2 cut(s) 336, 410
HphI GGTGA 3 cut(s) 367, 466, 490
Hpy166II GTNNAC 1 cut(s) 285
Hpy188I TCNGA 7 cut(s) 93, 135, 159, 222, 240, 297, 501
Hpy188III TCNNGA 1 cut(s) 472
Hpy8I GTNNAC 1 cut(s) 285
Hpy99I CGWCG 2 cut(s) 493, 539
HpyAV CCTTC 4 cut(s) 240, 310, 451, 455
HpyCH4III ACNGT 3 cut(s) 38, 263, 478
HpyCH4V TGCA 1 cut(s) 19
HpyF10VI GCNNNNNNNGC 4 cut(s) 397, 400, 406, 515
HpyF3I CTNAG 2 cut(s) 426, 500
Hsp92II CATG 3 cut(s) 23, 71, 545
HspAI GCGC 1 cut(s) 190
Kzo9I GATC 3 cut(s) 217, 255, 555
LmnI GCTCC 2 cut(s) 386, 565
Lsp1109I GCAGC 4 cut(s) 137, 390, 393, 403
LweI GCATC 4 cut(s) 159, 238, 502, 505
MaeI CTAG 1 cut(s) 129
MalI GATC 3 cut(s) 219, 257, 557
MboI GATC 3 cut(s) 217, 255, 555
MboII GAAGA 3 cut(s) 106, 524, 565
MflI RGATCY 1 cut(s) 217
MluCI AATT 3 cut(s) 116, 153, 298
MlyI GAGTC 2 cut(s) 313, 479
MmeI TCCRAC 1 cut(s) 218
MnlI CCTC 5 cut(s) 41, 64, 97, 210, 507
Mph1103I ATGCAT 1 cut(s) 21
MseI TTAA 2 cut(s) 62, 291
MspA1I CMGCKG 2 cut(s) 394, 403
MspI CCGG 2 cut(s) 336, 410
MspR9I CCNGG 3 cut(s) 210, 336, 434
Mva1269I GAATGC 1 cut(s) 273
MvaI CCWGG 2 cut(s) 210, 434
MvnI CGCG 1 cut(s) 190
MwoI GCNNNNNNNGC 4 cut(s) 397, 400, 406, 515
NciI CCSGG 1 cut(s) 336
NdeII GATC 3 cut(s) 217, 255, 555
NlaIII CATG 3 cut(s) 23, 71, 545
NlaIV GGNNCC 3 cut(s) 52, 399, 567
NsiI ATGCAT 1 cut(s) 21
NspI RCATGY 2 cut(s) 23, 71
PaeI GCATGC 1 cut(s) 23
PaqCI CACCTGC 1 cut(s) 386
PctI GAATGC 1 cut(s) 273
PfeI GAWTC 2 cut(s) 76, 136
PkrI GCNGC 6 cut(s) 127, 174, 393, 402, 405, 408
PleI GAGTC 2 cut(s) 312, 479
PpsI GAGTC 2 cut(s) 312, 479
PpuMI RGGWCCY 1 cut(s) 50
Psp5II RGGWCCY 1 cut(s) 50
Psp6I CCWGG 2 cut(s) 208, 432
PspGI CCWGG 2 cut(s) 208, 432
PspN4I GGNNCC 3 cut(s) 52, 399, 567
PspPI GGNCC 3 cut(s) 50, 282, 338
PspPPI RGGWCCY 1 cut(s) 50
PsuI RGATCY 1 cut(s) 217
PvuII CAGCTG 1 cut(s) 394
RsaI GTAC 2 cut(s) 32, 265
RsaNI GTAC 2 cut(s) 31, 264
SaqAI TTAA 2 cut(s) 62, 291
SatI GCNGC 6 cut(s) 126, 173, 392, 401, 404, 407
Sau3AI GATC 3 cut(s) 217, 255, 555
Sau96I GGNCC 3 cut(s) 50, 282, 338
SchI GAGTC 2 cut(s) 313, 479
ScrFI CCNGG 3 cut(s) 210, 336, 434
SetI ASST 9 cut(s) 15, 52, 108, 130, 321, 380, 396, 435, 471
SfaNI GCATC 4 cut(s) 159, 238, 502, 505
SfcI CTRYAG 1 cut(s) 365
SinI GGWCC 3 cut(s) 50, 282, 338
SmlI CTYRAG 1 cut(s) 470
SmoI CTYRAG 1 cut(s) 470
SphI GCATGC 1 cut(s) 23
Sse9I AATT 3 cut(s) 116, 153, 298
SsiI CCGC 5 cut(s) 5, 23, 172, 188, 401
SspMI CTAG 1 cut(s) 129
StyD4I CCNGG 3 cut(s) 208, 334, 432
StyI CCWWGG 1 cut(s) 278
TaaI ACNGT 3 cut(s) 38, 263, 478
TaqI TCGA 2 cut(s) 488, 534
TasI AATT 3 cut(s) 116, 153, 298
TatI WGTACW 2 cut(s) 30, 263
TauI GCSGC 2 cut(s) 175, 403
TfiI GAWTC 2 cut(s) 76, 136
Tru1I TTAA 2 cut(s) 62, 291
Tru9I TTAA 2 cut(s) 62, 291
TscAI CASTG 1 cut(s) 266
TseI GCWGC 4 cut(s) 125, 391, 403, 406
TspRI CASTG 1 cut(s) 266
VpaK11BI GGWCC 3 cut(s) 50, 282, 338
XapI RAATTY 1 cut(s) 298
XceI RCATGY 2 cut(s) 23, 71
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XspI CTAG 1 cut(s) 129
Zsp2I ATGCAT 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.