Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0012751

Physical Location & Seq

Genomic Coordinates Forward (+)

25539309 .. 25548426

Gene Structure 9118 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00186120.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3267 bp

ATGCCCTCCTTTTGTTTCTTCAACTTGGTCATCCTCGTCTGCTCATTGCCTGCTGCTTTGGGTGCCGACCCCTACGTGTTCTATGATTGGACCGTCTCCTACATCACTGCTGCTCCACTTGGAGTTAAGCAAAAGGTTATTGGGATTAATGGAGAGTTTCCAGGACCGTTGCTTAATGTGACTACAAATTGGAATGTTGTTATCAATGTGAAGAATGACCTGGATGAGCCATTGCTTCTCACATGGAATGGAATACAACATAGGAAAAACTGTTGGCAAGATGGTGTTTCGGGGACTAATTGTCCAATTCAGGCTGGTTGGAATTGGACATATGACTTTCAGGTTAAAGATCAGATAGGGAGTTTCTTTTATTTCCCTTCCCTAAGCTTCCAGAGAGCTGCAGGTGGATATGGGGGAATCATCATAAATAACAGAGCTATTATTCCGCTACCTTTTGCGGTGCCAGATGCAGATGTTACTCTCTTTATTACAGATTGGTATACAAAGAGTCATAAGGAACTACGGAAAGATGTTGAAAGTGGAACTAACCTAGGAATTCCTGACGGCATCCTCATCAACGGATTAGGTCCCTTTCGCTTTGATGAGGCAATTGTTCCAAACGGCTTTCCTTTCCTTACAATAAATGTTGAACCAGGAAAGACATACCGCTTCCGGGTGCACAACGTTGGCATCTCAACTAGCTTGAATTTCAGGATACAAAATCATAACTTACTCCTTGTGGAGACTGAAGGATCGTACACAGTTCAGCAGAACTATGCTAATATGGATATTCATGTTGGTCAGTCATTTTCATTCTTGGTTACAATGGATCAAAATGCCAGCAGTGATTACTACGTTGTTGCCAGCCCTCGTTTTGTGAATTCATCTTCATGGGCTAAAGCGACTGGAGTTGCCATCTTACACTACTCCAATTCTCAAGGACCTGCATCAGGTCCTCTCCCTGATCCTCCTAATGATTTCGACACATATTTCTCAATGAATCAAGCAAGATCTATAAGGTGGAATGTCTCTGCTGGTGCTGCTCGTCCAAACCCACAAGGTTCTTTTAAATATGGTGAAATTACCGTTACAGATTTCTACGTAATCCTAAACAGACCTGCAGAGCTTATAAATGGGAAGTGGCGCACAACCCTCAATGGTATTTCATATTTCACACCTTCGACACCGCTTAAGCTCGCCCAGCAATTCAACATTTCAGGAGTTTACAAGCTTGACTTTCCAAATAAACTGATGAACAGACCGGTGAAAATTGACACATCTTTAATAAATGGTACTTTCAAAGGTTTCATGGAAATCATCTTTCAGAACAATGATACTACTGTTCAGAACTACCATATGGATGGCTACGCATTTTTTGTCGTGGGTATGGATTTTGGAGTGTGGACAGAGAATAGCAGAGGCACTTACAACAAATGGGATGGTGTTGCTCGCTGCACTACACAGGTCTTTCCAGGAGCTTGGACAGCCATATTGATTTCTCTTGATAGTGCCGGCATTTGGAACCTTCGTGCAGAGAATCTGGACTCATGGTATCTAGGCCAAGAACTTTATGTCAGCGTTGTTAATCCTGAGAGTGATGGATCTGAAGCTGGTTTGCCTGATAATGCCATTTACTGTGGAGTACTTTCCTCTTTACAGAAAGATCAAGCTCAGCGAGTCAAGTTTTCCAGTTCACCTTCACTGTCCTGTTCGAGCACAACAATTTTGGTTCTTCTTCCCAAGTTGAGAGCTCAGTTTATCTCTTGGCTTGGAAGCAACGACAATCAGCAACTTGCAGAGACTAGTATTATTGTCTCCTACACAGTAGTATTATATTCACTGTTATCGTTCACGTTCTTGAGCTTGGTGCCAATCCAGTCAGCTCCTGAGGCAGCTCTCATAACCCAGATTCCTGGATTCAACGGCACTCTTCCTTCAAAGCACCGTTCTGGGTATGTGACGATTGATGAGAGTCATGGGAGGAACCTGTTTTACTATTTTGTGGAATCGGAGAGGAAGCCGTCGGAGGATCCGGTGGTGCTGTGGTTGAATGGTGGACCTGGTTGCTCCAGTTTTGATGGCTTTGTTTATGAGCATGGGCCATTCAATTTTGAAGCAGCAAACACCAAGGGAGGTCTGCCCAAATTGCACCTTAATCCATACAGCTGGTCAAAGGTTTCCAACATTTTATATTTGGATTCTCCTGCTGGTGTTGGGCTCTCTTACTCCAAAAACAAAAGTGACTATATTACTGGTGATCTAAAGACTGCATCTGATAGCCACACATTTCTCCTAAAGTGGTTTGAACTTTACCCAGAGTTCCTCTCCAACCCGTTTTTCATTTCGGGAGAGTCATATGCTGGTGTCTACGTGCCAACACTTGCTTTTGAAGTGGCCAAAGGAATTGATGCTGGTACAAAACCCATATTGAACTTCAAGGGGTATCTTGTGGGAAATGGAGTCACGGATGAGGAAATCGATGGGAATGCCCTTGTTCCATTTGCTCATGGGATGGGACTTATATCAGATGATTTATTTGAGGAGGTTATCGAGGAGTGCAATGGAAACTACTATCAGCCGAATAGTGATACTTGTGCGAACAAGCTTGAAAAAGTTGACAAGGCCATAGAAGGTTTGAACATTTATGACATTCTAGAACCATGCTATCACAGCACAGATTCTAAGAAAATTTCAACCGTAACCAGTAACTTACCATCTAGCTTTCGGAAGTTGGGTGAAACTCAAAGGCCTCTTGCTGTGAGAAAAAGGATGTTTGGCCGCGCATGGCCTCTTAGAGCTCCTGTGAGAGCTGGACTTGTTCCAACTTGGCCGCAGCTTATGAATAGTGAGGAAGTTCCATGCACTGATGATGAGGTTGCAACTGCATGGTTGAACAATGCAGCAGTTAGGAAGGCAATCCATGCAGAAGATGAAAGCGTGATCCCCAGTTGGGAATTATGTACAGACAACATTCGTTTTTACCATGATGCTGGGAGTATGATCCAGTACCATAAGAATCTGACTTCGAAGGGATACCGTGCGCTTATATATAGTGGTGATCATGATCTGTGTGTTCCATTCACCGGGAGTGAAGCATGGACTAGATCAGTTGGTTACAAGATTGTGGATGAATGGAGGCCATGGATTTCCCAGGGACAAATTGCTGGGCACGTATACACAAGGGGAGCTGGTCATACGGTTCCAGAATACAAACCTCAAGAGGCATTAGATTTGTATAGCCGATTCTTGGCTGGACAACCACTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0008150 GO:0008152 GO:0009505 GO:0009506 GO:0009653 GO:0009826 GO:0009932 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0016491 GO:0016722 GO:0030054 GO:0030312 GO:0031090 GO:0031224 GO:0031225 GO:0031226 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046658 GO:0048046 GO:0048589 GO:0048856 GO:0048869 GO:0055044 GO:0055114 GO:0060560 GO:0071840 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

1088

Amino Acids

120.92

Weight (kDa)

5.4

Isoelectric Point (pI)

35.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu-oxidase_3	PF07732	32 - 145	1.1e-41	Multicopper oxidase
Cu-oxidase	PF00394	158 - 312	1.1e-43	Multicopper oxidase
Cu-oxidase_2	PF07731	395 - 531	3e-29	Multicopper oxidase
Peptidase_S10	PF00450	638 - 1084	1.2e-131	Serine carboxypeptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012751)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48450 AT5G48450
fragaria_vesca	FvH4_2g17700 FvH4_2g17700
malus_domestica	MD05G1158300.v1.1
prunus_persica	Prupe.8G189700_v2.0.a1 Prupe.8G189700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0282691
rosa_laevigata	RLG00000012875
rosa_multiflora	Rmu_sc0003070.1_g000007 Rmu_sc0012099.1_g000005
rosa_roxburghii	Rroxscaffold_7G00186120
rosa_rugosa	Rorug06G0148600
rosa_samantha	Rh6AG262000 Rh6BG265100 Rh6CG264200 Rh6DG256500
rosa_wichuraiana	Rw6G022650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1130
AarI	CACCTGC	1 cut(s)	392
Acc36I	ACCTGC	3 cut(s)	392, 952, 1126
AccB1I	GGYRCC	3 cut(s)	62, 460, 1867
AccI	GTMKAC	3 cut(s)	500, 2367, 3174
AccII	CGCG	1 cut(s)	2781
AciI	CCGC	6 cut(s)	446, 458, 667, 1187, 2779, 2831
AclI	AACGTT	1 cut(s)	684
AclWI	GGATC	8 cut(s)	760, 837, 959, 1609, 2024, 2037, 2935, 2995
AcoI	YGGCCR	3 cut(s)	2394, 2776, 2828
AcsI	RAATTY	4 cut(s)	555, 706, 880, 2688
AcuI	CTGAAG	2 cut(s)	768, 1626
AfaI	GTAC	6 cut(s)	758, 1294, 1644, 2416, 2962, 3008
AfiI	CCNNNNNNNGG	6 cut(s)	673, 950, 1518, 2950, 3083, 3247
AflII	CTTAAG	1 cut(s)	1190
AflIII	ACRYGT	1 cut(s)	75
AgeI	ACCGGT	1 cut(s)	1261
AhdI	GACNNNNNGTC	1 cut(s)	300
AhlI	ACTAGT	1 cut(s)	1802
AjnI	CCWGG	7 cut(s)	160, 219, 652, 1471, 1912, 2059, 3150
AjuI	GAANNNNNNNTTGG	2 cut(s)	2321, 2353
Alw21I	GWGCWC	4 cut(s)	681, 1718, 1753, 2800
Alw26I	GTCTC	5 cut(s)	100, 737, 1033, 1793, 1819
Alw44I	GTGCAC	1 cut(s)	677
AlwI	GGATC	8 cut(s)	760, 837, 959, 1609, 2024, 2037, 2935, 2995
AlwNI	CAGNNNCTG	1 cut(s)	1886
AoxI	GGCC	9 cut(s)	1558, 2099, 2394, 2622, 2747, 2776, 2786, 2828, 3137
ApaLI	GTGCAC	1 cut(s)	677
ApeKI	GCWGC	9 cut(s)	53, 110, 398, 1040, 1452, 1892, 2117, 2833, 2900
ApoI	RAATTY	4 cut(s)	555, 706, 880, 2688
AseI	ATTAAT	1 cut(s)	147
AsiGI	ACCGGT	1 cut(s)	1261
AspA2I	CCTAGG	1 cut(s)	550
AspLEI	GCGC	3 cut(s)	1146, 2783, 3043
AspS9I	GGNCC	7 cut(s)	90, 164, 587, 941, 953, 2057, 2099
AsuC2I	CCSGG	2 cut(s)	674, 3085
AsuHPI	GGTGA	7 cut(s)	1088, 1276, 1686, 2267, 2747, 3068, 3073
AsuII	TTCGAA	1 cut(s)	3026
AvaII	GGWCC	6 cut(s)	90, 164, 587, 941, 953, 2057
AvrII	CCTAGG	1 cut(s)	550
AxyI	CCTNAGG	1 cut(s)	1887
BaeGI	GKGCMC	2 cut(s)	681, 3171
BaeI	ACNNNNGTAYC	2 cut(s)	1326, 1359
BalI	TGGCCA	1 cut(s)	2396
BamHI	GGATCC	1 cut(s)	2029
BanI	GGYRCC	3 cut(s)	62, 460, 1867
BanII	GRGCYC	3 cut(s)	1753, 2220, 2800
Bbv12I	GWGCWC	4 cut(s)	681, 1718, 1753, 2800
BbvI	GCAGC	9 cut(s)	40, 97, 385, 1027, 1439, 1904, 2129, 2845, 2912
BccI	CCATC	9 cut(s)	275, 923, 1355, 1433, 1592, 2072, 2474, 2506, 2722
BceAI	ACGGC	4 cut(s)	580, 637, 1939, 2005
BcgI	CGANNNNNNTGC	2 cut(s)	2468, 2502
BciT130I	CCWGG	7 cut(s)	162, 221, 654, 1473, 1914, 2061, 3152
BciVI	GTATCC	2 cut(s)	708, 3026
BclI	TGATCA	1 cut(s)	3058
BcnI	CCSGG	2 cut(s)	674, 3085
BcoDI	GTCTC	5 cut(s)	100, 737, 1033, 1793, 1819
BcuI	ACTAGT	1 cut(s)	1802
BfaI	CTAG	8 cut(s)	551, 699, 1556, 1803, 2654, 2718, 3102, 3265
BfmI	CTRYAG	2 cut(s)	399, 1119
BfrI	CTTAAG	1 cut(s)	1190
BfuAI	ACCTGC	3 cut(s)	392, 952, 1126
BfuI	GTATCC	2 cut(s)	708, 3026
BglII	AGATCT	1 cut(s)	1010
BlnI	CCTAGG	1 cut(s)	550
BlpI	GCTNAGC	1 cut(s)	1671
BmcAI	AGTACT	1 cut(s)	1644
Bme1390I	CCNGG	9 cut(s)	162, 221, 654, 674, 1473, 1914, 2061, 3085, 3152
Bme18I	GGWCC	6 cut(s)	90, 164, 587, 941, 953, 2057
BmeRI	GACNNNNNGTC	1 cut(s)	300
BmgT120I	GGNCC	7 cut(s)	90, 164, 587, 941, 953, 2057, 2099
BmiI	GGNNCC	8 cut(s)	64, 462, 589, 1523, 1869, 1985, 2031, 3201
BmrFI	CCNGG	9 cut(s)	162, 221, 654, 674, 1473, 1914, 2061, 3085, 3152
BmrI	ACTGGG	1 cut(s)	2940
BmsI	GCATC	7 cut(s)	457, 576, 699, 956, 2279, 2398, 2977
BmuI	ACTGGG	1 cut(s)	2940
BplI	GAGNNNNNCTC	2 cut(s)	2309, 2341
BpmI	CTGGAG	2 cut(s)	927, 2053
Bpu10I	CCTNAGC	1 cut(s)	383
Bpu1102I	GCTNAGC	1 cut(s)	1671
Bpu14I	TTCGAA	1 cut(s)	3026
BpuEI	CTTGAG	3 cut(s)	921, 1879, 3201
BpuMI	CCSGG	2 cut(s)	674, 3085
Bsa29I	ATCGAT	1 cut(s)	2478
BsaAI	YACGTR	4 cut(s)	76, 1102, 2371, 3172
BsaBI	GATNNNNATC	1 cut(s)	3063
BsaJI	CCNNGG	5 cut(s)	550, 2127, 3140, 3150, 3151
BsaWI	WCCGGW	2 cut(s)	1261, 2032
BsaXI	ACNNNNNCTCC	2 cut(s)	97, 127
Bsc4I	CCNNNNNNNGG	6 cut(s)	673, 950, 1518, 2950, 3083, 3247
Bse118I	RCCGGY	2 cut(s)	1261, 1511
Bse1I	ACTGG	8 cut(s)	910, 1689, 1876, 2070, 2257, 2703, 2946, 3004
Bse21I	CCTNAGG	1 cut(s)	1887
Bse3DI	GCAATG	3 cut(s)	44, 230, 2566
Bse8I	GATNNNNATC	1 cut(s)	3063
BseBI	CCWGG	7 cut(s)	162, 221, 654, 1473, 1914, 2061, 3152
BseCI	ATCGAT	1 cut(s)	2478
BseDI	CCNNGG	5 cut(s)	550, 2127, 3140, 3150, 3151
BseGI	GGATG	9 cut(s)	30, 229, 567, 1366, 1444, 2473, 2517, 2775, 3133
BseJI	GATNNNNATC	1 cut(s)	3063
BseLI	CCNNNNNNNGG	6 cut(s)	673, 950, 1518, 2950, 3083, 3247
BseMI	GCAATG	3 cut(s)	44, 230, 2566
BseMII	CTCAG	4 cut(s)	1581, 1685, 1766, 1878
BseNI	ACTGG	8 cut(s)	910, 1689, 1876, 2070, 2257, 2703, 2946, 3004
BseRI	GAGGAG	2 cut(s)	2555, 2567
BseSI	GKGCMC	2 cut(s)	681, 3171
BseXI	GCAGC	9 cut(s)	40, 97, 385, 1027, 1439, 1904, 2129, 2845, 2912
BseYI	CCCAGC	3 cut(s)	1200, 2990, 3164
BsgI	GTGCAG	2 cut(s)	1438, 1551
Bsh1236I	CGCG	1 cut(s)	2781
BshFI	GGCC	9 cut(s)	1560, 2101, 2396, 2624, 2749, 2778, 2788, 2830, 3139
BshNI	GGYRCC	3 cut(s)	62, 460, 1867
BshTI	ACCGGT	1 cut(s)	1261
BshVI	ATCGAT	1 cut(s)	2478
BsiHKAI	GWGCWC	4 cut(s)	681, 1718, 1753, 2800
BsiSI	CCGG	5 cut(s)	673, 1262, 1512, 2033, 3084
BslFI	GGGAC	4 cut(s)	307, 573, 2529, 3168
BslI	CCNNNNNNNGG	6 cut(s)	673, 950, 1518, 2950, 3083, 3247
BsmAI	GTCTC	5 cut(s)	100, 737, 1033, 1793, 1819
BsmBI	CGTCTC	1 cut(s)	100
BsmFI	GGGAC	4 cut(s)	307, 573, 2529, 3168
BsmI	GAATGC	1 cut(s)	2491
BsnI	GGCC	9 cut(s)	1560, 2101, 2396, 2624, 2749, 2778, 2788, 2830, 3139
Bsp119I	TTCGAA	1 cut(s)	3026
Bsp1286I	GDGCHC	6 cut(s)	681, 1718, 1753, 2220, 2800, 3171
Bsp1407I	TGTACA	1 cut(s)	2960
Bsp1720I	GCTNAGC	1 cut(s)	1671
Bsp19I	CCATGG	1 cut(s)	3140
BspACI	CCGC	6 cut(s)	446, 458, 667, 1187, 2779, 2831
BspANI	GGCC	9 cut(s)	1560, 2101, 2396, 2624, 2749, 2778, 2788, 2830, 3139
BspCNI	CTCAG	4 cut(s)	1582, 1684, 1765, 1879
BspDI	ATCGAT	1 cut(s)	2478
BspFNI	CGCG	1 cut(s)	2781
BspHI	TCATGA	1 cut(s)	3061
BspLI	GGNNCC	8 cut(s)	64, 462, 589, 1523, 1869, 1985, 2031, 3201
BspMAI	CTGCAG	2 cut(s)	403, 1123
BspMI	ACCTGC	3 cut(s)	392, 952, 1126
BspPI	GGATC	8 cut(s)	760, 837, 959, 1609, 2024, 2037, 2935, 2995
BspT104I	TTCGAA	1 cut(s)	3026
BspT107I	GGYRCC	3 cut(s)	62, 460, 1867
BspTI	CTTAAG	1 cut(s)	1190
BsrDI	GCAATG	3 cut(s)	44, 230, 2566
BsrFI	RCCGGY	2 cut(s)	1261, 1511
BsrGI	TGTACA	1 cut(s)	2960
BsrI	ACTGG	8 cut(s)	910, 1689, 1876, 2070, 2257, 2703, 2946, 3004
BssAI	RCCGGY	2 cut(s)	1261, 1511
BssECI	CCNNGG	5 cut(s)	550, 2127, 3140, 3150, 3151
BssNAI	GTATAC	2 cut(s)	501, 3175
BssT1I	CCWWGG	3 cut(s)	550, 2127, 3140
Bst1107I	GTATAC	2 cut(s)	501, 3175
Bst2UI	CCWGG	7 cut(s)	162, 221, 654, 1473, 1914, 2061, 3152
Bst6I	CTCTTC	1 cut(s)	1935
BstAFI	CTTAAG	1 cut(s)	1190
BstAPI	GCANNNNNTGC	1 cut(s)	2921
BstAUI	TGTACA	1 cut(s)	2960
BstBAI	YACGTR	4 cut(s)	76, 1102, 2371, 3172
BstBI	TTCGAA	1 cut(s)	3026
BstC8I	GCNNGC	6 cut(s)	51, 841, 865, 1197, 1450, 1513
BstDEI	CTNAG	7 cut(s)	383, 1590, 1671, 1752, 1887, 2682, 2792
BstDSI	CCRYGG	1 cut(s)	3140
BstENI	CCTNNNNNAGG	1 cut(s)	948
BstF5I	GGATG	9 cut(s)	30, 229, 567, 1366, 1444, 2473, 2517, 2775, 3133
BstFNI	CGCG	1 cut(s)	2781
BstHHI	GCGC	3 cut(s)	1146, 2783, 3043
BstMAI	GTCTC	5 cut(s)	100, 737, 1033, 1793, 1819
BstMWI	GCNNNNNNNGC	8 cut(s)	62, 1040, 1201, 1484, 1889, 2146, 2670, 2921
BstNI	CCWGG	7 cut(s)	162, 221, 654, 1473, 1914, 2061, 3152
BstSCI	CCNGG	9 cut(s)	160, 219, 652, 672, 1471, 1912, 2059, 3083, 3150
BstSFI	CTRYAG	2 cut(s)	399, 1119
BstSLI	GKGCMC	2 cut(s)	681, 3171
BstSNI	TACGTA	1 cut(s)	1102
BstUI	CGCG	1 cut(s)	2781
BstV1I	GCAGC	9 cut(s)	40, 97, 385, 1027, 1439, 1904, 2129, 2845, 2912
BstX2I	RGATCY	3 cut(s)	1010, 1601, 2029
BstXI	CCANNNNNNTGG	5 cut(s)	1361, 1479, 1913, 2166, 2990
BstYI	RGATCY	3 cut(s)	1010, 1601, 2029
BstZ17I	GTATAC	2 cut(s)	501, 3175
Bsu15I	ATCGAT	1 cut(s)	2478
Bsu36I	CCTNAGG	1 cut(s)	1887
BsuI	GTATCC	2 cut(s)	708, 3026
BsuRI	GGCC	9 cut(s)	1560, 2101, 2396, 2624, 2749, 2778, 2788, 2830, 3139
BsuTUI	ATCGAT	1 cut(s)	2478
BtgI	CCRYGG	1 cut(s)	3140
BtsCI	GGATG	9 cut(s)	30, 229, 567, 1366, 1444, 2473, 2517, 2775, 3133
BtsI	GCAGTG	2 cut(s)	105, 850
BtsIMutI	CAGTG	5 cut(s)	105, 850, 1700, 1838, 2862
BveI	ACCTGC	3 cut(s)	392, 952, 1126
Cac8I	GCNNGC	6 cut(s)	51, 841, 865, 1197, 1450, 1513
CaiI	CAGNNNCTG	1 cut(s)	1886
CciI	TCATGA	1 cut(s)	3061
CfoI	GCGC	3 cut(s)	1146, 2783, 3043
Cfr10I	RCCGGY	2 cut(s)	1261, 1511
Cfr13I	GGNCC	7 cut(s)	90, 164, 587, 941, 953, 2057, 2099
ClaI	ATCGAT	1 cut(s)	2478
CsiI	ACCWGGT	1 cut(s)	2059
Csp6I	GTAC	6 cut(s)	757, 1293, 1643, 2415, 2961, 3007
CspAI	ACCGGT	1 cut(s)	1261
CviQI	GTAC	6 cut(s)	757, 1293, 1643, 2415, 2961, 3007
DdeI	CTNAG	7 cut(s)	383, 1590, 1671, 1752, 1887, 2682, 2792
DraI	TTTAAA	1 cut(s)	1069
DriI	GACNNNNNGTC	1 cut(s)	300
EaeI	YGGCCR	3 cut(s)	2394, 2776, 2828
Eam1104I	CTCTTC	1 cut(s)	1935
Eam1105I	GACNNNNNGTC	1 cut(s)	300
EarI	CTCTTC	1 cut(s)	1935
Ecl136II	GAGCTC	2 cut(s)	1751, 2798
Eco105I	TACGTA	1 cut(s)	1102
Eco130I	CCWWGG	3 cut(s)	550, 2127, 3140
Eco147I	AGGCCT	1 cut(s)	2749
Eco24I	GRGCYC	3 cut(s)	1753, 2220, 2800
Eco47I	GGWCC	6 cut(s)	90, 164, 587, 941, 953, 2057
Eco53kI	GAGCTC	2 cut(s)	1751, 2798
Eco57I	CTGAAG	2 cut(s)	768, 1626
Eco81I	CCTNAGG	1 cut(s)	1887
EcoICRI	GAGCTC	2 cut(s)	1751, 2798
EcoNI	CCTNNNNNAGG	1 cut(s)	948
EcoO109I	RGGNCCY	3 cut(s)	587, 941, 953
EcoRI	GAATTC	2 cut(s)	555, 880
EcoRII	CCWGG	7 cut(s)	160, 219, 652, 1471, 1912, 2059, 3150
EcoT14I	CCWWGG	3 cut(s)	550, 2127, 3140
EcoT38I	GRGCYC	3 cut(s)	1753, 2220, 2800
ErhI	CCWWGG	3 cut(s)	550, 2127, 3140
Esp3I	CGTCTC	1 cut(s)	100
FalI	AAGNNNNNCTT	2 cut(s)	1220, 1252
FaqI	GGGAC	4 cut(s)	307, 573, 2529, 3168
FauNDI	CATATG	3 cut(s)	331, 1356, 2356
FbaI	TGATCA	1 cut(s)	3058
FblI	GTMKAC	3 cut(s)	500, 2367, 3174
FokI	GGATG	9 cut(s)	17, 236, 554, 1373, 1451, 2480, 2524, 2782, 3140
FriOI	GRGCYC	3 cut(s)	1753, 2220, 2800
FspBI	CTAG	8 cut(s)	551, 699, 1556, 1803, 2654, 2718, 3102, 3265
GlaI	GCGC	3 cut(s)	1145, 2782, 3042
GsaI	CCCAGC	3 cut(s)	1204, 2994, 3168
GsuI	CTGGAG	2 cut(s)	927, 2053
HaeIII	GGCC	9 cut(s)	1560, 2101, 2396, 2624, 2749, 2778, 2788, 2830, 3139
HapII	CCGG	5 cut(s)	673, 1262, 1512, 2033, 3084
HhaI	GCGC	3 cut(s)	1146, 2783, 3043
Hin6I	GCGC	3 cut(s)	1144, 2781, 3041
HinP1I	GCGC	3 cut(s)	1144, 2781, 3041
HincII	GTYRAC	1 cut(s)	2617
HindII	GTYRAC	1 cut(s)	2617
HindIII	AAGCTT	3 cut(s)	385, 1229, 2603
HpaII	CCGG	5 cut(s)	673, 1262, 1512, 2033, 3084
HphI	GGTGA	7 cut(s)	1088, 1276, 1686, 2267, 2747, 3068, 3073
Hpy99I	CGWCG	1 cut(s)	2026
HpyAV	CCTTC	9 cut(s)	387, 743, 1188, 1535, 1707, 1944, 2624, 2905, 3022
HpyCH4IV	ACGT	7 cut(s)	75, 684, 855, 1101, 1853, 2370, 3171
HpyF10VI	GCNNNNNNNGC	8 cut(s)	62, 1040, 1201, 1484, 1889, 2146, 2670, 2921
HpyF3I	CTNAG	7 cut(s)	383, 1590, 1671, 1752, 1887, 2682, 2792
HpySE526I	ACGT	7 cut(s)	75, 684, 855, 1101, 1853, 2370, 3171
HspAI	GCGC	3 cut(s)	1144, 2781, 3041
KroI	GCCGGC	1 cut(s)	1511
KroNI	GCCGGC	1 cut(s)	1513
Ksp22I	TGATCA	1 cut(s)	3058
LmnI	GCTCC	6 cut(s)	118, 1475, 1888, 2072, 2803, 3185
Lsp1109I	GCAGC	9 cut(s)	40, 97, 385, 1027, 1439, 1904, 2129, 2845, 2912
LweI	GCATC	7 cut(s)	457, 576, 699, 956, 2279, 2398, 2977
MabI	ACCWGGT	1 cut(s)	2059
MaeI	CTAG	8 cut(s)	551, 699, 1556, 1803, 2654, 2718, 3102, 3265
MaeII	ACGT	7 cut(s)	75, 684, 855, 1101, 1853, 2370, 3171
MboII	GAAGA	7 cut(s)	10, 223, 879, 1724, 1727, 1922, 2939
MfeI	CAATTG	1 cut(s)	609
MflI	RGATCY	3 cut(s)	1010, 1601, 2029
MhlI	GDGCHC	6 cut(s)	681, 1718, 1753, 2220, 2800, 3171
MlsI	TGGCCA	1 cut(s)	2396
MluNI	TGGCCA	1 cut(s)	2396
MlyI	GAGTC	6 cut(s)	517, 1538, 1686, 1981, 2360, 2469
MmeI	TCCRAC	5 cut(s)	299, 2004, 2205, 2352, 2846
Mox20I	TGGCCA	1 cut(s)	2396
MroNI	GCCGGC	1 cut(s)	1511
MscI	TGGCCA	1 cut(s)	2396
MseI	TTAA	9 cut(s)	126, 147, 174, 345, 1068, 1191, 1283, 1584, 2154
MslI	CAYNNNNRTG	2 cut(s)	889, 1359
Msp20I	TGGCCA	1 cut(s)	2396
MspA1I	CMGCKG	1 cut(s)	2166
MspCI	CTTAAG	1 cut(s)	1190
MspI	CCGG	5 cut(s)	673, 1262, 1512, 2033, 3084
MspR9I	CCNGG	9 cut(s)	162, 221, 654, 674, 1473, 1914, 2061, 3085, 3152
MunI	CAATTG	1 cut(s)	609
Mva1269I	GAATGC	1 cut(s)	2491
MvaI	CCWGG	7 cut(s)	162, 221, 654, 1473, 1914, 2061, 3152
MvnI	CGCG	1 cut(s)	2781
MwoI	GCNNNNNNNGC	8 cut(s)	62, 1040, 1201, 1484, 1889, 2146, 2670, 2921
NaeI	GCCGGC	1 cut(s)	1513
NciI	CCSGG	2 cut(s)	674, 3085
NcoI	CCATGG	1 cut(s)	3140
NdeI	CATATG	3 cut(s)	331, 1356, 2356
NgoMIV	GCCGGC	1 cut(s)	1511
NlaIV	GGNNCC	8 cut(s)	64, 462, 589, 1523, 1869, 1985, 2031, 3201
NmuCI	GTSAC	4 cut(s)	178, 1957, 2240, 2461
NspV	TTCGAA	1 cut(s)	3026
PagI	TCATGA	1 cut(s)	3061
PaqCI	CACCTGC	1 cut(s)	392
PasI	CCCWGGG	1 cut(s)	3151
PceI	AGGCCT	1 cut(s)	2749
PctI	GAATGC	1 cut(s)	2491
PdiI	GCCGGC	1 cut(s)	1513
PfoI	TCCNGGA	3 cut(s)	160, 1471, 1912
PinAI	ACCGGT	1 cut(s)	1261
PleI	GAGTC	6 cut(s)	516, 1538, 1685, 1980, 2359, 2468
PpsI	GAGTC	6 cut(s)	516, 1538, 1685, 1980, 2359, 2468
Ppu21I	YACGTR	4 cut(s)	76, 1102, 2371, 3172
PpuMI	RGGWCCY	3 cut(s)	587, 941, 953
PshBI	ATTAAT	1 cut(s)	147
PsiI	TTATAA	1 cut(s)	1130
Psp124BI	GAGCTC	2 cut(s)	1753, 2800
Psp1406I	AACGTT	1 cut(s)	684
Psp5II	RGGWCCY	3 cut(s)	587, 941, 953
Psp6I	CCWGG	7 cut(s)	160, 219, 652, 1471, 1912, 2059, 3150
PspFI	CCCAGC	3 cut(s)	1200, 2990, 3164
PspGI	CCWGG	7 cut(s)	160, 219, 652, 1471, 1912, 2059, 3150
PspN4I	GGNNCC	8 cut(s)	64, 462, 589, 1523, 1869, 1985, 2031, 3201
PspPI	GGNCC	7 cut(s)	90, 164, 587, 941, 953, 2057, 2099
PspPPI	RGGWCCY	3 cut(s)	587, 941, 953
PsrI	GAACNNNNNNTAC	2 cut(s)	1976, 2008
PstI	CTGCAG	2 cut(s)	403, 1123
PstNI	CAGNNNCTG	1 cut(s)	1886
PsuI	RGATCY	3 cut(s)	1010, 1601, 2029
PvuII	CAGCTG	1 cut(s)	2166
RsaI	GTAC	6 cut(s)	758, 1294, 1644, 2416, 2962, 3008
RsaNI	GTAC	6 cut(s)	757, 1293, 1643, 2415, 2961, 3007
RseI	CAYNNNNRTG	2 cut(s)	889, 1359
SacI	GAGCTC	2 cut(s)	1753, 2800
SaqAI	TTAA	9 cut(s)	126, 147, 174, 345, 1068, 1191, 1283, 1584, 2154
Sau96I	GGNCC	7 cut(s)	90, 164, 587, 941, 953, 2057, 2099
ScaI	AGTACT	1 cut(s)	1644
SchI	GAGTC	6 cut(s)	517, 1538, 1686, 1981, 2360, 2469
ScrFI	CCNGG	9 cut(s)	162, 221, 654, 674, 1473, 1914, 2061, 3085, 3152
SduI	GDGCHC	6 cut(s)	681, 1718, 1753, 2220, 2800, 3171
SexAI	ACCWGGT	1 cut(s)	2059
SfaNI	GCATC	7 cut(s)	457, 576, 699, 956, 2279, 2398, 2977
SfcI	CTRYAG	2 cut(s)	399, 1119
SfuI	TTCGAA	1 cut(s)	3026
SinI	GGWCC	6 cut(s)	90, 164, 587, 941, 953, 2057
SmiMI	CAYNNNNRTG	2 cut(s)	889, 1359
SmlI	CTYRAG	4 cut(s)	936, 1190, 1858, 3216
SmoI	CTYRAG	4 cut(s)	936, 1190, 1858, 3216
SnaBI	TACGTA	1 cut(s)	1102
SpeI	ACTAGT	1 cut(s)	1802
SseBI	AGGCCT	1 cut(s)	2749
SsiI	CCGC	6 cut(s)	446, 458, 667, 1187, 2779, 2831
SspMI	CTAG	8 cut(s)	551, 699, 1556, 1803, 2654, 2718, 3102, 3265
SstI	GAGCTC	2 cut(s)	1753, 2800
StuI	AGGCCT	1 cut(s)	2749
StyD4I	CCNGG	9 cut(s)	160, 219, 652, 672, 1471, 1912, 2059, 3083, 3150
StyI	CCWWGG	3 cut(s)	550, 2127, 3140
TaiI	ACGT	7 cut(s)	78, 687, 858, 1104, 1856, 2373, 3174
TaqI	TCGA	6 cut(s)	981, 1181, 1712, 2478, 2550, 3026
TatI	WGTACW	2 cut(s)	1642, 2960
TauI	GCSGC	2 cut(s)	2781, 2833
Tru1I	TTAA	9 cut(s)	126, 147, 174, 345, 1068, 1191, 1283, 1584, 2154
Tru9I	TTAA	9 cut(s)	126, 147, 174, 345, 1068, 1191, 1283, 1584, 2154
TscAI	CASTG	5 cut(s)	112, 850, 1707, 1845, 2869
TseFI	GTSAC	4 cut(s)	178, 1957, 2240, 2461
TseI	GCWGC	9 cut(s)	53, 110, 398, 1040, 1452, 1892, 2117, 2833, 2900
Tsp45I	GTSAC	4 cut(s)	178, 1957, 2240, 2461
TspGWI	ACGGA	3 cut(s)	538, 594, 2480
TspRI	CASTG	5 cut(s)	112, 850, 1707, 1845, 2869
Vha464I	CTTAAG	1 cut(s)	1190
VneI	GTGCAC	1 cut(s)	677
VpaK11BI	GGWCC	6 cut(s)	90, 164, 587, 941, 953, 2057
VspI	ATTAAT	1 cut(s)	147
XagI	CCTNNNNNAGG	1 cut(s)	948
XapI	RAATTY	4 cut(s)	555, 706, 880, 2688
XbaI	TCTAGA	1 cut(s)	2653
XmaJI	CCTAGG	1 cut(s)	550
XmiI	GTMKAC	3 cut(s)	500, 2367, 3174
XspI	CTAG	8 cut(s)	551, 699, 1556, 1803, 2654, 2718, 3102, 3265
ZrmI	AGTACT	1 cut(s)	1644

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.