Rroxscaffold_7G00207680

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0021454
Physical Location & Seq
Reverse (-)
57613155 .. 57614053
899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00207680.1

Sequence Viewer

Length: 576 bp
ATGGTATTAAGGTGGAGAGCAGCTCTTTCAGAAGCAGCAAATTTATCTGGGTGGCATTTCTCGGGCGGATATGAATATGAATTTATTGGTAAAATTGTTGAAGAGATTTCTGCACAAGTAAAAGAACCTACCTATTTGGATATGCCAAAGTGCCAAGTTGGGTTAGACTCTCGGGTAAAACATATACTTAAAATGTTAGATGTTGAAGGAAGTGATGTACGCATGGTAGGAATATGGGGAATTGGCGGAATAGGCAAAACAACAATTGCCAAAGCTATTTACAATACAATTGCCCATAAGTTTGATGGTAGCTGCTTTTTGGCAAATGTTAGAGAATATTCAGACCAACATGGAGGTTTAGTCGACCTACAAAATACTCTTCTCTCTACCATTGTGCGGCAGAAAGAATTGAAAATAAACAATGTTCATGAAGGAAACACTTTATTGCGGAACTGGATGATCACGAAGGTTGTGAGCTCTTCGACTTATATGCCTTCAAGAAAAAGAAAAATCATGATGATGATGAGAAATTCTGGATTAGCACTATTGTTAGATATGCTCATGGCCTTCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

191

Amino Acids

21.56

Weight (kDa)

9.37

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 58 - 156 4.7e-09 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021454)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0262671 RchiOBHm_Chr6g0262841
rosa_multiflora Rmu_sc0005237.1_g000020
rosa_roxburghii Rroxscaffold_7G00207680
rosa_wichuraiana Rw6G009780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 363
AciI CCGC 4 cut(s) 66, 246, 397, 448
AcsI RAATTY 3 cut(s) 40, 80, 529
AfaI GTAC 1 cut(s) 219
AfiI CCNNNNNNNGG 1 cut(s) 396
AgsI TTSAA 4 cut(s) 101, 206, 412, 498
AluBI AGCT 4 cut(s) 23, 275, 312, 477
AluI AGCT 4 cut(s) 23, 275, 312, 477
Alw21I GWGCWC 1 cut(s) 479
Ama87I CYCGRG 2 cut(s) 61, 171
AoxI GGCC 1 cut(s) 564
ApeKI GCWGC 3 cut(s) 20, 35, 312
ApoI RAATTY 3 cut(s) 40, 80, 529
AvaI CYCGRG 2 cut(s) 61, 171
BanII GRGCYC 1 cut(s) 479
Bbv12I GWGCWC 1 cut(s) 479
BbvI GCAGC 3 cut(s) 32, 47, 299
BccI CCATC 1 cut(s) 299
BcgI CGANNNNNNTGC 2 cut(s) 472, 506
BclI TGATCA 1 cut(s) 459
BisI GCNGC 4 cut(s) 21, 36, 313, 398
BlsI GCNGC 4 cut(s) 22, 37, 314, 399
BmeT110I CYCGRG 2 cut(s) 61, 171
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse1I ACTGG 1 cut(s) 458
BseGI GGATG 1 cut(s) 462
BseLI CCNNNNNNNGG 1 cut(s) 396
BseNI ACTGG 1 cut(s) 458
BseXI GCAGC 3 cut(s) 32, 47, 299
BsgI GTGCAG 1 cut(s) 96
BshFI GGCC 1 cut(s) 566
BsiHKAI GWGCWC 1 cut(s) 479
BsiHKCI CYCGRG 2 cut(s) 61, 171
BslI CCNNNNNNNGG 1 cut(s) 396
BsnI GGCC 1 cut(s) 566
BsoBI CYCGRG 2 cut(s) 61, 171
Bsp1286I GDGCHC 1 cut(s) 479
Bsp143I GATC 1 cut(s) 459
BspACI CCGC 4 cut(s) 66, 246, 397, 448
BspANI GGCC 1 cut(s) 566
BspHI TCATGA 2 cut(s) 427, 513
BspQI GCTCTTC 1 cut(s) 484
BsrI ACTGG 1 cut(s) 458
BssMI GATC 1 cut(s) 459
Bst6I CTCTTC 3 cut(s) 96, 384, 484
BstF5I GGATG 1 cut(s) 462
BstKTI GATC 1 cut(s) 462
BstMBI GATC 1 cut(s) 459
BstMWI GCNNNNNNNGC 1 cut(s) 252
BstV1I GCAGC 3 cut(s) 32, 47, 299
BsuRI GGCC 1 cut(s) 566
BtsCI GGATG 1 cut(s) 462
CciI TCATGA 2 cut(s) 427, 513
Csp6I GTAC 1 cut(s) 218
CviAII CATG 5 cut(s) 223, 350, 428, 514, 562
CviJI RGCY 5 cut(s) 23, 275, 312, 477, 566
CviKI_1 RGCY 5 cut(s) 23, 275, 312, 477, 566
CviQI GTAC 1 cut(s) 218
DpnI GATC 1 cut(s) 461
DpnII GATC 1 cut(s) 459
Eam1104I CTCTTC 3 cut(s) 96, 384, 484
EarI CTCTTC 3 cut(s) 96, 384, 484
EciI GGCGGA 2 cut(s) 81, 261
Ecl136II GAGCTC 1 cut(s) 477
Eco24I GRGCYC 1 cut(s) 479
Eco53kI GAGCTC 1 cut(s) 477
Eco88I CYCGRG 2 cut(s) 61, 171
EcoICRI GAGCTC 1 cut(s) 477
EcoT38I GRGCYC 1 cut(s) 479
FaeI CATG 5 cut(s) 226, 353, 431, 517, 565
FatI CATG 5 cut(s) 222, 349, 427, 513, 561
FbaI TGATCA 1 cut(s) 459
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 4 cut(s) 21, 36, 313, 398
FokI GGATG 1 cut(s) 469
FriOI GRGCYC 1 cut(s) 479
Fsp4HI GCNGC 4 cut(s) 21, 36, 313, 398
GluI GCNGC 4 cut(s) 21, 36, 313, 398
HaeIII GGCC 1 cut(s) 566
Hin1II CATG 5 cut(s) 226, 353, 431, 517, 565
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 1 cut(s) 167
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 2 cut(s) 31, 343
Hpy188III TCNNGA 5 cut(s) 428, 463, 498, 514, 534
Hpy8I GTNNAC 1 cut(s) 364
HpyAV CCTTC 4 cut(s) 200, 425, 460, 504
HpyCH4V TGCA 1 cut(s) 113
HpyF10VI GCNNNNNNNGC 1 cut(s) 252
Hsp92II CATG 5 cut(s) 226, 353, 431, 517, 565
Ksp22I TGATCA 1 cut(s) 459
Kzo9I GATC 1 cut(s) 459
LguI GCTCTTC 1 cut(s) 484
LpnPI CCDG 3 cut(s) 33, 439, 519
Lsp1109I GCAGC 3 cut(s) 32, 47, 299
MalI GATC 1 cut(s) 461
MboI GATC 1 cut(s) 459
MboII GAAGA 3 cut(s) 113, 371, 471
MfeI CAATTG 2 cut(s) 264, 288
MhlI GDGCHC 1 cut(s) 479
MluCI AATT 8 cut(s) 40, 80, 93, 240, 264, 288, 407, 529
MlyI GAGTC 1 cut(s) 161
MnlI CCTC 1 cut(s) 347
MseI TTAA 2 cut(s) 8, 189
MslI CAYNNNNRTG 1 cut(s) 518
MunI CAATTG 2 cut(s) 264, 288
MwoI GCNNNNNNNGC 1 cut(s) 252
NdeII GATC 1 cut(s) 459
NlaIII CATG 5 cut(s) 226, 353, 431, 517, 565
PagI TCATGA 2 cut(s) 427, 513
PciSI GCTCTTC 1 cut(s) 484
PkrI GCNGC 4 cut(s) 22, 37, 314, 399
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 479
RsaI GTAC 1 cut(s) 219
RsaNI GTAC 1 cut(s) 218
RseI CAYNNNNRTG 1 cut(s) 518
SacI GAGCTC 1 cut(s) 479
SalI GTCGAC 1 cut(s) 362
SapI GCTCTTC 1 cut(s) 484
SaqAI TTAA 2 cut(s) 8, 189
SatI GCNGC 4 cut(s) 21, 36, 313, 398
Sau3AI GATC 1 cut(s) 459
SchI GAGTC 1 cut(s) 161
SduI GDGCHC 1 cut(s) 479
SmiMI CAYNNNNRTG 1 cut(s) 518
Sse9I AATT 8 cut(s) 40, 80, 93, 240, 264, 288, 407, 529
SsiI CCGC 4 cut(s) 66, 246, 397, 448
SspI AATATT 1 cut(s) 338
SstI GAGCTC 1 cut(s) 479
TaqI TCGA 2 cut(s) 363, 482
TasI AATT 8 cut(s) 40, 80, 93, 240, 264, 288, 407, 529
TauI GCSGC 1 cut(s) 400
Tru1I TTAA 2 cut(s) 8, 189
Tru9I TTAA 2 cut(s) 8, 189
TseI GCWGC 3 cut(s) 20, 35, 312
TspDTI ATGAA 4 cut(s) 87, 93, 416, 444
TspGWI ACGGA 1 cut(s) 560
XapI RAATTY 3 cut(s) 40, 80, 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.