Rorug01G0452700

ankyrin repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0004727
Physical Location & Seq
Forward (+)
54766020 .. 54770118
4099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0452700.1

Sequence Viewer

Length: 1086 bp
ATGGCTTCCGATCTCGAAAAGCAGGTCAAGGAGGCCTTCATGGACGACCACTTCGAGCTCGCCGTTGACCTCTACACCCAGGCCATCGCCGTCGATCCCAACAACCCCGAGCTCTACTCCGACCGCGCTCAGGCCAACATCAAGGCCAAAAATTTCACAGAGGCAGTTGCGGATGCGAACAAGGCGATTGAATTGGACGAGTCTTTGTACAAGGCCTACTTGCGCAAGGGAATTGCCTGCCTGGAGCTCGAGGAATATCAGACTGCAAAGGCAGCATTGGAACTTGGTGCTTCTCTGGCTCCAGAGGAACCTAGATTCGCTAAACTGATAAAAGAGTGTGATGAGCTCATAGCAGAGGAAAATAACGAGCTACCAAAGCAACCAATGGAAACAACCACTACAGAGAGTGTTACAGCCGCAAAAGATGTTGAGCCTGTGAACCTGCCTTCCGATCAGGTGACAGTAGCACCTGTCAAACCAAAATACAGGCATGAATTCTACCAGACGCCTGATGAAGTGGTTTTGACTATATTTGCAAAGGGCATACCAGCCAAAGATGTTGCTGTTGAATTTGGTGAACAAATATTAAGTGTCAGCATTGATGTTCCTGGTGAAGATACATATCATTTTCAGCCTCGCCTGTTTGGGAAGATCATTCCTGAAAAGTGCAGATTTGATGTTTTGTCTACCAAGGTTGAAATTCGCCTGGCCAAAGCTGAAGCCATACAGTGGACATCTCTTGAGTTCAGCAAGGATAACCCTTTTCCTCTAAAGGTCAATGCCTCAGTTATTGGAGCCCAAAGGCCATCTTACCCATCCTCCAAACCAAAGCGGGTAGATTGGGACAAGCTTGAAGCCGACGTGAAGAAGGAGGAGAAAGATGAGAAGCTTGATGGCGATGCAGCTTTGAACAAATTTTTCCAAGACATATACAAGGATGCTGATGAGGACACAAGAAGGGCCATGAGAAAATCTTTTGTGGAGTCAAATGGGACGGTGCTTTCTACAAATTGGAAAGAAGTGGGCAACAAGAAGGTCGAGGGAAGTGCCCCTGATGGTATGGAGATGAAGAAATGGGAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

361

Amino Acids

40.65

Weight (kDa)

4.82

Isoelectric Point (pI)

31.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CS PF04969 163 - 238 9e-13 CS domain
SGS PF05002 280 - 359 1.3e-35 SGS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 224
Acc36I ACCTGC 2 cut(s) 13, 450
AccB7I CCANNNNNTGG 1 cut(s) 729
AccI GTMKAC 1 cut(s) 686
AccII CGCG 1 cut(s) 126
AciI CCGC 4 cut(s) 124, 170, 417, 832
AclWI GGATC 1 cut(s) 89
AcoI YGGCCR 1 cut(s) 708
AcsI RAATTY 5 cut(s) 151, 494, 569, 699, 914
AcuI CTGAAG 1 cut(s) 738
AcyI GRCGYC 1 cut(s) 506
AfaI GTAC 2 cut(s) 209, 1082
AfiI CCNNNNNNNGG 2 cut(s) 130, 729
AgsI TTSAA 5 cut(s) 191, 569, 698, 854, 910
AjiI CACGTC 1 cut(s) 862
AjnI CCWGG 4 cut(s) 78, 240, 607, 705
AloI GAACNNNNNNTCC 2 cut(s) 431, 463
AluBI AGCT 9 cut(s) 58, 112, 247, 346, 370, 716, 850, 889, 905
AluI AGCT 9 cut(s) 58, 112, 247, 346, 370, 716, 850, 889, 905
Alw21I GWGCWC 4 cut(s) 60, 114, 249, 348
AlwI GGATC 1 cut(s) 89
Ama87I CYCGRG 2 cut(s) 107, 248
AoxI GGCC 8 cut(s) 33, 81, 132, 144, 213, 708, 803, 960
ApeKI GCWGC 2 cut(s) 272, 902
ApoI RAATTY 5 cut(s) 151, 494, 569, 699, 914
ArsI GACNNNNNNTTYG 4 cut(s) 9, 35, 41, 67
AspLEI GCGC 2 cut(s) 128, 225
AspS9I GGNCC 1 cut(s) 960
AsuHPI GGTGA 3 cut(s) 469, 587, 623
AvaI CYCGRG 2 cut(s) 107, 248
BaeGI GKGCMC 1 cut(s) 1051
BalI TGGCCA 1 cut(s) 710
BanII GRGCYC 5 cut(s) 60, 114, 249, 348, 799
Bbv12I GWGCWC 4 cut(s) 60, 114, 249, 348
BbvI GCAGC 2 cut(s) 284, 914
BccI CCATC 5 cut(s) 92, 814, 823, 887, 1049
BceAI ACGGC 2 cut(s) 47, 74
BcgI CGANNNNNNTGC 2 cut(s) 1028, 1062
BciT130I CCWGG 4 cut(s) 80, 242, 609, 707
BfaI CTAG 1 cut(s) 312
BfmI CTRYAG 1 cut(s) 399
BfuAI ACCTGC 2 cut(s) 13, 450
BisI GCNGC 3 cut(s) 273, 417, 903
BlsI GCNGC 3 cut(s) 274, 418, 904
BmcAI AGTACT 1 cut(s) 1082
Bme1390I CCNGG 4 cut(s) 80, 242, 609, 707
BmeT110I CYCGRG 2 cut(s) 107, 248
BmgBI CACGTC 1 cut(s) 862
BmgT120I GGNCC 1 cut(s) 960
BmiI GGNNCC 3 cut(s) 300, 309, 796
BmrFI CCNGG 4 cut(s) 80, 242, 609, 707
BmsI GCATC 3 cut(s) 163, 889, 928
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpmI CTGGAG 2 cut(s) 263, 285
Bpu10I CCTNAGC 1 cut(s) 129
BpuEI CTTGAG 1 cut(s) 761
BsaBI GATNNNNATC 1 cut(s) 621
BsaHI GRCGYC 1 cut(s) 506
BsaJI CCNNGG 2 cut(s) 78, 690
BsaXI ACNNNNNCTCC 2 cut(s) 803, 833
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 729
Bse8I GATNNNNATC 1 cut(s) 621
BseBI CCWGG 4 cut(s) 80, 242, 609, 707
BseDI CCNNGG 2 cut(s) 78, 690
BseGI GGATG 3 cut(s) 178, 815, 943
BseJI GATNNNNATC 1 cut(s) 621
BseLI CCNNNNNNNGG 2 cut(s) 130, 729
BseMII CTCAG 2 cut(s) 143, 798
BseRI GAGGAG 1 cut(s) 887
BseSI GKGCMC 1 cut(s) 1051
BseXI GCAGC 2 cut(s) 284, 914
BsgI GTGCAG 1 cut(s) 688
Bsh1236I CGCG 1 cut(s) 126
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 8 cut(s) 35, 83, 134, 146, 215, 710, 805, 962
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 4 cut(s) 60, 114, 249, 348
BsiHKCI CYCGRG 2 cut(s) 107, 248
BslFI GGGAC 2 cut(s) 857, 1006
BslI CCNNNNNNNGG 2 cut(s) 130, 729
BsmFI GGGAC 2 cut(s) 857, 1006
BsnI GGCC 8 cut(s) 35, 83, 134, 146, 215, 710, 805, 962
BsoBI CYCGRG 2 cut(s) 107, 248
Bsp1286I GDGCHC 6 cut(s) 60, 114, 249, 348, 799, 1051
Bsp1407I TGTACA 1 cut(s) 207
Bsp143I GATC 4 cut(s) 10, 94, 451, 651
BspACI CCGC 4 cut(s) 124, 170, 417, 832
BspANI GGCC 8 cut(s) 35, 83, 134, 146, 215, 710, 805, 962
BspCNI CTCAG 2 cut(s) 142, 797
BspFNI CGCG 1 cut(s) 126
BspLI GGNNCC 3 cut(s) 300, 309, 796
BspMI ACCTGC 2 cut(s) 13, 450
BspPI GGATC 1 cut(s) 89
BsrGI TGTACA 1 cut(s) 207
BssECI CCNNGG 2 cut(s) 78, 690
BssMI GATC 4 cut(s) 10, 94, 451, 651
BssNI GRCGYC 1 cut(s) 506
BssT1I CCWWGG 1 cut(s) 690
Bst2UI CCWGG 4 cut(s) 80, 242, 609, 707
Bst4CI ACNGT 3 cut(s) 463, 729, 997
BstACI GRCGYC 1 cut(s) 506
BstAUI TGTACA 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 60, 238
BstDEI CTNAG 2 cut(s) 129, 784
BstF5I GGATG 3 cut(s) 178, 815, 943
BstFNI CGCG 1 cut(s) 126
BstHHI GCGC 2 cut(s) 128, 225
BstKTI GATC 4 cut(s) 13, 97, 454, 654
BstMBI GATC 4 cut(s) 10, 94, 451, 651
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 4 cut(s) 182, 272, 296, 376
BstNI CCWGG 4 cut(s) 80, 242, 609, 707
BstSCI CCNGG 4 cut(s) 78, 240, 607, 705
BstSFI CTRYAG 1 cut(s) 399
BstSLI GKGCMC 1 cut(s) 1051
BstUI CGCG 1 cut(s) 126
BstV1I GCAGC 2 cut(s) 284, 914
BsuRI GGCC 8 cut(s) 35, 83, 134, 146, 215, 710, 805, 962
BtgZI GCGATG 2 cut(s) 70, 912
BtrI CACGTC 1 cut(s) 862
BtsCI GGATG 3 cut(s) 178, 815, 943
BtsIMutI CAGTG 1 cut(s) 734
BveI ACCTGC 2 cut(s) 13, 450
Cac8I GCNNGC 2 cut(s) 60, 238
CfoI GCGC 2 cut(s) 128, 225
Cfr13I GGNCC 1 cut(s) 960
CseI GACGC 1 cut(s) 514
Csp6I GTAC 2 cut(s) 208, 1081
CviAII CATG 3 cut(s) 40, 491, 964
CviQI GTAC 2 cut(s) 208, 1081
DdeI CTNAG 2 cut(s) 129, 784
DpnI GATC 4 cut(s) 12, 96, 453, 653
DpnII GATC 4 cut(s) 10, 94, 451, 651
EaeI YGGCCR 1 cut(s) 708
Ecl136II GAGCTC 4 cut(s) 58, 112, 247, 346
Eco130I CCWWGG 1 cut(s) 690
Eco147I AGGCCT 2 cut(s) 35, 215
Eco24I GRGCYC 5 cut(s) 60, 114, 249, 348, 799
Eco53kI GAGCTC 4 cut(s) 58, 112, 247, 346
Eco57I CTGAAG 1 cut(s) 738
Eco88I CYCGRG 2 cut(s) 107, 248
EcoICRI GAGCTC 4 cut(s) 58, 112, 247, 346
EcoRI GAATTC 1 cut(s) 494
EcoRII CCWGG 4 cut(s) 78, 240, 607, 705
EcoT14I CCWWGG 1 cut(s) 690
EcoT38I GRGCYC 5 cut(s) 60, 114, 249, 348, 799
ErhI CCWWGG 1 cut(s) 690
FaeI CATG 3 cut(s) 43, 494, 967
FalI AAGNNNNNCTT 6 cut(s) 20, 52, 203, 235, 793, 825
FaqI GGGAC 2 cut(s) 857, 1006
FatI CATG 3 cut(s) 39, 490, 963
FauI CCCGC 1 cut(s) 825
FblI GTMKAC 1 cut(s) 686
Fnu4HI GCNGC 3 cut(s) 273, 417, 903
FokI GGATG 3 cut(s) 185, 802, 950
FriOI GRGCYC 5 cut(s) 60, 114, 249, 348, 799
Fsp4HI GCNGC 3 cut(s) 273, 417, 903
FspBI CTAG 1 cut(s) 312
FspI TGCGCA 1 cut(s) 224
GlaI GCGC 2 cut(s) 127, 224
GluI GCNGC 3 cut(s) 273, 417, 903
GsuI CTGGAG 2 cut(s) 263, 285
HaeIII GGCC 8 cut(s) 35, 83, 134, 146, 215, 710, 805, 962
HgaI GACGC 1 cut(s) 514
HhaI GCGC 2 cut(s) 128, 225
Hin1I GRCGYC 1 cut(s) 506
Hin1II CATG 3 cut(s) 43, 494, 967
Hin6I GCGC 2 cut(s) 126, 223
HinP1I GCGC 2 cut(s) 126, 223
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HindIII AAGCTT 2 cut(s) 848, 887
HinfI GANTC 3 cut(s) 200, 315, 983
HphI GGTGA 3 cut(s) 469, 587, 623
Hpy166II GTNNAC 5 cut(s) 67, 439, 578, 687, 732
Hpy188I TCNGA 4 cut(s) 10, 121, 261, 451
Hpy188III TCNNGA 4 cut(s) 14, 302, 659, 740
Hpy8I GTNNAC 5 cut(s) 67, 439, 578, 687, 732
Hpy99I CGWCG 2 cut(s) 95, 863
HpyAV CCTTC 5 cut(s) 46, 456, 862, 951, 1027
HpyCH4III ACNGT 3 cut(s) 463, 729, 997
HpyCH4IV ACGT 1 cut(s) 861
HpyCH4V TGCA 4 cut(s) 266, 536, 669, 902
HpyF10VI GCNNNNNNNGC 4 cut(s) 182, 272, 296, 376
HpyF3I CTNAG 2 cut(s) 129, 784
HpySE526I ACGT 1 cut(s) 861
Hsp92I GRCGYC 1 cut(s) 506
Hsp92II CATG 3 cut(s) 43, 494, 967
HspAI GCGC 2 cut(s) 126, 223
Kzo9I GATC 4 cut(s) 10, 94, 451, 651
LmnI GCTCC 3 cut(s) 244, 304, 794
Lsp1109I GCAGC 2 cut(s) 284, 914
LweI GCATC 3 cut(s) 163, 889, 928
MaeI CTAG 1 cut(s) 312
MaeII ACGT 1 cut(s) 861
MaeIII GTNAC 2 cut(s) 409, 457
MalI GATC 4 cut(s) 12, 96, 453, 653
MboI GATC 4 cut(s) 10, 94, 451, 651
MboII GAAGA 4 cut(s) 626, 661, 877, 1081
MhlI GDGCHC 6 cut(s) 60, 114, 249, 348, 799, 1051
MlsI TGGCCA 1 cut(s) 710
MluCI AATT 8 cut(s) 151, 191, 231, 494, 569, 699, 914, 1009
MluNI TGGCCA 1 cut(s) 710
MlyI GAGTC 2 cut(s) 209, 992
MmeI TCCRAC 1 cut(s) 144
Mox20I TGGCCA 1 cut(s) 710
MscI TGGCCA 1 cut(s) 710
MseI TTAA 1 cut(s) 587
Msp20I TGGCCA 1 cut(s) 710
MspR9I CCNGG 4 cut(s) 80, 242, 609, 707
MvaI CCWGG 4 cut(s) 80, 242, 609, 707
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 4 cut(s) 182, 272, 296, 376
NdeII GATC 4 cut(s) 10, 94, 451, 651
NlaIII CATG 3 cut(s) 43, 494, 967
NlaIV GGNNCC 3 cut(s) 300, 309, 796
NmuCI GTSAC 1 cut(s) 457
NsbI TGCGCA 1 cut(s) 224
PaeR7I CTCGAG 1 cut(s) 248
PceI AGGCCT 2 cut(s) 35, 215
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 60
PfeI GAWTC 1 cut(s) 315
PflMI CCANNNNNTGG 1 cut(s) 729
PkrI GCNGC 3 cut(s) 274, 418, 904
PleI GAGTC 2 cut(s) 208, 991
PpsI GAGTC 2 cut(s) 208, 991
Psp124BI GAGCTC 4 cut(s) 60, 114, 249, 348
Psp6I CCWGG 4 cut(s) 78, 240, 607, 705
PspGI CCWGG 4 cut(s) 78, 240, 607, 705
PspN4I GGNNCC 3 cut(s) 300, 309, 796
PspPI GGNCC 1 cut(s) 960
PspXI VCTCGAGB 1 cut(s) 248
RsaI GTAC 2 cut(s) 209, 1082
RsaNI GTAC 2 cut(s) 208, 1081
SacI GAGCTC 4 cut(s) 60, 114, 249, 348
SaqAI TTAA 1 cut(s) 587
SatI GCNGC 3 cut(s) 273, 417, 903
Sau3AI GATC 4 cut(s) 10, 94, 451, 651
Sau96I GGNCC 1 cut(s) 960
ScaI AGTACT 1 cut(s) 1082
SchI GAGTC 2 cut(s) 209, 992
ScrFI CCNGG 4 cut(s) 80, 242, 609, 707
SduI GDGCHC 6 cut(s) 60, 114, 249, 348, 799, 1051
SfaNI GCATC 3 cut(s) 163, 889, 928
SfcI CTRYAG 1 cut(s) 399
Sfr274I CTCGAG 1 cut(s) 248
SlaI CTCGAG 1 cut(s) 248
SmlI CTYRAG 2 cut(s) 248, 740
SmoI CTYRAG 2 cut(s) 248, 740
Sse9I AATT 8 cut(s) 151, 191, 231, 494, 569, 699, 914, 1009
SseBI AGGCCT 2 cut(s) 35, 215
SsiI CCGC 4 cut(s) 124, 170, 417, 832
SspI AATATT 1 cut(s) 585
SspMI CTAG 1 cut(s) 312
SstI GAGCTC 4 cut(s) 60, 114, 249, 348
StuI AGGCCT 2 cut(s) 35, 215
StyD4I CCNGG 4 cut(s) 78, 240, 607, 705
StyI CCWWGG 1 cut(s) 690
TaaI ACNGT 3 cut(s) 463, 729, 997
TaiI ACGT 1 cut(s) 864
TaqI TCGA 5 cut(s) 15, 54, 93, 249, 1038
TasI AATT 8 cut(s) 151, 191, 231, 494, 569, 699, 914, 1009
TatI WGTACW 2 cut(s) 207, 1080
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 1 cut(s) 315
Tru1I TTAA 1 cut(s) 587
Tru9I TTAA 1 cut(s) 587
TscAI CASTG 1 cut(s) 734
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 2 cut(s) 272, 902
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 4 cut(s) 28, 507, 528, 1082
TspRI CASTG 1 cut(s) 734
Van91I CCANNNNNTGG 1 cut(s) 729
XapI RAATTY 5 cut(s) 151, 494, 569, 699, 914
XhoI CTCGAG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 686
XspI CTAG 1 cut(s) 312
ZrmI AGTACT 1 cut(s) 1082
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.