Rorug02G0164700

Cysteine-rich TM module stress tolerance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0017581
Physical Location & Seq
Forward (+)
14671150 .. 14673221
2072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0164700.1

Sequence Viewer

Length: 1299 bp
ATGCCACAGCACCAAATGCGATCATCTACTCAAGAGCTTTTAAAGCTTGCTAAGTCAGCGAGCAAGCCTACAACAACAGCACAAGCAACAACATCCTCATTGCACTCATCAGTAGAGCAGATTCTCACTTCCAATCAGCCTTCTCAAGTTCGTTCTTCACCATCTGTGGATGAGGTCACTAACACTAGTGGTGGTGCAATTAGCAAGAAAGTGCGTGGTGAGACTCGATGTCTTGAGCTATCCAAGAGGAAGCGCGATGGTGTCCAATTTAATATTGATATTCCAAAGCATACCATGAGAGCTGTAGGAGCAAACTGCCAATTTTACATTACTGGTATGGGATGCTTCGTTCGAAAAAATGTTCCATTACAAATTAAGAAGTGGTCTGACATCTCAAGAGATGATGTTGCTTTGCTAATTCGCCATGTCCGTGAGAAATTCAATTTGAGCCATGAATCTCATGTGGATGAGGCAATTGAGAAACACATGATCAGATATTTTACCACATGGCGCTATAATTTGCATAAGAAGTTTCAGAAATATGAATCAGTAGAGGAAGCAATGGAGAATCGACCTGAAGATGTTGAAGAGGATGATTGGAACTACTTAATTGCAAATCTGTGGCAAGATCAAAAGTGGCTGAAAACAAGCGAAAAAAACAAGACAAACATGGACAAGTTAGAGATAACACATTGTGCAGGGACAAAGGCATTTAGTCGTCTTAGAACTGAAAATCGAAATCCTGAAACTGGAGAGGAAATTGGTCGTATTGACCTTTTCAAGCTCACACGATACAGTGAAAAGAAATCTGCATGGGTTGGTGATACAGCAAAGAATGCTTTTGAAGAGATGAAAAATCTACAAAACGAGCCACAAATGAATGAAAAATCTAGTGAAATAATGTATGAAGATGAGATCTATGATGAAGTGCTTTCCAAAATTGTTGGTCCACCACGATCAAGCTATATACGTGGCTTAGGAGTAGGCCCAAAGCCTAAAAGGTCTAAATTTTCTGCAAATGATGCTCAAGTGAGGGAGGCGAATCAAAGGGCAGATGAAGTGGAAAGAAGAGCAATTCAGCTTGCAGATGAATTGATGGCTGTAAAGTCTACTGCAGCTCAGCAAAATGAGGAGTTAGATATGGTCAAAGCTGGTGCAGCTCAACAAAATGAAGAGTTAGAGGCAGTGAAGGTTAGTGCGGCTCAACAAAATGAAGAGTTGGAGGCATTAAAGGCAAGACAAAATCAGACTGACACACTTTTACTCAAATTGATGGCGCAGTTGTCCTCCCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYSTM

Protein Analysis

432

Amino Acids

49.33

Weight (kDa)

7.66

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 197 - 332 9.7e-15 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017581)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18950
malus_domestica MD10G1057100.v1.1
prunus_persica Prupe.8G075700_v2.0.a1
pyrus_communis pycom10g04400
rosa_chinensis RchiOBHm_Chr2g0109971
rosa_multiflora Rmu_sc0028281.1_g000002
rosa_roxburghii Rroxscaffold_2G00133840
rosa_rugosa Rorug02G0164700
rosa_samantha Rh2AG216300 Rh2CG218500 Rh2DG221900
rosa_wichuraiana Rw2G016710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1109
AccII CGCG 1 cut(s) 255
AciI CCGC 1 cut(s) 1199
AcsI RAATTY 2 cut(s) 437, 1007
AcuI CTGAAG 1 cut(s) 597
AdeI CACNNNGTG 1 cut(s) 695
AfiI CCNNNNNNNGG 1 cut(s) 749
AgsI TTSAA 4 cut(s) 442, 587, 781, 845
AhdI GACNNNNNGTC 1 cut(s) 228
AhlI ACTAGT 1 cut(s) 185
AloI GAACNNNNNNTCC 2 cut(s) 333, 365
Alw26I GTCTC 1 cut(s) 215
AoxI GGCC 1 cut(s) 985
ApeKI GCWGC 2 cut(s) 1115, 1157
ApoI RAATTY 2 cut(s) 437, 1007
AspLEI GCGC 3 cut(s) 255, 513, 1279
AspS9I GGNCC 2 cut(s) 947, 986
AsuHPI GGTGA 3 cut(s) 150, 230, 833
AsuII TTCGAA 1 cut(s) 352
AvaII GGWCC 1 cut(s) 947
BbvI GCAGC 2 cut(s) 1127, 1169
BccI CCATC 4 cut(s) 169, 251, 1090, 1267
BclI TGATCA 1 cut(s) 489
BcoDI GTCTC 1 cut(s) 215
BcuI ACTAGT 1 cut(s) 185
BfaI CTAG 2 cut(s) 186, 891
BfmI CTRYAG 2 cut(s) 303, 1113
BfoI RGCGCY 1 cut(s) 514
BglII AGATCT 1 cut(s) 915
BisI GCNGC 3 cut(s) 1116, 1158, 1200
BlpI GCTNAGC 1 cut(s) 1119
BlsI GCNGC 3 cut(s) 1117, 1159, 1201
Bme18I GGWCC 1 cut(s) 947
BmeRI GACNNNNNGTC 1 cut(s) 228
BmgT120I GGNCC 2 cut(s) 947, 986
BmsI GCATC 2 cut(s) 332, 1012
BpmI CTGGAG 1 cut(s) 771
Bpu10I CCTNAGC 1 cut(s) 976
Bpu1102I GCTNAGC 1 cut(s) 1119
Bpu14I TTCGAA 1 cut(s) 352
BpuEI CTTGAG 5 cut(s) 15, 129, 254, 379, 1011
BsaAI YACGTR 1 cut(s) 971
Bsc4I CCNNNNNNNGG 1 cut(s) 749
Bse1I ACTGG 2 cut(s) 337, 754
Bse3DI GCAATG 2 cut(s) 98, 567
BseGI GGATG 5 cut(s) 92, 175, 347, 472, 598
BseLI CCNNNNNNNGG 1 cut(s) 749
BseMI GCAATG 2 cut(s) 98, 567
BseMII CTCAG 1 cut(s) 1133
BseNI ACTGG 2 cut(s) 337, 754
BseRI GAGGAG 1 cut(s) 1145
BseXI GCAGC 2 cut(s) 1127, 1169
BsgI GTGCAG 2 cut(s) 717, 1176
Bsh1236I CGCG 1 cut(s) 255
BshFI GGCC 1 cut(s) 987
BslFI GGGAC 1 cut(s) 715
BslI CCNNNNNNNGG 1 cut(s) 749
BsmAI GTCTC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 715
BsmI GAATGC 1 cut(s) 841
BsnI GGCC 1 cut(s) 987
Bsp119I TTCGAA 1 cut(s) 352
Bsp143I GATC 5 cut(s) 20, 489, 628, 915, 956
Bsp1720I GCTNAGC 1 cut(s) 1119
BspACI CCGC 1 cut(s) 1199
BspANI GGCC 1 cut(s) 987
BspCNI CTCAG 1 cut(s) 1132
BspFNI CGCG 1 cut(s) 255
BspMAI CTGCAG 1 cut(s) 1117
BspQI GCTCTTC 1 cut(s) 1063
BspT104I TTCGAA 1 cut(s) 352
BsrDI GCAATG 2 cut(s) 98, 567
BsrI ACTGG 2 cut(s) 337, 754
BssMI GATC 5 cut(s) 20, 489, 628, 915, 956
Bst4CI ACNGT 1 cut(s) 797
Bst6I CTCTTC 5 cut(s) 582, 840, 1063, 1167, 1209
BstAPI GCANNNNNTGC 3 cut(s) 16, 836, 1022
BstBAI YACGTR 1 cut(s) 971
BstBI TTCGAA 1 cut(s) 352
BstC8I GCNNGC 4 cut(s) 48, 61, 65, 1083
BstDEI CTNAG 4 cut(s) 51, 722, 976, 1119
BstF5I GGATG 5 cut(s) 92, 175, 347, 472, 598
BstFNI CGCG 1 cut(s) 255
BstH2I RGCGCY 1 cut(s) 514
BstHHI GCGC 3 cut(s) 255, 513, 1279
BstKTI GATC 5 cut(s) 23, 492, 631, 918, 959
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 5 cut(s) 20, 489, 628, 915, 956
BstMWI GCNNNNNNNGC 8 cut(s) 16, 43, 56, 308, 836, 1022, 1157, 1232
BstSFI CTRYAG 2 cut(s) 303, 1113
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 2 cut(s) 1127, 1169
BstX2I RGATCY 1 cut(s) 915
BstYI RGATCY 1 cut(s) 915
BsuRI GGCC 1 cut(s) 987
BtgZI GCGATG 1 cut(s) 270
BtsCI GGATG 5 cut(s) 92, 175, 347, 472, 598
BtsI GCAGTG 1 cut(s) 1191
BtsIMutI CAGTG 2 cut(s) 802, 1191
Cac8I GCNNGC 4 cut(s) 48, 61, 65, 1083
CfoI GCGC 3 cut(s) 255, 513, 1279
Cfr13I GGNCC 2 cut(s) 947, 986
CviAII CATG 8 cut(s) 295, 425, 452, 461, 487, 507, 670, 813
DdeI CTNAG 4 cut(s) 51, 722, 976, 1119
DpnI GATC 5 cut(s) 22, 491, 630, 917, 958
DpnII GATC 5 cut(s) 20, 489, 628, 915, 956
DraI TTTAAA 1 cut(s) 42
DraIII CACNNNGTG 1 cut(s) 695
DriI GACNNNNNGTC 1 cut(s) 228
Eam1104I CTCTTC 5 cut(s) 582, 840, 1063, 1167, 1209
Eam1105I GACNNNNNGTC 1 cut(s) 228
EarI CTCTTC 5 cut(s) 582, 840, 1063, 1167, 1209
Eco47I GGWCC 1 cut(s) 947
Eco57I CTGAAG 1 cut(s) 597
FaeI CATG 8 cut(s) 298, 428, 455, 464, 490, 510, 673, 816
FaqI GGGAC 1 cut(s) 715
FatI CATG 8 cut(s) 294, 424, 451, 460, 486, 506, 669, 812
FbaI TGATCA 1 cut(s) 489
FblI GTMKAC 1 cut(s) 1109
Fnu4HI GCNGC 3 cut(s) 1116, 1158, 1200
FokI GGATG 5 cut(s) 79, 182, 354, 479, 605
Fsp4HI GCNGC 3 cut(s) 1116, 1158, 1200
FspBI CTAG 2 cut(s) 186, 891
GlaI GCGC 3 cut(s) 254, 512, 1278
GluI GCNGC 3 cut(s) 1116, 1158, 1200
GsuI CTGGAG 1 cut(s) 771
HaeII RGCGCY 1 cut(s) 514
HaeIII GGCC 1 cut(s) 987
HhaI GCGC 3 cut(s) 255, 513, 1279
Hin1II CATG 8 cut(s) 298, 428, 455, 464, 490, 510, 673, 816
Hin6I GCGC 3 cut(s) 253, 511, 1277
HinP1I GCGC 3 cut(s) 253, 511, 1277
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 6 cut(s) 121, 223, 455, 545, 568, 1042
HphI GGTGA 3 cut(s) 150, 230, 833
Hpy166II GTNNAC 2 cut(s) 950, 1110
Hpy188I TCNGA 4 cut(s) 388, 494, 537, 1248
Hpy188III TCNNGA 4 cut(s) 32, 233, 396, 743
Hpy8I GTNNAC 2 cut(s) 950, 1110
HpyAV CCTTC 2 cut(s) 150, 1183
HpyCH4III ACNGT 1 cut(s) 797
HpyCH4IV ACGT 1 cut(s) 970
HpyF10VI GCNNNNNNNGC 8 cut(s) 16, 43, 56, 308, 836, 1022, 1157, 1232
HpyF3I CTNAG 4 cut(s) 51, 722, 976, 1119
HpySE526I ACGT 1 cut(s) 970
Hsp92II CATG 8 cut(s) 298, 428, 455, 464, 490, 510, 673, 816
HspAI GCGC 3 cut(s) 253, 511, 1277
Ksp22I TGATCA 1 cut(s) 489
Kzo9I GATC 5 cut(s) 20, 489, 628, 915, 956
LguI GCTCTTC 1 cut(s) 1063
LmnI GCTCC 1 cut(s) 308
LpnPI CCDG 6 cut(s) 318, 588, 684, 735, 756, 1137
Lsp1109I GCAGC 2 cut(s) 1127, 1169
LweI GCATC 2 cut(s) 332, 1012
MaeI CTAG 2 cut(s) 186, 891
MaeII ACGT 1 cut(s) 970
MaeIII GTNAC 1 cut(s) 175
MalI GATC 5 cut(s) 22, 491, 630, 917, 958
MboI GATC 5 cut(s) 20, 489, 628, 915, 956
MboII GAAGA 8 cut(s) 147, 590, 599, 857, 920, 1080, 1184, 1226
MfeI CAATTG 1 cut(s) 474
MflI RGATCY 1 cut(s) 915
MlyI GAGTC 1 cut(s) 217
MmeI TCCRAC 1 cut(s) 1200
MseI TTAA 5 cut(s) 41, 270, 375, 608, 1229
MslI CAYNNNNRTG 2 cut(s) 429, 465
MunI CAATTG 1 cut(s) 474
Mva1269I GAATGC 1 cut(s) 841
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 8 cut(s) 16, 43, 56, 308, 836, 1022, 1157, 1232
NdeII GATC 5 cut(s) 20, 489, 628, 915, 956
NlaIII CATG 8 cut(s) 298, 428, 455, 464, 490, 510, 673, 816
NmuCI GTSAC 1 cut(s) 175
NspV TTCGAA 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 1063
PcsI WCGNNNNNNNCGW 1 cut(s) 427
PctI GAATGC 1 cut(s) 841
PfeI GAWTC 5 cut(s) 121, 455, 545, 568, 1042
PkrI GCNGC 3 cut(s) 1117, 1159, 1201
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
Ppu21I YACGTR 1 cut(s) 971
PspPI GGNCC 2 cut(s) 947, 986
PstI CTGCAG 1 cut(s) 1117
PsuI RGATCY 1 cut(s) 915
RseI CAYNNNNRTG 2 cut(s) 429, 465
SapI GCTCTTC 1 cut(s) 1063
SaqAI TTAA 5 cut(s) 41, 270, 375, 608, 1229
SatI GCNGC 3 cut(s) 1116, 1158, 1200
Sau3AI GATC 5 cut(s) 20, 489, 628, 915, 956
Sau96I GGNCC 2 cut(s) 947, 986
SchI GAGTC 1 cut(s) 217
SfaNI GCATC 2 cut(s) 332, 1012
SfcI CTRYAG 2 cut(s) 303, 1113
SfuI TTCGAA 1 cut(s) 352
SinI GGWCC 1 cut(s) 947
SmiMI CAYNNNNRTG 2 cut(s) 429, 465
SmlI CTYRAG 5 cut(s) 30, 144, 233, 394, 1026
SmoI CTYRAG 5 cut(s) 30, 144, 233, 394, 1026
SpeI ACTAGT 1 cut(s) 185
SsiI CCGC 1 cut(s) 1199
SspI AATATT 1 cut(s) 274
SspMI CTAG 2 cut(s) 186, 891
TaaI ACNGT 1 cut(s) 797
TaiI ACGT 1 cut(s) 973
TaqI TCGA 4 cut(s) 226, 352, 571, 736
TauI GCSGC 1 cut(s) 1202
TfiI GAWTC 5 cut(s) 121, 455, 545, 568, 1042
Tru1I TTAA 5 cut(s) 41, 270, 375, 608, 1229
Tru9I TTAA 5 cut(s) 41, 270, 375, 608, 1229
TscAI CASTG 2 cut(s) 802, 1191
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 2 cut(s) 1115, 1157
Tsp45I GTSAC 1 cut(s) 175
TspGWI ACGGA 1 cut(s) 419
TspRI CASTG 2 cut(s) 802, 1191
VpaK11BI GGWCC 1 cut(s) 947
XapI RAATTY 2 cut(s) 437, 1007
XmiI GTMKAC 1 cut(s) 1109
XspI CTAG 2 cut(s) 186, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.