Rorug03G0159500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0008618
Physical Location & Seq
Forward (+)
13216719 .. 13217276
558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0159500.1

Sequence Viewer

Length: 558 bp
ATGACCTGCACAGCTCCTTGTAGATTTCAATTTTGCTGGTATTGCCTCGGACCATACGGTACATGCCATTGCAACGCGTTTAATAGGAGGGCAACTGATATGTCAGGGGGAGTTTGGGGCATGGCTGAGAAAGAGAGAATGCAGGCAAAGATAGATTTAGAGAGATATGCTCATTACTATGAACGTTGGGCAAACAATGGATCATCAAAGGAAAGAGCTCTCAAGGATTTTGATAAGGTGAAGACCGTGCACATTAAGGAACTTGGACACATACAGAAAAATACAATAGAGCATAAGTACGATTTTATCATAGAGGCCTGGCAACAGATAATTGAATGCAGGCAGGTATTGCGATGGACCTATGCTTATGGATACTACATGAAGGAGAATGAGGATGCCAAGCGTCATCTCTTCGAGCACTTGCAAGGCCATGCCGAGTTCAGCCTGGAGAGGCTTCACAAATGTGCAGAGTGTTGTAGTATACATGAAATTGTGGTAAAACTATTATGTCTATATCATGTAAATAGCATTATTATGGCTATGTATGATAGCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

21.98

Weight (kDa)

7.58

Isoelectric Point (pI)

63.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 14, 334
AccI GTMKAC 1 cut(s) 481
AccII CGCG 1 cut(s) 77
AclI AACGTT 1 cut(s) 184
AclWI GGATC 1 cut(s) 208
AfaI GTAC 2 cut(s) 61, 299
AflIII ACRYGT 1 cut(s) 75
AgsI TTSAA 2 cut(s) 29, 335
AjnI CCWGG 2 cut(s) 317, 444
AleI CACNNNNGTG 1 cut(s) 462
AluBI AGCT 2 cut(s) 14, 218
AluI AGCT 2 cut(s) 14, 218
Alw21I GWGCWC 3 cut(s) 220, 252, 420
Alw44I GTGCAC 1 cut(s) 248
AlwI GGATC 1 cut(s) 208
AoxI GGCC 2 cut(s) 315, 427
ApaLI GTGCAC 1 cut(s) 248
AspS9I GGNCC 2 cut(s) 50, 357
AsuHPI GGTGA 1 cut(s) 250
AvaII GGWCC 2 cut(s) 50, 357
BaeGI GKGCMC 1 cut(s) 252
BanII GRGCYC 1 cut(s) 220
BbsI GAAGAC 1 cut(s) 248
Bbv12I GWGCWC 3 cut(s) 220, 252, 420
BccI CCATC 1 cut(s) 348
BciT130I CCWGG 2 cut(s) 319, 446
BciVI GTATCC 1 cut(s) 365
BfuAI ACCTGC 2 cut(s) 14, 334
BfuI GTATCC 1 cut(s) 365
Bme1390I CCNGG 2 cut(s) 319, 446
Bme18I GGWCC 2 cut(s) 50, 357
BmgT120I GGNCC 2 cut(s) 50, 357
BmrFI CCNGG 2 cut(s) 319, 446
BmsI GCATC 1 cut(s) 385
BpiI GAAGAC 1 cut(s) 248
BplI GAGNNNNNCTC 2 cut(s) 154, 186
BpmI CTGGAG 1 cut(s) 467
BpuEI CTTGAG 1 cut(s) 206
BsaJI CCNNGG 1 cut(s) 46
Bse3DI GCAATG 1 cut(s) 67
BseBI CCWGG 2 cut(s) 319, 446
BseDI CCNNGG 1 cut(s) 46
BseGI GGATG 1 cut(s) 400
BseMI GCAATG 1 cut(s) 67
BseMII CTCAG 1 cut(s) 117
BseSI GKGCMC 1 cut(s) 252
BsgI GTGCAG 1 cut(s) 486
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 2 cut(s) 317, 429
BsiHKAI GWGCWC 3 cut(s) 220, 252, 420
BsmI GAATGC 2 cut(s) 144, 341
BsnI GGCC 2 cut(s) 317, 429
Bsp1286I GDGCHC 3 cut(s) 220, 252, 420
Bsp143I GATC 1 cut(s) 200
BspANI GGCC 2 cut(s) 317, 429
BspCNI CTCAG 1 cut(s) 118
BspFNI CGCG 1 cut(s) 77
BspMI ACCTGC 2 cut(s) 14, 334
BspPI GGATC 1 cut(s) 208
BsrDI GCAATG 1 cut(s) 67
BssECI CCNNGG 1 cut(s) 46
BssMI GATC 1 cut(s) 200
BssNAI GTATAC 1 cut(s) 482
Bst1107I GTATAC 1 cut(s) 482
Bst2UI CCWGG 2 cut(s) 319, 446
Bst4CI ACNGT 2 cut(s) 59, 247
Bst6I CTCTTC 1 cut(s) 416
BstAPI GCANNNNNTGC 1 cut(s) 349
BstC8I GCNNGC 2 cut(s) 144, 341
BstDEI CTNAG 1 cut(s) 126
BstF5I GGATG 1 cut(s) 400
BstFNI CGCG 1 cut(s) 77
BstKTI GATC 1 cut(s) 203
BstMBI GATC 1 cut(s) 200
BstMWI GCNNNNNNNGC 2 cut(s) 42, 349
BstNI CCWGG 2 cut(s) 319, 446
BstNSI RCATGY 1 cut(s) 66
BstSCI CCNGG 2 cut(s) 317, 444
BstSLI GKGCMC 1 cut(s) 252
BstUI CGCG 1 cut(s) 77
BstV2I GAAGAC 1 cut(s) 248
BstZ17I GTATAC 1 cut(s) 482
BsuI GTATCC 1 cut(s) 365
BsuRI GGCC 2 cut(s) 317, 429
BtgZI GCGATG 1 cut(s) 367
BtsCI GGATG 1 cut(s) 400
BveI ACCTGC 2 cut(s) 14, 334
Cac8I GCNNGC 2 cut(s) 144, 341
Cfr13I GGNCC 2 cut(s) 50, 357
CseI GACGC 1 cut(s) 392
Csp6I GTAC 2 cut(s) 60, 298
CviAII CATG 6 cut(s) 63, 121, 379, 431, 485, 518
CviJI RGCY 8 cut(s) 14, 125, 218, 317, 429, 444, 454, 539
CviKI_1 RGCY 8 cut(s) 14, 125, 218, 317, 429, 444, 454, 539
CviQI GTAC 2 cut(s) 60, 298
DdeI CTNAG 1 cut(s) 126
DpnI GATC 1 cut(s) 202
DpnII GATC 1 cut(s) 200
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
Ecl136II GAGCTC 1 cut(s) 218
Eco147I AGGCCT 1 cut(s) 317
Eco24I GRGCYC 1 cut(s) 220
Eco47I GGWCC 2 cut(s) 50, 357
Eco53kI GAGCTC 1 cut(s) 218
EcoICRI GAGCTC 1 cut(s) 218
EcoRII CCWGG 2 cut(s) 317, 444
EcoT38I GRGCYC 1 cut(s) 220
FaeI CATG 6 cut(s) 66, 124, 382, 434, 488, 521
FatI CATG 6 cut(s) 62, 120, 378, 430, 484, 517
FblI GTMKAC 1 cut(s) 481
FokI GGATG 1 cut(s) 407
FriOI GRGCYC 1 cut(s) 220
GsuI CTGGAG 1 cut(s) 467
HaeIII GGCC 2 cut(s) 317, 429
HgaI GACGC 1 cut(s) 392
Hin1II CATG 6 cut(s) 66, 124, 382, 434, 488, 521
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 2 cut(s) 250, 482
Hpy188I TCNGA 1 cut(s) 50
Hpy8I GTNNAC 2 cut(s) 250, 482
HpyAV CCTTC 1 cut(s) 376
HpyCH4III ACNGT 2 cut(s) 59, 247
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 7 cut(s) 9, 72, 142, 250, 339, 424, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 349
HpyF3I CTNAG 1 cut(s) 126
HpySE526I ACGT 1 cut(s) 184
Hsp92II CATG 6 cut(s) 66, 124, 382, 434, 488, 521
Kzo9I GATC 1 cut(s) 200
LmnI GCTCC 1 cut(s) 19
LweI GCATC 1 cut(s) 385
MaeII ACGT 1 cut(s) 184
MalI GATC 1 cut(s) 202
MboI GATC 1 cut(s) 200
MboII GAAGA 2 cut(s) 253, 403
MhlI GDGCHC 3 cut(s) 220, 252, 420
MluCI AATT 3 cut(s) 29, 330, 489
MluI ACGCGT 1 cut(s) 75
MnlI CCTC 5 cut(s) 56, 81, 307, 385, 444
MseI TTAA 3 cut(s) 81, 255, 556
MslI CAYNNNNRTG 3 cut(s) 177, 462, 533
MspR9I CCNGG 2 cut(s) 319, 446
Mva1269I GAATGC 2 cut(s) 144, 341
MvaI CCWGG 2 cut(s) 319, 446
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 2 cut(s) 42, 349
NdeII GATC 1 cut(s) 200
NlaIII CATG 6 cut(s) 66, 124, 382, 434, 488, 521
NmeAIII GCCGAG 1 cut(s) 460
NspI RCATGY 1 cut(s) 66
OliI CACNNNNGTG 1 cut(s) 462
PceI AGGCCT 1 cut(s) 317
PctI GAATGC 2 cut(s) 144, 341
Psp124BI GAGCTC 1 cut(s) 220
Psp1406I AACGTT 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 317, 444
PspGI CCWGG 2 cut(s) 317, 444
PspPI GGNCC 2 cut(s) 50, 357
RsaI GTAC 2 cut(s) 61, 299
RsaNI GTAC 2 cut(s) 60, 298
RseI CAYNNNNRTG 3 cut(s) 177, 462, 533
SacI GAGCTC 1 cut(s) 220
SaqAI TTAA 3 cut(s) 81, 255, 556
Sau3AI GATC 1 cut(s) 200
Sau96I GGNCC 2 cut(s) 50, 357
ScrFI CCNGG 2 cut(s) 319, 446
SduI GDGCHC 3 cut(s) 220, 252, 420
SetI ASST 7 cut(s) 8, 16, 187, 220, 240, 348, 362
SfaNI GCATC 1 cut(s) 385
SinI GGWCC 2 cut(s) 50, 357
SmiMI CAYNNNNRTG 3 cut(s) 177, 462, 533
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 3 cut(s) 29, 330, 489
SseBI AGGCCT 1 cut(s) 317
SstI GAGCTC 1 cut(s) 220
StuI AGGCCT 1 cut(s) 317
StyD4I CCNGG 2 cut(s) 317, 444
TaaI ACNGT 2 cut(s) 59, 247
TaiI ACGT 1 cut(s) 187
TaqI TCGA 1 cut(s) 414
TasI AATT 3 cut(s) 29, 330, 489
Tru1I TTAA 3 cut(s) 81, 255, 556
Tru9I TTAA 3 cut(s) 81, 255, 556
TspDTI ATGAA 3 cut(s) 195, 395, 501
VneI GTGCAC 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 50, 357
XceI RCATGY 1 cut(s) 66
XmiI GTMKAC 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.