Rorug03G0176800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0021672
Physical Location & Seq
Reverse (-)
15017333 .. 15019394
2062 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0176800.1

Sequence Viewer

Length: 1695 bp
ATGGTGCCCAAATGTCTCAGCTTCCATCTCCATCCCTCCTTTCCTGCAATAACCAAAAACCCAACTAGCTTTCTGACCTCTCCTTTTTTGCCAACTAACTTAACTGCCAAGTTCCACACCACCCATGTTCAAGCTCATCATCATCTTACTCTTAGAAGCCCCATTTTCACCACCATGGCTTCAATCCAATCCACTCCTAGTCCCAAATTCGATGACTTTGAAGCCGATCCCACTCTCACCAACGACGATCTCAAGCCCACAACGCCGGACCAGAGGAGTTTTTCCCGGTGGGAATTGGCTAGTCTTTGGGTTGGCCTTGTTGTGGGAGTGCCAACTTACTACCTGGCTGGTAGTCTAGTTGACCTTGGAATGGCTTGGTGGCAAGGAATTGCCACAGTTGTAGCAGCCAACATAGTACTACTAGTCCCACTGATCCTCACCGGTCATCCGGGCACTAAATATGGCATCTCTTTCCCGGTTTTGGCTAGATCCTCATTTGGGATCCATGGTGCTCATATCCCAACTCTTCTTAGAGCCTTGGTAGGTTGTGGTTGGTATGGAATTGAGACGTGGATCGGTGGTGAAGCAATTTTCCTTCTACTACCAAGTTCAATCAAAAGTTCCTCTTTGTCTCAATTCCTGCCACGGCTAGGCACATCCCCATTAGAGTTTGCTTGTTTTATTGTCTTTTGGGTTGCCCAATCGGCCATTGTTTGGAAAGGCATGGATGGAATCAGAGAGCTTGAGAAATACTCTGCTCCAATTCTAATCACACTCACGTCCTGCCTTGTAATTTGGGCTTATGTCAAAGCTGGTGGTTTTAGCCACATGCTTTCCATATCCTCCAAACTAACATCCCAACAATTTTGGTCCCTCTTCTTTCCTTCCCTCACTGCAAACATAAGCTTCTGGGCTACTGTTGCTCTCAACATACCCGATTTTACCCGATACGCCAAGAGCCAGACTGATCAGGTCATCGGTCAGGCGGGGCTGCCAATCTTCATGGGGGCATTCACATTCGTTGGCCTAGCTGTAACCTGCTCCACTGAGGTAATTTTTGGGCGTGTCATATCCAATCCAATCCAACTTCTTGGTGAGATCGGAGGGGTAACAACAATATTCCTAGCCATTTTTGGTGTTAGCCTTGCTACCTTTACCACCAACATTGCAGCTAATGTTGTGGCACCAGCAAATGCGCTAGTCAATCTTAGTCCTTCGAAGTTCACATTCAGAAGGGGAGCTATTTTCACAGCTTTGCTTGGAATTGCATTTCAGCCATGGAGGCTTCTTAGATCAAGTGAGAGCTTTGTCTACACATGGCTTGTTGGATATTCTGCTCTGTTGGGTCCAATTGGAGGCATAATTCTCGCAGATTACTATCTTGTCCACCACACAACCTTAAGCATGAAGGACTTGTACACACAAAGTCCTTATGGCGCTTACTACTATACGGGAGGCTACAATTTGGCAGCAATGGCAGCTCTTGTGTTTGGGATTTTGCCCGTGGTTCCAGGTTTCTTGCAAAAAGTTGGTGTTCTTTCTTCAGTATCACATACATTTGTGATCATCTACAACAATGCCTGGTTTGTTAGCTTCTTCTCTGCTGGTTTTCTCTATTGGCTTCTGTTATCTTTGAAAAGAAAACAGAACAAGTCTCTCGCTTCAGAAGACCCACTATTGCCTCCTGCAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

564

Amino Acids

61.57

Weight (kDa)

9.11

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transp_cyt_pur PF02133 88 - 530 8.7e-101 Permease for cytosine/purines, uracil, thiamine, allantoin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021672)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8311737.1_g000001 Rmu_sc0005609.1_g000008
rosa_rugosa Rorug03G0176700 Rorug03G0176700 Rorug03G0176800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1046
AccB1I GGYRCC 2 cut(s) 4, 1183
AccB7I CCANNNNNTGG 1 cut(s) 714
AccI GTMKAC 1 cut(s) 1311
AciI CCGC 1 cut(s) 986
AclWI GGATC 6 cut(s) 221, 427, 483, 496, 509, 581
AcoI YGGCCR 1 cut(s) 705
AcsI RAATTY 1 cut(s) 206
AcuI CTGAAG 2 cut(s) 1527, 1647
AfaI GTAC 2 cut(s) 417, 1418
AfiI CCNNNNNNNGG 7 cut(s) 322, 370, 481, 498, 650, 714, 1355
AflII CTTAAG 1 cut(s) 1399
AgeI ACCGGT 1 cut(s) 440
AgsI TTSAA 5 cut(s) 131, 183, 221, 612, 1636
AhlI ACTAGT 1 cut(s) 421
AjiI CACGTC 2 cut(s) 570, 780
AjnI CCWGG 3 cut(s) 342, 1510, 1580
Alw21I GWGCWC 1 cut(s) 514
Alw26I GTCTC 4 cut(s) 20, 560, 636, 1659
AlwI GGATC 6 cut(s) 221, 427, 483, 496, 509, 581
AoxI GGCC 3 cut(s) 313, 705, 1024
ApeKI GCWGC 5 cut(s) 404, 991, 1169, 1469, 1478
ApoI RAATTY 1 cut(s) 206
AsiGI ACCGGT 1 cut(s) 440
AspLEI GCGC 2 cut(s) 1198, 1439
AspS9I GGNCC 3 cut(s) 268, 870, 1346
AsuC2I CCSGG 3 cut(s) 286, 450, 476
AsuHPI GGTGA 5 cut(s) 160, 229, 430, 593, 1106
AsuII TTCGAA 1 cut(s) 1217
AvaII GGWCC 3 cut(s) 268, 870, 1346
BaeGI GKGCMC 2 cut(s) 9, 455
BamHI GGATCC 1 cut(s) 501
BanI GGYRCC 2 cut(s) 4, 1183
BarI GAAGNNNNNNTAC 2 cut(s) 1400, 1432
BbsI GAAGAC 1 cut(s) 1674
Bbv12I GWGCWC 1 cut(s) 514
BbvI GCAGC 5 cut(s) 416, 978, 1181, 1481, 1490
BccI CCATC 3 cut(s) 33, 39, 722
BceAI ACGGC 1 cut(s) 662
BcgI CGANNNNNNTGC 2 cut(s) 1348, 1382
BciT130I CCWGG 3 cut(s) 344, 1512, 1582
BclI TGATCA 2 cut(s) 967, 1563
BcnI CCSGG 3 cut(s) 286, 450, 476
BcoDI GTCTC 4 cut(s) 20, 560, 636, 1659
BcuI ACTAGT 1 cut(s) 421
BfoI RGCGCY 1 cut(s) 1440
BfrI CTTAAG 1 cut(s) 1399
BfuAI ACCTGC 1 cut(s) 1046
BglI GCCNNNNNGGC 2 cut(s) 704, 1282
BisI GCNGC 5 cut(s) 405, 992, 1170, 1470, 1479
BlsI GCNGC 5 cut(s) 406, 993, 1171, 1471, 1480
BmcAI AGTACT 1 cut(s) 417
Bme1390I CCNGG 6 cut(s) 286, 344, 450, 476, 1512, 1582
Bme18I GGWCC 3 cut(s) 268, 870, 1346
BmgBI CACGTC 2 cut(s) 570, 780
BmgT120I GGNCC 3 cut(s) 268, 870, 1346
BmiI GGNNCC 6 cut(s) 6, 503, 872, 1185, 1347, 1509
BmrFI CCNGG 6 cut(s) 286, 344, 450, 476, 1512, 1582
BmsI GCATC 1 cut(s) 474
BpiI GAAGAC 1 cut(s) 1674
BplI GAGNNNNNCTC 2 cut(s) 737, 769
Bpu14I TTCGAA 1 cut(s) 1217
BpuEI CTTGAG 2 cut(s) 236, 764
BpuMI CCSGG 3 cut(s) 286, 450, 476
BsaBI GATNNNNATC 1 cut(s) 1377
BsaJI CCNNGG 7 cut(s) 174, 364, 505, 537, 644, 1277, 1503
BsaWI WCCGGW 1 cut(s) 440
Bsc4I CCNNNNNNNGG 7 cut(s) 322, 370, 481, 498, 650, 714, 1355
Bse118I RCCGGY 1 cut(s) 440
Bse3DI GCAATG 2 cut(s) 1164, 1479
Bse8I GATNNNNATC 1 cut(s) 1377
BseBI CCWGG 3 cut(s) 344, 1512, 1582
BseDI CCNNGG 7 cut(s) 174, 364, 505, 537, 644, 1277, 1503
BseGI GGATG 5 cut(s) 31, 445, 656, 733, 854
BseJI GATNNNNATC 1 cut(s) 1377
BseLI CCNNNNNNNGG 7 cut(s) 322, 370, 481, 498, 650, 714, 1355
BseMI GCAATG 2 cut(s) 1164, 1479
BseMII CTCAG 2 cut(s) 31, 1038
BseRI GAGGAG 1 cut(s) 289
BseSI GKGCMC 2 cut(s) 9, 455
BseXI GCAGC 5 cut(s) 416, 978, 1181, 1481, 1490
BshFI GGCC 3 cut(s) 315, 707, 1026
BshNI GGYRCC 2 cut(s) 4, 1183
BshTI ACCGGT 1 cut(s) 440
BsiHKAI GWGCWC 1 cut(s) 514
BsiSI CCGG 5 cut(s) 266, 286, 441, 449, 476
BslFI GGGAC 3 cut(s) 186, 410, 856
BslI CCNNNNNNNGG 7 cut(s) 322, 370, 481, 498, 650, 714, 1355
BsmAI GTCTC 4 cut(s) 20, 560, 636, 1659
BsmBI CGTCTC 1 cut(s) 560
BsmFI GGGAC 3 cut(s) 186, 410, 856
BsmI GAATGC 1 cut(s) 1010
BsnI GGCC 3 cut(s) 315, 707, 1026
Bsp119I TTCGAA 1 cut(s) 1217
Bsp1286I GDGCHC 3 cut(s) 9, 455, 514
Bsp1407I TGTACA 1 cut(s) 1416
Bsp19I CCATGG 3 cut(s) 174, 505, 1277
BspACI CCGC 1 cut(s) 986
BspANI GGCC 3 cut(s) 315, 707, 1026
BspCNI CTCAG 2 cut(s) 30, 1039
BspLI GGNNCC 6 cut(s) 6, 503, 872, 1185, 1347, 1509
BspMI ACCTGC 1 cut(s) 1046
BspPI GGATC 6 cut(s) 221, 427, 483, 496, 509, 581
BspT104I TTCGAA 1 cut(s) 1217
BspT107I GGYRCC 2 cut(s) 4, 1183
BspTI CTTAAG 1 cut(s) 1399
BsrDI GCAATG 2 cut(s) 1164, 1479
BsrFI RCCGGY 1 cut(s) 440
BsrGI TGTACA 1 cut(s) 1416
BssAI RCCGGY 1 cut(s) 440
BssECI CCNNGG 7 cut(s) 174, 364, 505, 537, 644, 1277, 1503
BssT1I CCWWGG 5 cut(s) 174, 364, 505, 537, 1277
Bst2UI CCWGG 3 cut(s) 344, 1512, 1582
Bst4CI ACNGT 2 cut(s) 397, 919
Bst6I CTCTTC 2 cut(s) 531, 881
BstAFI CTTAAG 1 cut(s) 1399
BstAUI TGTACA 1 cut(s) 1416
BstBI TTCGAA 1 cut(s) 1217
BstDEI CTNAG 6 cut(s) 17, 152, 530, 1047, 1208, 1289
BstDSI CCRYGG 5 cut(s) 174, 505, 644, 1277, 1503
BstF5I GGATG 5 cut(s) 31, 445, 656, 733, 854
BstH2I RGCGCY 1 cut(s) 1440
BstHHI GCGC 2 cut(s) 1198, 1439
BstMAI GTCTC 4 cut(s) 20, 560, 636, 1659
BstMWI GCNNNNNNNGC 6 cut(s) 262, 704, 920, 1282, 1475, 1478
BstNI CCWGG 3 cut(s) 344, 1512, 1582
BstNSI RCATGY 1 cut(s) 832
BstSCI CCNGG 6 cut(s) 284, 342, 448, 474, 1510, 1580
BstSLI GKGCMC 2 cut(s) 9, 455
BstV1I GCAGC 5 cut(s) 416, 978, 1181, 1481, 1490
BstV2I GAAGAC 1 cut(s) 1674
BstX2I RGATCY 2 cut(s) 488, 501
BstXI CCANNNNNNTGG 1 cut(s) 1091
BstYI RGATCY 2 cut(s) 488, 501
BsuRI GGCC 3 cut(s) 315, 707, 1026
BtgI CCRYGG 5 cut(s) 174, 505, 644, 1277, 1503
BtrI CACGTC 2 cut(s) 570, 780
BtsCI GGATG 5 cut(s) 31, 445, 656, 733, 854
BtsI GCAGTG 1 cut(s) 891
BtsIMutI CAGTG 3 cut(s) 428, 891, 1044
BveI ACCTGC 1 cut(s) 1046
CfoI GCGC 2 cut(s) 1198, 1439
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 3 cut(s) 268, 870, 1346
Csp6I GTAC 2 cut(s) 416, 1417
CspAI ACCGGT 1 cut(s) 440
CspCI CAANNNNNGTGG 4 cut(s) 796, 831, 1147, 1182
CviAII CATG 9 cut(s) 125, 175, 506, 724, 829, 1003, 1278, 1317, 1405
CviQI GTAC 2 cut(s) 416, 1417
DdeI CTNAG 6 cut(s) 17, 152, 530, 1047, 1208, 1289
EaeI YGGCCR 1 cut(s) 705
Eam1104I CTCTTC 2 cut(s) 531, 881
EarI CTCTTC 2 cut(s) 531, 881
Eco130I CCWWGG 5 cut(s) 174, 364, 505, 537, 1277
Eco47I GGWCC 3 cut(s) 268, 870, 1346
Eco57I CTGAAG 2 cut(s) 1527, 1647
EcoRII CCWGG 3 cut(s) 342, 1510, 1580
EcoT14I CCWWGG 5 cut(s) 174, 364, 505, 537, 1277
ErhI CCWWGG 5 cut(s) 174, 364, 505, 537, 1277
Esp3I CGTCTC 1 cut(s) 560
FaeI CATG 9 cut(s) 128, 178, 509, 727, 832, 1006, 1281, 1320, 1408
FalI AAGNNNNNCTT 2 cut(s) 610, 642
FaqI GGGAC 3 cut(s) 186, 410, 856
FatI CATG 9 cut(s) 124, 174, 505, 723, 828, 1002, 1277, 1316, 1404
FauI CCCGC 1 cut(s) 979
FbaI TGATCA 2 cut(s) 967, 1563
FblI GTMKAC 1 cut(s) 1311
Fnu4HI GCNGC 5 cut(s) 405, 992, 1170, 1470, 1479
FokI GGATG 5 cut(s) 18, 432, 643, 740, 841
Fsp4HI GCNGC 5 cut(s) 405, 992, 1170, 1470, 1479
GlaI GCGC 2 cut(s) 1197, 1438
GluI GCNGC 5 cut(s) 405, 992, 1170, 1470, 1479
HaeII RGCGCY 1 cut(s) 1440
HaeIII GGCC 3 cut(s) 315, 707, 1026
HapII CCGG 5 cut(s) 266, 286, 441, 449, 476
HhaI GCGC 2 cut(s) 1198, 1439
Hin1II CATG 9 cut(s) 128, 178, 509, 727, 832, 1006, 1281, 1320, 1408
Hin6I GCGC 2 cut(s) 1196, 1437
HinP1I GCGC 2 cut(s) 1196, 1437
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 904
HinfI GANTC 1 cut(s) 732
HpaII CCGG 5 cut(s) 266, 286, 441, 449, 476
HphI GGTGA 5 cut(s) 160, 229, 430, 593, 1106
Hpy166II GTNNAC 5 cut(s) 361, 1224, 1312, 1387, 1419
Hpy188I TCNGA 5 cut(s) 75, 737, 1103, 1232, 1666
Hpy8I GTNNAC 5 cut(s) 361, 1224, 1312, 1387, 1419
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 5 cut(s) 605, 894, 1224, 1227, 1402
HpyCH4III ACNGT 2 cut(s) 397, 919
HpyCH4IV ACGT 2 cut(s) 569, 779
HpyCH4V TGCA 6 cut(s) 47, 896, 1169, 1268, 1522, 1688
HpyF10VI GCNNNNNNNGC 6 cut(s) 262, 704, 920, 1282, 1475, 1478
HpyF3I CTNAG 6 cut(s) 17, 152, 530, 1047, 1208, 1289
HpySE526I ACGT 2 cut(s) 569, 779
Hsp92II CATG 9 cut(s) 128, 178, 509, 727, 832, 1006, 1281, 1320, 1408
HspAI GCGC 2 cut(s) 1196, 1437
Ksp22I TGATCA 2 cut(s) 967, 1563
LmnI GCTCC 3 cut(s) 763, 1046, 1238
Lsp1109I GCAGC 5 cut(s) 416, 978, 1181, 1481, 1490
LweI GCATC 1 cut(s) 474
MaeII ACGT 2 cut(s) 569, 779
MaeIII GTNAC 2 cut(s) 1033, 1108
MboII GAAGA 6 cut(s) 518, 868, 991, 1533, 1588, 1679
MfeI CAATTG 1 cut(s) 1350
MflI RGATCY 2 cut(s) 488, 501
MhlI GDGCHC 3 cut(s) 9, 455, 514
MmeI TCCRAC 2 cut(s) 1108, 1306
MseI TTAA 2 cut(s) 101, 1400
MslI CAYNNNNRTG 1 cut(s) 173
MspCI CTTAAG 1 cut(s) 1399
MspI CCGG 5 cut(s) 266, 286, 441, 449, 476
MspR9I CCNGG 6 cut(s) 286, 344, 450, 476, 1512, 1582
MunI CAATTG 1 cut(s) 1350
Mva1269I GAATGC 1 cut(s) 1010
MvaI CCWGG 3 cut(s) 344, 1512, 1582
MwoI GCNNNNNNNGC 6 cut(s) 262, 704, 920, 1282, 1475, 1478
NciI CCSGG 3 cut(s) 286, 450, 476
NcoI CCATGG 3 cut(s) 174, 505, 1277
NlaIII CATG 9 cut(s) 128, 178, 509, 727, 832, 1006, 1281, 1320, 1408
NlaIV GGNNCC 6 cut(s) 6, 503, 872, 1185, 1347, 1509
NspI RCATGY 1 cut(s) 832
NspV TTCGAA 1 cut(s) 1217
PctI GAATGC 1 cut(s) 1010
PfeI GAWTC 1 cut(s) 732
PflMI CCANNNNNTGG 1 cut(s) 714
PinAI ACCGGT 1 cut(s) 440
PkrI GCNGC 5 cut(s) 406, 993, 1171, 1471, 1480
Psp6I CCWGG 3 cut(s) 342, 1510, 1580
PspGI CCWGG 3 cut(s) 342, 1510, 1580
PspN4I GGNNCC 6 cut(s) 6, 503, 872, 1185, 1347, 1509
PspPI GGNCC 3 cut(s) 268, 870, 1346
PsuI RGATCY 2 cut(s) 488, 501
RsaI GTAC 2 cut(s) 417, 1418
RsaNI GTAC 2 cut(s) 416, 1417
RseI CAYNNNNRTG 1 cut(s) 173
SaqAI TTAA 2 cut(s) 101, 1400
SatI GCNGC 5 cut(s) 405, 992, 1170, 1470, 1479
Sau96I GGNCC 3 cut(s) 268, 870, 1346
ScaI AGTACT 1 cut(s) 417
ScrFI CCNGG 6 cut(s) 286, 344, 450, 476, 1512, 1582
SduI GDGCHC 3 cut(s) 9, 455, 514
SfaNI GCATC 1 cut(s) 474
SfuI TTCGAA 1 cut(s) 1217
SinI GGWCC 3 cut(s) 268, 870, 1346
SmiMI CAYNNNNRTG 1 cut(s) 173
SmlI CTYRAG 3 cut(s) 251, 743, 1399
SmoI CTYRAG 3 cut(s) 251, 743, 1399
SpeI ACTAGT 1 cut(s) 421
SsiI CCGC 1 cut(s) 986
SspI AATATT 1 cut(s) 1119
StyD4I CCNGG 6 cut(s) 284, 342, 448, 474, 1510, 1580
StyI CCWWGG 5 cut(s) 174, 364, 505, 537, 1277
TaaI ACNGT 2 cut(s) 397, 919
TaiI ACGT 2 cut(s) 572, 782
TaqI TCGA 2 cut(s) 210, 1217
TaqII GACCGA 1 cut(s) 968
TatI WGTACW 2 cut(s) 415, 1416
TfiI GAWTC 1 cut(s) 732
Tru1I TTAA 2 cut(s) 101, 1400
Tru9I TTAA 2 cut(s) 101, 1400
TscAI CASTG 3 cut(s) 435, 898, 1051
TseI GCWGC 5 cut(s) 404, 991, 1169, 1469, 1478
TspDTI ATGAA 2 cut(s) 991, 1421
TspRI CASTG 3 cut(s) 435, 898, 1051
Van91I CCANNNNNTGG 1 cut(s) 714
Vha464I CTTAAG 1 cut(s) 1399
VpaK11BI GGWCC 3 cut(s) 268, 870, 1346
XapI RAATTY 1 cut(s) 206
XceI RCATGY 1 cut(s) 832
XmiI GTMKAC 1 cut(s) 1311
ZrmI AGTACT 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.