Rorug03G0227800

Glycogen synthase kinase-3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0019123
Physical Location & Seq
Forward (+)
20914214 .. 20915869
1656 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0227800.1

Sequence Viewer

Length: 645 bp
ATGTTCGACCCGAGAAAATCCTCAACATATAGGAACATTACTTGTTCTGCAAGGGAGTGTGGACTTGTCGATGATCAAAAACCACCAGGCCAATCACCGGAGTTTTGTAAAAAATTTCCTACAAGAAGGTGTACTTACCGTGTCGAGTATGAAGACACGTCATCTTCTGAAGGTGTATTGGCAAAAGAAACAATTGCCTTGACATCCAGGACAGGTAAGGTTGTAACCCTAAAAGATATTGTCATTGGGTGTGGACATTTGAACTATGAACATATTTCAACTGGAAATGAAATGGGAGTGATTGGGCTTGGAAATGGTCCCGTGTCATTTGTTTCTCAAATTGCTCCCTATGTTGGAGGTTACAGATTTTCTCATTGCTTGGTGCCTTTAGACACTGATCCCAAAATTGAAAGCAAGATCAATTTCGGAAATGGGAGTGAAGTTTTGGGTGAAGGGGTTGTCTCAACACCATTGGTTAATATAAGTATAGCCCCATCTAACTATTTTGTAACGGTAGAAGGAGTTACCATTGGAAATGAATTTGTTCCGTTCAACTCAACAGTGACACAGCTTAATAAACTTAACATGATGGTCGACTCGGGCTCAACTTTTGCACAAGTACATCGAGAATCGAGATATTTTTGA

Protein Analysis

214

Amino Acids

23.39

Weight (kDa)

6.1

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 1 - 144 5.8e-30 Xylanase inhibitor N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0019123)

Species Orthologous Gene IDs
rosa_rugosa Rorug03G0227800 Rorug03G0227900 Rorug03G0228000 Rorug03G0228000 Rorug03G0228100 Rorug03G0228200.1
rosa_samantha Rh3DG308200
rosa_wichuraiana Rw3G024650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 382
AccI GTMKAC 1 cut(s) 594
AclWI GGATC 1 cut(s) 392
AcsI RAATTY 2 cut(s) 113, 539
AcuI CTGAAG 1 cut(s) 189
AfaI GTAC 2 cut(s) 133, 621
AfiI CCNNNNNNNGG 2 cut(s) 97, 353
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 4 cut(s) 262, 279, 410, 553
AjiI CACGTC 1 cut(s) 159
AjnI CCWGG 2 cut(s) 85, 206
AluBI AGCT 1 cut(s) 571
AluI AGCT 1 cut(s) 571
Alw26I GTCTC 1 cut(s) 466
AlwI GGATC 1 cut(s) 392
Ama87I CYCGRG 2 cut(s) 10, 598
AoxI GGCC 1 cut(s) 88
ApoI RAATTY 2 cut(s) 113, 539
Asp700I GAANNNNTTC 1 cut(s) 543
AspS9I GGNCC 1 cut(s) 317
AsuHPI GGTGA 2 cut(s) 87, 461
AvaI CYCGRG 2 cut(s) 10, 598
AvaII GGWCC 1 cut(s) 317
BanI GGYRCC 1 cut(s) 382
BanII GRGCYC 1 cut(s) 605
BbsI GAAGAC 1 cut(s) 159
BccI CCATC 2 cut(s) 502, 583
BciT130I CCWGG 2 cut(s) 87, 208
BclI TGATCA 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 466
Bme1390I CCNGG 2 cut(s) 87, 208
Bme18I GGWCC 1 cut(s) 317
BmeT110I CYCGRG 2 cut(s) 10, 598
BmgBI CACGTC 1 cut(s) 159
BmgT120I GGNCC 1 cut(s) 317
BmiI GGNNCC 2 cut(s) 319, 384
BmrFI CCNGG 2 cut(s) 87, 208
BpiI GAAGAC 1 cut(s) 159
BsaWI WCCGGW 1 cut(s) 97
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 353
Bse1I ACTGG 1 cut(s) 286
Bse3DI GCAATG 1 cut(s) 373
BseBI CCWGG 2 cut(s) 87, 208
BseGI GGATG 1 cut(s) 203
BseLI CCNNNNNNNGG 2 cut(s) 97, 353
BseMI GCAATG 1 cut(s) 373
BseNI ACTGG 1 cut(s) 286
BshFI GGCC 1 cut(s) 90
BshNI GGYRCC 1 cut(s) 382
BsiHKCI CYCGRG 2 cut(s) 10, 598
BsiSI CCGG 1 cut(s) 98
BslFI GGGAC 1 cut(s) 303
BslI CCNNNNNNNGG 2 cut(s) 97, 353
BsmAI GTCTC 1 cut(s) 466
BsmFI GGGAC 1 cut(s) 303
BsnI GGCC 1 cut(s) 90
BsoBI CYCGRG 2 cut(s) 10, 598
Bsp1286I GDGCHC 1 cut(s) 605
Bsp143I GATC 3 cut(s) 73, 397, 417
BspANI GGCC 1 cut(s) 90
BspLI GGNNCC 2 cut(s) 319, 384
BspPI GGATC 1 cut(s) 392
BspT107I GGYRCC 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 373
BsrI ACTGG 1 cut(s) 286
BssMI GATC 3 cut(s) 73, 397, 417
Bst2UI CCWGG 2 cut(s) 87, 208
Bst4CI ACNGT 3 cut(s) 140, 514, 562
BstF5I GGATG 1 cut(s) 203
BstKTI GATC 3 cut(s) 76, 400, 420
BstMAI GTCTC 1 cut(s) 466
BstMBI GATC 3 cut(s) 73, 397, 417
BstNI CCWGG 2 cut(s) 87, 208
BstSCI CCNGG 2 cut(s) 85, 206
BstV2I GAAGAC 1 cut(s) 159
BsuRI GGCC 1 cut(s) 90
BtrI CACGTC 1 cut(s) 159
BtsCI GGATG 1 cut(s) 203
BtsIMutI CAGTG 2 cut(s) 393, 567
Cfr13I GGNCC 1 cut(s) 317
Csp6I GTAC 2 cut(s) 132, 620
CviAII CATG 1 cut(s) 586
CviJI RGCY 5 cut(s) 90, 307, 491, 571, 603
CviKI_1 RGCY 5 cut(s) 90, 307, 491, 571, 603
CviQI GTAC 2 cut(s) 132, 620
DpnI GATC 3 cut(s) 75, 399, 419
DpnII GATC 3 cut(s) 73, 397, 417
Eco24I GRGCYC 1 cut(s) 605
Eco47I GGWCC 1 cut(s) 317
Eco57I CTGAAG 1 cut(s) 189
Eco88I CYCGRG 2 cut(s) 10, 598
EcoRII CCWGG 2 cut(s) 85, 206
EcoT38I GRGCYC 1 cut(s) 605
FaeI CATG 1 cut(s) 589
FaiI YATR 9 cut(s) 28, 30, 150, 267, 273, 351, 482, 488, 587
FalI AAGNNNNNCTT 2 cut(s) 118, 150
FaqI GGGAC 1 cut(s) 303
FatI CATG 1 cut(s) 585
FbaI TGATCA 1 cut(s) 73
FblI GTMKAC 1 cut(s) 594
FokI GGATG 1 cut(s) 190
FriOI GRGCYC 1 cut(s) 605
HaeIII GGCC 1 cut(s) 90
HapII CCGG 1 cut(s) 98
Hin1II CATG 1 cut(s) 589
HincII GTYRAC 1 cut(s) 595
HindII GTYRAC 1 cut(s) 595
HinfI GANTC 2 cut(s) 596, 629
HpaII CCGG 1 cut(s) 98
HphI GGTGA 2 cut(s) 87, 461
Hpy166II GTNNAC 4 cut(s) 62, 132, 254, 595
Hpy188I TCNGA 2 cut(s) 169, 428
Hpy188III TCNNGA 2 cut(s) 626, 633
Hpy8I GTNNAC 4 cut(s) 62, 132, 254, 595
HpyAV CCTTC 4 cut(s) 120, 164, 446, 512
HpyCH4III ACNGT 3 cut(s) 140, 514, 562
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 2 cut(s) 50, 614
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 1 cut(s) 589
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 3 cut(s) 73, 397, 417
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 7 cut(s) 72, 99, 111, 193, 198, 220, 267
MaeII ACGT 1 cut(s) 158
MaeIII GTNAC 5 cut(s) 223, 359, 508, 523, 562
MalI GATC 3 cut(s) 75, 399, 419
MboI GATC 3 cut(s) 73, 397, 417
MboII GAAGA 2 cut(s) 156, 164
MfeI CAATTG 1 cut(s) 192
MhlI GDGCHC 1 cut(s) 605
MluCI AATT 6 cut(s) 113, 192, 339, 405, 421, 539
MlyI GAGTC 1 cut(s) 590
MmeI TCCRAC 1 cut(s) 334
MnlI CCTC 2 cut(s) 31, 350
MroXI GAANNNNTTC 1 cut(s) 543
MseI TTAA 3 cut(s) 477, 573, 582
MspI CCGG 1 cut(s) 98
MspR9I CCNGG 2 cut(s) 87, 208
MunI CAATTG 1 cut(s) 192
MvaI CCWGG 2 cut(s) 87, 208
NdeII GATC 3 cut(s) 73, 397, 417
NlaIII CATG 1 cut(s) 589
NlaIV GGNNCC 2 cut(s) 319, 384
NmuCI GTSAC 1 cut(s) 562
PdmI GAANNNNTTC 1 cut(s) 543
PfeI GAWTC 1 cut(s) 629
PfoI TCCNGGA 1 cut(s) 206
PleI GAGTC 1 cut(s) 590
PpsI GAGTC 1 cut(s) 590
Psp6I CCWGG 2 cut(s) 85, 206
PspGI CCWGG 2 cut(s) 85, 206
PspN4I GGNNCC 2 cut(s) 319, 384
PspPI GGNCC 1 cut(s) 317
RsaI GTAC 2 cut(s) 133, 621
RsaNI GTAC 2 cut(s) 132, 620
SalI GTCGAC 1 cut(s) 593
SaqAI TTAA 3 cut(s) 477, 573, 582
Sau3AI GATC 3 cut(s) 73, 397, 417
Sau96I GGNCC 1 cut(s) 317
SchI GAGTC 1 cut(s) 590
ScrFI CCNGG 2 cut(s) 87, 208
SduI GDGCHC 1 cut(s) 605
SetI ASST 7 cut(s) 131, 161, 175, 217, 222, 361, 573
SinI GGWCC 1 cut(s) 317
Sse9I AATT 6 cut(s) 113, 192, 339, 405, 421, 539
StyD4I CCNGG 2 cut(s) 85, 206
TaaI ACNGT 3 cut(s) 140, 514, 562
TaiI ACGT 1 cut(s) 161
TaqI TCGA 6 cut(s) 6, 69, 144, 594, 625, 632
TasI AATT 6 cut(s) 113, 192, 339, 405, 421, 539
TatI WGTACW 2 cut(s) 131, 619
TfiI GAWTC 1 cut(s) 629
Tru1I TTAA 3 cut(s) 477, 573, 582
Tru9I TTAA 3 cut(s) 477, 573, 582
TscAI CASTG 2 cut(s) 400, 567
TseFI GTSAC 1 cut(s) 562
Tsp45I GTSAC 1 cut(s) 562
TspDTI ATGAA 4 cut(s) 165, 282, 303, 552
TspGWI ACGGA 1 cut(s) 537
TspRI CASTG 2 cut(s) 400, 567
VpaK11BI GGWCC 1 cut(s) 317
XapI RAATTY 2 cut(s) 113, 539
XmiI GTMKAC 1 cut(s) 594
XmnI GAANNNNTTC 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.