Rorug03G0228000

Glycogen synthase kinase-3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0019123
Physical Location & Seq
Forward (+)
20927350 .. 20929506
2157 bp
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UTR
Exon/CDS
Intron
Rorug03G0228000.1

Sequence Viewer

Length: 627 bp
ATGGCAACTGAAGTTGTAGCCGAGCCAATCCAGCATTTCCAGGAACCTCACAACGACGTCAAGCTCTTCAACAAGTGGAGCTTCGACGATGTTCAGGTCAGTGACATCTCACTTGGAGATTACATTGGAGTGGCCCAGTCCAAACATGCAACATATGTGCCTCACACTGCTGGGAGGTACTCAGTGAAACGTTTCAGGAAAGCTCAATGCCCCATTGTGGAGAGGCTTACAAACTCACTCATGATGCACGGGAGAAACAACGGGAAGAAGTTGATGGCTGTTAGGATTGTTAGGCATGCAATGGAAATCATCCATTTGCTTACTGATCTGAACCCCATTCAAGTTATTGTTGATGCTGTTGTTAACAGTGGTCCACGTGAAGATGCTACTCGTATTGGGTCTGCCGGAGTTGTTAGGCGTCAGGCTGTGGATATCTCACCTCTGAGACGGGTGAACCAGGCTATCTATCTCATCACAACTGGTGCTCGTGAGTCTGCTTTCAGGAACATCAAAACCATTGCTGAATGCTTGGCCGATGAGCTTATTAATGCTGCCAAAGGTTCCTCTAACAGTTATGCTATCAAGAAGAAGGATGAGATTGAAAGGGTTGCCAAGGCCAATCGTTAA

Protein Analysis

208

Amino Acids

23.21

Weight (kDa)

9.62

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S7 PF00177 48 - 208 1e-41 Ribosomal protein S7p/S5e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0019123)

Species Orthologous Gene IDs
rosa_rugosa Rorug03G0227800 Rorug03G0227900 Rorug03G0228000 Rorug03G0228000 Rorug03G0228100 Rorug03G0228200.1
rosa_samantha Rh3DG308200
rosa_wichuraiana Rw3G024650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 60
AclI AACGTT 1 cut(s) 190
AcoI YGGCCR 1 cut(s) 531
AcuI CTGAAG 1 cut(s) 30
AcvI CACGTG 1 cut(s) 377
AcyI GRCGYC 2 cut(s) 57, 418
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 3 cut(s) 70, 341, 602
AjnI CCWGG 2 cut(s) 39, 456
AluBI AGCT 4 cut(s) 64, 81, 203, 541
AluI AGCT 4 cut(s) 64, 81, 203, 541
Alw21I GWGCWC 1 cut(s) 487
Alw26I GTCTC 1 cut(s) 439
AoxI GGCC 3 cut(s) 132, 531, 615
ApeKI GCWGC 1 cut(s) 551
AseI ATTAAT 1 cut(s) 546
Asp700I GAANNNNTTC 1 cut(s) 191
AspS9I GGNCC 2 cut(s) 133, 371
AsuHPI GGTGA 2 cut(s) 429, 463
AvaII GGWCC 1 cut(s) 371
BauI CACGAG 1 cut(s) 486
BbrPI CACGTG 1 cut(s) 377
Bbv12I GWGCWC 1 cut(s) 487
BbvI GCAGC 1 cut(s) 538
BccI CCATC 1 cut(s) 268
BciT130I CCWGG 2 cut(s) 41, 458
BcoDI GTCTC 1 cut(s) 439
BisI GCNGC 1 cut(s) 552
BlsI GCNGC 1 cut(s) 553
Bme1390I CCNGG 2 cut(s) 41, 458
Bme18I GGWCC 1 cut(s) 371
BmgT120I GGNCC 2 cut(s) 133, 371
BmiI GGNNCC 2 cut(s) 45, 562
BmrFI CCNGG 2 cut(s) 41, 458
BmrI ACTGGG 1 cut(s) 130
BmsI GCATC 3 cut(s) 234, 343, 373
BmuI ACTGGG 1 cut(s) 130
BsaAI YACGTR 1 cut(s) 377
BsaHI GRCGYC 2 cut(s) 57, 418
BsaJI CCNNGG 1 cut(s) 612
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse1I ACTGG 2 cut(s) 136, 484
Bse3DI GCAATG 2 cut(s) 306, 516
BseBI CCWGG 2 cut(s) 41, 458
BseDI CCNNGG 1 cut(s) 612
BseGI GGATG 2 cut(s) 309, 598
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 2 cut(s) 306, 516
BseMII CTCAG 2 cut(s) 195, 434
BseNI ACTGG 2 cut(s) 136, 484
BseXI GCAGC 1 cut(s) 538
BseYI CCCAGC 1 cut(s) 170
BshFI GGCC 3 cut(s) 134, 533, 617
BsiHKAI GWGCWC 1 cut(s) 487
BsiSI CCGG 1 cut(s) 405
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 1 cut(s) 439
BsmBI CGTCTC 1 cut(s) 439
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 3 cut(s) 134, 533, 617
Bsp1286I GDGCHC 1 cut(s) 487
Bsp143I GATC 1 cut(s) 325
BspANI GGCC 3 cut(s) 134, 533, 617
BspCNI CTCAG 2 cut(s) 194, 435
BspHI TCATGA 1 cut(s) 240
BspLI GGNNCC 2 cut(s) 45, 562
BspQI GCTCTTC 1 cut(s) 71
BsrDI GCAATG 2 cut(s) 306, 516
BsrI ACTGG 2 cut(s) 136, 484
BssECI CCNNGG 1 cut(s) 612
BssMI GATC 1 cut(s) 325
BssNI GRCGYC 2 cut(s) 57, 418
BssSI CACGAG 1 cut(s) 486
BssT1I CCWWGG 1 cut(s) 612
Bst2BI CACGAG 1 cut(s) 486
Bst2UI CCWGG 2 cut(s) 41, 458
Bst4CI ACNGT 2 cut(s) 368, 572
Bst6I CTCTTC 1 cut(s) 71
BstACI GRCGYC 2 cut(s) 57, 418
BstBAI YACGTR 1 cut(s) 377
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 2 cut(s) 181, 443
BstF5I GGATG 2 cut(s) 309, 598
BstKTI GATC 1 cut(s) 328
BstMAI GTCTC 1 cut(s) 439
BstMBI GATC 1 cut(s) 325
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstNI CCWGG 2 cut(s) 41, 458
BstNSI RCATGY 2 cut(s) 149, 299
BstSCI CCNGG 2 cut(s) 39, 456
BstV1I GCAGC 1 cut(s) 538
BsuRI GGCC 3 cut(s) 134, 533, 617
BtsCI GGATG 2 cut(s) 309, 598
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 4 cut(s) 106, 165, 189, 373
Cac8I GCNNGC 1 cut(s) 297
CciI TCATGA 1 cut(s) 240
Cfr13I GGNCC 2 cut(s) 133, 371
CseI GACGC 1 cut(s) 407
Csp6I GTAC 1 cut(s) 178
CviAII CATG 3 cut(s) 146, 241, 296
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 2 cut(s) 181, 443
DpnI GATC 1 cut(s) 327
DpnII GATC 1 cut(s) 325
EaeI YGGCCR 1 cut(s) 531
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 612
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 30
Eco72I CACGTG 1 cut(s) 377
EcoRII CCWGG 2 cut(s) 39, 456
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 612
ErhI CCWWGG 1 cut(s) 612
Esp3I CGTCTC 1 cut(s) 439
FaeI CATG 3 cut(s) 149, 244, 299
FaiI YATR 6 cut(s) 147, 154, 156, 242, 297, 576
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FatI CATG 3 cut(s) 145, 240, 295
FauNDI CATATG 1 cut(s) 154
Fnu4HI GCNGC 1 cut(s) 552
FokI GGATG 2 cut(s) 296, 605
Fsp4HI GCNGC 1 cut(s) 552
GluI GCNGC 1 cut(s) 552
GsaI CCCAGC 1 cut(s) 174
HaeIII GGCC 3 cut(s) 134, 533, 617
HapII CCGG 1 cut(s) 405
HgaI GACGC 1 cut(s) 407
Hin1I GRCGYC 2 cut(s) 57, 418
Hin1II CATG 3 cut(s) 149, 244, 299
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 1 cut(s) 491
HpaI GTTAAC 1 cut(s) 364
HpaII CCGG 1 cut(s) 405
HphI GGTGA 2 cut(s) 429, 463
Hpy166II GTNNAC 3 cut(s) 364, 374, 454
Hpy188I TCNGA 2 cut(s) 330, 444
Hpy188III TCNNGA 5 cut(s) 196, 241, 488, 502, 583
Hpy8I GTNNAC 3 cut(s) 364, 374, 454
Hpy99I CGWCG 2 cut(s) 59, 89
HpyAV CCTTC 1 cut(s) 583
HpyCH4III ACNGT 2 cut(s) 368, 572
HpyCH4IV ACGT 3 cut(s) 57, 190, 376
HpyCH4V TGCA 3 cut(s) 149, 247, 299
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 2 cut(s) 181, 443
HpySE526I ACGT 3 cut(s) 57, 190, 376
Hsp92I GRCGYC 2 cut(s) 57, 418
Hsp92II CATG 3 cut(s) 149, 244, 299
KspAI GTTAAC 1 cut(s) 364
Kzo9I GATC 1 cut(s) 325
LguI GCTCTTC 1 cut(s) 71
LmnI GCTCC 1 cut(s) 78
Lsp1109I GCAGC 1 cut(s) 538
LweI GCATC 3 cut(s) 234, 343, 373
MaeII ACGT 3 cut(s) 57, 190, 376
MaeIII GTNAC 1 cut(s) 101
MalI GATC 1 cut(s) 327
MboI GATC 1 cut(s) 325
MboII GAAGA 4 cut(s) 58, 277, 392, 598
MhlI GDGCHC 1 cut(s) 487
MlyI GAGTC 1 cut(s) 500
MnlI CCTC 6 cut(s) 57, 168, 171, 216, 450, 574
MroXI GAANNNNTTC 1 cut(s) 191
MseI TTAA 3 cut(s) 363, 546, 625
MslI CAYNNNNRTG 1 cut(s) 128
MspI CCGG 1 cut(s) 405
MspR9I CCNGG 2 cut(s) 41, 458
Mva1269I GAATGC 1 cut(s) 530
MvaI CCWGG 2 cut(s) 41, 458
MwoI GCNNNNNNNGC 1 cut(s) 31
NdeI CATATG 1 cut(s) 154
NdeII GATC 1 cut(s) 325
NlaIII CATG 3 cut(s) 149, 244, 299
NlaIV GGNNCC 2 cut(s) 45, 562
NmeAIII GCCGAG 1 cut(s) 46
NmuCI GTSAC 1 cut(s) 101
NspI RCATGY 2 cut(s) 149, 299
PaeI GCATGC 1 cut(s) 299
PagI TCATGA 1 cut(s) 240
PciSI GCTCTTC 1 cut(s) 71
PctI GAATGC 1 cut(s) 530
PdmI GAANNNNTTC 1 cut(s) 191
PfoI TCCNGGA 1 cut(s) 39
PkrI GCNGC 1 cut(s) 553
PleI GAGTC 1 cut(s) 499
PmaCI CACGTG 1 cut(s) 377
PmlI CACGTG 1 cut(s) 377
PpsI GAGTC 1 cut(s) 499
Ppu21I YACGTR 1 cut(s) 377
PshBI ATTAAT 1 cut(s) 546
Psp1406I AACGTT 1 cut(s) 190
Psp6I CCWGG 2 cut(s) 39, 456
PspCI CACGTG 1 cut(s) 377
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 2 cut(s) 39, 456
PspN4I GGNNCC 2 cut(s) 45, 562
PspPI GGNCC 2 cut(s) 133, 371
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
RseI CAYNNNNRTG 1 cut(s) 128
SapI GCTCTTC 1 cut(s) 71
SaqAI TTAA 3 cut(s) 363, 546, 625
SatI GCNGC 1 cut(s) 552
Sau3AI GATC 1 cut(s) 325
Sau96I GGNCC 2 cut(s) 133, 371
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 2 cut(s) 41, 458
SduI GDGCHC 1 cut(s) 487
SfaNI GCATC 3 cut(s) 234, 343, 373
SinI GGWCC 1 cut(s) 371
SmiMI CAYNNNNRTG 1 cut(s) 128
SphI GCATGC 1 cut(s) 299
StyD4I CCNGG 2 cut(s) 39, 456
StyI CCWWGG 1 cut(s) 612
TaaI ACNGT 2 cut(s) 368, 572
TaiI ACGT 3 cut(s) 60, 193, 379
TaqI TCGA 1 cut(s) 84
Tru1I TTAA 3 cut(s) 363, 546, 625
Tru9I TTAA 3 cut(s) 363, 546, 625
TscAI CASTG 4 cut(s) 106, 172, 189, 373
TseFI GTSAC 1 cut(s) 101
TseI GCWGC 1 cut(s) 551
Tsp45I GTSAC 1 cut(s) 101
TspRI CASTG 4 cut(s) 106, 172, 189, 373
VpaK11BI GGWCC 1 cut(s) 371
VspI ATTAAT 1 cut(s) 546
XceI RCATGY 2 cut(s) 149, 299
XmnI GAANNNNTTC 1 cut(s) 191
ZraI GACGTC 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.