Rorug04G0213100

YT521-B-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005237
Physical Location & Seq
Forward (+)
37004590 .. 37014984
10395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0213100.1

Sequence Viewer

Length: 1347 bp
ATGATGAGTCGCACTCTTCGTTCCAAGACGCTTCCTTTGCTCTTCCGCAACCCCAAAGTGAACCCATGTGAACTCTGGGCAGTTCCATTCCAAGCCCTTTTCTCCACACACGTTGTTGGTGAAAAACCAGTTCTCGTTCGTGATTTCATTCATTCTGCACTATATGACCCTCGACATGGTTATTTCGCTCAAAGATCGAGGTCGGTTGGTGTGCTTCAGAAAGCCATTAAGTTCAATCAGATTGAGGGCAGGAAAGCTTACATGAAATACTTGGATAAAATATACAAGGGAAGTGAGATATCGTGGTTTACTCCTGTGGAACTTTTTAAGCCTTGGTATGCCCACGGGATAGCTGAAGCCATAATGCGTACTGCTAACCTTTCAGTTCCCCTCAAGATATATGAAATTGGTGGTGGATCAGGAACTTGTGCAAAGGGAATCATGGATTACATATTGTTGAATGCACCTACCAGAGTTTACAACAATATGACTTACGTCTCAGTTGAAATCAGTCCCTCACTGGCTGAGCTACAAAGAGAAACTGTTGGAGAAGTTCGTAGTCACTTATCAAAGTTTCGAGTCGAATGCCGTGATGCTGCTGACAGGAAGGGATGGGGGGATGTGGAGCAAGAGCCATGCTGGGTAATAATGCTCGAGGTACTTGATAATCTTCCACATGATCTCATCTATTCTGAAAATCAAGTTTCACCATGGAAGGAAGTTTGGGTGGAGAACCAAGATGGAGAAACACTATCTGAATTGTACAAGCCATTGCAAGACTCACTAATTAAGCGATGCATTGAGATCATCGACTTCGAGAAAAAACATGATTCTCAAAGCAGTGCATTATTGAAAGCAAAAAGTGATTGGTCTAAAGTCTTCCCAAAACCTCGAAGAAGTTGGCTACCAACTGGTTGCTTGAAACTACTTGAAGTGTTACATGGAGCACTACCAAAGATGTCTTTGATTGCTTCAGACTTTAGTTACCTACCTGACGTGAGAATACTTGGTGAAAGAGCTCCATTGGTTTCTACCAAGAAAGATGGCAGCAGCTCAGACTATGGAAGTTATCTTGATGCAAAGGGGGATGCTGATATCTTCTTCCCCACTGAATTTTGGTTATTGGAACGTATTGATCATTACTGTTCTGGATGGATGAAGCTGCAAAAAGATAACTCATCCAAAGAAGGAAAGAAGAGGCGAACAATTATACTTGATACATCTTCATTCATGGAAGAATTTGGATTGCCCTCGAGGACAAGAACCAAGGATGGATACAATCCACTTTTGGATGACTTCAAGAATACAAAATTCTATCTAAGTGTCCCAACACATAATACTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YTH

Protein Analysis

448

Amino Acids

51.3

Weight (kDa)

8.6

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_28 PF02636 121 - 385 7.1e-31 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1252
AciI CCGC 1 cut(s) 46
AclWI GGATC 1 cut(s) 424
AcsI RAATTY 3 cut(s) 1112, 1238, 1310
AcuI CTGAAG 3 cut(s) 200, 375, 957
AfaI GTAC 3 cut(s) 370, 660, 764
AfiI CCNNNNNNNGG 1 cut(s) 176
AflIII ACRYGT 1 cut(s) 109
AgsI TTSAA 7 cut(s) 235, 460, 506, 853, 922, 932, 1300
AjiI CACGTC 1 cut(s) 997
AluBI AGCT 6 cut(s) 257, 353, 529, 1019, 1053, 1162
AluI AGCT 6 cut(s) 257, 353, 529, 1019, 1053, 1162
Alw21I GWGCWC 2 cut(s) 949, 1021
Alw26I GTCTC 1 cut(s) 502
AlwI GGATC 1 cut(s) 424
Ama87I CYCGRG 2 cut(s) 653, 1252
ApeKI GCWGC 4 cut(s) 596, 1047, 1050, 1162
ApoI RAATTY 3 cut(s) 1112, 1238, 1310
ArsI GACNNNNNNTTYG 2 cut(s) 19, 51
AsuHPI GGTGA 3 cut(s) 131, 699, 1022
AvaI CYCGRG 2 cut(s) 653, 1252
BanII GRGCYC 1 cut(s) 1021
BbsI GAAGAC 1 cut(s) 871
Bbv12I GWGCWC 2 cut(s) 949, 1021
BbvI GCAGC 4 cut(s) 583, 1059, 1062, 1149
BccI CCATC 5 cut(s) 606, 734, 1037, 1146, 1265
BceAI ACGGC 1 cut(s) 573
BcgI CGANNNNNNTGC 2 cut(s) 567, 601
BciVI GTATCC 1 cut(s) 1268
BclI TGATCA 1 cut(s) 1135
BcoDI GTCTC 1 cut(s) 502
BfuI GTATCC 1 cut(s) 1268
BisI GCNGC 4 cut(s) 597, 1048, 1051, 1163
BlpI GCTNAGC 1 cut(s) 525
BlsI GCNGC 4 cut(s) 598, 1049, 1052, 1164
BmeT110I CYCGRG 2 cut(s) 653, 1252
BmgBI CACGTC 1 cut(s) 997
BmsI GCATC 4 cut(s) 583, 785, 1066, 1078
BoxI GACNNNNGTC 1 cut(s) 494
BpiI GAAGAC 1 cut(s) 871
BplI GAGNNNNNCTC 1 cut(s) 30
Bpu1102I GCTNAGC 1 cut(s) 525
BpuEI CTTGAG 1 cut(s) 377
BsaJI CCNNGG 4 cut(s) 332, 343, 710, 1266
Bsc4I CCNNNNNNNGG 1 cut(s) 176
Bse1I ACTGG 3 cut(s) 128, 525, 916
Bse3DI GCAATG 1 cut(s) 770
BseDI CCNNGG 4 cut(s) 332, 343, 710, 1266
BseGI GGATG 8 cut(s) 617, 625, 1093, 1157, 1161, 1178, 1276, 1297
BseLI CCNNNNNNNGG 1 cut(s) 176
BseMI GCAATG 1 cut(s) 770
BseMII CTCAG 3 cut(s) 513, 516, 1068
BseNI ACTGG 3 cut(s) 128, 525, 916
BseXI GCAGC 4 cut(s) 583, 1059, 1062, 1149
BseYI CCCAGC 1 cut(s) 639
BsgI GTGCAG 1 cut(s) 141
BsiHKAI GWGCWC 2 cut(s) 949, 1021
BsiHKCI CYCGRG 2 cut(s) 653, 1252
BslFI GGGAC 2 cut(s) 498, 1310
BslI CCNNNNNNNGG 1 cut(s) 176
BsmAI GTCTC 1 cut(s) 502
BsmBI CGTCTC 1 cut(s) 502
BsmFI GGGAC 2 cut(s) 498, 1310
BsmI GAATGC 2 cut(s) 466, 590
BsoBI CYCGRG 2 cut(s) 653, 1252
Bsp1286I GDGCHC 2 cut(s) 949, 1021
Bsp1407I TGTACA 1 cut(s) 762
Bsp143I GATC 5 cut(s) 194, 416, 679, 804, 1135
Bsp1720I GCTNAGC 1 cut(s) 525
Bsp19I CCATGG 1 cut(s) 710
BspACI CCGC 1 cut(s) 46
BspCNI CTCAG 3 cut(s) 512, 517, 1067
BspPI GGATC 1 cut(s) 424
BspQI GCTCTTC 1 cut(s) 47
BsrDI GCAATG 1 cut(s) 770
BsrGI TGTACA 1 cut(s) 762
BsrI ACTGG 3 cut(s) 128, 525, 916
BssECI CCNNGG 4 cut(s) 332, 343, 710, 1266
BssMI GATC 5 cut(s) 194, 416, 679, 804, 1135
BssT1I CCWWGG 3 cut(s) 332, 710, 1266
Bst4CI ACNGT 2 cut(s) 544, 1145
Bst6I CTCTTC 3 cut(s) 21, 47, 1190
BstAUI TGTACA 1 cut(s) 762
BstDEI CTNAG 4 cut(s) 499, 525, 1054, 1319
BstDSI CCRYGG 2 cut(s) 343, 710
BstF5I GGATG 8 cut(s) 617, 625, 1093, 1157, 1161, 1178, 1276, 1297
BstKTI GATC 5 cut(s) 197, 419, 682, 807, 1138
BstMAI GTCTC 1 cut(s) 502
BstMBI GATC 5 cut(s) 194, 416, 679, 804, 1135
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstPAI GACNNNNGTC 1 cut(s) 494
BstV1I GCAGC 4 cut(s) 583, 1059, 1062, 1149
BstV2I GAAGAC 1 cut(s) 871
BsuI GTATCC 1 cut(s) 1268
BtgI CCRYGG 2 cut(s) 343, 710
BtgZI GCGATG 1 cut(s) 808
BtrI CACGTC 1 cut(s) 997
BtsCI GGATG 8 cut(s) 617, 625, 1093, 1157, 1161, 1178, 1276, 1297
BtsI GCAGTG 1 cut(s) 847
BtsIMutI CAGTG 3 cut(s) 518, 847, 1107
CseI GACGC 1 cut(s) 37
Csp6I GTAC 3 cut(s) 369, 659, 763
CspCI CAANNNNNGTGG 2 cut(s) 94, 129
CviQI GTAC 3 cut(s) 369, 659, 763
DdeI CTNAG 4 cut(s) 499, 525, 1054, 1319
DpnI GATC 5 cut(s) 196, 418, 681, 806, 1137
DpnII GATC 5 cut(s) 194, 416, 679, 804, 1135
Eam1104I CTCTTC 3 cut(s) 21, 47, 1190
EarI CTCTTC 3 cut(s) 21, 47, 1190
Ecl136II GAGCTC 1 cut(s) 1019
Eco130I CCWWGG 3 cut(s) 332, 710, 1266
Eco24I GRGCYC 1 cut(s) 1021
Eco32I GATATC 2 cut(s) 300, 1096
Eco53kI GAGCTC 1 cut(s) 1019
Eco57I CTGAAG 3 cut(s) 200, 375, 957
Eco88I CYCGRG 2 cut(s) 653, 1252
EcoICRI GAGCTC 1 cut(s) 1019
EcoRV GATATC 2 cut(s) 300, 1096
EcoT14I CCWWGG 3 cut(s) 332, 710, 1266
EcoT22I ATGCAT 1 cut(s) 800
EcoT38I GRGCYC 1 cut(s) 1021
ErhI CCWWGG 3 cut(s) 332, 710, 1266
Esp3I CGTCTC 1 cut(s) 502
FaqI GGGAC 2 cut(s) 498, 1310
FbaI TGATCA 1 cut(s) 1135
Fnu4HI GCNGC 4 cut(s) 597, 1048, 1051, 1163
FokI GGATG 8 cut(s) 624, 632, 1100, 1164, 1165, 1168, 1283, 1304
FriOI GRGCYC 1 cut(s) 1021
Fsp4HI GCNGC 4 cut(s) 597, 1048, 1051, 1163
GluI GCNGC 4 cut(s) 597, 1048, 1051, 1163
GsaI CCCAGC 1 cut(s) 643
HgaI GACGC 1 cut(s) 37
HindIII AAGCTT 1 cut(s) 255
HinfI GANTC 5 cut(s) 7, 438, 579, 779, 830
HphI GGTGA 3 cut(s) 131, 699, 1022
Hpy166II GTNNAC 4 cut(s) 61, 71, 309, 478
Hpy188I TCNGA 6 cut(s) 219, 240, 694, 757, 976, 1057
Hpy188III TCNNGA 7 cut(s) 140, 394, 420, 817, 1073, 1149, 1300
Hpy8I GTNNAC 4 cut(s) 61, 71, 309, 478
HpyAV CCTTC 3 cut(s) 601, 709, 1181
HpyCH4III ACNGT 2 cut(s) 544, 1145
HpyCH4IV ACGT 4 cut(s) 111, 495, 996, 1129
HpyCH4V TGCA 8 cut(s) 158, 431, 464, 775, 798, 845, 1079, 1165
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 4 cut(s) 499, 525, 1054, 1319
HpySE526I ACGT 4 cut(s) 111, 495, 996, 1129
Ksp22I TGATCA 1 cut(s) 1135
Kzo9I GATC 5 cut(s) 194, 416, 679, 804, 1135
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 3 cut(s) 625, 944, 1024
Lsp1109I GCAGC 4 cut(s) 583, 1059, 1062, 1149
LweI GCATC 4 cut(s) 583, 785, 1066, 1078
MaeII ACGT 4 cut(s) 111, 495, 996, 1129
MaeIII GTNAC 3 cut(s) 560, 936, 983
MalI GATC 5 cut(s) 196, 418, 681, 806, 1137
MboI GATC 5 cut(s) 194, 416, 679, 804, 1135
MhlI GDGCHC 2 cut(s) 949, 1021
MluCI AATT 7 cut(s) 405, 758, 786, 1112, 1206, 1238, 1310
MlyI GAGTC 3 cut(s) 16, 588, 773
MmeI TCCRAC 1 cut(s) 526
Mph1103I ATGCAT 1 cut(s) 800
MseI TTAA 3 cut(s) 228, 327, 789
Mva1269I GAATGC 2 cut(s) 466, 590
MwoI GCNNNNNNNGC 1 cut(s) 37
NcoI CCATGG 1 cut(s) 710
NdeII GATC 5 cut(s) 194, 416, 679, 804, 1135
NmuCI GTSAC 1 cut(s) 560
NsiI ATGCAT 1 cut(s) 800
PaeR7I CTCGAG 2 cut(s) 653, 1252
PciSI GCTCTTC 1 cut(s) 47
PcsI WCGNNNNNNNCGW 1 cut(s) 16
PctI GAATGC 2 cut(s) 466, 590
PfeI GAWTC 2 cut(s) 438, 830
PkrI GCNGC 4 cut(s) 598, 1049, 1052, 1164
PleI GAGTC 3 cut(s) 15, 587, 773
PpsI GAGTC 3 cut(s) 15, 587, 773
PshAI GACNNNNGTC 1 cut(s) 494
Psp124BI GAGCTC 1 cut(s) 1021
PspFI CCCAGC 1 cut(s) 639
PspXI VCTCGAGB 2 cut(s) 653, 1252
RsaI GTAC 3 cut(s) 370, 660, 764
RsaNI GTAC 3 cut(s) 369, 659, 763
SacI GAGCTC 1 cut(s) 1021
SapI GCTCTTC 1 cut(s) 47
SaqAI TTAA 3 cut(s) 228, 327, 789
SatI GCNGC 4 cut(s) 597, 1048, 1051, 1163
Sau3AI GATC 5 cut(s) 194, 416, 679, 804, 1135
SchI GAGTC 3 cut(s) 16, 588, 773
SduI GDGCHC 2 cut(s) 949, 1021
SfaNI GCATC 4 cut(s) 583, 785, 1066, 1078
Sfr274I CTCGAG 2 cut(s) 653, 1252
SlaI CTCGAG 2 cut(s) 653, 1252
SmlI CTYRAG 3 cut(s) 392, 653, 1252
SmoI CTYRAG 3 cut(s) 392, 653, 1252
Sse9I AATT 7 cut(s) 405, 758, 786, 1112, 1206, 1238, 1310
SsiI CCGC 1 cut(s) 46
SstI GAGCTC 1 cut(s) 1021
StyI CCWWGG 3 cut(s) 332, 710, 1266
TaaI ACNGT 2 cut(s) 544, 1145
TaiI ACGT 4 cut(s) 114, 498, 999, 1132
TaqI TCGA 9 cut(s) 172, 197, 577, 582, 654, 810, 816, 892, 1253
TasI AATT 7 cut(s) 405, 758, 786, 1112, 1206, 1238, 1310
TatI WGTACW 1 cut(s) 762
TfiI GAWTC 2 cut(s) 438, 830
Tru1I TTAA 3 cut(s) 228, 327, 789
Tru9I TTAA 3 cut(s) 228, 327, 789
TscAI CASTG 3 cut(s) 525, 847, 1114
TseFI GTSAC 1 cut(s) 560
TseI GCWGC 4 cut(s) 596, 1047, 1050, 1162
Tsp45I GTSAC 1 cut(s) 560
TspDTI ATGAA 8 cut(s) 136, 140, 278, 417, 1172, 1215, 1219, 1332
TspRI CASTG 3 cut(s) 525, 847, 1114
XapI RAATTY 3 cut(s) 1112, 1238, 1310
XcmI CCANNNNNNNNNTGG 1 cut(s) 72
XhoI CTCGAG 2 cut(s) 653, 1252
Zsp2I ATGCAT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.