Rorug04G0256900
TCP Family

TCP-1/cpn60 chaperonin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0015408
Physical Location & Seq
Forward (+)
42928927 .. 42932376
3450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0256900.1

Sequence Viewer

Length: 1209 bp
ATGGAGAACATTGTGTCTGAGAATGCATTGGTGGTGATAGTAGGCGCCGGCCCCTCAGGCATTGCAACCTCAGCGCTACTCAACTCCATGTCGGTGTCAAACATTGTTTTTGAGAGGGAAGACTGTTGTGCTTCCCTTTGGAAGAAACGGTCCTACGACCGTTTGAGTCTTCATTTGGCAAAAAAATTTTGCTCTCTTCCTCTTATGCCGCACCCATTTAGCACTGCAACTTTCATGTCCAAAGACACGTTCATAGACTATGTGGATGAGTATGTTTCCCGTTTCAATGTCAACCCCCGTTATTGTCACAATGTTGAGTCGGCTTTTTACGAAGAAGCTGATCAGAAATGGAAGGTTGAAGTGAAGAACACTAGGGCGGCTGATAAGGAGGTTTCCTTTCAAGTGTACAACGCAGATTTCTTGGTGATTGCCACCGGAGAAAATAGCCAGGCGATTATCCCAGAGTTGCCTGGACTGGAGACCTTTAAAGGAAAGGTTATTCATGCGAGTGATTATAAATGTGGAGCGAGTTTGAAAGATAAGAGTGTGTTGGTTATTGGTTGTGGCAATTCTGGCATGGAGATTAGTAATGATCTCTCGGAGAATGGAGCTCATGCATGCATTGTTGTTAGGAGCCAACTTCATGTACTCAGCAGAGAAATTGTACGGATTGGGATGGTACTGTTGGATTTCCTCCCGGTGAATATGGTGGACAAGTTCGTTTCATTTCTCGCCAAGTTTAGCTACAGTGATCTACCCAACTATGGGATTCATCAACCGACTGAAGGTCCTTTCTTCTATAAAGCACTTACGGGAAAGACTCCGGTAATTGATCGTGGAACTATCAAGAAAATTCGCTCCAGAAAGATTAAGGTTTTCCCAGGAATAGAAAAGATACACGACAATATTGTGGAATTCAAAAATGGTGCCCTCCAGTGTTTTGATGCCATTGTGTTGGCAACTGGTTATCGAAGCGTAGCACACAACTGGCTCAAGGATTATAAGCTCATTCTTGATGATGACGACAAGCCTAAGAACAGGTATCCATACCATTGGAAAGGAGAGAAGGGCCTTTACTGTGTTGGATTTTCTGGGAGAGGGATTCAGGGAATCTCGTCGGATTCTAAGGCTGTTGCTCAAGATATCTATCGAATTTTAATGGCACAGAAGTTCGCTTATGCTCCAGGCTCCAGCAATGGTTTAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cpn60_TCP1

Protein Analysis

402

Amino Acids

44.79

Weight (kDa)

8.41

Isoelectric Point (pI)

30.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMO-like PF00743 11 - 332 7e-22 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 11 - 212 5.2e-14 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 12 - 213 1.8e-17 Pyridine nucleotide-disulphide oxidoreductase
Lys_Orn_oxgnase PF13434 75 - 208 5.3e-12 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015408)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24510 AT1G24510 AT1G24510
fragaria_vesca FvH4_5g28870
malus_domestica MD13G1106100.v1.1 MD16G1106300.v1.1
prunus_persica Prupe.1G240000_v2.0.a1
pyrus_communis pycom13g09210 pycom16g09050
rosa_chinensis RchiOBHm_Chr4g0432301
rosa_laevigata RLG00000006854
rosa_multiflora Rmu_sc0001084.1_g000043
rosa_roxburghii Rroxscaffold_5G00373760
rosa_rugosa Rorug04G0256900
rosa_samantha Rh4DG316300
rosa_wichuraiana Rw4G027230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 516, 1002
AccB1I GGYRCC 2 cut(s) 44, 926
AciI CCGC 2 cut(s) 209, 377
AcsI RAATTY 4 cut(s) 185, 852, 914, 1152
AcuI CTGAAG 1 cut(s) 804
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 4 cut(s) 407, 648, 666, 681
AfeI AGCGCT 1 cut(s) 75
AfiI CCNNNNNNNGG 3 cut(s) 764, 765, 785
AflIII ACRYGT 1 cut(s) 246
AgsI TTSAA 5 cut(s) 286, 359, 401, 535, 919
AhdI GACNNNNNGTC 1 cut(s) 786
AjnI CCWGG 4 cut(s) 447, 469, 880, 1183
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
AluBI AGCT 4 cut(s) 338, 611, 744, 1006
AluI AGCT 4 cut(s) 338, 611, 744, 1006
Alw21I GWGCWC 1 cut(s) 613
Alw26I GTCTC 1 cut(s) 473
Aor51HI AGCGCT 1 cut(s) 75
AoxI GGCC 2 cut(s) 49, 1069
ApoI RAATTY 4 cut(s) 185, 852, 914, 1152
AspLEI GCGC 2 cut(s) 47, 76
AspS9I GGNCC 4 cut(s) 50, 150, 788, 1069
AsuC2I CCSGG 1 cut(s) 698
AsuHPI GGTGA 3 cut(s) 46, 436, 712
AvaII GGWCC 2 cut(s) 150, 788
AxyI CCTNAGG 1 cut(s) 55
BaeGI GKGCMC 1 cut(s) 931
BanI GGYRCC 2 cut(s) 44, 926
BanII GRGCYC 1 cut(s) 613
BarI GAAGNNNNNNTAC 2 cut(s) 1058, 1090
BbsI GAAGAC 2 cut(s) 126, 161
Bbv12I GWGCWC 1 cut(s) 613
BbvCI CCTCAGC 1 cut(s) 70
BccI CCATC 1 cut(s) 670
BciT130I CCWGG 4 cut(s) 449, 471, 882, 1185
BciVI GTATCC 1 cut(s) 1053
BclI TGATCA 1 cut(s) 340
BcnI CCSGG 1 cut(s) 698
BcoDI GTCTC 1 cut(s) 473
BfaI CTAG 1 cut(s) 372
BfmI CTRYAG 1 cut(s) 745
BfoI RGCGCY 2 cut(s) 48, 77
BfuI GTATCC 1 cut(s) 1053
BglI GCCNNNNNGGC 1 cut(s) 57
BisI GCNGC 2 cut(s) 209, 378
BlsI GCNGC 2 cut(s) 210, 379
Bme1390I CCNGG 5 cut(s) 449, 471, 698, 882, 1185
Bme18I GGWCC 2 cut(s) 150, 788
BmeRI GACNNNNNGTC 1 cut(s) 786
BmgT120I GGNCC 4 cut(s) 50, 150, 788, 1069
BmiI GGNNCC 5 cut(s) 46, 52, 635, 928, 1189
BmrFI CCNGG 5 cut(s) 449, 471, 698, 882, 1185
BmsI GCATC 1 cut(s) 934
BpiI GAAGAC 2 cut(s) 126, 161
BpmI CTGGAG 5 cut(s) 497, 844, 917, 1167, 1174
Bpu10I CCTNAGC 1 cut(s) 70
BpuEI CTTGAG 2 cut(s) 977, 1122
BpuMI CCSGG 1 cut(s) 698
BsaBI GATNNNNATC 1 cut(s) 1146
BsaHI GRCGYC 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 1 cut(s) 880
BsaWI WCCGGW 2 cut(s) 434, 823
Bsc4I CCNNNNNNNGG 3 cut(s) 764, 765, 785
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 4 cut(s) 480, 934, 967, 992
Bse21I CCTNAGG 1 cut(s) 55
Bse3DI GCAATG 2 cut(s) 60, 1201
Bse8I GATNNNNATC 1 cut(s) 1146
BseBI CCWGG 4 cut(s) 449, 471, 882, 1185
BseDI CCNNGG 1 cut(s) 880
BseGI GGATG 2 cut(s) 271, 681
BseJI GATNNNNATC 1 cut(s) 1146
BseLI CCNNNNNNNGG 3 cut(s) 764, 765, 785
BseMI GCAATG 2 cut(s) 60, 1201
BseMII CTCAG 4 cut(s) 9, 69, 84, 664
BseNI ACTGG 4 cut(s) 480, 934, 967, 992
BseSI GKGCMC 1 cut(s) 931
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 2 cut(s) 51, 1071
BshNI GGYRCC 2 cut(s) 44, 926
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 1 cut(s) 613
BsiSI CCGG 4 cut(s) 48, 435, 698, 824
BslI CCNNNNNNNGG 3 cut(s) 764, 765, 785
BsmAI GTCTC 1 cut(s) 473
BsmI GAATGC 1 cut(s) 28
BsnI GGCC 2 cut(s) 51, 1071
Bso31I GGTCTC 1 cut(s) 473
Bsp1286I GDGCHC 2 cut(s) 613, 931
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 4 cut(s) 340, 592, 751, 832
BspACI CCGC 2 cut(s) 209, 377
BspANI GGCC 2 cut(s) 51, 1071
BspCNI CTCAG 4 cut(s) 10, 68, 83, 663
BspLI GGNNCC 5 cut(s) 46, 52, 635, 928, 1189
BspT107I GGYRCC 2 cut(s) 44, 926
BspTNI GGTCTC 1 cut(s) 473
BsrDI GCAATG 2 cut(s) 60, 1201
BsrFI RCCGGY 1 cut(s) 47
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 4 cut(s) 480, 934, 967, 992
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 1 cut(s) 880
BssMI GATC 4 cut(s) 340, 592, 751, 832
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 4 cut(s) 449, 471, 882, 1185
Bst4CI ACNGT 6 cut(s) 125, 150, 161, 684, 749, 1079
Bst6I CTCTTC 1 cut(s) 201
BstACI GRCGYC 1 cut(s) 45
BstAUI TGTACA 1 cut(s) 405
BstC8I GCNNGC 2 cut(s) 49, 619
BstDEI CTNAG 6 cut(s) 18, 55, 70, 650, 1032, 1125
BstF5I GGATG 2 cut(s) 271, 681
BstH2I RGCGCY 2 cut(s) 48, 77
BstHHI GCGC 2 cut(s) 47, 76
BstKTI GATC 4 cut(s) 343, 595, 754, 835
BstMAI GTCTC 1 cut(s) 473
BstMBI GATC 4 cut(s) 340, 592, 751, 832
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 3 cut(s) 57, 71, 573
BstNI CCWGG 4 cut(s) 449, 471, 882, 1185
BstNSI RCATGY 1 cut(s) 621
BstSCI CCNGG 5 cut(s) 447, 469, 696, 880, 1183
BstSFI CTRYAG 1 cut(s) 745
BstSLI GKGCMC 1 cut(s) 931
BstV2I GAAGAC 2 cut(s) 126, 161
BstXI CCANNNNNNTGG 2 cut(s) 955, 1053
Bsu36I CCTNAGG 1 cut(s) 55
BsuI GTATCC 1 cut(s) 1053
BsuRI GGCC 2 cut(s) 51, 1071
BtsCI GGATG 2 cut(s) 271, 681
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 3 cut(s) 222, 754, 941
Cac8I GCNNGC 2 cut(s) 49, 619
CfoI GCGC 2 cut(s) 47, 76
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 4 cut(s) 50, 150, 788, 1069
Csp6I GTAC 4 cut(s) 406, 647, 665, 680
CviAII CATG 7 cut(s) 88, 235, 503, 577, 614, 618, 644
CviQI GTAC 4 cut(s) 406, 647, 665, 680
DdeI CTNAG 6 cut(s) 18, 55, 70, 650, 1032, 1125
DinI GGCGCC 1 cut(s) 46
DpnI GATC 4 cut(s) 342, 594, 753, 834
DpnII GATC 4 cut(s) 340, 592, 751, 832
DraI TTTAAA 1 cut(s) 487
DriI GACNNNNNGTC 1 cut(s) 786
Eam1104I CTCTTC 1 cut(s) 201
Eam1105I GACNNNNNGTC 1 cut(s) 786
EarI CTCTTC 1 cut(s) 201
Ecl136II GAGCTC 1 cut(s) 611
Eco24I GRGCYC 1 cut(s) 613
Eco31I GGTCTC 1 cut(s) 473
Eco32I GATATC 1 cut(s) 1144
Eco47I GGWCC 2 cut(s) 150, 788
Eco47III AGCGCT 1 cut(s) 75
Eco53kI GAGCTC 1 cut(s) 611
Eco57I CTGAAG 1 cut(s) 804
Eco81I CCTNAGG 1 cut(s) 55
EcoICRI GAGCTC 1 cut(s) 611
EcoO109I RGGNCCY 2 cut(s) 788, 1069
EcoRI GAATTC 1 cut(s) 914
EcoRII CCWGG 4 cut(s) 447, 469, 880, 1183
EcoRV GATATC 1 cut(s) 1144
EcoT22I ATGCAT 3 cut(s) 28, 619, 623
EcoT38I GRGCYC 1 cut(s) 613
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
FaeI CATG 7 cut(s) 91, 238, 506, 580, 617, 621, 647
FatI CATG 7 cut(s) 87, 234, 502, 576, 613, 617, 643
FbaI TGATCA 1 cut(s) 340
Fnu4HI GCNGC 2 cut(s) 209, 378
FokI GGATG 2 cut(s) 278, 688
FriOI GRGCYC 1 cut(s) 613
Fsp4HI GCNGC 2 cut(s) 209, 378
FspBI CTAG 1 cut(s) 372
GlaI GCGC 2 cut(s) 46, 75
GluI GCNGC 2 cut(s) 209, 378
GsuI CTGGAG 5 cut(s) 497, 844, 917, 1167, 1174
HaeII RGCGCY 2 cut(s) 48, 77
HaeIII GGCC 2 cut(s) 51, 1071
HapII CCGG 4 cut(s) 48, 435, 698, 824
HhaI GCGC 2 cut(s) 47, 76
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 7 cut(s) 91, 238, 506, 580, 617, 621, 647
Hin6I GCGC 2 cut(s) 45, 74
HinP1I GCGC 2 cut(s) 45, 74
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 7 cut(s) 166, 317, 769, 820, 1102, 1110, 1121
HpaII CCGG 4 cut(s) 48, 435, 698, 824
HphI GGTGA 3 cut(s) 46, 436, 712
Hpy166II GTNNAC 3 cut(s) 292, 406, 712
Hpy188I TCNGA 4 cut(s) 19, 345, 601, 1120
Hpy188III TCNNGA 4 cut(s) 847, 861, 1013, 1139
Hpy8I GTNNAC 3 cut(s) 292, 406, 712
Hpy99I CGWCG 1 cut(s) 1120
HpyAV CCTTC 3 cut(s) 346, 779, 1060
HpyCH4III ACNGT 6 cut(s) 125, 150, 161, 684, 749, 1079
HpyCH4IV ACGT 1 cut(s) 248
HpyCH4V TGCA 5 cut(s) 26, 65, 227, 617, 621
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 71, 573
HpyF3I CTNAG 6 cut(s) 18, 55, 70, 650, 1032, 1125
HpySE526I ACGT 1 cut(s) 248
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 7 cut(s) 91, 238, 506, 580, 617, 621, 647
HspAI GCGC 2 cut(s) 45, 74
KasI GGCGCC 1 cut(s) 44
KroI GCCGGC 1 cut(s) 47
KroNI GCCGGC 1 cut(s) 49
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 4 cut(s) 340, 592, 751, 832
LmnI GCTCC 6 cut(s) 524, 608, 633, 863, 1186, 1193
LweI GCATC 1 cut(s) 934
MaeI CTAG 1 cut(s) 372
MaeII ACGT 1 cut(s) 248
MaeIII GTNAC 1 cut(s) 305
MalI GATC 4 cut(s) 342, 594, 753, 834
MboI GATC 4 cut(s) 340, 592, 751, 832
MboII GAAGA 7 cut(s) 131, 154, 161, 188, 344, 376, 787
MhlI GDGCHC 2 cut(s) 613, 931
MluCI AATT 7 cut(s) 185, 568, 660, 828, 852, 914, 1152
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 3 cut(s) 175, 326, 814
MmeI TCCRAC 3 cut(s) 666, 1063, 1098
MnlI CCTC 8 cut(s) 64, 79, 108, 210, 382, 704, 941, 1091
Mph1103I ATGCAT 3 cut(s) 28, 619, 623
MroNI GCCGGC 1 cut(s) 47
MseI TTAA 3 cut(s) 486, 870, 1157
MslI CAYNNNNRTG 2 cut(s) 92, 507
MspI CCGG 4 cut(s) 48, 435, 698, 824
MspR9I CCNGG 5 cut(s) 449, 471, 698, 882, 1185
Mva1269I GAATGC 1 cut(s) 28
MvaI CCWGG 4 cut(s) 449, 471, 882, 1185
MwoI GCNNNNNNNGC 3 cut(s) 57, 71, 573
NaeI GCCGGC 1 cut(s) 49
NarI GGCGCC 1 cut(s) 45
NciI CCSGG 1 cut(s) 698
NdeII GATC 4 cut(s) 340, 592, 751, 832
NgoMIV GCCGGC 1 cut(s) 47
NlaIII CATG 7 cut(s) 91, 238, 506, 580, 617, 621, 647
NlaIV GGNNCC 5 cut(s) 46, 52, 635, 928, 1189
NmuCI GTSAC 1 cut(s) 305
NsiI ATGCAT 3 cut(s) 28, 619, 623
NspI RCATGY 1 cut(s) 621
PaeI GCATGC 1 cut(s) 621
PctI GAATGC 1 cut(s) 28
PdiI GCCGGC 1 cut(s) 49
PfeI GAWTC 4 cut(s) 769, 1102, 1110, 1121
PkrI GCNGC 2 cut(s) 210, 379
PleI GAGTC 3 cut(s) 174, 325, 814
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 3 cut(s) 174, 325, 814
PpuMI RGGWCCY 1 cut(s) 788
PsiI TTATAA 2 cut(s) 516, 1002
Psp124BI GAGCTC 1 cut(s) 613
Psp5II RGGWCCY 1 cut(s) 788
Psp6I CCWGG 4 cut(s) 447, 469, 880, 1183
PspGI CCWGG 4 cut(s) 447, 469, 880, 1183
PspN4I GGNNCC 5 cut(s) 46, 52, 635, 928, 1189
PspPI GGNCC 4 cut(s) 50, 150, 788, 1069
PspPPI RGGWCCY 1 cut(s) 788
RsaI GTAC 4 cut(s) 407, 648, 666, 681
RsaNI GTAC 4 cut(s) 406, 647, 665, 680
RseI CAYNNNNRTG 2 cut(s) 92, 507
SacI GAGCTC 1 cut(s) 613
SaqAI TTAA 3 cut(s) 486, 870, 1157
SatI GCNGC 2 cut(s) 209, 378
Sau3AI GATC 4 cut(s) 340, 592, 751, 832
Sau96I GGNCC 4 cut(s) 50, 150, 788, 1069
SchI GAGTC 3 cut(s) 175, 326, 814
ScrFI CCNGG 5 cut(s) 449, 471, 698, 882, 1185
SduI GDGCHC 2 cut(s) 613, 931
SfaNI GCATC 1 cut(s) 934
SfcI CTRYAG 1 cut(s) 745
SfoI GGCGCC 1 cut(s) 46
SinI GGWCC 2 cut(s) 150, 788
SmiMI CAYNNNNRTG 2 cut(s) 92, 507
SmlI CTYRAG 2 cut(s) 992, 1137
SmoI CTYRAG 2 cut(s) 992, 1137
SphI GCATGC 1 cut(s) 621
Sse9I AATT 7 cut(s) 185, 568, 660, 828, 852, 914, 1152
SsiI CCGC 2 cut(s) 209, 377
SspDI GGCGCC 1 cut(s) 44
SspI AATATT 1 cut(s) 907
SspMI CTAG 1 cut(s) 372
SstI GAGCTC 1 cut(s) 613
StyD4I CCNGG 5 cut(s) 447, 469, 696, 880, 1183
TaaI ACNGT 6 cut(s) 125, 150, 161, 684, 749, 1079
TaiI ACGT 1 cut(s) 251
TaqI TCGA 2 cut(s) 970, 1150
TasI AATT 7 cut(s) 185, 568, 660, 828, 852, 914, 1152
TatI WGTACW 2 cut(s) 405, 646
TauI GCSGC 2 cut(s) 211, 380
TfiI GAWTC 4 cut(s) 769, 1102, 1110, 1121
Tru1I TTAA 3 cut(s) 486, 870, 1157
Tru9I TTAA 3 cut(s) 486, 870, 1157
TscAI CASTG 3 cut(s) 229, 754, 941
TseFI GTSAC 1 cut(s) 305
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 7 cut(s) 161, 223, 241, 491, 632, 714, 761
TspGWI ACGGA 1 cut(s) 682
TspRI CASTG 3 cut(s) 229, 754, 941
VpaK11BI GGWCC 2 cut(s) 150, 788
XapI RAATTY 4 cut(s) 185, 852, 914, 1152
XceI RCATGY 1 cut(s) 621
XspI CTAG 1 cut(s) 372
Zsp2I ATGCAT 3 cut(s) 28, 619, 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.