Rorug04G0261400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009583
Physical Location & Seq
Reverse (-)
43340878 .. 43345809
4932 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0261400.1

Sequence Viewer

Length: 3876 bp
ATGGCAGAATTTCTGCTCACTTTTGCTGCCGAGGGAATATTGACCAAGGTGACTTCTCTTGCTGCTCAAGAATTCAGTCTCGTATTGGATTTCAAAGAAGATCTAGCACAGCTGCGCGATTCGTTCCTCAATATTCAAGCTATGCTAAGAGATGTCGACCATTCACAAGTTCGAGGGGAGGCTGTGGAAAATTGGGTGAAGGATCTTGAAGACATAGCTCACAAAGCCGACGATGTGTTGGATGAAATTGCGTATGAAGATCTCCGACGCAAAGTAGAACTCCAAGACCGGATGAAGAAAAAGGTGAAAAATTTCTTCTCCCACTCCAATCCCATTGCATTTCGCTTAGAAATGGCACATAAAATCAAAAAAATCAATACATCTTTGGTGAAATTATTCAATCAGGCTGCTGGCCCTATTGGGTTAGTTGCTAGGACATTAGCAGATGCAACCTCTCAAGATATTGAAGTACTTGACAGGGAGACCATCTCCAGCTTTGATAGCGATGAGAAGCTCATCATTGGAAGGAAAGAGGTCGTGTCAGATATAGTCAAAACCTTACTCAAGTCACACTATAATGAAGAAAATTATCTTCCGGTTTTGGCTATTGTGGGAATGCCGGGTCTGGGGAAGACAACTTTGGCTAAAGCAATATATCATGAATCTAAGATTGATAGCCACTTCCATGAAAAAATATGGGTGTGTGTATCCACCCCTTTTGAAGTCAAGACGATCTTAAGGGCGATCTTGGAAACTCTGAAACCAGAGAAAGCAGCAATACAAAGCAAGGACGCGATTTGTAAATTCATTCGAGAAGAGGTAAAAGGGAAAAGATACCTTCTCGTATTGGATGATGTTTGGAGCGAAGATCCAGAAAAATGGAAGGAGTTGAGGAGTTGTTTGTTAACAATTAATGATACTCAAGGAAGCAGCATCATTGTCACTACCCGTAGTGACAGAGTTGCAAAAGTCATGGAAACACTTCCTAGATGTGATTTAAGAAAACTATCAGATGATGAATGCTGGCTCATTTTGAAGGATAAAGCCATTCCAGTTGGGAGTCCTCCTATGTCTAAAGAGCAGGAGACAATTGGTAAAGAGATCGCTAAAAGATGTGGAGGTCTACCACTAGTGTCAAAGGTTTTGGGAGATGTGATGCGCTCTAAAACAAGTGATGAATGGCGATCAATTTTAAAAAGTAAAACATGGGATTCACCAGAAGGAGAAAAGAGAATTTTTTCAATTTTGAAGTTGAGTTTTGATGAACTGAAACCTCCATCCTTGAAACAATGTTTTGCATATTGCTCGATGTTCTTCAAAGATTTTGATATTGAAAAGGATGACTTGATCCAACTTTGGATGGCTCAGGGATGGCTTCACCCTTATTCTGACAAAAGCCTAGAGATGGAGGAGAGAGGTAATGAATATTTTAAAATCTTATTGGCGAAGTCTTTTTTTCAAGACGTTACAAAGGGTTATGGTGGCAATGTTACCAAATGTAAGATGCACGATCTTGTGCATGATCTTGCAGAACATGTATCAAAATCAAGGAACTTACGCTCACTTTTTTCAAATGGAGAAGTCCTTGGGAGCAACTTACTTAACTTCAAATCTTTACGTGTGTTAAATTTATACAAAGCAGACATTATGGAGTTGCCAATTTCAATTGGGAAGTTGAAACACTTGAGGTATTTGAATGTTTTGAAAACAAGAATCAAAACATTTCCCAAATCAATTGGTCAGCTTTATAACCTGCAAACATTGAAAATACCTTATCAGCTTGAAGAATTTCCAAGGGAAATTGCAAATTTGATCAACTTGCGGCACATTTATTTTGGTAGATATATGAAAGTTCCAGGTGAGATATTGAGACGATTGACTAATCTCCGGTCATTACCTTTTCTCAAGGTGGGTAAGGAGACGGGTCCTAGAATTGAGGAACTGGGTTGTTTAAACCAGTTGCACGACACCTTGTCTATATATGGACTGGAAAATGTAGGAGATGGAGAAGAAGCTCTGAAAGCAAACTTGGTAGAGAAGAAACATATGCGCAGGTTAATCCTTGGATGGAAGCTTAGCAGGCCAAGCCACAATGTGGAGAATGACGACAATGTACTAGAAAGCCTCCAACCACATTCTAGTTTGGGATTTTTGGAGATTGAAGGATTTATGGGTGTTAAATTTCCATCATGGTTATTGCTAGCCAACAATTTGAAAGAGATTGAATTATCGGGCTGCAACAAATGTGAAGGAGTCCCAGTACTTGGGCATTTACCCAATCTTAGTTGTGTTAAGATTAAGAGAATGGAGAACCTAACTCGTATAGGACCTGAGTTCTATGGTGATGACCGTGTTAACTGTGGAAATGGATCAAGTAACGAGGCGCGATCTTTGTTCCCTGCTTTGAAAACATTGCATATTGAGGGGGCCCAGAACCTGATTGAATGGATGGAAGCGCCAACAGGAAGAGCAAGTGTGGTGTTTCCATGCCTTGAGCAGCTGACCTTGATTCGGTGTAACAAACTGACAACTGCTCCCAGTCATTTCCCATCTCTCAAGAAATTGGTGATAGAAGACATGGAGAGTGGAGGCATGCCAATAGCAAGTATTCTAAGCAATCAACTCACCGCTCTCACTTATCTCAGCTTAGAAGGTGTGGGGGGACTTACTTGTCTGCCGGAAGGGATGTTAGAAAACAATGCGAATCTTGCACATTTGGAAATGTGGACTTGTTCGGAGTTAATTTGTATTTCTCCCCAATCACACGGATTTGAGTACTGCTGCGCATCTCTTTCATATTTGGGGATATTTATTTGTAAGAATCTCAGATATTTACCTGATGGGCTACTCACTCCTTCTCTTAAAGAGATGACGTTGTATAATTGTCCCAATCTAGAGTACATCTCAGATGCTACACACGGTGGTCTCACATCCCTTGAAACATTGAAGGTGATGCAGTGCCCTAAAATAACTTCCATTCCATTTTCACAAGGCCTCCCATCTCTTGGTGAATTCACTATAAGCAGGTGTCGGGAATTATCAAGCCTACCGGGTGGACTAGAATACTGTACCTCTATTCGGAGTTTGTCAATAACAGCTTGCCCCAAGGTAGCATCCATTTCAATCGAAAGTCTGAGTGCATCCCTCGAAGATTTGTGTGTAAGTAATCTACACTCTCTTCCAATTTCACGAGGAGGCTTCACATCACTCCGTCAATTGAGAATTGAGCGCAGCGAAAGTGCCCAAATCGGGCCAGAATTTAGTGCCTTTCTTCAAACCCTCGTCTCTCTTCAAAAATTGACGATGAGGTTTTGCAAAAATCTAGAGACTATTCCAAGTTCAGACAAGCTCACATCCCTCCGCAGCTTGGAGATTATTGGGTGTAACAAATTAACATGTCTACCGAATGGGTTAGCAGCATCGTCACAGTCCTGCAGCCTCACCCGTTTGAAGCACTTGACAATTGGTCGTTTCTGCGAGGATCTCGATGCATTCCCAGCTTTTCAGCCTATACCACAGCTTGAATCATTAAGCATCTACGGGTGGCGTAAGCTCAAGTCTCTCCCTGAACAAATTCAACACTTGCCTTCTTTAAGGCATTTGTTTATAGCATCCTTTGAGGGAGTGGAGGCTTTTCCAGAGTGGTTGGGAAACCTTGCATCTCTTGAGGACCTGACTGTTGGGTCTTGCGAGAGTCTAAAGTATCTACCTTCTGTGGAAGCTATGCAATGCCTCACCAAATTGAAGGAGATACGCATCATCTACAGTCCCCTTCTAGAAGAAAGATGCAGGGAGGAGAGAGGCCCAGAGTGGCCTAAGATTCGTCATCTTCCATTCATCACGAGTAAGACTTGTTCTCATCTATTACTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1291

Amino Acids

145.45

Weight (kDa)

6.88

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 13 - 99 6.2e-21 Rx N-terminal domain
nSTAND1 PF20703 168 - 390 3.2e-08 Novel STAND NTPase 1
NB-ARC PF00931 179 - 350 2.9e-35 NB-ARC domain
AAA_22 PF13401 200 - 303 3.2e-06 AAA domain
WHD_DRP PF23559 437 - 510 9.7e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 520 - 771 2.9e-27 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 645 - 769 1.1e-34 R13L1/DRL21 LRRs
LRR_14 PF23598 1051 - 1203 2e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 1116 - 1254 9.1e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_14 PF23598 1174 - 1262 2.2e-07 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 1190 - 1249 1.7e-06 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1749
AarI CACCTGC 1 cut(s) 3015
AasI GACNNNNNNGTC 2 cut(s) 2670, 3682
Acc16I TGCGCA 2 cut(s) 2051, 2785
Acc36I ACCTGC 3 cut(s) 1761, 2043, 3015
AccB7I CCANNNNNTGG 3 cut(s) 2095, 2264, 3005
AccBSI CCGCTC 1 cut(s) 2630
AccI GTMKAC 3 cut(s) 156, 1123, 3400
AccII CGCG 3 cut(s) 117, 794, 2386
AciI CCGC 3 cut(s) 1822, 2628, 3361
AclWI GGATC 5 cut(s) 210, 863, 1342, 2377, 3489
AdeI CACNNNGTG 2 cut(s) 2095, 2921
AfaI GTAC 6 cut(s) 471, 2115, 2262, 2777, 2900, 3070
AfiI CCNNNNNNNGG 9 cut(s) 626, 1405, 2095, 2264, 2511, 3005, 3078, 3249, 3367
AflII CTTAAG 1 cut(s) 736
AflIII ACRYGT 3 cut(s) 1534, 1618, 3395
AhdI GACNNNNNGTC 2 cut(s) 1972, 3465
AhlI ACTAGT 1 cut(s) 1129
AjnI CCWGG 1 cut(s) 1855
AjuI GAANNNNNNNTTGG 4 cut(s) 623, 655, 2012, 2044
Alw26I GTCTC 9 cut(s) 83, 476, 1079, 1864, 1913, 2930, 3289, 3320, 3564
AlwI GGATC 5 cut(s) 210, 863, 1342, 2377, 3489
AlwNI CAGNNNCTG 1 cut(s) 2437
AoxI GGCC 7 cut(s) 412, 2081, 2427, 2992, 3251, 3802, 3812
ApaI GGGCCC 1 cut(s) 2431
AseI ATTAAT 1 cut(s) 912
Asp700I GAANNNNTTC 6 cut(s) 311, 395, 981, 1237, 1788, 3573
AspLEI GCGC 7 cut(s) 117, 1161, 2052, 2386, 2458, 2786, 3231
AspS9I GGNCC 8 cut(s) 413, 1925, 2327, 2427, 2428, 3251, 3670, 3803
AsuC2I CCSGG 2 cut(s) 621, 3051
AsuNHI GCTAGC 1 cut(s) 2200
AvaII GGWCC 3 cut(s) 1925, 2327, 3670
BaeGI GKGCMC 3 cut(s) 2431, 2963, 3244
BanII GRGCYC 1 cut(s) 2431
BauI CACGAG 2 cut(s) 3189, 3841
BbsI GAAGAC 3 cut(s) 216, 638, 2580
BcgI CGANNNNNNTGC 2 cut(s) 1287, 1321
BciT130I CCWGG 1 cut(s) 1857
BciVI GTATCC 1 cut(s) 718
BclI TGATCA 1 cut(s) 1812
BcnI CCSGG 2 cut(s) 621, 3051
BcoDI GTCTC 9 cut(s) 83, 476, 1079, 1864, 1913, 2930, 3289, 3320, 3564
BcuI ACTAGT 1 cut(s) 1129
BfmI CTRYAG 2 cut(s) 3433, 3763
BfoI RGCGCY 1 cut(s) 2459
BfrI CTTAAG 1 cut(s) 736
BfuAI ACCTGC 3 cut(s) 1761, 2043, 3015
BfuI GTATCC 1 cut(s) 718
BglII AGATCT 2 cut(s) 100, 259
BlpI GCTNAGC 1 cut(s) 2075
BmcAI AGTACT 3 cut(s) 471, 2262, 2777
Bme1390I CCNGG 3 cut(s) 621, 1857, 3051
Bme18I GGWCC 3 cut(s) 1925, 2327, 3670
BmeRI GACNNNNNGTC 2 cut(s) 1972, 3465
BmgT120I GGNCC 8 cut(s) 413, 1925, 2327, 2427, 2428, 3251, 3670, 3803
BmiI GGNNCC 3 cut(s) 1926, 2428, 2429
BmrFI CCNGG 3 cut(s) 621, 1857, 3051
BmrI ACTGGG 3 cut(s) 1952, 2252, 2532
BmtI GCTAGC 1 cut(s) 2204
BmuI ACTGGG 3 cut(s) 1952, 2252, 2532
BpiI GAAGAC 3 cut(s) 216, 638, 2580
BplI GAGNNNNNCTC 4 cut(s) 473, 505, 2888, 2920
BpmI CTGGAG 1 cut(s) 475
Bpu10I CCTNAGC 1 cut(s) 1365
Bpu1102I GCTNAGC 1 cut(s) 2075
BpuMI CCSGG 2 cut(s) 621, 3051
BsaAI YACGTR 1 cut(s) 1619
BsaBI GATNNNNATC 1 cut(s) 3755
BsaI GGTCTC 2 cut(s) 476, 2930
BsaJI CCNNGG 6 cut(s) 30, 45, 1585, 1793, 2062, 3105
BsaWI WCCGGW 3 cut(s) 288, 595, 1887
BsaXI ACNNNNNCTCC 6 cut(s) 2244, 2274, 2518, 2548, 2979, 3009
Bsc4I CCNNNNNNNGG 9 cut(s) 626, 1405, 2095, 2264, 2511, 3005, 3078, 3249, 3367
Bse1I ACTGG 6 cut(s) 1052, 1947, 1957, 1992, 2258, 2538
Bse3DI GCAATG 4 cut(s) 333, 1492, 2411, 3734
Bse8I GATNNNNATC 1 cut(s) 3755
BseBI CCWGG 1 cut(s) 1857
BseDI CCNNGG 6 cut(s) 30, 45, 1585, 1793, 2062, 3105
BseJI GATNNNNATC 1 cut(s) 3755
BseLI CCNNNNNNNGG 9 cut(s) 626, 1405, 2095, 2264, 2511, 3005, 3078, 3249, 3367
BseMI GCAATG 4 cut(s) 333, 1492, 2411, 3734
BseMII CTCAG 6 cut(s) 1379, 2322, 2656, 2839, 2919, 3125
BseNI ACTGG 6 cut(s) 1052, 1947, 1957, 1992, 2258, 2538
BseRI GAGGAG 4 cut(s) 907, 1424, 3207, 3809
BseSI GKGCMC 3 cut(s) 2431, 2963, 3244
BseYI CCCAGC 1 cut(s) 3496
Bsh1236I CGCG 3 cut(s) 117, 794, 2386
BshFI GGCC 7 cut(s) 414, 2083, 2429, 2994, 3253, 3804, 3814
BsiSI CCGG 6 cut(s) 289, 596, 620, 1888, 2678, 3050
BslFI GGGAC 4 cut(s) 2240, 2676, 2871, 3753
BslI CCNNNNNNNGG 9 cut(s) 626, 1405, 2095, 2264, 2511, 3005, 3078, 3249, 3367
BsmAI GTCTC 9 cut(s) 83, 476, 1079, 1864, 1913, 2930, 3289, 3320, 3564
BsmBI CGTCTC 3 cut(s) 1864, 1913, 3289
BsmFI GGGAC 4 cut(s) 2240, 2676, 2871, 3753
BsmI GAATGC 3 cut(s) 621, 1025, 3491
BsnI GGCC 7 cut(s) 414, 2083, 2429, 2994, 3253, 3804, 3814
Bso31I GGTCTC 2 cut(s) 476, 2930
Bsp120I GGGCCC 1 cut(s) 2427
Bsp1286I GDGCHC 3 cut(s) 2431, 2963, 3244
Bsp1720I GCTNAGC 1 cut(s) 2075
BspACI CCGC 3 cut(s) 1822, 2628, 3361
BspANI GGCC 7 cut(s) 414, 2083, 2429, 2994, 3253, 3804, 3814
BspCNI CTCAG 6 cut(s) 1378, 2323, 2655, 2838, 2918, 3126
BspFNI CGCG 3 cut(s) 117, 794, 2386
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 3 cut(s) 1926, 2428, 2429
BspMAI CTGCAG 1 cut(s) 3437
BspMI ACCTGC 3 cut(s) 1761, 2043, 3015
BspOI GCTAGC 1 cut(s) 2204
BspPI GGATC 5 cut(s) 210, 863, 1342, 2377, 3489
BspQI GCTCTTC 1 cut(s) 2461
BspTI CTTAAG 1 cut(s) 736
BspTNI GGTCTC 2 cut(s) 476, 2930
BsrBI CCGCTC 1 cut(s) 2630
BsrDI GCAATG 4 cut(s) 333, 1492, 2411, 3734
BsrI ACTGG 6 cut(s) 1052, 1947, 1957, 1992, 2258, 2538
BssECI CCNNGG 6 cut(s) 30, 45, 1585, 1793, 2062, 3105
BssSI CACGAG 2 cut(s) 3189, 3841
BssT1I CCWWGG 5 cut(s) 45, 1585, 1793, 2062, 3105
Bst2BI CACGAG 2 cut(s) 3189, 3841
Bst2UI CCWGG 1 cut(s) 1857
Bst4CI ACNGT 7 cut(s) 2351, 2360, 2921, 3068, 3429, 3679, 3767
Bst6I CTCTTC 4 cut(s) 810, 2461, 3183, 3294
BstAFI CTTAAG 1 cut(s) 736
BstBAI YACGTR 1 cut(s) 1619
BstC8I GCNNGC 6 cut(s) 412, 1025, 2081, 2202, 2594, 3100
BstFNI CGCG 3 cut(s) 117, 794, 2386
BstH2I RGCGCY 1 cut(s) 2459
BstHHI GCGC 7 cut(s) 117, 1161, 2052, 2386, 2458, 2786, 3231
BstMAI GTCTC 9 cut(s) 83, 476, 1079, 1864, 1913, 2930, 3289, 3320, 3564
BstMWI GCNNNNNNNGC 7 cut(s) 224, 501, 2021, 2080, 2085, 2708, 3497
BstNI CCWGG 1 cut(s) 1857
BstNSI RCATGY 3 cut(s) 1538, 2596, 3399
BstSCI CCNGG 3 cut(s) 619, 1855, 3049
BstSFI CTRYAG 2 cut(s) 3433, 3763
BstSLI GKGCMC 3 cut(s) 2431, 2963, 3244
BstUI CGCG 3 cut(s) 117, 794, 2386
BstV2I GAAGAC 3 cut(s) 216, 638, 2580
BstX2I RGATCY 5 cut(s) 100, 202, 259, 868, 3481
BstXI CCANNNNNNTGG 1 cut(s) 879
BstYI RGATCY 5 cut(s) 100, 202, 259, 868, 3481
BsuI GTATCC 1 cut(s) 718
BsuRI GGCC 7 cut(s) 414, 2083, 2429, 2994, 3253, 3804, 3814
BtgZI GCGATG 1 cut(s) 519
BtsI GCAGTG 1 cut(s) 2963
BtsIMutI CAGTG 1 cut(s) 2963
BveI ACCTGC 3 cut(s) 1761, 2043, 3015
Cac8I GCNNGC 6 cut(s) 412, 1025, 2081, 2202, 2594, 3100
CaiI CAGNNNCTG 1 cut(s) 2437
CciI TCATGA 1 cut(s) 658
CfoI GCGC 7 cut(s) 117, 1161, 2052, 2386, 2458, 2786, 3231
Cfr13I GGNCC 8 cut(s) 413, 1925, 2327, 2427, 2428, 3251, 3670, 3803
CseI GACGC 2 cut(s) 276, 800
Csp6I GTAC 6 cut(s) 470, 2114, 2261, 2776, 2899, 3069
CspCI CAANNNNNGTGG 2 cut(s) 700, 735
CviQI GTAC 6 cut(s) 470, 2114, 2261, 2776, 2899, 3069
DraI TTTAAA 3 cut(s) 1194, 1432, 1953
DraIII CACNNNGTG 2 cut(s) 2095, 2921
DrdI GACNNNNNNGTC 2 cut(s) 2670, 3682
DriI GACNNNNNGTC 2 cut(s) 1972, 3465
DseDI GACNNNNNNGTC 2 cut(s) 2670, 3682
Eam1104I CTCTTC 4 cut(s) 810, 2461, 3183, 3294
Eam1105I GACNNNNNGTC 2 cut(s) 1972, 3465
EarI CTCTTC 4 cut(s) 810, 2461, 3183, 3294
Eco130I CCWWGG 5 cut(s) 45, 1585, 1793, 2062, 3105
Eco147I AGGCCT 1 cut(s) 2994
Eco24I GRGCYC 1 cut(s) 2431
Eco31I GGTCTC 2 cut(s) 476, 2930
Eco47I GGWCC 3 cut(s) 1925, 2327, 3670
EcoO109I RGGNCCY 4 cut(s) 1925, 2327, 2427, 3670
EcoRI GAATTC 2 cut(s) 71, 3011
EcoRII CCWGG 1 cut(s) 1855
EcoT14I CCWWGG 5 cut(s) 45, 1585, 1793, 2062, 3105
EcoT22I ATGCAT 1 cut(s) 3493
EcoT38I GRGCYC 1 cut(s) 2431
ErhI CCWWGG 5 cut(s) 45, 1585, 1793, 2062, 3105
Esp3I CGTCTC 3 cut(s) 1864, 1913, 3289
FalI AAGNNNNNCTT 4 cut(s) 719, 751, 1328, 1360
FaqI GGGAC 4 cut(s) 2240, 2676, 2871, 3753
FauNDI CATATG 1 cut(s) 2046
FbaI TGATCA 1 cut(s) 1812
FblI GTMKAC 3 cut(s) 156, 1123, 3400
FriOI GRGCYC 1 cut(s) 2431
FspI TGCGCA 2 cut(s) 2051, 2785
GlaI GCGC 7 cut(s) 116, 1160, 2051, 2385, 2457, 2785, 3230
GsaI CCCAGC 1 cut(s) 3500
GsuI CTGGAG 1 cut(s) 475
HaeII RGCGCY 1 cut(s) 2459
HaeIII GGCC 7 cut(s) 414, 2083, 2429, 2994, 3253, 3804, 3814
HapII CCGG 6 cut(s) 289, 596, 620, 1888, 2678, 3050
HgaI GACGC 2 cut(s) 276, 800
HhaI GCGC 7 cut(s) 117, 1161, 2052, 2386, 2458, 2786, 3231
Hin6I GCGC 7 cut(s) 115, 1159, 2050, 2384, 2456, 2784, 3229
HinP1I GCGC 7 cut(s) 115, 1159, 2050, 2384, 2456, 2784, 3229
HincII GTYRAC 3 cut(s) 157, 906, 2356
HindII GTYRAC 3 cut(s) 157, 906, 2356
HindIII AAGCTT 1 cut(s) 2072
HpaI GTTAAC 2 cut(s) 906, 2356
HpaII CCGG 6 cut(s) 289, 596, 620, 1888, 2678, 3050
Hpy166II GTNNAC 7 cut(s) 157, 906, 1124, 2356, 2727, 3056, 3401
Hpy8I GTNNAC 7 cut(s) 157, 906, 1124, 2356, 2727, 3056, 3401
Hpy99I CGWCG 2 cut(s) 233, 270
HpyCH4III ACNGT 7 cut(s) 2351, 2360, 2921, 3068, 3429, 3679, 3767
HpyCH4IV ACGT 3 cut(s) 1464, 1618, 2873
HpyF10VI GCNNNNNNNGC 7 cut(s) 224, 501, 2021, 2080, 2085, 2708, 3497
HpySE526I ACGT 3 cut(s) 1464, 1618, 2873
HspAI GCGC 7 cut(s) 115, 1159, 2050, 2384, 2456, 2784, 3229
Ksp22I TGATCA 1 cut(s) 1812
KspAI GTTAAC 2 cut(s) 906, 2356
LguI GCTCTTC 1 cut(s) 2461
LmnI GCTCC 3 cut(s) 861, 1590, 2539
MaeII ACGT 3 cut(s) 1464, 1618, 2873
MbiI CCGCTC 1 cut(s) 2630
MfeI CAATTG 5 cut(s) 1089, 1665, 1734, 3215, 3462
MflI RGATCY 5 cut(s) 100, 202, 259, 868, 3481
MhlI GDGCHC 3 cut(s) 2431, 2963, 3244
MlyI GAGTC 3 cut(s) 1069, 2262, 3703
MmeI TCCRAC 4 cut(s) 219, 289, 1375, 2152
Mph1103I ATGCAT 1 cut(s) 3493
MroXI GAANNNNTTC 6 cut(s) 311, 395, 981, 1237, 1788, 3573
MslI CAYNNNNRTG 2 cut(s) 576, 684
MspA1I CMGCKG 2 cut(s) 112, 2500
MspCI CTTAAG 1 cut(s) 736
MspI CCGG 6 cut(s) 289, 596, 620, 1888, 2678, 3050
MspR9I CCNGG 3 cut(s) 621, 1857, 3051
MssI GTTTAAAC 1 cut(s) 1953
MunI CAATTG 5 cut(s) 1089, 1665, 1734, 3215, 3462
Mva1269I GAATGC 3 cut(s) 621, 1025, 3491
MvaI CCWGG 1 cut(s) 1857
MvnI CGCG 3 cut(s) 117, 794, 2386
MwoI GCNNNNNNNGC 7 cut(s) 224, 501, 2021, 2080, 2085, 2708, 3497
NciI CCSGG 2 cut(s) 621, 3051
NdeI CATATG 1 cut(s) 2046
NheI GCTAGC 1 cut(s) 2200
NlaIV GGNNCC 3 cut(s) 1926, 2428, 2429
NmeAIII GCCGAG 1 cut(s) 55
NmuCI GTSAC 5 cut(s) 49, 567, 940, 953, 3423
NsbI TGCGCA 2 cut(s) 2051, 2785
NsiI ATGCAT 1 cut(s) 3493
NspI RCATGY 3 cut(s) 1538, 2596, 3399
PaeI GCATGC 1 cut(s) 2596
PagI TCATGA 1 cut(s) 658
PaqCI CACCTGC 1 cut(s) 3015
PceI AGGCCT 1 cut(s) 2994
PciI ACATGT 2 cut(s) 1534, 3395
PciSI GCTCTTC 1 cut(s) 2461
PctI GAATGC 3 cut(s) 621, 1025, 3491
PdmI GAANNNNTTC 6 cut(s) 311, 395, 981, 1237, 1788, 3573
PfeI GAWTC 9 cut(s) 119, 662, 1211, 1713, 2509, 2704, 2821, 3524, 3820
PflMI CCANNNNNTGG 3 cut(s) 2095, 2264, 3005
PleI GAGTC 3 cut(s) 1068, 2261, 3702
PmeI GTTTAAAC 1 cut(s) 1953
PpsI GAGTC 3 cut(s) 1068, 2261, 3702
Ppu21I YACGTR 1 cut(s) 1619
PpuMI RGGWCCY 3 cut(s) 1925, 2327, 3670
PscI ACATGT 2 cut(s) 1534, 3395
PshBI ATTAAT 1 cut(s) 912
PsiI TTATAA 1 cut(s) 1749
Psp5II RGGWCCY 3 cut(s) 1925, 2327, 3670
Psp6I CCWGG 1 cut(s) 1855
PspFI CCCAGC 1 cut(s) 3496
PspGI CCWGG 1 cut(s) 1855
PspN4I GGNNCC 3 cut(s) 1926, 2428, 2429
PspOMI GGGCCC 1 cut(s) 2427
PspPI GGNCC 8 cut(s) 413, 1925, 2327, 2427, 2428, 3251, 3670, 3803
PspPPI RGGWCCY 3 cut(s) 1925, 2327, 3670
PsrI GAACNNNNNNTAC 2 cut(s) 3838, 3870
PstI CTGCAG 1 cut(s) 3437
PstNI CAGNNNCTG 1 cut(s) 2437
PsuI RGATCY 5 cut(s) 100, 202, 259, 868, 3481
PvuII CAGCTG 2 cut(s) 112, 2500
RsaI GTAC 6 cut(s) 471, 2115, 2262, 2777, 2900, 3070
RsaNI GTAC 6 cut(s) 470, 2114, 2261, 2776, 2899, 3069
RseI CAYNNNNRTG 2 cut(s) 576, 684
SalI GTCGAC 1 cut(s) 155
SapI GCTCTTC 1 cut(s) 2461
Sau96I GGNCC 8 cut(s) 413, 1925, 2327, 2427, 2428, 3251, 3670, 3803
ScaI AGTACT 3 cut(s) 471, 2262, 2777
SchI GAGTC 3 cut(s) 1069, 2262, 3703
ScrFI CCNGG 3 cut(s) 621, 1857, 3051
SduI GDGCHC 3 cut(s) 2431, 2963, 3244
SfcI CTRYAG 2 cut(s) 3433, 3763
SinI GGWCC 3 cut(s) 1925, 2327, 3670
SmiMI CAYNNNNRTG 2 cut(s) 576, 684
SpeI ACTAGT 1 cut(s) 1129
SphI GCATGC 1 cut(s) 2596
SseBI AGGCCT 1 cut(s) 2994
SsiI CCGC 3 cut(s) 1822, 2628, 3361
SspI AATATT 3 cut(s) 39, 133, 1427
StuI AGGCCT 1 cut(s) 2994
StyD4I CCNGG 3 cut(s) 619, 1855, 3049
StyI CCWWGG 5 cut(s) 45, 1585, 1793, 2062, 3105
TaaI ACNGT 7 cut(s) 2351, 2360, 2921, 3068, 3429, 3679, 3767
TaiI ACGT 3 cut(s) 1467, 1621, 2876
TaqI TCGA 7 cut(s) 156, 172, 811, 1307, 3126, 3147, 3486
TatI WGTACW 5 cut(s) 469, 2113, 2260, 2775, 2898
TauI GCSGC 1 cut(s) 1825
TfiI GAWTC 9 cut(s) 119, 662, 1211, 1713, 2509, 2704, 2821, 3524, 3820
TscAI CASTG 1 cut(s) 2963
TseFI GTSAC 5 cut(s) 49, 567, 940, 953, 3423
Tsp45I GTSAC 5 cut(s) 49, 567, 940, 953, 3423
TspGWI ACGGA 2 cut(s) 2781, 3200
TspRI CASTG 1 cut(s) 2963
Van91I CCANNNNNTGG 3 cut(s) 2095, 2264, 3005
Vha464I CTTAAG 1 cut(s) 736
VpaK11BI GGWCC 3 cut(s) 1925, 2327, 3670
VspI ATTAAT 1 cut(s) 912
XbaI TCTAGA 3 cut(s) 2893, 3322, 3775
XceI RCATGY 3 cut(s) 1538, 2596, 3399
XmiI GTMKAC 3 cut(s) 156, 1123, 3400
XmnI GAANNNNTTC 6 cut(s) 311, 395, 981, 1237, 1788, 3573
ZrmI AGTACT 3 cut(s) 471, 2262, 2777
Zsp2I ATGCAT 1 cut(s) 3493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.