Rorug04G0317700

methyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0016641
Physical Location & Seq
Reverse (-)
49208252 .. 49209700
1449 bp
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UTR
Exon/CDS
Intron
Rorug04G0317700.1

Sequence Viewer

Length: 1449 bp
ATGGAGATAGTTGACAACGAAGATGTAGCTAGTTGGGTGTGTGACAGCCTCTCAGTGCAACCTAACAAGATTCCATTATACATTACTCTGGAACGCAGCTTATCAAGATCTGTAAGTGGAAGTAATCAAGGCATGAATAGTAGACCTAACCCATCTTTTCAGTATGATGATGTTGTCTCAACAATGTTCTCTACTGAGCTTGAAGATCAAAGTCCAATTGCATTGAGTTCCCCGCATGAGCCAATAGTAGTAAACTCTGGGTTGGATAATGGTGATAATGTCTCATACGAAAATAGAGAGGAAGATGAGTTTCCACATGCCAATTGGGATAATGAGAGTCCAATGGATGATGTAGAAATTGAAACTGAGCAACCTACGAATGCTAACTCTACTCCATCATCTTCTTATCTTAGCAAGAGAAGGCGCATAGAAGACTATGACAATGTTGCATTTGTGGACACCAATGAAGATATTTTGGTAGGTCAGTTATATCCGAGTAAGAAAGCCTTGAAACAACATTTGGGCATGCTCGCAATAAGAAAGAATTACGAGTTTAAAGTGAACAAATCAGCTAGTGACCGGTTTGAAGTTAGGTGTGCGGATCCTAACTGTAAGTGGCGACTCCGTGCAATAAAGCTACAAGACTTGGCCTATTTTGAAGTAAGAAAGTTTCATAATGAACATTCTTGTTCGTTGGATATTATTCATCGTGATCATCGACAAGCAAGCAGCTCCCTAATTGGTATCTTTGTCAAGTCAAAGTTTGAGGGAGGAACCTCGCGTATATACAGACCTAGAGATATCATTGATGATGCGCGTGTCCAACTTGGAGTTAATATGGGCTATAATAAAGCTTGGAGGGCAAGAGAAGCAGCACTAGAAATTGCAAGGGGATCACCAGAGGAATCATTTGCTATACTTCCAACATATTGTGCTATGTTAGAGCGCAAAAATCAAGGTACAATAACACACATAGAAACTGATGAACATAATCACTTTCTATACTTTTTCATGGCAATTGGAGCCTCAATTAGAGGATTTCGTGATTCTATGAGGCCCGTGATCGCTATAGATGGTACATTTTTGAAAGGAAAGTACTTTGGAACCATGTTTGTGGCTGCATGCCAAGATGGAGAGAATCAAATATATCCTTTGGCATTTGGGGTGGTAGACTCGGAAAATGATGCCTCATGGTCATGGTTTTTGACAAAATTGAGAGGGGCCATTGGAGTTGTAGAGAACTTGGTTTTTATATCTGATCGACATGGAAGCATTGCCAAAGCTGTGGATAATGTTTTTCCTGAAGCACATCATGGAGCCTGCATCTTTCATATAGCTAACAACCTGCAATCAAAGTTTGGCAAAAAAATGAAGATCCTCAAGTTATATTACACAGCAGCAAAGAAGTACCAAGTATCCGAGTTCAATACATTGATGGATGACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

482

Amino Acids

54.67

Weight (kDa)

5.6

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBD_Tnp_Mut PF03108 159 - 221 1.3e-11 MuDR family transposase
MULE PF10551 354 - 448 1.8e-25 MULE transposase domain
Transposase_mut PF00872 374 - 456 2e-10 Transposase, Mutator family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016641)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26761
fragaria_vesca FvH4_4g30980
malus_domestica MD16G1068900.v1.1
prunus_persica Prupe.1G278300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0439341
rosa_laevigata RLG00000006279
rosa_multiflora Rmu_ssc0000171.1_g000010
rosa_roxburghii Rroxscaffold_5G00380280
rosa_rugosa Rorug04G0317700
rosa_samantha Rh4AG368400 Rh4BG380400 Rh4CG394800 Rh4DG374700
rosa_wichuraiana Rw0G002750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1353
AccI GTMKAC 2 cut(s) 142, 1170
AccII CGCG 2 cut(s) 781, 817
AciI CCGC 2 cut(s) 233, 599
AclWI GGATC 4 cut(s) 596, 609, 901, 1369
AcuI CTGAAG 1 cut(s) 1323
AfaI GTAC 4 cut(s) 961, 1078, 1097, 1409
AgeI ACCGGT 1 cut(s) 579
AgsI TTSAA 7 cut(s) 203, 362, 511, 587, 659, 1087, 1426
AjuI GAANNNNNNNTTGG 2 cut(s) 503, 535
AluBI AGCT 9 cut(s) 29, 99, 199, 572, 637, 732, 854, 1283, 1337
AluI AGCT 9 cut(s) 29, 99, 199, 572, 637, 732, 854, 1283, 1337
Alw26I GTCTC 2 cut(s) 181, 286
AlwI GGATC 4 cut(s) 596, 609, 901, 1369
AoxI GGCC 3 cut(s) 648, 1055, 1221
ApeKI GCWGC 5 cut(s) 96, 729, 872, 1118, 1397
AsiGI ACCGGT 1 cut(s) 579
AspLEI GCGC 3 cut(s) 426, 817, 948
AspS9I GGNCC 2 cut(s) 1056, 1221
AsuHPI GGTGA 2 cut(s) 284, 888
BamHI GGATCC 1 cut(s) 601
BbsI GAAGAC 1 cut(s) 438
BbvI GCAGC 5 cut(s) 108, 741, 884, 1105, 1409
BccI CCATC 5 cut(s) 160, 403, 1067, 1124, 1429
BciVI GTATCC 1 cut(s) 1426
BclI TGATCA 1 cut(s) 712
BcoDI GTCTC 2 cut(s) 181, 286
BfaI CTAG 4 cut(s) 30, 573, 795, 878
BfmI CTRYAG 1 cut(s) 1068
BfuAI ACCTGC 1 cut(s) 1353
BfuI GTATCC 1 cut(s) 1426
BglII AGATCT 1 cut(s) 107
BisI GCNGC 5 cut(s) 97, 730, 873, 1119, 1398
BlsI GCNGC 5 cut(s) 98, 731, 874, 1120, 1399
BmcAI AGTACT 1 cut(s) 1097
BmgT120I GGNCC 2 cut(s) 1056, 1221
BmiI GGNNCC 6 cut(s) 603, 775, 1024, 1105, 1222, 1318
BmsI GCATC 3 cut(s) 802, 1174, 1332
BpiI GAAGAC 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 1364
BsaWI WCCGGW 1 cut(s) 579
Bse118I RCCGGY 1 cut(s) 579
Bse3DI GCAATG 1 cut(s) 1272
BseGI GGATG 2 cut(s) 352, 1444
BseMI GCAATG 1 cut(s) 1272
BseMII CTCAG 3 cut(s) 66, 186, 357
BseXI GCAGC 5 cut(s) 108, 741, 884, 1105, 1409
Bsh1236I CGCG 2 cut(s) 781, 817
BshFI GGCC 3 cut(s) 650, 1057, 1223
BshTI ACCGGT 1 cut(s) 579
BsiSI CCGG 1 cut(s) 580
BsmAI GTCTC 2 cut(s) 181, 286
BsmI GAATGC 1 cut(s) 385
BsnI GGCC 3 cut(s) 650, 1057, 1223
Bsp143I GATC 8 cut(s) 107, 205, 601, 712, 893, 1062, 1258, 1374
BspACI CCGC 2 cut(s) 233, 599
BspANI GGCC 3 cut(s) 650, 1057, 1223
BspCNI CTCAG 3 cut(s) 65, 187, 358
BspFNI CGCG 2 cut(s) 781, 817
BspLI GGNNCC 6 cut(s) 603, 775, 1024, 1105, 1222, 1318
BspMI ACCTGC 1 cut(s) 1353
BspPI GGATC 4 cut(s) 596, 609, 901, 1369
BsrDI GCAATG 1 cut(s) 1272
BsrFI RCCGGY 1 cut(s) 579
BssAI RCCGGY 1 cut(s) 579
BssMI GATC 8 cut(s) 107, 205, 601, 712, 893, 1062, 1258, 1374
Bst4CI ACNGT 1 cut(s) 611
BstC8I GCNNGC 5 cut(s) 527, 531, 727, 1123, 1321
BstDEI CTNAG 4 cut(s) 52, 195, 366, 410
BstF5I GGATG 2 cut(s) 352, 1444
BstFNI CGCG 2 cut(s) 781, 817
BstHHI GCGC 3 cut(s) 426, 817, 948
BstKTI GATC 8 cut(s) 110, 208, 604, 715, 896, 1065, 1261, 1377
BstMAI GTCTC 2 cut(s) 181, 286
BstMBI GATC 8 cut(s) 107, 205, 601, 712, 893, 1062, 1258, 1374
BstMWI GCNNNNNNNGC 3 cut(s) 860, 869, 1022
BstNSI RCATGY 3 cut(s) 320, 529, 1125
BstSFI CTRYAG 1 cut(s) 1068
BstUI CGCG 2 cut(s) 781, 817
BstV1I GCAGC 5 cut(s) 108, 741, 884, 1105, 1409
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 3 cut(s) 107, 601, 1374
BstXI CCANNNNNNTGG 2 cut(s) 1114, 1285
BstYI RGATCY 3 cut(s) 107, 601, 1374
BsuI GTATCC 1 cut(s) 1426
BsuRI GGCC 3 cut(s) 650, 1057, 1223
BtsCI GGATG 2 cut(s) 352, 1444
BtsIMutI CAGTG 1 cut(s) 60
BveI ACCTGC 1 cut(s) 1353
Cac8I GCNNGC 5 cut(s) 527, 531, 727, 1123, 1321
CfoI GCGC 3 cut(s) 426, 817, 948
Cfr10I RCCGGY 1 cut(s) 579
Cfr13I GGNCC 2 cut(s) 1056, 1221
Csp6I GTAC 4 cut(s) 960, 1077, 1096, 1408
CspAI ACCGGT 1 cut(s) 579
CviQI GTAC 4 cut(s) 960, 1077, 1096, 1408
DdeI CTNAG 4 cut(s) 52, 195, 366, 410
DpnI GATC 8 cut(s) 109, 207, 603, 714, 895, 1064, 1260, 1376
DpnII GATC 8 cut(s) 107, 205, 601, 712, 893, 1062, 1258, 1374
DraI TTTAAA 1 cut(s) 556
Eco32I GATATC 1 cut(s) 802
Eco57I CTGAAG 1 cut(s) 1323
EcoRV GATATC 1 cut(s) 802
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FauI CCCGC 1 cut(s) 240
FbaI TGATCA 1 cut(s) 712
FblI GTMKAC 2 cut(s) 142, 1170
Fnu4HI GCNGC 5 cut(s) 97, 730, 873, 1119, 1398
FokI GGATG 1 cut(s) 359
Fsp4HI GCNGC 5 cut(s) 97, 730, 873, 1119, 1398
FspBI CTAG 4 cut(s) 30, 573, 795, 878
GlaI GCGC 3 cut(s) 425, 816, 947
GluI GCNGC 5 cut(s) 97, 730, 873, 1119, 1398
HaeIII GGCC 3 cut(s) 650, 1057, 1223
HapII CCGG 1 cut(s) 580
HhaI GCGC 3 cut(s) 426, 817, 948
Hin6I GCGC 3 cut(s) 424, 815, 946
HinP1I GCGC 3 cut(s) 424, 815, 946
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HindIII AAGCTT 1 cut(s) 852
HinfI GANTC 7 cut(s) 70, 337, 621, 905, 1046, 1138, 1172
HpaII CCGG 1 cut(s) 580
HphI GGTGA 2 cut(s) 284, 888
Hpy166II GTNNAC 6 cut(s) 13, 143, 253, 457, 562, 1171
Hpy188I TCNGA 4 cut(s) 495, 1177, 1258, 1420
Hpy188III TCNNGA 5 cut(s) 89, 105, 710, 1043, 1301
Hpy8I GTNNAC 6 cut(s) 13, 143, 253, 457, 562, 1171
HpyAV CCTTC 1 cut(s) 414
HpyCH4III ACNGT 1 cut(s) 611
HpyCH4V TGCA 8 cut(s) 58, 221, 449, 629, 887, 1121, 1323, 1348
HpyF10VI GCNNNNNNNGC 3 cut(s) 860, 869, 1022
HpyF3I CTNAG 4 cut(s) 52, 195, 366, 410
HspAI GCGC 3 cut(s) 424, 815, 946
Ksp22I TGATCA 1 cut(s) 712
Kzo9I GATC 8 cut(s) 107, 205, 601, 712, 893, 1062, 1258, 1374
LmnI GCTCC 3 cut(s) 737, 1022, 1316
LpnPI CCDG 7 cut(s) 74, 243, 593, 912, 1314, 1333, 1358
Lsp1109I GCAGC 5 cut(s) 108, 741, 884, 1105, 1409
LweI GCATC 3 cut(s) 802, 1174, 1332
MaeI CTAG 4 cut(s) 30, 573, 795, 878
MaeIII GTNAC 2 cut(s) 41, 575
MalI GATC 8 cut(s) 109, 207, 603, 714, 895, 1064, 1260, 1376
MboI GATC 8 cut(s) 107, 205, 601, 712, 893, 1062, 1258, 1374
MboII GAAGA 7 cut(s) 32, 215, 314, 393, 443, 479, 1384
MfeI CAATTG 3 cut(s) 216, 322, 1017
MflI RGATCY 3 cut(s) 107, 601, 1374
MluCI AATT 9 cut(s) 216, 322, 357, 544, 738, 882, 1017, 1029, 1211
MlyI GAGTC 3 cut(s) 346, 615, 1166
MmeI TCCRAC 4 cut(s) 243, 675, 847, 947
MseI TTAA 2 cut(s) 555, 834
MslI CAYNNNNRTG 2 cut(s) 1112, 1195
MspI CCGG 1 cut(s) 580
MunI CAATTG 3 cut(s) 216, 322, 1017
Mva1269I GAATGC 1 cut(s) 385
MvnI CGCG 2 cut(s) 781, 817
MwoI GCNNNNNNNGC 3 cut(s) 860, 869, 1022
NdeII GATC 8 cut(s) 107, 205, 601, 712, 893, 1062, 1258, 1374
NlaIV GGNNCC 6 cut(s) 603, 775, 1024, 1105, 1222, 1318
NmuCI GTSAC 2 cut(s) 41, 575
NspI RCATGY 3 cut(s) 320, 529, 1125
PaeI GCATGC 2 cut(s) 529, 1125
PcsI WCGNNNNNNNCGW 1 cut(s) 715
PctI GAATGC 1 cut(s) 385
PfeI GAWTC 4 cut(s) 70, 905, 1046, 1138
PinAI ACCGGT 1 cut(s) 579
PkrI GCNGC 5 cut(s) 98, 731, 874, 1120, 1399
PleI GAGTC 3 cut(s) 345, 615, 1166
PpsI GAGTC 3 cut(s) 345, 615, 1166
PspN4I GGNNCC 6 cut(s) 603, 775, 1024, 1105, 1222, 1318
PspPI GGNCC 2 cut(s) 1056, 1221
PsuI RGATCY 3 cut(s) 107, 601, 1374
RsaI GTAC 4 cut(s) 961, 1078, 1097, 1409
RsaNI GTAC 4 cut(s) 960, 1077, 1096, 1408
RseI CAYNNNNRTG 2 cut(s) 1112, 1195
SaqAI TTAA 2 cut(s) 555, 834
SatI GCNGC 5 cut(s) 97, 730, 873, 1119, 1398
Sau3AI GATC 8 cut(s) 107, 205, 601, 712, 893, 1062, 1258, 1374
Sau96I GGNCC 2 cut(s) 1056, 1221
ScaI AGTACT 1 cut(s) 1097
SchI GAGTC 3 cut(s) 346, 615, 1166
SfaNI GCATC 3 cut(s) 802, 1174, 1332
SfcI CTRYAG 1 cut(s) 1068
SmiMI CAYNNNNRTG 2 cut(s) 1112, 1195
SmlI CTYRAG 1 cut(s) 1379
SmoI CTYRAG 1 cut(s) 1379
SphI GCATGC 2 cut(s) 529, 1125
Sse9I AATT 9 cut(s) 216, 322, 357, 544, 738, 882, 1017, 1029, 1211
SsiI CCGC 2 cut(s) 233, 599
SspMI CTAG 4 cut(s) 30, 573, 795, 878
TaaI ACNGT 1 cut(s) 611
TaqI TCGA 2 cut(s) 718, 1261
TasI AATT 9 cut(s) 216, 322, 357, 544, 738, 882, 1017, 1029, 1211
TatI WGTACW 1 cut(s) 1095
TfiI GAWTC 4 cut(s) 70, 905, 1046, 1138
Tru1I TTAA 2 cut(s) 555, 834
Tru9I TTAA 2 cut(s) 555, 834
TscAI CASTG 1 cut(s) 60
TseFI GTSAC 2 cut(s) 41, 575
TseI GCWGC 5 cut(s) 96, 729, 872, 1118, 1397
Tsp45I GTSAC 2 cut(s) 41, 575
TspDTI ATGAA 9 cut(s) 149, 480, 662, 693, 695, 999, 1000, 1319, 1385
TspGWI ACGGA 1 cut(s) 614
TspRI CASTG 1 cut(s) 60
XceI RCATGY 3 cut(s) 320, 529, 1125
XcmI CCANNNNNNNNNTGG 1 cut(s) 321
XmiI GTMKAC 2 cut(s) 142, 1170
XspI CTAG 4 cut(s) 30, 573, 795, 878
ZrmI AGTACT 1 cut(s) 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.