Rh4AG368400

methyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0016641
Physical Location & Seq
Forward (+)
67594420 .. 67596160
1741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG368400.1

Sequence Viewer

Length: 1410 bp
ATGGATACAGCTTCTGCTACTGCAATGAAAGCTCTCAACCTCCTTGCAACTCCAACGAAGCAAAGAGCAACGGCCTCAACTAACTTAACCTCTCAAACTCCACCCACTAAACCAAACTCACACACTCTTCATCTCCAAAGCTCAAACCCCAGAACCAGAGCTATCTTTCTCACTCGCTGCTCCACAAAGCCGGACACATACACTGACAACCAGGAAGACCAAAATTCGACCGTTGAGCCCAATTTAAATTCAAAACCCCTAAACCCTTCCCCGCTCACGTCAGTTGATCCACTTTCTTCTCCTATATCATCATTTCCTTCGTCTTCCACCAAAGGACTGGTGTTCGATTTGGGTGTGGAGAATTCATGGGACGGAGCAGGAGTTGGGTCCCCAGTCGTGAAGCGATTCCTCGGCGACGAGGAAGAGAGGTGGTACATGTGGTATAACGGGCGGTCCAATTCGAATCCGGGTTCGGATTCGATCGGGTTAGCGATTTCGAGCAATGGGGTTCACTGGAATCGAGGCAGAGGAGCGGTTCAGTCGAGCCAGGATGTTGGTTTGGTGATGAGTAGCGGCAAAGATTGGTGGGCGTTTGATACTTTAAGCATTAGGCCTTCTGAGGTTGTGGTAATGTCAAGCTCAAAGGTCAGAGCTTCTAGTGCTGTGTATTGGCTCTATTATACTGGTTATAGTCCCGAGAAGGTAGAGATTTCCGAGGTTCCTATCGGTTTGGAGAATCCGGAACAGTCCGGGCAGGTGTTCAAGTCTTTGCTTGGCTTGGCAATAAGCCAGGATGGGAGGCATTGGGCTAGAATTGAAGGGGAGCATCATAGTGGAGCTTTGTTTGATGTAGGGATGGAGAAGGATTGGGATTCTTCATTCATTGCTGGGTCTCATGTTGTGTTTCATGAGAGAGGGGATCTTAGAATGTATTACCATTCATTTGATTTGGAGAATGGGCACTATGGCATTGGGATTGCAAGGTCTAGGGATGGGATGAAGTGGGTCAAGTTGGGGAAGATAATTGGGGGAGGGAGGAGTGGTGGGTTTGATGAGTTGGGGGCAATGAATCCTTGTGTGGTTAGGAAAAGGGGAAGTGGCGAATATTTGATGGCATATGAAGGTGTTGTTGGGAATGGAGGGAGGAGTATTGGGTTGGCAGTTTCAAGAGATGGGTTGAAGGATTGGACAAGGTGTGGAGATGAGGCCGTGCTGAAGTCATCTGAGAAATCAGGATGGGATAGCAATGGAGTTGGGTCACCATGCTTGGTTCAAATGGATGGGGAAGAAGATGAGTGGAGGTTGTATTATAGAGGTGTTGGGATTGGGGGGAGGACAGGGATTGGAATGGCTGTTTCAGAAGGGAGTGATTATAGAAGTTTTAGAAGATGGGCAGGATTTCATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

469

Amino Acids

51.1

Weight (kDa)

6.15

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016641)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26761
fragaria_vesca FvH4_4g30980
malus_domestica MD16G1068900.v1.1
prunus_persica Prupe.1G278300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0439341
rosa_laevigata RLG00000006279
rosa_multiflora Rmu_ssc0000171.1_g000010
rosa_roxburghii Rroxscaffold_5G00380280
rosa_rugosa Rorug04G0317700
rosa_samantha Rh4AG368400 Rh4BG380400 Rh4CG394800 Rh4DG374700
rosa_wichuraiana Rw0G002750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 745
Acc36I ACCTGC 1 cut(s) 745
AccBSI CCGCTC 2 cut(s) 274, 533
AccIII TCCGGA 1 cut(s) 739
AciI CCGC 4 cut(s) 272, 451, 533, 573
AclWI GGATC 2 cut(s) 281, 927
AcsI RAATTY 3 cut(s) 223, 247, 361
AcuI CTGAAG 1 cut(s) 1235
AfaI GTAC 1 cut(s) 434
AflIII ACRYGT 1 cut(s) 435
AgsI TTSAA 6 cut(s) 252, 763, 818, 1167, 1180, 1274
AjiI CACGTC 1 cut(s) 279
AjnI CCWGG 3 cut(s) 210, 546, 789
AjuI GAANNNNNNNTTGG 2 cut(s) 1113, 1145
AluBI AGCT 7 cut(s) 11, 32, 141, 161, 639, 653, 839
AluI AGCT 7 cut(s) 11, 32, 141, 161, 639, 653, 839
Alw26I GTCTC 1 cut(s) 897
AlwI GGATC 2 cut(s) 281, 927
AlwNI CAGNNNCTG 1 cut(s) 14
Ama87I CYCGRG 1 cut(s) 695
Aor13HI TCCGGA 1 cut(s) 739
AoxI GGCC 3 cut(s) 72, 611, 1206
ApeKI GCWGC 1 cut(s) 177
ApoI RAATTY 3 cut(s) 223, 247, 361
Asp700I GAANNNNTTC 1 cut(s) 404
AspS9I GGNCC 2 cut(s) 387, 453
AsuC2I CCSGG 2 cut(s) 468, 751
AsuHPI GGTGA 2 cut(s) 574, 1251
AsuII TTCGAA 1 cut(s) 461
AvaI CYCGRG 1 cut(s) 695
AvaII GGWCC 2 cut(s) 387, 453
BaeGI GKGCMC 1 cut(s) 963
BanII GRGCYC 1 cut(s) 240
BbsI GAAGAC 2 cut(s) 222, 315
BbvI GCAGC 1 cut(s) 164
BccI CCATC 8 cut(s) 788, 850, 986, 1105, 1166, 1230, 1274, 1383
BceAI ACGGC 2 cut(s) 87, 1193
BciT130I CCWGG 3 cut(s) 212, 548, 791
BcnI CCSGG 2 cut(s) 468, 751
BcoDI GTCTC 1 cut(s) 897
BfaI CTAG 3 cut(s) 657, 810, 987
BfuAI ACCTGC 1 cut(s) 745
BisI GCNGC 2 cut(s) 178, 574
BlsI GCNGC 2 cut(s) 179, 575
Bme1390I CCNGG 5 cut(s) 212, 468, 548, 751, 791
Bme18I GGWCC 2 cut(s) 387, 453
BmeT110I CYCGRG 1 cut(s) 695
BmgBI CACGTC 1 cut(s) 279
BmgT120I GGNCC 2 cut(s) 387, 453
BmiI GGNNCC 3 cut(s) 388, 389, 720
BmrFI CCNGG 5 cut(s) 212, 468, 548, 751, 791
BmrI ACTGGG 1 cut(s) 386
BmsI GCATC 1 cut(s) 835
BmuI ACTGGG 1 cut(s) 386
BpiI GAAGAC 2 cut(s) 222, 315
Bpu14I TTCGAA 1 cut(s) 461
BpuMI CCSGG 2 cut(s) 468, 751
BsaI GGTCTC 1 cut(s) 897
BsaJI CCNNGG 2 cut(s) 409, 714
BsaWI WCCGGW 1 cut(s) 739
BsaXI ACNNNNNCTCC 4 cut(s) 366, 396, 1027, 1057
Bse1I ACTGG 4 cut(s) 342, 392, 518, 688
Bse3DI GCAATG 5 cut(s) 30, 508, 882, 1071, 1252
BseAI TCCGGA 1 cut(s) 739
BseBI CCWGG 3 cut(s) 212, 548, 791
BseDI CCNNGG 2 cut(s) 409, 714
BseGI GGATG 7 cut(s) 556, 799, 861, 997, 1002, 1241, 1285
BseMI GCAATG 5 cut(s) 30, 508, 882, 1071, 1252
BseMII CTCAG 2 cut(s) 609, 1215
BseNI ACTGG 4 cut(s) 342, 392, 518, 688
BseRI GAGGAG 3 cut(s) 543, 1051, 1159
BseSI GKGCMC 1 cut(s) 963
BseXI GCAGC 1 cut(s) 164
BseYI CCCAGC 1 cut(s) 887
Bsh1285I CGRYCG 2 cut(s) 231, 483
BshFI GGCC 3 cut(s) 74, 613, 1208
BsiEI CGRYCG 2 cut(s) 231, 483
BsiHKCI CYCGRG 1 cut(s) 695
BsiSI CCGG 4 cut(s) 191, 467, 740, 750
BslFI GGGAC 3 cut(s) 373, 383, 678
BsmAI GTCTC 1 cut(s) 897
BsmFI GGGAC 3 cut(s) 373, 383, 678
BsnI GGCC 3 cut(s) 74, 613, 1208
Bso31I GGTCTC 1 cut(s) 897
BsoBI CYCGRG 1 cut(s) 695
Bsp119I TTCGAA 1 cut(s) 461
Bsp1286I GDGCHC 2 cut(s) 240, 963
Bsp13I TCCGGA 1 cut(s) 739
Bsp143I GATC 3 cut(s) 286, 480, 919
BspACI CCGC 4 cut(s) 272, 451, 533, 573
BspANI GGCC 3 cut(s) 74, 613, 1208
BspCNI CTCAG 2 cut(s) 610, 1216
BspEI TCCGGA 1 cut(s) 739
BspHI TCATGA 1 cut(s) 907
BspLI GGNNCC 3 cut(s) 388, 389, 720
BspMI ACCTGC 1 cut(s) 745
BspPI GGATC 2 cut(s) 281, 927
BspT104I TTCGAA 1 cut(s) 461
BspTNI GGTCTC 1 cut(s) 897
BsrBI CCGCTC 2 cut(s) 274, 533
BsrDI GCAATG 5 cut(s) 30, 508, 882, 1071, 1252
BsrI ACTGG 4 cut(s) 342, 392, 518, 688
BssECI CCNNGG 2 cut(s) 409, 714
BssMI GATC 3 cut(s) 286, 480, 919
Bst2UI CCWGG 3 cut(s) 212, 548, 791
Bst4CI ACNGT 2 cut(s) 232, 747
Bst6I CTCTTC 2 cut(s) 132, 417
BstBI TTCGAA 1 cut(s) 461
BstDEI CTNAG 3 cut(s) 618, 923, 1224
BstEII GGTNACC 1 cut(s) 1257
BstF5I GGATG 7 cut(s) 556, 799, 861, 997, 1002, 1241, 1285
BstKTI GATC 3 cut(s) 289, 483, 922
BstMAI GTCTC 1 cut(s) 897
BstMBI GATC 3 cut(s) 286, 480, 919
BstMCI CGRYCG 2 cut(s) 231, 483
BstMWI GCNNNNNNNGC 2 cut(s) 29, 659
BstNI CCWGG 3 cut(s) 212, 548, 791
BstNSI RCATGY 1 cut(s) 439
BstPI GGTNACC 1 cut(s) 1257
BstSCI CCNGG 5 cut(s) 210, 466, 546, 749, 789
BstSLI GKGCMC 1 cut(s) 963
BstV1I GCAGC 1 cut(s) 164
BstV2I GAAGAC 2 cut(s) 222, 315
BstX2I RGATCY 1 cut(s) 919
BstXI CCANNNNNNTGG 2 cut(s) 337, 554
BstYI RGATCY 1 cut(s) 919
BsuRI GGCC 3 cut(s) 74, 613, 1208
BtrI CACGTC 1 cut(s) 279
BtsCI GGATG 7 cut(s) 556, 799, 861, 997, 1002, 1241, 1285
BtsIMutI CAGTG 2 cut(s) 201, 511
BveI ACCTGC 1 cut(s) 745
CaiI CAGNNNCTG 1 cut(s) 14
CciI TCATGA 1 cut(s) 907
Cfr13I GGNCC 2 cut(s) 387, 453
Csp6I GTAC 1 cut(s) 433
CviAII CATG 5 cut(s) 366, 436, 896, 908, 1263
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 3 cut(s) 618, 923, 1224
DpnI GATC 3 cut(s) 288, 482, 921
DpnII GATC 3 cut(s) 286, 480, 919
DraI TTTAAA 1 cut(s) 246
Eam1104I CTCTTC 2 cut(s) 132, 417
EarI CTCTTC 2 cut(s) 132, 417
Eco147I AGGCCT 1 cut(s) 613
Eco24I GRGCYC 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 897
Eco47I GGWCC 2 cut(s) 387, 453
Eco57I CTGAAG 1 cut(s) 1235
Eco88I CYCGRG 1 cut(s) 695
Eco91I GGTNACC 1 cut(s) 1257
EcoO109I RGGNCCY 1 cut(s) 387
EcoO65I GGTNACC 1 cut(s) 1257
EcoRI GAATTC 1 cut(s) 361
EcoRII CCWGG 3 cut(s) 210, 546, 789
EcoT38I GRGCYC 1 cut(s) 240
FaeI CATG 5 cut(s) 369, 439, 899, 911, 1266
FaqI GGGAC 3 cut(s) 373, 383, 678
FatI CATG 5 cut(s) 365, 435, 895, 907, 1262
FauI CCCGC 1 cut(s) 279
FauNDI CATATG 1 cut(s) 1117
Fnu4HI GCNGC 2 cut(s) 178, 574
FokI GGATG 7 cut(s) 563, 806, 868, 1004, 1009, 1248, 1292
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 2 cut(s) 178, 574
FspBI CTAG 3 cut(s) 657, 810, 987
GluI GCNGC 2 cut(s) 178, 574
GsaI CCCAGC 1 cut(s) 891
HaeIII GGCC 3 cut(s) 74, 613, 1208
HapII CCGG 4 cut(s) 191, 467, 740, 750
Hin1II CATG 5 cut(s) 369, 439, 899, 911, 1266
HinfI GANTC 7 cut(s) 405, 463, 476, 517, 736, 872, 1069
HpaII CCGG 4 cut(s) 191, 467, 740, 750
HphI GGTGA 2 cut(s) 574, 1251
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 6 cut(s) 475, 619, 650, 715, 1225, 1360
Hpy188III TCNNGA 6 cut(s) 397, 695, 740, 908, 1167, 1233
Hpy8I GTNNAC 1 cut(s) 511
Hpy99I CGWCG 1 cut(s) 419
HpyAV CCTTC 9 cut(s) 276, 327, 624, 694, 812, 856, 1115, 1174, 1355
HpyCH4III ACNGT 2 cut(s) 232, 747
HpyCH4IV ACGT 1 cut(s) 278
HpyCH4V TGCA 3 cut(s) 23, 47, 980
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 659
HpyF3I CTNAG 3 cut(s) 618, 923, 1224
HpySE526I ACGT 1 cut(s) 278
Hsp92II CATG 5 cut(s) 369, 439, 899, 911, 1266
KflI GGGWCCC 1 cut(s) 387
Kpn2I TCCGGA 1 cut(s) 739
Kzo9I GATC 3 cut(s) 286, 480, 919
LmnI GCTCC 5 cut(s) 185, 374, 530, 823, 836
Lsp1109I GCAGC 1 cut(s) 164
LweI GCATC 1 cut(s) 835
MaeI CTAG 3 cut(s) 657, 810, 987
MaeII ACGT 1 cut(s) 278
MaeIII GTNAC 1 cut(s) 1257
MalI GATC 3 cut(s) 288, 482, 921
MbiI CCGCTC 2 cut(s) 274, 533
MboI GATC 3 cut(s) 286, 480, 919
MflI RGATCY 1 cut(s) 919
MhlI GDGCHC 2 cut(s) 240, 963
MluCI AATT 7 cut(s) 223, 241, 247, 361, 457, 813, 1023
MmeI TCCRAC 1 cut(s) 77
MroI TCCGGA 1 cut(s) 739
MroXI GAANNNNTTC 1 cut(s) 404
MseI TTAA 3 cut(s) 86, 245, 602
MslI CAYNNNNRTG 1 cut(s) 831
MspI CCGG 4 cut(s) 191, 467, 740, 750
MspR9I CCNGG 5 cut(s) 212, 468, 548, 751, 791
MvaI CCWGG 3 cut(s) 212, 548, 791
MwoI GCNNNNNNNGC 2 cut(s) 29, 659
NciI CCSGG 2 cut(s) 468, 751
NdeI CATATG 1 cut(s) 1117
NdeII GATC 3 cut(s) 286, 480, 919
NlaIII CATG 5 cut(s) 369, 439, 899, 911, 1266
NlaIV GGNNCC 3 cut(s) 388, 389, 720
NmeAIII GCCGAG 1 cut(s) 390
NmuCI GTSAC 1 cut(s) 1257
NspI RCATGY 1 cut(s) 439
NspV TTCGAA 1 cut(s) 461
PagI TCATGA 1 cut(s) 907
PaqCI CACCTGC 1 cut(s) 745
PceI AGGCCT 1 cut(s) 613
PciI ACATGT 1 cut(s) 435
PdmI GAANNNNTTC 1 cut(s) 404
PfeI GAWTC 7 cut(s) 405, 463, 476, 517, 736, 872, 1069
PkrI GCNGC 2 cut(s) 179, 575
Ple19I CGATCG 1 cut(s) 483
PpuMI RGGWCCY 1 cut(s) 387
PscI ACATGT 1 cut(s) 435
Psp5II RGGWCCY 1 cut(s) 387
Psp6I CCWGG 3 cut(s) 210, 546, 789
PspEI GGTNACC 1 cut(s) 1257
PspFI CCCAGC 1 cut(s) 887
PspGI CCWGG 3 cut(s) 210, 546, 789
PspN4I GGNNCC 3 cut(s) 388, 389, 720
PspPI GGNCC 2 cut(s) 387, 453
PspPPI RGGWCCY 1 cut(s) 387
PstNI CAGNNNCTG 1 cut(s) 14
PsuI RGATCY 1 cut(s) 919
PvuI CGATCG 1 cut(s) 483
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 1 cut(s) 831
SaqAI TTAA 3 cut(s) 86, 245, 602
SatI GCNGC 2 cut(s) 178, 574
Sau3AI GATC 3 cut(s) 286, 480, 919
Sau96I GGNCC 2 cut(s) 387, 453
ScrFI CCNGG 5 cut(s) 212, 468, 548, 751, 791
SduI GDGCHC 2 cut(s) 240, 963
SfaNI GCATC 1 cut(s) 835
SfuI TTCGAA 1 cut(s) 461
SinI GGWCC 2 cut(s) 387, 453
SmiI ATTTAAAT 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 831
Sse9I AATT 7 cut(s) 223, 241, 247, 361, 457, 813, 1023
SseBI AGGCCT 1 cut(s) 613
SsiI CCGC 4 cut(s) 272, 451, 533, 573
SspI AATATT 1 cut(s) 1106
SspMI CTAG 3 cut(s) 657, 810, 987
StuI AGGCCT 1 cut(s) 613
StyD4I CCNGG 5 cut(s) 210, 466, 546, 749, 789
SwaI ATTTAAAT 1 cut(s) 246
TaaI ACNGT 2 cut(s) 232, 747
TaiI ACGT 1 cut(s) 281
TaqI TCGA 7 cut(s) 227, 345, 461, 479, 497, 520, 542
TasI AATT 7 cut(s) 223, 241, 247, 361, 457, 813, 1023
TauI GCSGC 1 cut(s) 576
TfiI GAWTC 7 cut(s) 405, 463, 476, 517, 736, 872, 1069
Tru1I TTAA 3 cut(s) 86, 245, 602
Tru9I TTAA 3 cut(s) 86, 245, 602
TscAI CASTG 2 cut(s) 208, 518
TseFI GTSAC 1 cut(s) 1257
TseI GCWGC 1 cut(s) 177
Tsp45I GTSAC 1 cut(s) 1257
TspGWI ACGGA 1 cut(s) 387
TspRI CASTG 2 cut(s) 208, 518
VpaK11BI GGWCC 2 cut(s) 387, 453
XapI RAATTY 3 cut(s) 223, 247, 361
XceI RCATGY 1 cut(s) 439
XcmI CCANNNNNNNNNTGG 1 cut(s) 334
XmnI GAANNNNTTC 1 cut(s) 404
XspI CTAG 3 cut(s) 657, 810, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.