Rorug04G0360700

protein NRT1 PTR FAMILY 2.13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009995
Physical Location & Seq
Reverse (-)
53328148 .. 53329122
975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0360700.1

Sequence Viewer

Length: 753 bp
ATGAACTCAGTGAAGACGTTCCATGAAAATGAACAGCGTTCAGTTGCAAGGAAGGAGACGATGATCAGTGGCAACCGACCCTCTCCATTGAAGATCAACAGAGATTCTTATCTCATTCATAAACAACAGCCTTCTCGCATGGCCGGAGCTCATGGATATCATCAACAGCAACAACAGCGGCACCATCCGGTCATCATTTACACTCAGTCTCCAAAAGTCATCCACACACGACCCCACGATTTCATGGCTTTGGTCCAAAAGCTCACCGGCCTCTCACGGTCCGATGAAGAACACGTCGTTAAAGAAGACCAAACTCATAATGAAGCTGCTCACAAAACCCTACATGCTCGTCAAGTTGAGGATAGTACCATCATCCACAGGTGTATTAATGATAATGAAACCTCTTCCGGTGTGACAGATGAAATTGGTGGTGGTGGTGATGATCATCGTCATGGGCAGAATATTAATGTTGGCGGTGATGTTCGAGCTGACAGTTCCTGTTTTAAAGCATCATCAGCAGGTCCAAATCGCACTTTAAGTGGCTTTAGCCCAATGCAGCAGCAAAATCAGCATCCATATTTTGCAGATATTCCAATGTTTACGCCGACTTCGATGGATCATCATCACTTGCTCTGCTCACCTCGCCCGCTTTACAGATTTCCGGATTCGAGCTCACAGATCTCTCCGAGTACTCTTTGCGGTTCAATCTCTCCTTCAATGTTCAAGTTCATCAAGGGACTACCAGAATACTAA

Protein Analysis

250

Amino Acids

28.08

Weight (kDa)

7.85

Isoelectric Point (pI)

56.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 70 - 92 1.1e-10 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009995)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27080 AT1G27080 AT1G69870
fragaria_vesca FvH4_4g35800 FvH4_4g35800
malus_domestica MD13G1080300.v1.1 MD16G1080100.v1.1
prunus_persica Prupe.1G266400_v2.0.a1
pyrus_communis pycom13g06950 pycom16g06920
rosa_chinensis RchiOBHm_Chr4g0445331
rosa_laevigata RLG00000005748
rosa_multiflora Rmu_sc0000050.1_g000012
rosa_roxburghii Rroxscaffold_5G00385850
rosa_rugosa Rorug04G0360700 Rorug04G0360800
rosa_samantha Rh4AG421800 Rh4BG433800 Rh4CG448700 Rh4DG429800
rosa_wichuraiana Rw4G036180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 509
AccB1I GGYRCC 1 cut(s) 180
AccIII TCCGGA 1 cut(s) 661
AciI CCGC 4 cut(s) 178, 474, 647, 699
AclWI GGATC 1 cut(s) 624
AcoI YGGCCR 1 cut(s) 141
AfaI GTAC 2 cut(s) 367, 691
AflIII ACRYGT 1 cut(s) 292
AgsI TTSAA 4 cut(s) 91, 705, 717, 724
AjiI CACGTC 1 cut(s) 295
AluBI AGCT 5 cut(s) 149, 262, 326, 488, 672
AluI AGCT 5 cut(s) 149, 262, 326, 488, 672
Alw21I GWGCWC 2 cut(s) 151, 674
Alw26I GTCTC 2 cut(s) 50, 213
AlwI GGATC 1 cut(s) 624
AlwNI CAGNNNCTG 1 cut(s) 498
Aor13HI TCCGGA 1 cut(s) 661
AoxI GGCC 2 cut(s) 141, 268
ApeKI GCWGC 3 cut(s) 326, 556, 559
AseI ATTAAT 2 cut(s) 387, 465
Asp700I GAANNNNTTC 1 cut(s) 17
AspS9I GGNCC 3 cut(s) 253, 279, 521
AsuHPI GGTGA 4 cut(s) 256, 449, 488, 630
AvaII GGWCC 3 cut(s) 253, 279, 521
BanI GGYRCC 1 cut(s) 180
BanII GRGCYC 2 cut(s) 151, 674
BbsI GAAGAC 2 cut(s) 20, 312
Bbv12I GWGCWC 2 cut(s) 151, 674
BbvI GCAGC 3 cut(s) 313, 568, 571
BccI CCATC 3 cut(s) 192, 377, 607
BclI TGATCA 2 cut(s) 63, 442
BcoDI GTCTC 2 cut(s) 50, 213
BfuAI ACCTGC 1 cut(s) 509
BglII AGATCT 1 cut(s) 678
BisI GCNGC 4 cut(s) 179, 327, 557, 560
BlsI GCNGC 4 cut(s) 180, 328, 558, 561
BmcAI AGTACT 1 cut(s) 691
Bme18I GGWCC 3 cut(s) 253, 279, 521
BmgBI CACGTC 1 cut(s) 295
BmgT120I GGNCC 3 cut(s) 253, 279, 521
BmiI GGNNCC 1 cut(s) 182
BmsI GCATC 2 cut(s) 518, 580
BpiI GAAGAC 2 cut(s) 20, 312
BsaBI GATNNNNATC 3 cut(s) 108, 444, 621
BsaWI WCCGGW 3 cut(s) 187, 407, 661
BsaXI ACNNNNNCTCC 2 cut(s) 193, 223
Bse118I RCCGGY 1 cut(s) 266
Bse8I GATNNNNATC 3 cut(s) 108, 444, 621
BseAI TCCGGA 1 cut(s) 661
BseGI GGATG 4 cut(s) 184, 219, 372, 571
BseJI GATNNNNATC 3 cut(s) 108, 444, 621
BseMII CTCAG 2 cut(s) 21, 218
BseXI GCAGC 3 cut(s) 313, 568, 571
BshFI GGCC 2 cut(s) 143, 270
BshNI GGYRCC 1 cut(s) 180
BsiHKAI GWGCWC 2 cut(s) 151, 674
BsiSI CCGG 5 cut(s) 144, 188, 267, 408, 662
BsmAI GTCTC 2 cut(s) 50, 213
BsmBI CGTCTC 1 cut(s) 50
BsnI GGCC 2 cut(s) 143, 270
Bsp1286I GDGCHC 2 cut(s) 151, 674
Bsp13I TCCGGA 1 cut(s) 661
Bsp143I GATC 5 cut(s) 63, 93, 442, 616, 678
BspACI CCGC 4 cut(s) 178, 474, 647, 699
BspANI GGCC 2 cut(s) 143, 270
BspCNI CTCAG 2 cut(s) 20, 217
BspEI TCCGGA 1 cut(s) 661
BspLI GGNNCC 1 cut(s) 182
BspMI ACCTGC 1 cut(s) 509
BspPI GGATC 1 cut(s) 624
BspT107I GGYRCC 1 cut(s) 180
BsrFI RCCGGY 1 cut(s) 266
BssAI RCCGGY 1 cut(s) 266
BssMI GATC 5 cut(s) 63, 93, 442, 616, 678
Bst4CI ACNGT 2 cut(s) 279, 494
Bst6I CTCTTC 1 cut(s) 409
BstC8I GCNNGC 1 cut(s) 647
BstDEI CTNAG 2 cut(s) 7, 204
BstF5I GGATG 4 cut(s) 184, 219, 372, 571
BstKTI GATC 5 cut(s) 66, 96, 445, 619, 681
BstMAI GTCTC 2 cut(s) 50, 213
BstMBI GATC 5 cut(s) 63, 93, 442, 616, 678
BstMWI GCNNNNNNNGC 4 cut(s) 175, 515, 568, 642
BstNSI RCATGY 1 cut(s) 347
BstV1I GCAGC 3 cut(s) 313, 568, 571
BstV2I GAAGAC 2 cut(s) 20, 312
BstX2I RGATCY 1 cut(s) 678
BstYI RGATCY 1 cut(s) 678
BsuRI GGCC 2 cut(s) 143, 270
BtrI CACGTC 1 cut(s) 295
BtsCI GGATG 4 cut(s) 184, 219, 372, 571
BtsIMutI CAGTG 2 cut(s) 15, 73
BveI ACCTGC 1 cut(s) 509
Cac8I GCNNGC 1 cut(s) 647
CaiI CAGNNNCTG 1 cut(s) 498
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 3 cut(s) 253, 279, 521
CpoI CGGWCCG 1 cut(s) 279
Csp6I GTAC 2 cut(s) 366, 690
CspI CGGWCCG 1 cut(s) 279
CviAII CATG 6 cut(s) 23, 139, 152, 244, 344, 452
CviQI GTAC 2 cut(s) 366, 690
DdeI CTNAG 2 cut(s) 7, 204
DpnI GATC 5 cut(s) 65, 95, 444, 618, 680
DpnII GATC 5 cut(s) 63, 93, 442, 616, 678
DraI TTTAAA 1 cut(s) 505
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 1 cut(s) 409
EarI CTCTTC 1 cut(s) 409
Ecl136II GAGCTC 2 cut(s) 149, 672
Eco24I GRGCYC 2 cut(s) 151, 674
Eco32I GATATC 1 cut(s) 158
Eco47I GGWCC 3 cut(s) 253, 279, 521
Eco53kI GAGCTC 2 cut(s) 149, 672
EcoICRI GAGCTC 2 cut(s) 149, 672
EcoRV GATATC 1 cut(s) 158
EcoT38I GRGCYC 2 cut(s) 151, 674
Esp3I CGTCTC 1 cut(s) 50
FaeI CATG 6 cut(s) 26, 142, 155, 247, 347, 455
FaiI YATR 9 cut(s) 24, 120, 140, 153, 245, 318, 345, 453, 577
FatI CATG 6 cut(s) 22, 138, 151, 243, 343, 451
FauI CCCGC 1 cut(s) 654
FbaI TGATCA 2 cut(s) 63, 442
Fnu4HI GCNGC 4 cut(s) 179, 327, 557, 560
FokI GGATG 4 cut(s) 171, 206, 359, 558
FriOI GRGCYC 2 cut(s) 151, 674
Fsp4HI GCNGC 4 cut(s) 179, 327, 557, 560
GluI GCNGC 4 cut(s) 179, 327, 557, 560
HaeIII GGCC 2 cut(s) 143, 270
HapII CCGG 5 cut(s) 144, 188, 267, 408, 662
Hin1II CATG 6 cut(s) 26, 142, 155, 247, 347, 455
HinfI GANTC 2 cut(s) 104, 665
HpaII CCGG 5 cut(s) 144, 188, 267, 408, 662
HphI GGTGA 4 cut(s) 256, 449, 488, 630
Hpy166II GTNNAC 1 cut(s) 600
Hpy188I TCNGA 2 cut(s) 283, 687
Hpy188III TCNNGA 1 cut(s) 662
Hpy8I GTNNAC 1 cut(s) 600
Hpy99I CGWCG 1 cut(s) 299
HpyAV CCTTC 3 cut(s) 46, 141, 723
HpyCH4III ACNGT 2 cut(s) 279, 494
HpyCH4IV ACGT 2 cut(s) 17, 294
HpyCH4V TGCA 3 cut(s) 47, 556, 584
HpyF10VI GCNNNNNNNGC 4 cut(s) 175, 515, 568, 642
HpyF3I CTNAG 2 cut(s) 7, 204
HpySE526I ACGT 2 cut(s) 17, 294
Hsp92II CATG 6 cut(s) 26, 142, 155, 247, 347, 455
Kpn2I TCCGGA 1 cut(s) 661
Ksp22I TGATCA 2 cut(s) 63, 442
Kzo9I GATC 5 cut(s) 63, 93, 442, 616, 678
LmnI GCTCC 1 cut(s) 146
LpnPI CCDG 8 cut(s) 157, 201, 280, 364, 421, 504, 511, 675
Lsp1109I GCAGC 3 cut(s) 313, 568, 571
LweI GCATC 2 cut(s) 518, 580
MaeII ACGT 2 cut(s) 17, 294
MaeIII GTNAC 1 cut(s) 412
MalI GATC 5 cut(s) 65, 95, 444, 618, 680
MboI GATC 5 cut(s) 63, 93, 442, 616, 678
MboII GAAGA 5 cut(s) 25, 103, 299, 317, 396
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 2 cut(s) 151, 674
MluCI AATT 1 cut(s) 423
MnlI CCTC 5 cut(s) 91, 281, 352, 412, 651
MroI TCCGGA 1 cut(s) 661
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 5 cut(s) 300, 387, 465, 504, 536
MslI CAYNNNNRTG 2 cut(s) 27, 450
MspA1I CMGCKG 1 cut(s) 178
MspI CCGG 5 cut(s) 144, 188, 267, 408, 662
MwoI GCNNNNNNNGC 4 cut(s) 175, 515, 568, 642
NdeII GATC 5 cut(s) 63, 93, 442, 616, 678
NlaIII CATG 6 cut(s) 26, 142, 155, 247, 347, 455
NlaIV GGNNCC 1 cut(s) 182
NmuCI GTSAC 1 cut(s) 412
NspI RCATGY 1 cut(s) 347
PcsI WCGNNNNNNNCGW 1 cut(s) 608
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 2 cut(s) 104, 665
PkrI GCNGC 4 cut(s) 180, 328, 558, 561
PshBI ATTAAT 2 cut(s) 387, 465
Psp124BI GAGCTC 2 cut(s) 151, 674
PspN4I GGNNCC 1 cut(s) 182
PspPI GGNCC 3 cut(s) 253, 279, 521
PstNI CAGNNNCTG 1 cut(s) 498
PsuI RGATCY 1 cut(s) 678
RsaI GTAC 2 cut(s) 367, 691
RsaNI GTAC 2 cut(s) 366, 690
RseI CAYNNNNRTG 2 cut(s) 27, 450
Rsr2I CGGWCCG 1 cut(s) 279
RsrII CGGWCCG 1 cut(s) 279
SacI GAGCTC 2 cut(s) 151, 674
SaqAI TTAA 5 cut(s) 300, 387, 465, 504, 536
SatI GCNGC 4 cut(s) 179, 327, 557, 560
Sau3AI GATC 5 cut(s) 63, 93, 442, 616, 678
Sau96I GGNCC 3 cut(s) 253, 279, 521
ScaI AGTACT 1 cut(s) 691
SduI GDGCHC 2 cut(s) 151, 674
SfaNI GCATC 2 cut(s) 518, 580
SinI GGWCC 3 cut(s) 253, 279, 521
SmiMI CAYNNNNRTG 2 cut(s) 27, 450
Sse9I AATT 1 cut(s) 423
SsiI CCGC 4 cut(s) 178, 474, 647, 699
SspI AATATT 1 cut(s) 463
SstI GAGCTC 2 cut(s) 151, 674
TaaI ACNGT 2 cut(s) 279, 494
TaiI ACGT 2 cut(s) 20, 297
TaqI TCGA 3 cut(s) 484, 611, 668
TasI AATT 1 cut(s) 423
TatI WGTACW 1 cut(s) 689
TauI GCSGC 1 cut(s) 181
TfiI GAWTC 2 cut(s) 104, 665
Tru1I TTAA 5 cut(s) 300, 387, 465, 504, 536
Tru9I TTAA 5 cut(s) 300, 387, 465, 504, 536
TscAI CASTG 2 cut(s) 15, 73
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 3 cut(s) 326, 556, 559
Tsp45I GTSAC 1 cut(s) 412
TspRI CASTG 2 cut(s) 15, 73
VpaK11BI GGWCC 3 cut(s) 253, 279, 521
VspI ATTAAT 2 cut(s) 387, 465
XceI RCATGY 1 cut(s) 347
XmnI GAANNNNTTC 1 cut(s) 17
ZrmI AGTACT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.