Rorug05G0103400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0017788
Physical Location & Seq
Forward (+)
9092708 .. 9096764
4057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0103400.1

Sequence Viewer

Length: 3585 bp
ATGGCTTCTTCTTCTTCTTCTCCTCCTCCTCCTGCGCATGACTTTAAGTATGATGTCTTTCTTAGTTTCCGAGGCGAGGACACCCGCAATACTTTTACCAGTCATCTTCATGCCGCTTTAGTGCGGAAGAAAATCAAAACCTACATAGATTACGAACTTGTGAGAGGAGAGGAAATTGCACCTGCCCTTCTCGAAGCAATAGAGGAATCAAAGCTTTCAGTGGTCATTTTGTCACAAGACTATGCTTCTTCAACCTGGTGCTTGGACGAACTTTCGCATATTTTGGTATGCAAGGAAAGCTATCAACAATTGGTTATACCCCTTTTTTACAGAGTAGATCCATCACATGTACGACACCAGGGGGAGAGTTATGCTGATGCATTTGTTCAACATGAAGAACGTTTCAAGAACACTCCACACAAGGTGCTCAAGTGGAGGGATGCTTTAACAAAAGTGTCTATACTAGCTGGGTTTGATTCACGCAACATCGGACTTGATTCCGAACTAGTTGAGTTAGTCGTCAAAGATATTTTGATGAAGTTGAATCGAGAATGCTCAAGCAATTTGACAGGCCTGGTTGGAATTGAAAGTCGAATCCAGACACTTGAGTCATTGTTATGCATTGTCCCACAAGATGTTCGTGTTCGCACGGTAGGTATTTGGGGCATGGGTGGTATAGGCAAGACCACCCTTGCTGACGCTGTATTCAACCGACTCTCATCTCAATATGAAGCTTCATGTTTTCTTGCAAATGTTAGGGAAAGAGCAGAAAAACATGGACTACACTATTTGCGAAATGAACTTCTTTGCAATTTATTGAAGGATGAAAGTCTAGGGATCAGCACTCCATCAATAGGATCAACTTTTATCAAAAAGAGGCTCTCTCGTGCAAAGGTTCTCGTTGTTCTTGATGATGTAAATAATGTAAGCCAATTAGACGTCTTAGTTGGTGATCAGTTTCAATTTGGACCAGGAAGTAGAATCATAATAACAACTAGAGATATGCACCAACTTAGAGAGATGCAACAACTGCAGAAAGGAGCAGACCATGATGTTAAAATATACAAGGCAGAGGAACTAGATCGTGATGAAGCACTTCAGCTCTTCCACTTGAATAATTCCGAAGAAATCAGCTGCAGAGCAGATTATTCCGTATTACTAAGAATGGTGGATTATGCTGCTGGAATTCCCTTGGCCCTTAAAATCTGGGGTTCCTTATTTCGTCGATGCAAGAGCACACAAGAACAGGAAAGTTTCTTGAAAAAGTTGAAAAGGTTTCCTGAAAAGAAACTTCAAAACGTGTATAGGATAAGTTATGATGCATTAGGAGAGAATGAGAGGGAGATATTTCTTTATATCGCGTGCTTTCACAAAGGAGAGGATGTTTATAGAGCGAAAGAACAATTAGATGCATGTGGATTATTTGCGGATAGTGGAATCAACGTTCTCATTGATATGTCTCTGATATCAATCAAAGACAAAGAGTGGACTGACCAAGAAGAAAAAACTCGTTACCTGTGGATGCATGATGTGATACAAGAAATGGGATGGGCAGTTGAGCATGAACAACATCCTACGAAGCCTGGAAAGCGCAGTAGGCTGTGTATCCCGGAGGACATCTGTCATGTGTTAGAAAAGAATAAGGGGACTGAAAAAGTTCAAAGCATATCCCTAAACCTCTCTCAGATTTCAAAGCTAAACTTGACTCCTCAAGCCTTCAAAAAGATGTGTAATCTAAAATTTGTAAAGTTATATGGTGAAGATCGCAGGGGCCACTCCTACTGCTACCCTAGGAAGGACTGCAATGTGCACTTTAATCTTCAGGATCTTGAATATCTTCCTGACTCCCTTATCTATCTCAGCTGTCCTGCATACACTTCGAAATATTTGCCATCAACATTTTCTGCAGAGAATCTCACTGAGCTTTCTATGCCTGGTAGCCAACTCCAGAGACTCTGGAATGTAGACCAGAAGCCTAGGAACTTAAAACGCATCGATCTCAGTCACTCCAAGCAGCTGGTTGAAGTTCCAGATCTCTCTATGAGCGTAAACATTGAGAGGATAAACCTTGAAGGCTGTGAAAGGCTGGCTGAAATTCCTTCCTATTTTGAAAATCTTCACAAGCTTACTTGGTTGAATGTGAGTAGTTGCACGAATCTTAGTGTTCTCTCAGATATGCCCTGCAATATGGTTTCGTTGAAGTTAGGTTGGACTGCAATAGAAGACTTGCCTTCATCAATTTGGTCTCAAGAAAAACTTGTTGAATTGAATCTTGATGGGTGCGAGGGGATTAAGAAACTTCCAAACAGCATTTGGAAGCTGTATTCCCTCACATCTCTTGTGTTGTGCGGGACAAGTATAGAAGGATTGCCCCCGTCAATCGAGTGTCTCTCAGGATTAGAATGTCTTGACCTGAATAGTTGCCGAAAGTTTGTCAGTCTCCCAACCAGCATTTGCAAGTTGAAATCGCTTGCGATATTTTGTCTTATTGGATGCTCTAGCTTCGAAAACTTCCCTGAGATCTTGGAGCCTATGGAGCGTCTGCACCATCTTTATTTACAAGGAACGAAATTCAAAGAGCTGCCCTCTTTGATTGAAAACATAGTTGGGCGGAAGGAACCAGGTAACACTGGGTGTAGAAGCTTTGAGTCTGTCCCAAACAGCATCTTGCCTCCGTCCTTGGTCATTTTATGGTCTTCGATGAGTATTTTAGACCTCAGTGACTGCACAATGTTAGAAGAAATCCCTGATTGCATCTTTAGCCTAAACAGCTTATTTCGATTAGATGCGAGTGAAACCATGATTAGGAGCATACCTTCAACCATCAGTCAAGCATCTCAGCTCAGGGTATTATTTGTAAAGGATTGCAAGCGCCTTGAATATTTACCCAAGCTCCCATATCTTCTACAATTGATAGATGCAAGTGGCTGCAAGAGATTGAAGACAGTGTCTGGATTCACGACTGCACTTACAGAAGGTTTGGATGAAAGAGGTCCTTGCATGTTGAATACATTTTATTATAATTGCGTTAGTTTGAATGAGAATGCAAGGAGGAACATAATGGATGATGCACATCTTAGAAATAGAGCAACAGATGACTATCCATTTTTTGTGTACGAAGTTAGTTGTCCGGGAAATGAAATTCCAAAATGGGTCAGCAATCAAATGGAGGGATCTTCGATAGATATAAAGCTTCCTCTACATTGGTCTGAAGATGCAAACTTTTTGGGTTTAGCTTTCTATGTTGTTGTTGACTTCCATAAAGCAAGCAATCCGGTCTGTGCGGCTGAATGCATTCTCGAAACCGAAAATGGTGAAGACCATGCAATTCTGATTAGCCAGAATCAGAGTCATTGTCTACATTATGATGACGACAACTCAGATCACCTGGTGGTGTGGTACGATTCCCTGCCGATGGTGAGGTCCACTCCTTATGCCACCCATGCCTCTTTTCATTTCTATGTACATGAAGAGCAGCAGTGCTCGGTTGAGTATCTGGTGAAAAGGTGTGGGGTGGCCTTTGTGTATGCTCAAGGCCGAGGCCGAAAAGCTACCACTTTTGAATCCATGTTTCGAAAACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1194

Amino Acids

135.9

Weight (kDa)

5.88

Isoelectric Point (pI)

51.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 195 4.1e-53 TIR domain
TIR_2 PF13676 19 - 129 1.3e-14 TIR domain
NB-ARC PF00931 216 - 370 3.7e-14 NB-ARC domain
WHD_ROQ1 PF23282 444 - 519 7.4e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 756 - 853 1e-10 Leucine-rich repeat region
LRR_13 PF23286 756 - 834 8.3e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 1043 - 1178 5.1e-13 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3021
AarI CACCTGC 1 cut(s) 190
AasI GACNNNNNNGTC 1 cut(s) 607
AatII GACGTC 1 cut(s) 942
Acc16I TGCGCA 1 cut(s) 36
Acc36I ACCTGC 1 cut(s) 190
AccI GTMKAC 2 cut(s) 1965, 3358
AccII CGCG 1 cut(s) 1361
AciI CCGC 7 cut(s) 85, 114, 124, 1427, 2349, 2611, 3284
AclI AACGTT 2 cut(s) 400, 1443
AclWI GGATC 5 cut(s) 332, 845, 865, 1833, 3181
AcsI RAATTY 5 cut(s) 1185, 1739, 2094, 2570, 3141
AcuI CTGAAG 3 cut(s) 1082, 1805, 3231
AcyI GRCGYC 1 cut(s) 939
AdeI CACNNNGTG 3 cut(s) 424, 2634, 3391
AfaI GTAC 4 cut(s) 351, 3116, 3401, 3465
AfiI CCNNNNNNNGG 5 cut(s) 76, 854, 1795, 2805, 3415
AflIII ACRYGT 2 cut(s) 346, 1299
AhlI ACTAGT 1 cut(s) 505
AjnI CCWGG 8 cut(s) 254, 357, 573, 970, 1582, 1933, 2620, 3387
AjuI GAANNNNNNNTTGG 2 cut(s) 2589, 2621
AloI GAACNNNNNNTCC 2 cut(s) 1428, 1460
Alw21I GWGCWC 4 cut(s) 429, 1238, 1812, 3485
Alw26I GTCTC 5 cut(s) 1464, 1945, 2250, 2393, 2444
Alw44I GTGCAC 1 cut(s) 1808
AlwI GGATC 5 cut(s) 332, 845, 865, 1833, 3181
AlwNI CAGNNNCTG 2 cut(s) 2724, 2951
AoxI GGCC 6 cut(s) 571, 1194, 1771, 3516, 3535, 3541
ApaLI GTGCAC 1 cut(s) 1808
ApeKI GCWGC 6 cut(s) 1134, 1178, 2014, 2581, 2928, 3475
ApoI RAATTY 5 cut(s) 1185, 1739, 2094, 2570, 3141
Asp700I GAANNNNTTC 5 cut(s) 1095, 1656, 1836, 2115, 3294
AspA2I CCTAGG 2 cut(s) 1790, 1976
AspLEI GCGC 3 cut(s) 37, 1593, 2874
AspS9I GGNCC 5 cut(s) 968, 1195, 1771, 2993, 3423
AsuC2I CCSGG 2 cut(s) 1610, 3132
AsuHPI GGTGA 6 cut(s) 962, 1769, 3326, 3377, 3430, 3511
AsuII TTCGAA 3 cut(s) 1880, 2505, 3574
AvaII GGWCC 3 cut(s) 968, 2993, 3423
AvrII CCTAGG 2 cut(s) 1790, 1976
BaeGI GKGCMC 1 cut(s) 1812
BauI CACGAG 1 cut(s) 885
BbsI GAAGAC 4 cut(s) 2229, 2688, 2948, 3324
Bbv12I GWGCWC 4 cut(s) 429, 1238, 1812, 3485
BbvI GCAGC 6 cut(s) 1121, 1165, 2026, 2568, 2915, 3487
BccI CCATC 8 cut(s) 349, 856, 1542, 1900, 2270, 2556, 2831, 3409
BcgI CGANNNNNNTGC 4 cut(s) 1860, 1870, 1894, 1904
BciT130I CCWGG 8 cut(s) 256, 359, 575, 972, 1584, 1935, 2622, 3389
BciVI GTATCC 1 cut(s) 1616
BclI TGATCA 1 cut(s) 952
BcnI CCSGG 2 cut(s) 1610, 3132
BcoDI GTCTC 5 cut(s) 1464, 1945, 2250, 2393, 2444
BcuI ACTAGT 1 cut(s) 505
BfaI CTAG 8 cut(s) 464, 506, 833, 996, 1079, 1791, 1977, 2499
BfmI CTRYAG 3 cut(s) 1031, 1135, 1905
BfoI RGCGCY 1 cut(s) 2875
BfuAI ACCTGC 1 cut(s) 190
BfuI GTATCC 1 cut(s) 1616
BglII AGATCT 2 cut(s) 2032, 2520
BisI GCNGC 8 cut(s) 114, 1135, 1179, 2015, 2582, 2929, 3285, 3476
BlnI CCTAGG 2 cut(s) 1790, 1976
BlsI GCNGC 8 cut(s) 115, 1136, 1180, 2016, 2583, 2930, 3286, 3477
Bme18I GGWCC 3 cut(s) 968, 2993, 3423
BmgT120I GGNCC 5 cut(s) 968, 1195, 1771, 2993, 3423
BmiI GGNNCC 4 cut(s) 1213, 1772, 2529, 2619
BmrI ACTGGG 1 cut(s) 2640
BmuI ACTGGG 1 cut(s) 2640
BoxI GACNNNNGTC 1 cut(s) 1620
BpiI GAAGAC 4 cut(s) 2229, 2688, 2948, 3324
BplI GAGNNNNNCTC 8 cut(s) 868, 900, 2375, 2407, 2570, 2602, 3412, 3444
BpmI CTGGAG 1 cut(s) 1931
Bpu10I CCTNAGC 1 cut(s) 2843
Bpu14I TTCGAA 3 cut(s) 1880, 2505, 3574
BpuEI CTTGAG 6 cut(s) 413, 541, 626, 1695, 2232, 3516
BpuMI CCSGG 2 cut(s) 1610, 3132
Bsa29I ATCGAT 1 cut(s) 1995
BsaBI GATNNNNATC 3 cut(s) 1469, 3072, 3099
BsaHI GRCGYC 1 cut(s) 939
BsaI GGTCTC 1 cut(s) 2250
BsaJI CCNNGG 7 cut(s) 70, 358, 1191, 1790, 1976, 2679, 3538
BsaWI WCCGGW 1 cut(s) 3274
BsaXI ACNNNNNCTCC 4 cut(s) 1368, 1398, 2877, 2907
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 854, 1795, 2805, 3415
Bse1I ACTGG 2 cut(s) 99, 2635
Bse3DI GCAATG 1 cut(s) 1810
Bse8I GATNNNNATC 3 cut(s) 1469, 3072, 3099
BseBI CCWGG 8 cut(s) 256, 359, 575, 972, 1584, 1935, 2622, 3389
BseCI ATCGAT 1 cut(s) 1995
BseDI CCNNGG 7 cut(s) 70, 358, 1191, 1790, 1976, 2679, 3538
BseGI GGATG 9 cut(s) 445, 829, 1387, 1527, 1553, 1570, 2498, 2989, 3070
BseJI GATNNNNATC 3 cut(s) 1469, 3072, 3099
BseLI CCNNNNNNNGG 5 cut(s) 76, 854, 1795, 2805, 3415
BseMI GCAATG 1 cut(s) 1810
BseNI ACTGG 2 cut(s) 99, 2635
BseRI GAGGAG 5 cut(s) 12, 15, 18, 180, 1698
BseSI GKGCMC 1 cut(s) 1812
BseXI GCAGC 6 cut(s) 1121, 1165, 2026, 2568, 2915, 3487
BseYI CCCAGC 1 cut(s) 467
BsgI GTGCAG 3 cut(s) 2528, 2710, 2949
Bsh1236I CGCG 1 cut(s) 1361
BshFI GGCC 6 cut(s) 573, 1196, 1773, 3518, 3537, 3543
BshVI ATCGAT 1 cut(s) 1995
BsiHKAI GWGCWC 4 cut(s) 429, 1238, 1812, 3485
BsiSI CCGG 3 cut(s) 1610, 3131, 3275
BslFI GGGAC 4 cut(s) 611, 1660, 2365, 2639
BslI CCNNNNNNNGG 5 cut(s) 76, 854, 1795, 2805, 3415
BsmAI GTCTC 5 cut(s) 1464, 1945, 2250, 2393, 2444
BsmFI GGGAC 4 cut(s) 611, 1660, 2365, 2639
BsmI GAATGC 4 cut(s) 557, 3049, 3294, 3296
BsnI GGCC 6 cut(s) 573, 1196, 1773, 3518, 3537, 3543
Bso31I GGTCTC 1 cut(s) 2250
Bsp119I TTCGAA 3 cut(s) 1880, 2505, 3574
Bsp1286I GDGCHC 4 cut(s) 429, 1238, 1812, 3485
Bsp1407I TGTACA 1 cut(s) 3463
BspACI CCGC 7 cut(s) 85, 114, 124, 1427, 2349, 2611, 3284
BspANI GGCC 6 cut(s) 573, 1196, 1773, 3518, 3537, 3543
BspDI ATCGAT 1 cut(s) 1995
BspFNI CGCG 1 cut(s) 1361
BspLI GGNNCC 4 cut(s) 1213, 1772, 2529, 2619
BspMAI CTGCAG 3 cut(s) 1035, 1139, 1909
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 5 cut(s) 332, 845, 865, 1833, 3181
BspQI GCTCTTC 2 cut(s) 1109, 3465
BspT104I TTCGAA 3 cut(s) 1880, 2505, 3574
BspTNI GGTCTC 1 cut(s) 2250
BsrDI GCAATG 1 cut(s) 1810
BsrGI TGTACA 1 cut(s) 3463
BsrI ACTGG 2 cut(s) 99, 2635
BssECI CCNNGG 7 cut(s) 70, 358, 1191, 1790, 1976, 2679, 3538
BssNI GRCGYC 1 cut(s) 939
BssSI CACGAG 1 cut(s) 885
BssT1I CCWWGG 4 cut(s) 1191, 1790, 1976, 2679
Bst2BI CACGAG 1 cut(s) 885
Bst2UI CCWGG 8 cut(s) 256, 359, 575, 972, 1584, 1935, 2622, 3389
Bst4CI ACNGT 2 cut(s) 652, 2947
Bst6I CTCTTC 2 cut(s) 1109, 3465
BstACI GRCGYC 1 cut(s) 939
BstAPI GCANNNNNTGC 1 cut(s) 1030
BstAUI TGTACA 1 cut(s) 3463
BstBI TTCGAA 3 cut(s) 1880, 2505, 3574
BstC8I GCNNGC 5 cut(s) 1363, 2088, 2472, 2870, 3268
BstF5I GGATG 9 cut(s) 445, 829, 1387, 1527, 1553, 1570, 2498, 2989, 3070
BstFNI CGCG 1 cut(s) 1361
BstH2I RGCGCY 1 cut(s) 2875
BstHHI GCGC 3 cut(s) 37, 1593, 2874
BstMAI GTCTC 5 cut(s) 1464, 1945, 2250, 2393, 2444
BstMWI GCNNNNNNNGC 9 cut(s) 297, 1030, 1588, 1597, 1930, 2536, 2760, 2769, 3443
BstNI CCWGG 8 cut(s) 256, 359, 575, 972, 1584, 1935, 2622, 3389
BstNSI RCATGY 3 cut(s) 350, 1416, 3004
BstPAI GACNNNNGTC 1 cut(s) 1620
BstSFI CTRYAG 3 cut(s) 1031, 1135, 1905
BstSLI GKGCMC 1 cut(s) 1812
BstUI CGCG 1 cut(s) 1361
BstV1I GCAGC 6 cut(s) 1121, 1165, 2026, 2568, 2915, 3487
BstV2I GAAGAC 4 cut(s) 2229, 2688, 2948, 3324
BstX2I RGATCY 5 cut(s) 337, 1825, 2032, 2520, 3173
BstXI CCANNNNNNTGG 1 cut(s) 2017
BstYI RGATCY 5 cut(s) 337, 1825, 2032, 2520, 3173
Bsu15I ATCGAT 1 cut(s) 1995
BsuI GTATCC 1 cut(s) 1616
BsuRI GGCC 6 cut(s) 573, 1196, 1773, 3518, 3537, 3543
BsuTUI ATCGAT 1 cut(s) 1995
BtsCI GGATG 9 cut(s) 445, 829, 1387, 1527, 1553, 1570, 2498, 2989, 3070
BtsI GCAGTG 1 cut(s) 3485
BtsIMutI CAGTG 6 cut(s) 225, 1917, 2628, 2725, 2952, 3485
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 5 cut(s) 1363, 2088, 2472, 2870, 3268
CaiI CAGNNNCTG 2 cut(s) 2724, 2951
CfoI GCGC 3 cut(s) 37, 1593, 2874
Cfr13I GGNCC 5 cut(s) 968, 1195, 1771, 2993, 3423
ClaI ATCGAT 1 cut(s) 1995
CseI GACGC 2 cut(s) 707, 2528
CsiI ACCWGGT 3 cut(s) 254, 2620, 3387
Csp6I GTAC 4 cut(s) 350, 3115, 3400, 3464
CviQI GTAC 4 cut(s) 350, 3115, 3400, 3464
DraIII CACNNNGTG 3 cut(s) 424, 2634, 3391
DrdI GACNNNNNNGTC 1 cut(s) 607
DseDI GACNNNNNNGTC 1 cut(s) 607
Eam1104I CTCTTC 2 cut(s) 1109, 3465
EarI CTCTTC 2 cut(s) 1109, 3465
EciI GGCGGA 1 cut(s) 2626
Eco130I CCWWGG 4 cut(s) 1191, 1790, 1976, 2679
Eco147I AGGCCT 1 cut(s) 573
Eco31I GGTCTC 1 cut(s) 2250
Eco32I GATATC 1 cut(s) 1467
Eco47I GGWCC 3 cut(s) 968, 2993, 3423
Eco57I CTGAAG 3 cut(s) 1082, 1805, 3231
EcoO109I RGGNCCY 1 cut(s) 2993
EcoRI GAATTC 1 cut(s) 1185
EcoRII CCWGG 8 cut(s) 254, 357, 573, 970, 1582, 1933, 2620, 3387
EcoRV GATATC 1 cut(s) 1467
EcoT14I CCWWGG 4 cut(s) 1191, 1790, 1976, 2679
EcoT22I ATGCAT 6 cut(s) 382, 623, 1324, 1414, 1527, 3296
ErhI CCWWGG 4 cut(s) 1191, 1790, 1976, 2679
FalI AAGNNNNNCTT 6 cut(s) 1685, 1717, 2241, 2273, 2980, 3012
FaqI GGGAC 4 cut(s) 611, 1660, 2365, 2639
FauI CCCGC 2 cut(s) 92, 2342
FbaI TGATCA 1 cut(s) 952
FblI GTMKAC 2 cut(s) 1965, 3358
Fnu4HI GCNGC 8 cut(s) 114, 1135, 1179, 2015, 2582, 2929, 3285, 3476
FokI GGATG 9 cut(s) 452, 836, 1394, 1534, 1557, 1560, 2505, 2996, 3077
Fsp4HI GCNGC 8 cut(s) 114, 1135, 1179, 2015, 2582, 2929, 3285, 3476
FspBI CTAG 8 cut(s) 464, 506, 833, 996, 1079, 1791, 1977, 2499
FspI TGCGCA 1 cut(s) 36
GlaI GCGC 3 cut(s) 36, 1592, 2873
GluI GCNGC 8 cut(s) 114, 1135, 1179, 2015, 2582, 2929, 3285, 3476
GsaI CCCAGC 1 cut(s) 471
GsuI CTGGAG 1 cut(s) 1931
HaeII RGCGCY 1 cut(s) 2875
HaeIII GGCC 6 cut(s) 573, 1196, 1773, 3518, 3537, 3543
HapII CCGG 3 cut(s) 1610, 3131, 3275
HgaI GACGC 2 cut(s) 707, 2528
HhaI GCGC 3 cut(s) 37, 1593, 2874
Hin1I GRCGYC 1 cut(s) 939
Hin6I GCGC 3 cut(s) 35, 1591, 2872
HinP1I GCGC 3 cut(s) 35, 1591, 2872
HincII GTYRAC 1 cut(s) 3253
HindII GTYRAC 1 cut(s) 3253
HindIII AAGCTT 5 cut(s) 212, 732, 2123, 2641, 3191
HpaII CCGG 3 cut(s) 1610, 3131, 3275
HphI GGTGA 6 cut(s) 962, 1769, 3326, 3377, 3430, 3511
Hpy166II GTNNAC 8 cut(s) 1488, 1810, 1966, 2050, 3115, 3253, 3359, 3426
Hpy8I GTNNAC 8 cut(s) 1488, 1810, 1966, 2050, 3115, 3253, 3359, 3426
Hpy99I CGWCG 1 cut(s) 1227
HpyCH4III ACNGT 2 cut(s) 652, 2947
HpyCH4IV ACGT 4 cut(s) 400, 939, 1299, 1443
HpyF10VI GCNNNNNNNGC 9 cut(s) 297, 1030, 1588, 1597, 1930, 2536, 2760, 2769, 3443
HpySE526I ACGT 4 cut(s) 400, 939, 1299, 1443
Hsp92I GRCGYC 1 cut(s) 939
HspAI GCGC 3 cut(s) 35, 1591, 2872
Ksp22I TGATCA 1 cut(s) 952
LguI GCTCTTC 2 cut(s) 1109, 3465
LmnI GCTCC 5 cut(s) 1040, 2527, 2536, 2808, 2898
Lsp1109I GCAGC 6 cut(s) 1121, 1165, 2026, 2568, 2915, 3487
MabI ACCWGGT 3 cut(s) 254, 2620, 3387
MaeI CTAG 8 cut(s) 464, 506, 833, 996, 1079, 1791, 1977, 2499
MaeII ACGT 4 cut(s) 400, 939, 1299, 1443
MaeIII GTNAC 5 cut(s) 231, 1511, 2003, 2624, 2720
MfeI CAATTG 2 cut(s) 308, 2909
MflI RGATCY 5 cut(s) 337, 1825, 2032, 2520, 3173
MhlI GDGCHC 4 cut(s) 429, 1238, 1812, 3485
MlyI GAGTC 7 cut(s) 617, 708, 1699, 1838, 1947, 2657, 3358
MmeI TCCRAC 2 cut(s) 559, 2189
Mph1103I ATGCAT 6 cut(s) 382, 623, 1324, 1414, 1527, 3296
MroXI GAANNNNTTC 5 cut(s) 1095, 1656, 1836, 2115, 3294
MseI TTAA 7 cut(s) 45, 446, 1056, 1200, 1815, 1985, 2292
MslI CAYNNNNRTG 5 cut(s) 108, 616, 1454, 3366, 3459
MspA1I CMGCKG 3 cut(s) 1134, 1863, 2017
MspI CCGG 3 cut(s) 1610, 3131, 3275
MunI CAATTG 2 cut(s) 308, 2909
Mva1269I GAATGC 4 cut(s) 557, 3049, 3294, 3296
MvaI CCWGG 8 cut(s) 256, 359, 575, 972, 1584, 1935, 2622, 3389
MvnI CGCG 1 cut(s) 1361
MwoI GCNNNNNNNGC 9 cut(s) 297, 1030, 1588, 1597, 1930, 2536, 2760, 2769, 3443
NciI CCSGG 2 cut(s) 1610, 3132
NlaIV GGNNCC 4 cut(s) 1213, 1772, 2529, 2619
NmeAIII GCCGAG 1 cut(s) 3563
NmuCI GTSAC 3 cut(s) 231, 2003, 2720
NsbI TGCGCA 1 cut(s) 36
NsiI ATGCAT 6 cut(s) 382, 623, 1324, 1414, 1527, 3296
NspI RCATGY 3 cut(s) 350, 1416, 3004
NspV TTCGAA 3 cut(s) 1880, 2505, 3574
PaqCI CACCTGC 1 cut(s) 190
PceI AGGCCT 1 cut(s) 573
PciI ACATGT 1 cut(s) 346
PciSI GCTCTTC 2 cut(s) 1109, 3465
PctI GAATGC 4 cut(s) 557, 3049, 3294, 3296
PdmI GAANNNNTTC 5 cut(s) 1095, 1656, 1836, 2115, 3294
PflFI GACNNNGTC 1 cut(s) 2947
PfoI TCCNGGA 2 cut(s) 1608, 3130
PkrI GCNGC 8 cut(s) 115, 1136, 1180, 2016, 2583, 2930, 3286, 3477
PleI GAGTC 7 cut(s) 616, 708, 1699, 1838, 1947, 2656, 3357
PpsI GAGTC 7 cut(s) 616, 708, 1699, 1838, 1947, 2656, 3357
PpuMI RGGWCCY 1 cut(s) 2993
PscI ACATGT 1 cut(s) 346
PshAI GACNNNNGTC 1 cut(s) 1620
PsiI TTATAA 1 cut(s) 3021
Psp1406I AACGTT 2 cut(s) 400, 1443
Psp5II RGGWCCY 1 cut(s) 2993
Psp6I CCWGG 8 cut(s) 254, 357, 573, 970, 1582, 1933, 2620, 3387
PspFI CCCAGC 1 cut(s) 467
PspGI CCWGG 8 cut(s) 254, 357, 573, 970, 1582, 1933, 2620, 3387
PspN4I GGNNCC 4 cut(s) 1213, 1772, 2529, 2619
PspPI GGNCC 5 cut(s) 968, 1195, 1771, 2993, 3423
PspPPI RGGWCCY 1 cut(s) 2993
PstI CTGCAG 3 cut(s) 1035, 1139, 1909
PstNI CAGNNNCTG 2 cut(s) 2724, 2951
PsuI RGATCY 5 cut(s) 337, 1825, 2032, 2520, 3173
PsyI GACNNNGTC 1 cut(s) 2947
PvuII CAGCTG 3 cut(s) 1134, 1863, 2017
RsaI GTAC 4 cut(s) 351, 3116, 3401, 3465
RsaNI GTAC 4 cut(s) 350, 3115, 3400, 3464
RseI CAYNNNNRTG 5 cut(s) 108, 616, 1454, 3366, 3459
SapI GCTCTTC 2 cut(s) 1109, 3465
SaqAI TTAA 7 cut(s) 45, 446, 1056, 1200, 1815, 1985, 2292
SatI GCNGC 8 cut(s) 114, 1135, 1179, 2015, 2582, 2929, 3285, 3476
Sau96I GGNCC 5 cut(s) 968, 1195, 1771, 2993, 3423
SchI GAGTC 7 cut(s) 617, 708, 1699, 1838, 1947, 2657, 3358
SduI GDGCHC 4 cut(s) 429, 1238, 1812, 3485
SexAI ACCWGGT 3 cut(s) 254, 2620, 3387
SfcI CTRYAG 3 cut(s) 1031, 1135, 1905
SfuI TTCGAA 3 cut(s) 1880, 2505, 3574
SinI GGWCC 3 cut(s) 968, 2993, 3423
SmiMI CAYNNNNRTG 5 cut(s) 108, 616, 1454, 3366, 3459
SmlI CTYRAG 6 cut(s) 428, 556, 605, 1710, 2247, 3531
SmoI CTYRAG 6 cut(s) 428, 556, 605, 1710, 2247, 3531
SpeI ACTAGT 1 cut(s) 505
SseBI AGGCCT 1 cut(s) 573
SsiI CCGC 7 cut(s) 85, 114, 124, 1427, 2349, 2611, 3284
SspI AATATT 2 cut(s) 1886, 2882
SspMI CTAG 8 cut(s) 464, 506, 833, 996, 1079, 1791, 1977, 2499
StuI AGGCCT 1 cut(s) 573
StyI CCWWGG 4 cut(s) 1191, 1790, 1976, 2679
TaaI ACNGT 2 cut(s) 652, 2947
TaiI ACGT 4 cut(s) 403, 942, 1302, 1446
TatI WGTACW 1 cut(s) 3463
TauI GCSGC 2 cut(s) 116, 3287
Tru1I TTAA 7 cut(s) 45, 446, 1056, 1200, 1815, 1985, 2292
Tru9I TTAA 7 cut(s) 45, 446, 1056, 1200, 1815, 1985, 2292
TscAI CASTG 6 cut(s) 225, 1924, 2635, 2725, 2952, 3485
TseFI GTSAC 3 cut(s) 231, 2003, 2720
TseI GCWGC 6 cut(s) 1134, 1178, 2014, 2581, 2928, 3475
Tsp45I GTSAC 3 cut(s) 231, 2003, 2720
TspGWI ACGGA 2 cut(s) 1141, 2664
TspRI CASTG 6 cut(s) 225, 1924, 2635, 2725, 2952, 3485
Tth111I GACNNNGTC 1 cut(s) 2947
VneI GTGCAC 1 cut(s) 1808
VpaK11BI GGWCC 3 cut(s) 968, 2993, 3423
XapI RAATTY 5 cut(s) 1185, 1739, 2094, 2570, 3141
XceI RCATGY 3 cut(s) 350, 1416, 3004
XcmI CCANNNNNNNNNTGG 1 cut(s) 2310
XmaJI CCTAGG 2 cut(s) 1790, 1976
XmiI GTMKAC 2 cut(s) 1965, 3358
XmnI GAANNNNTTC 5 cut(s) 1095, 1656, 1836, 2115, 3294
XspI CTAG 8 cut(s) 464, 506, 833, 996, 1079, 1791, 1977, 2499
ZraI GACGTC 1 cut(s) 940
Zsp2I ATGCAT 6 cut(s) 382, 623, 1324, 1414, 1527, 3296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.