Rorug05G0125200
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0023005
Physical Location & Seq
Reverse (-)
11134027 .. 11138911
4885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0125200.1

Sequence Viewer

Length: 2331 bp
ATGGCCTCATTACATGAGAGGATTCTTGTTACGCTGCTCTATTTGTTAATGTTAGTGGCCGCAACAACAACATCAACAACACTGCTAGCAGCAGCGCAGACCCTACCGCCGCCTCCTCAAGCCAAGTCTGGGTGCAAAGAGAAGTGTGGTGATCTCACAATTCCATATCCATTTGGCATAGGTGAAAATTGTTCCATGCGACCGGAATTCACCATCGCTTGTGACCAATCCACCACTGTATCCCCATCGGCAAACTTCACAAATGGTACCTTCACCTTTCCCATCACCATTACTAAATTTTCCCTTGATGACGGCGAGTTGCAAGTAATGCAGGACATAGCACGAAAATGTTATGATGACACTAAAACAAACAGCAGCAGTTCACTGGAGCTGCCTGCTCCTTATATCATCTCAACCAAAAACAACTTCTTTACCCTTGGCTGCAACGTTTTATCGCTGTATCAACAAGTTGTAGATGAAGACTCCCAAACAGGTGTAAGTGTTGCCTTCTGTGAGGATAATATTCTTGGCAAAGAAATACCCGAGTCATGCACCGGCTTCGGGTGTGCCCAAACTTCCATCCCAAGTGGACTGCAAAACATTTCCATTGGAATGGCTGCACTTTCCGACTACAACGCGAGTGATCACTGGGCATCAACTTACAAGTGCAGCTACGCCTTCATCGTTGAAGAAGGCAACTTCACATTCGCACCGAAAACAAGTTTTCAACAACTGAATACAACAATTCAGCAGCTTCCGGTGGCTGTTAATTGGGCAATCGGGGACGAGCCATGTGCAGTAGCGAAGAACCTAACTGATTATTCGTGCAAAGAGAATACTACTTGTGTCGACCGGAGCACCGTCACTAATGAGACGGCTGCAGGTTACATTTGCCGGTGCAAGCCAGGCTACGAAGGGAACCCATACCTCGGTTGCCAAGATATCGATGAGTGCGCGACCTCAAGCGGCCACTGTCAGAACGGAAAGTGCATAAATTCACCTCCAGGAAATTACTCGTGCAAATGTAATAAAGGATTCAGACACCGAGACGAATTCACTTGTGAGACCTGGTCAAACAACGCTTCCCTAAAAATCTCATTAGGTGTCAGTATAGGCTTCTTGGTTTTGGTGGTCTTAATTTCGTGGATATATTGGGAAATGAACAAAAGAAGGCTCATCAAACTCAAAAAGAAGTACTTCAAAGAAAACGGTGGCTTAACATTACAACAACAACTCGCTAGTCATGGAGGAGCCGTGGAAACAACAAAAATCTTTACAGCGGAAGAACTTGAGAAGGCGACAAACAATTACCATGCAGATGAGATTCTTGGTGAAGGAGCCTATGGAACAGTTTACAAAGGAATTCTACCTGATAAGAAAGTTGTTGCCATAAAAAAATCAAAAATTGGCGCCTCAACTCAAAGTGAGCAGTTCGTTAATGAGGTTATCGTTCTCTCTCAAATCAACCACAGAAATGTAGTAAAACTATTGGGTTGTTGTTTCGAGACTGAAGTGCCTTTACTAGTTTATGAGTACATTACCCATGGCACTCTTTTTGAGCATATATTCAATAAGGGAAAAGGATCACCGCTTTCATGGGAATTACGATTGAAGATAGCATCAGAAACGGCAGGGGCACTAGCTTACTTACACTCCTCTACTTCCACACCAATCATACATCGAGACGTGAAAGCAACAAATATACTATTAGATGACAATTACACGGCCAAAGTGTCCGATTTTGGAGCTTCGCGTTTCATTCCTCTAGATCAAACTGAACTAGCAACACTAGTGCAAGGGACGCTTGGCTACTTGGACCCTGAATACTTTCATTCCAATCAACTGACTGAAAAGAGTGACGTCTACAGCTTTGGAGTTGTCCTAGCAGAGTTATTAACGAGCAGAGTGGCACTTTCTTTCGCTAGACCTGACGCAGAGAGATGCCTAGCAAGTTTCTTTGTTTCTTCAATAGAGAAAGATTGCTTGATTGAAATTCTTGACGCTGACATAGTGAATGAGGGGAACATAGACACAGCAGAGCAAGTTGCTTATCTCGCAAAAAGATGTCTAGGGATAAGAGGGGAGGAAAGGCCTTCAATGAAAGAAGTGGCCATGGAGTTGGAGGGAATGCAAAATACAGCGAAGCATCCATGGGGAAATAATGCAAATTCATGTCCCGAGGAGACTGAGTGCTTGCTGGGGTCACCAATCAATTCAGAAGCTTACATTGTGAATGTTAAAGGTGATGGTGATGAAGGTTCTAGTGGTACAACTAGTGGGTATGACAGCATGCAAATTCAAATGTTAATGCCATATGATGATGTGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

776

Amino Acids

85.0

Weight (kDa)

4.98

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 45 - 109 4.2e-09 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 314 - 354 5.4e-06 Calcium-binding EGF domain
Pkinase PF00069 437 - 703 1.1e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 439 - 707 1e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0023005)

Species Orthologous Gene IDs
rosa_laevigata RLG00000033305
rosa_multiflora Rmu_sc0014478.1_g000007
rosa_rugosa Rorug05G0125200
rosa_samantha Rh5AG218000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1863
Acc36I ACCTGC 1 cut(s) 872
Acc65I GGTACC 1 cut(s) 266
AccB1I GGYRCC 2 cut(s) 266, 1409
AccI GTMKAC 2 cut(s) 849, 1863
AccII CGCG 3 cut(s) 638, 956, 1753
AciI CCGC 6 cut(s) 60, 107, 110, 966, 1280, 1589
AclI AACGTT 1 cut(s) 447
AclWI GGATC 1 cut(s) 1591
AcoI YGGCCR 4 cut(s) 57, 967, 1725, 2109
AcsI RAATTY 8 cut(s) 206, 296, 994, 1052, 1362, 1992, 2167, 2295
AcuI CTGAAG 1 cut(s) 1530
AcyI GRCGYC 2 cut(s) 1410, 1860
AfaI GTAC 4 cut(s) 268, 1196, 1535, 2269
AfiI CCNNNNNNNGG 3 cut(s) 129, 561, 929
AgsI TTSAA 9 cut(s) 689, 728, 1201, 1570, 1612, 1968, 1991, 2097, 2300
AhlI ACTAGT 3 cut(s) 1522, 1789, 2273
AjiI CACGTC 1 cut(s) 1687
AjnI CCWGG 3 cut(s) 904, 1003, 1067
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AluBI AGCT 7 cut(s) 391, 672, 754, 1643, 1748, 1869, 2222
AluI AGCT 7 cut(s) 391, 672, 754, 1643, 1748, 1869, 2222
Alw21I GWGCWC 1 cut(s) 860
Alw26I GTCTC 6 cut(s) 866, 1041, 1058, 1499, 1677, 2177
AlwI GGATC 1 cut(s) 1591
Ama87I CYCGRG 2 cut(s) 542, 2177
AoxI GGCC 6 cut(s) 3, 57, 967, 1725, 2090, 2109
ApoI RAATTY 8 cut(s) 206, 296, 994, 1052, 1362, 1992, 2167, 2295
Asp700I GAANNNNTTC 2 cut(s) 1196, 1827
Asp718I GGTACC 1 cut(s) 266
AspLEI GCGC 3 cut(s) 97, 956, 1412
AspS9I GGNCC 1 cut(s) 1816
AsuNHI GCTAGC 1 cut(s) 85
AvaI CYCGRG 2 cut(s) 542, 2177
AvaII GGWCC 1 cut(s) 1816
BaeGI GKGCMC 2 cut(s) 571, 1639
BaeI ACNNNNGTAYC 2 cut(s) 222, 255
BalI TGGCCA 1 cut(s) 2111
BanI GGYRCC 2 cut(s) 266, 1409
BarI GAAGNNNNNNTAC 2 cut(s) 1503, 1535
BauI CACGAG 1 cut(s) 1015
BbsI GAAGAC 1 cut(s) 486
Bbv12I GWGCWC 1 cut(s) 860
BccI CCATC 5 cut(s) 221, 253, 290, 587, 2240
BceAI ACGGC 5 cut(s) 328, 891, 1238, 1644, 1740
BcgI CGANNNNNNTGC 4 cut(s) 541, 575, 776, 810
BciT130I CCWGG 3 cut(s) 906, 1005, 1069
BciVI GTATCC 1 cut(s) 250
BclI TGATCA 1 cut(s) 643
BcoDI GTCTC 6 cut(s) 866, 1041, 1058, 1499, 1677, 2177
BcuI ACTAGT 3 cut(s) 1522, 1789, 2273
BfmI CTRYAG 2 cut(s) 879, 1864
BfoI RGCGCY 1 cut(s) 1413
BfuAI ACCTGC 1 cut(s) 872
BfuI GTATCC 1 cut(s) 250
BmcAI AGTACT 1 cut(s) 1196
Bme1390I CCNGG 3 cut(s) 906, 1005, 1069
Bme18I GGWCC 1 cut(s) 1816
BmeT110I CYCGRG 2 cut(s) 542, 2177
BmgBI CACGTC 1 cut(s) 1687
BmgT120I GGNCC 1 cut(s) 1816
BmiI GGNNCC 6 cut(s) 268, 920, 1252, 1339, 1411, 1818
BmrFI CCNGG 3 cut(s) 906, 1005, 1069
BmrI ACTGGG 1 cut(s) 658
BmsI GCATC 4 cut(s) 662, 1628, 1931, 2155
BmtI GCTAGC 1 cut(s) 89
BmuI ACTGGG 1 cut(s) 658
BpiI GAAGAC 1 cut(s) 486
BpmI CTGGAG 2 cut(s) 407, 987
BpuEI CTTGAG 3 cut(s) 102, 946, 1310
Bsa29I ATCGAT 1 cut(s) 945
BsaHI GRCGYC 2 cut(s) 1410, 1860
BsaI GGTCTC 1 cut(s) 1058
BsaJI CCNNGG 7 cut(s) 436, 928, 1254, 1543, 2112, 2150, 2178
BsaWI WCCGGW 3 cut(s) 202, 757, 852
BsaXI ACNNNNNCTCC 6 cut(s) 1239, 1269, 1637, 1667, 2174, 2204
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 561, 929
Bse118I RCCGGY 2 cut(s) 554, 894
Bse1I ACTGG 2 cut(s) 390, 653
BseBI CCWGG 3 cut(s) 906, 1005, 1069
BseCI ATCGAT 1 cut(s) 945
BseDI CCNNGG 7 cut(s) 436, 928, 1254, 1543, 2112, 2150, 2178
BseGI GGATG 2 cut(s) 579, 2146
BseLI CCNNNNNNNGG 3 cut(s) 129, 561, 929
BseMII CTCAG 1 cut(s) 2178
BseNI ACTGG 2 cut(s) 390, 653
BseRI GAGGAG 4 cut(s) 105, 1263, 1645, 2195
BseSI GKGCMC 2 cut(s) 571, 1639
BseYI CCCAGC 1 cut(s) 2197
BsgI GTGCAG 3 cut(s) 603, 688, 816
Bsh1236I CGCG 3 cut(s) 638, 956, 1753
Bsh1285I CGRYCG 2 cut(s) 203, 853
BshFI GGCC 6 cut(s) 5, 59, 969, 1727, 2092, 2111
BshNI GGYRCC 2 cut(s) 266, 1409
BshVI ATCGAT 1 cut(s) 945
BsiEI CGRYCG 2 cut(s) 203, 853
BsiHKAI GWGCWC 1 cut(s) 860
BsiHKCI CYCGRG 2 cut(s) 542, 2177
BsiSI CCGG 5 cut(s) 203, 555, 758, 853, 895
BslFI GGGAC 3 cut(s) 797, 1813, 2160
BslI CCNNNNNNNGG 3 cut(s) 129, 561, 929
BsmAI GTCTC 6 cut(s) 866, 1041, 1058, 1499, 1677, 2177
BsmBI CGTCTC 3 cut(s) 866, 1041, 1677
BsmFI GGGAC 3 cut(s) 797, 1813, 2160
BsmI GAATGC 1 cut(s) 2133
BsnI GGCC 6 cut(s) 5, 59, 969, 1727, 2092, 2111
Bso31I GGTCTC 1 cut(s) 1058
BsoBI CYCGRG 2 cut(s) 542, 2177
Bsp1286I GDGCHC 3 cut(s) 571, 860, 1639
Bsp143I GATC 4 cut(s) 151, 643, 1583, 1768
Bsp19I CCATGG 3 cut(s) 1543, 2112, 2150
BspACI CCGC 6 cut(s) 60, 107, 110, 966, 1280, 1589
BspANI GGCC 6 cut(s) 5, 59, 969, 1727, 2092, 2111
BspCNI CTCAG 1 cut(s) 2179
BspDI ATCGAT 1 cut(s) 945
BspFNI CGCG 3 cut(s) 638, 956, 1753
BspLI GGNNCC 6 cut(s) 268, 920, 1252, 1339, 1411, 1818
BspMAI CTGCAG 1 cut(s) 883
BspMI ACCTGC 1 cut(s) 872
BspOI GCTAGC 1 cut(s) 89
BspPI GGATC 1 cut(s) 1591
BspT107I GGYRCC 2 cut(s) 266, 1409
BspTNI GGTCTC 1 cut(s) 1058
BsrFI RCCGGY 2 cut(s) 554, 894
BsrI ACTGG 2 cut(s) 390, 653
BssAI RCCGGY 2 cut(s) 554, 894
BssECI CCNNGG 7 cut(s) 436, 928, 1254, 1543, 2112, 2150, 2178
BssMI GATC 4 cut(s) 151, 643, 1583, 1768
BssNI GRCGYC 2 cut(s) 1410, 1860
BssSI CACGAG 1 cut(s) 1015
BssT1I CCWWGG 4 cut(s) 436, 1543, 2112, 2150
Bst2BI CACGAG 1 cut(s) 1015
Bst2UI CCWGG 3 cut(s) 906, 1005, 1069
Bst4CI ACNGT 5 cut(s) 238, 862, 974, 1211, 1351
BstACI GRCGYC 2 cut(s) 1410, 1860
BstAPI GCANNNNNTGC 1 cut(s) 328
BstC8I GCNNGC 5 cut(s) 87, 396, 902, 2195, 2291
BstDEI CTNAG 1 cut(s) 2187
BstDSI CCRYGG 4 cut(s) 1254, 1543, 2112, 2150
BstEII GGTNACC 1 cut(s) 2202
BstF5I GGATG 2 cut(s) 579, 2146
BstFNI CGCG 3 cut(s) 638, 956, 1753
BstH2I RGCGCY 1 cut(s) 1413
BstHHI GCGC 3 cut(s) 97, 956, 1412
BstKTI GATC 4 cut(s) 154, 646, 1586, 1771
BstMAI GTCTC 6 cut(s) 866, 1041, 1058, 1499, 1677, 2177
BstMBI GATC 4 cut(s) 151, 643, 1583, 1768
BstMCI CGRYCG 2 cut(s) 203, 853
BstMWI GCNNNNNNNGC 4 cut(s) 328, 906, 1801, 2054
BstNI CCWGG 3 cut(s) 906, 1005, 1069
BstNSI RCATGY 1 cut(s) 2293
BstPI GGTNACC 1 cut(s) 2202
BstSCI CCNGG 3 cut(s) 904, 1003, 1067
BstSFI CTRYAG 2 cut(s) 879, 1864
BstSLI GKGCMC 2 cut(s) 571, 1639
BstUI CGCG 3 cut(s) 638, 956, 1753
BstV2I GAAGAC 1 cut(s) 486
BstXI CCANNNNNNTGG 2 cut(s) 613, 2119
Bsu15I ATCGAT 1 cut(s) 945
BsuI GTATCC 1 cut(s) 250
BsuRI GGCC 6 cut(s) 5, 59, 969, 1727, 2092, 2111
BsuTUI ATCGAT 1 cut(s) 945
BtgI CCRYGG 4 cut(s) 1254, 1543, 2112, 2150
BtgZI GCGATG 1 cut(s) 199
BtrI CACGTC 1 cut(s) 1687
BtsCI GGATG 2 cut(s) 579, 2146
BtsI GCAGTG 1 cut(s) 80
BtsIMutI CAGTG 5 cut(s) 80, 234, 383, 646, 970
BveI ACCTGC 1 cut(s) 872
Cac8I GCNNGC 5 cut(s) 87, 396, 902, 2195, 2291
CfoI GCGC 3 cut(s) 97, 956, 1412
Cfr10I RCCGGY 2 cut(s) 554, 894
Cfr13I GGNCC 1 cut(s) 1816
ClaI ATCGAT 1 cut(s) 945
CseI GACGC 3 cut(s) 1810, 1940, 2009
CsiI ACCWGGT 1 cut(s) 1067
Csp6I GTAC 4 cut(s) 267, 1195, 1534, 2268
CviQI GTAC 4 cut(s) 267, 1195, 1534, 2268
DdeI CTNAG 1 cut(s) 2187
DinI GGCGCC 1 cut(s) 1411
DpnI GATC 4 cut(s) 153, 645, 1585, 1770
DpnII GATC 4 cut(s) 151, 643, 1583, 1768
EaeI YGGCCR 4 cut(s) 57, 967, 1725, 2109
Eco130I CCWWGG 4 cut(s) 436, 1543, 2112, 2150
Eco147I AGGCCT 1 cut(s) 2092
Eco31I GGTCTC 1 cut(s) 1058
Eco32I GATATC 1 cut(s) 943
Eco47I GGWCC 1 cut(s) 1816
Eco57I CTGAAG 1 cut(s) 1530
Eco88I CYCGRG 2 cut(s) 542, 2177
Eco91I GGTNACC 1 cut(s) 2202
EcoO65I GGTNACC 1 cut(s) 2202
EcoRI GAATTC 3 cut(s) 206, 1052, 1362
EcoRII CCWGG 3 cut(s) 904, 1003, 1067
EcoRV GATATC 1 cut(s) 943
EcoT14I CCWWGG 4 cut(s) 436, 1543, 2112, 2150
EgeI GGCGCC 1 cut(s) 1411
EheI GGCGCC 1 cut(s) 1411
ErhI CCWWGG 4 cut(s) 436, 1543, 2112, 2150
Esp3I CGTCTC 3 cut(s) 866, 1041, 1677
FalI AAGNNNNNCTT 4 cut(s) 1181, 1213, 1788, 1820
FaqI GGGAC 3 cut(s) 797, 1813, 2160
FauNDI CATATG 1 cut(s) 2314
FbaI TGATCA 1 cut(s) 643
FblI GTMKAC 2 cut(s) 849, 1863
FokI GGATG 2 cut(s) 566, 2133
GlaI GCGC 3 cut(s) 96, 955, 1411
GsaI CCCAGC 1 cut(s) 2201
GsuI CTGGAG 2 cut(s) 407, 987
HaeII RGCGCY 1 cut(s) 1413
HaeIII GGCC 6 cut(s) 5, 59, 969, 1727, 2092, 2111
HapII CCGG 5 cut(s) 203, 555, 758, 853, 895
HgaI GACGC 3 cut(s) 1810, 1940, 2009
HhaI GCGC 3 cut(s) 97, 956, 1412
Hin1I GRCGYC 2 cut(s) 1410, 1860
Hin6I GCGC 3 cut(s) 95, 954, 1410
HinP1I GCGC 3 cut(s) 95, 954, 1410
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 1 cut(s) 2220
HinfI GANTC 5 cut(s) 22, 482, 545, 1035, 1324
HpaII CCGG 5 cut(s) 203, 555, 758, 853, 895
Hpy166II GTNNAC 5 cut(s) 383, 590, 850, 1354, 1864
Hpy188I TCNGA 6 cut(s) 628, 978, 1040, 1624, 1738, 2218
Hpy188III TCNNGA 5 cut(s) 1504, 1682, 1766, 1997, 2177
Hpy8I GTNNAC 5 cut(s) 383, 590, 850, 1354, 1864
HpyCH4III ACNGT 5 cut(s) 238, 862, 974, 1211, 1351
HpyCH4IV ACGT 3 cut(s) 447, 1686, 1860
HpyF10VI GCNNNNNNNGC 4 cut(s) 328, 906, 1801, 2054
HpyF3I CTNAG 1 cut(s) 2187
HpySE526I ACGT 3 cut(s) 447, 1686, 1860
Hsp92I GRCGYC 2 cut(s) 1410, 1860
HspAI GCGC 3 cut(s) 95, 954, 1410
KasI GGCGCC 1 cut(s) 1409
KpnI GGTACC 1 cut(s) 270
Ksp22I TGATCA 1 cut(s) 643
Kzo9I GATC 4 cut(s) 151, 643, 1583, 1768
LmnI GCTCC 6 cut(s) 388, 403, 855, 1250, 1337, 1745
LweI GCATC 4 cut(s) 662, 1628, 1931, 2155
MabI ACCWGGT 1 cut(s) 1067
MaeII ACGT 3 cut(s) 447, 1686, 1860
MaeIII GTNAC 6 cut(s) 28, 221, 862, 884, 1856, 2202
MalI GATC 4 cut(s) 153, 645, 1585, 1770
MboI GATC 4 cut(s) 151, 643, 1583, 1768
MboII GAAGA 6 cut(s) 491, 701, 817, 1295, 1624, 1956
MhlI GDGCHC 3 cut(s) 571, 860, 1639
MlsI TGGCCA 1 cut(s) 2111
MluNI TGGCCA 1 cut(s) 2111
Mly113I GGCGCC 1 cut(s) 1410
MlyI GAGTC 2 cut(s) 476, 554
MmeI TCCRAC 2 cut(s) 651, 2100
Mox20I TGGCCA 1 cut(s) 2111
MroXI GAANNNNTTC 2 cut(s) 1196, 1827
MscI TGGCCA 1 cut(s) 2111
MseI TTAA 8 cut(s) 47, 768, 1136, 1217, 1437, 1895, 2238, 2306
MslI CAYNNNNRTG 4 cut(s) 346, 611, 1317, 1473
Msp20I TGGCCA 1 cut(s) 2111
MspA1I CMGCKG 1 cut(s) 1280
MspI CCGG 5 cut(s) 203, 555, 758, 853, 895
MspR9I CCNGG 3 cut(s) 906, 1005, 1069
Mva1269I GAATGC 1 cut(s) 2133
MvaI CCWGG 3 cut(s) 906, 1005, 1069
MvnI CGCG 3 cut(s) 638, 956, 1753
MwoI GCNNNNNNNGC 4 cut(s) 328, 906, 1801, 2054
NarI GGCGCC 1 cut(s) 1410
NcoI CCATGG 3 cut(s) 1543, 2112, 2150
NdeI CATATG 1 cut(s) 2314
NdeII GATC 4 cut(s) 151, 643, 1583, 1768
NheI GCTAGC 1 cut(s) 85
NlaIV GGNNCC 6 cut(s) 268, 920, 1252, 1339, 1411, 1818
NmuCI GTSAC 4 cut(s) 221, 862, 1856, 2202
NspI RCATGY 1 cut(s) 2293
PaeI GCATGC 1 cut(s) 2293
PceI AGGCCT 1 cut(s) 2092
PcsI WCGNNNNNNNCGW 1 cut(s) 681
PctI GAATGC 1 cut(s) 2133
PdmI GAANNNNTTC 2 cut(s) 1196, 1827
PfeI GAWTC 3 cut(s) 22, 1035, 1324
PflFI GACNNNGTC 1 cut(s) 1069
PfoI TCCNGGA 1 cut(s) 1003
PleI GAGTC 2 cut(s) 476, 553
PluTI GGCGCC 1 cut(s) 1413
PpsI GAGTC 2 cut(s) 476, 553
Psp1406I AACGTT 1 cut(s) 447
Psp6I CCWGG 3 cut(s) 904, 1003, 1067
PspEI GGTNACC 1 cut(s) 2202
PspFI CCCAGC 1 cut(s) 2197
PspGI CCWGG 3 cut(s) 904, 1003, 1067
PspN4I GGNNCC 6 cut(s) 268, 920, 1252, 1339, 1411, 1818
PspPI GGNCC 1 cut(s) 1816
PstI CTGCAG 1 cut(s) 883
PsyI GACNNNGTC 1 cut(s) 1069
RsaI GTAC 4 cut(s) 268, 1196, 1535, 2269
RsaNI GTAC 4 cut(s) 267, 1195, 1534, 2268
RseI CAYNNNNRTG 4 cut(s) 346, 611, 1317, 1473
SalI GTCGAC 1 cut(s) 848
SaqAI TTAA 8 cut(s) 47, 768, 1136, 1217, 1437, 1895, 2238, 2306
Sau3AI GATC 4 cut(s) 151, 643, 1583, 1768
Sau96I GGNCC 1 cut(s) 1816
ScaI AGTACT 1 cut(s) 1196
SchI GAGTC 2 cut(s) 476, 554
ScrFI CCNGG 3 cut(s) 906, 1005, 1069
SduI GDGCHC 3 cut(s) 571, 860, 1639
SexAI ACCWGGT 1 cut(s) 1067
SfaNI GCATC 4 cut(s) 662, 1628, 1931, 2155
SfcI CTRYAG 2 cut(s) 879, 1864
SfoI GGCGCC 1 cut(s) 1411
SinI GGWCC 1 cut(s) 1816
SmiMI CAYNNNNRTG 4 cut(s) 346, 611, 1317, 1473
SmlI CTYRAG 3 cut(s) 117, 961, 1289
SmoI CTYRAG 3 cut(s) 117, 961, 1289
SpeI ACTAGT 3 cut(s) 1522, 1789, 2273
SphI GCATGC 1 cut(s) 2293
SseBI AGGCCT 1 cut(s) 2092
SsiI CCGC 6 cut(s) 60, 107, 110, 966, 1280, 1589
SspDI GGCGCC 1 cut(s) 1409
SspI AATATT 1 cut(s) 523
StuI AGGCCT 1 cut(s) 2092
StyD4I CCNGG 3 cut(s) 904, 1003, 1067
StyI CCWWGG 4 cut(s) 436, 1543, 2112, 2150
TaaI ACNGT 5 cut(s) 238, 862, 974, 1211, 1351
TaiI ACGT 3 cut(s) 450, 1689, 1863
TaqI TCGA 4 cut(s) 849, 945, 1503, 1681
TatI WGTACW 2 cut(s) 1194, 1533
TauI GCSGC 3 cut(s) 62, 112, 969
TfiI GAWTC 3 cut(s) 22, 1035, 1324
Tru1I TTAA 8 cut(s) 47, 768, 1136, 1217, 1437, 1895, 2238, 2306
Tru9I TTAA 8 cut(s) 47, 768, 1136, 1217, 1437, 1895, 2238, 2306
TscAI CASTG 5 cut(s) 87, 241, 390, 653, 977
TseFI GTSAC 4 cut(s) 221, 862, 1856, 2202
Tsp45I GTSAC 4 cut(s) 221, 862, 1856, 2202
TspDTI ATGAA 9 cut(s) 492, 670, 1175, 1584, 1747, 1820, 2114, 2160, 2268
TspGWI ACGGA 1 cut(s) 996
TspRI CASTG 5 cut(s) 87, 241, 390, 653, 977
Tth111I GACNNNGTC 1 cut(s) 1069
VpaK11BI GGWCC 1 cut(s) 1816
XapI RAATTY 8 cut(s) 206, 296, 994, 1052, 1362, 1992, 2167, 2295
XbaI TCTAGA 1 cut(s) 1765
XceI RCATGY 1 cut(s) 2293
XmiI GTMKAC 2 cut(s) 849, 1863
XmnI GAANNNNTTC 2 cut(s) 1196, 1827
ZraI GACGTC 1 cut(s) 1861
ZrmI AGTACT 1 cut(s) 1196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.