Rh5AG218000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0023005
Physical Location & Seq
Reverse (-)
25691624 .. 25692052
429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG218000.1

Sequence Viewer

Length: 429 bp
ATGATGCTGCAATGGACGCAACTAGTGTCTCGGATGTGTTGCTGCTTCCCCATGCTGCAGGAACTTGTGCGAGTGTACCTTAGAGTTAGAGGGATTCAAGTGAGTATCAACAGAGAAGAAGAAGAGCCGGTGGTTGCTTATGGTGCATCTCTTGTAGGTGCAATGTTGTGCACAGCTAGTATAAAGGAGGTGCAGTCTCTATCGATTTGTCATGCAACTACACTATCCGTGAGTCATGTGACATTCAGCCCGATAATACAACTACTTGCCCTACTATCCCCAAAACAAAAAAAGTTGGAGGTCCTGAATAAGAAGACAGTTATTCCAAGGAAGACTCCCATTCCAGCAATTAACAAGAGTACAAGACAGGTATTAACTTTTGGTTCCCTGAAGAGGTCACCAGAAAAGAAGCATGATGACTCTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

142

Amino Acids

15.92

Weight (kDa)

9.63

Isoelectric Point (pI)

53.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0023005)

Species Orthologous Gene IDs
rosa_laevigata RLG00000033305
rosa_multiflora Rmu_sc0014478.1_g000007
rosa_rugosa Rorug05G0125200
rosa_samantha Rh5AG218000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 410
AfaI GTAC 2 cut(s) 77, 361
AfiI CCNNNNNNNGG 1 cut(s) 393
AgsI TTSAA 1 cut(s) 98
AhlI ACTAGT 1 cut(s) 22
AluBI AGCT 1 cut(s) 176
AluI AGCT 1 cut(s) 176
Alw21I GWGCWC 1 cut(s) 173
Alw26I GTCTC 2 cut(s) 33, 201
Alw44I GTGCAC 1 cut(s) 169
ApaLI GTGCAC 1 cut(s) 169
ApeKI GCWGC 3 cut(s) 7, 42, 55
AspS9I GGNCC 1 cut(s) 301
AsuHPI GGTGA 1 cut(s) 390
AvaII GGWCC 1 cut(s) 301
BaeGI GKGCMC 1 cut(s) 173
BbsI GAAGAC 2 cut(s) 320, 338
Bbv12I GWGCWC 1 cut(s) 173
BbvI GCAGC 2 cut(s) 29, 42
BcoDI GTCTC 2 cut(s) 33, 201
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 2 cut(s) 23, 177
BfmI CTRYAG 1 cut(s) 56
BisI GCNGC 3 cut(s) 8, 43, 56
BlsI GCNGC 3 cut(s) 9, 44, 57
Bme18I GGWCC 1 cut(s) 301
BmgT120I GGNCC 1 cut(s) 301
BmiI GGNNCC 1 cut(s) 385
BmsI GCATC 1 cut(s) 155
BpiI GAAGAC 2 cut(s) 320, 338
Bsa29I ATCGAT 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 326
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 1 cut(s) 393
Bse118I RCCGGY 1 cut(s) 127
Bse3DI GCAATG 2 cut(s) 17, 168
BseCI ATCGAT 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 326
BseGI GGATG 1 cut(s) 39
BseLI CCNNNNNNNGG 1 cut(s) 393
BseMI GCAATG 2 cut(s) 17, 168
BseSI GKGCMC 1 cut(s) 173
BseXI GCAGC 2 cut(s) 29, 42
BsgI GTGCAG 1 cut(s) 212
BshVI ATCGAT 1 cut(s) 203
BsiHKAI GWGCWC 1 cut(s) 173
BsiSI CCGG 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 393
BsmAI GTCTC 2 cut(s) 33, 201
Bsp1286I GDGCHC 1 cut(s) 173
BspDI ATCGAT 1 cut(s) 203
BspLI GGNNCC 1 cut(s) 385
BspMAI CTGCAG 1 cut(s) 60
BspQI GCTCTTC 1 cut(s) 117
BsrDI GCAATG 2 cut(s) 17, 168
BsrFI RCCGGY 1 cut(s) 127
BssAI RCCGGY 1 cut(s) 127
BssECI CCNNGG 1 cut(s) 326
BssT1I CCWWGG 1 cut(s) 326
Bst4CI ACNGT 1 cut(s) 319
Bst6I CTCTTC 2 cut(s) 117, 386
BstDEI CTNAG 1 cut(s) 80
BstEII GGTNACC 1 cut(s) 396
BstF5I GGATG 1 cut(s) 39
BstMAI GTCTC 2 cut(s) 33, 201
BstMWI GCNNNNNNNGC 2 cut(s) 16, 143
BstPI GGTNACC 1 cut(s) 396
BstSFI CTRYAG 1 cut(s) 56
BstSLI GKGCMC 1 cut(s) 173
BstV1I GCAGC 2 cut(s) 29, 42
BstV2I GAAGAC 2 cut(s) 320, 338
Bsu15I ATCGAT 1 cut(s) 203
BsuTUI ATCGAT 1 cut(s) 203
BtsCI GGATG 1 cut(s) 39
Cfr10I RCCGGY 1 cut(s) 127
Cfr13I GGNCC 1 cut(s) 301
ClaI ATCGAT 1 cut(s) 203
CseI GACGC 1 cut(s) 25
Csp6I GTAC 2 cut(s) 76, 360
CviAII CATG 4 cut(s) 52, 212, 236, 413
CviJI RGCY 3 cut(s) 127, 176, 249
CviKI_1 RGCY 3 cut(s) 127, 176, 249
CviQI GTAC 2 cut(s) 76, 360
DdeI CTNAG 1 cut(s) 80
Eam1104I CTCTTC 2 cut(s) 117, 386
EarI CTCTTC 2 cut(s) 117, 386
Eco130I CCWWGG 1 cut(s) 326
Eco47I GGWCC 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 410
Eco91I GGTNACC 1 cut(s) 396
EcoO109I RGGNCCY 1 cut(s) 301
EcoO65I GGTNACC 1 cut(s) 396
EcoT14I CCWWGG 1 cut(s) 326
ErhI CCWWGG 1 cut(s) 326
FaeI CATG 4 cut(s) 55, 215, 239, 416
FaiI YATR 7 cut(s) 53, 141, 182, 213, 237, 414, 427
FatI CATG 4 cut(s) 51, 211, 235, 412
Fnu4HI GCNGC 3 cut(s) 8, 43, 56
FokI GGATG 1 cut(s) 46
Fsp4HI GCNGC 3 cut(s) 8, 43, 56
FspBI CTAG 2 cut(s) 23, 177
GluI GCNGC 3 cut(s) 8, 43, 56
HapII CCGG 1 cut(s) 128
HgaI GACGC 1 cut(s) 25
Hin1II CATG 4 cut(s) 55, 215, 239, 416
HinfI GANTC 4 cut(s) 94, 232, 334, 419
HpaII CCGG 1 cut(s) 128
HphI GGTGA 1 cut(s) 390
Hpy166II GTNNAC 2 cut(s) 76, 171
Hpy188I TCNGA 1 cut(s) 33
Hpy188III TCNNGA 1 cut(s) 304
Hpy8I GTNNAC 2 cut(s) 76, 171
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4V TGCA 7 cut(s) 10, 58, 146, 161, 171, 193, 215
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 143
HpyF3I CTNAG 1 cut(s) 80
Hsp92II CATG 4 cut(s) 55, 215, 239, 416
LguI GCTCTTC 1 cut(s) 117
LpnPI CCDG 7 cut(s) 44, 141, 317, 353, 357, 401, 414
Lsp1109I GCAGC 2 cut(s) 29, 42
LweI GCATC 1 cut(s) 155
MaeI CTAG 2 cut(s) 23, 177
MaeIII GTNAC 2 cut(s) 238, 396
MboII GAAGA 6 cut(s) 128, 131, 134, 325, 343, 403
MhlI GDGCHC 1 cut(s) 173
MluCI AATT 1 cut(s) 348
MlyI GAGTC 3 cut(s) 241, 328, 413
MmeI TCCRAC 1 cut(s) 276
MnlI CCTC 4 cut(s) 83, 181, 292, 387
MseI TTAA 2 cut(s) 351, 374
MspI CCGG 1 cut(s) 128
MwoI GCNNNNNNNGC 2 cut(s) 16, 143
NlaIII CATG 4 cut(s) 55, 215, 239, 416
NlaIV GGNNCC 1 cut(s) 385
NmuCI GTSAC 2 cut(s) 238, 396
PciSI GCTCTTC 1 cut(s) 117
PfeI GAWTC 1 cut(s) 94
PkrI GCNGC 3 cut(s) 9, 44, 57
PleI GAGTC 3 cut(s) 240, 328, 413
PpsI GAGTC 3 cut(s) 240, 328, 413
PpuMI RGGWCCY 1 cut(s) 301
Psp5II RGGWCCY 1 cut(s) 301
PspEI GGTNACC 1 cut(s) 396
PspN4I GGNNCC 1 cut(s) 385
PspPI GGNCC 1 cut(s) 301
PspPPI RGGWCCY 1 cut(s) 301
PstI CTGCAG 1 cut(s) 60
RsaI GTAC 2 cut(s) 77, 361
RsaNI GTAC 2 cut(s) 76, 360
SapI GCTCTTC 1 cut(s) 117
SaqAI TTAA 2 cut(s) 351, 374
SatI GCNGC 3 cut(s) 8, 43, 56
Sau96I GGNCC 1 cut(s) 301
SchI GAGTC 3 cut(s) 241, 328, 413
SduI GDGCHC 1 cut(s) 173
SetI ASST 7 cut(s) 81, 160, 178, 192, 303, 372, 398
SfaNI GCATC 1 cut(s) 155
SfcI CTRYAG 1 cut(s) 56
SinI GGWCC 1 cut(s) 301
SpeI ACTAGT 1 cut(s) 22
Sse9I AATT 1 cut(s) 348
SspMI CTAG 2 cut(s) 23, 177
StyI CCWWGG 1 cut(s) 326
TaaI ACNGT 1 cut(s) 319
TaqI TCGA 1 cut(s) 203
TasI AATT 1 cut(s) 348
TatI WGTACW 1 cut(s) 359
TfiI GAWTC 1 cut(s) 94
Tru1I TTAA 2 cut(s) 351, 374
Tru9I TTAA 2 cut(s) 351, 374
TseFI GTSAC 2 cut(s) 238, 396
TseI GCWGC 3 cut(s) 7, 42, 55
Tsp45I GTSAC 2 cut(s) 238, 396
TspGWI ACGGA 1 cut(s) 217
VneI GTGCAC 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 301
XspI CTAG 2 cut(s) 23, 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.