Rorug05G0133700

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004374
Physical Location & Seq
Reverse (-)
11938311 .. 11939593
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0133700.1

Sequence Viewer

Length: 570 bp
ATGGCGCTAGAAAACGGAGAGAAGCCAGGGTCTAGTTTTGTGGCAGTGAGAAAACCAAATGATTGTACCATTCTCTTGCTGAGAGTGGTTGCATTCTTGGCCACACTCGCCGCGACTCTAGTGATGGCACTCAACAAAGAAACCAAAACCTTGGTTGTTGCCACTATTGGGACTACTCCGATTAAAGCCACGCTCACAGCCAAGTTTCAACACACTCCAGCAAATGTGTTCTTTGTTATAGCTAATGCAATGGCCAGCCTTCACAACTTGTTGATGATAATAGTGCAACTTTGCGGAGGCAAGTTCAAAGACAAGGGTCTTCGCCTTGTGATGATGGCCATTTTAGACATGATGACTGTGGCCCTGCTATCTTCTGGCGATGGTGCAGCTACCTTCATGGCACATCTAGCGAAGAAAGGTAATTCACATGCAAGGTGGAACAAAATCTGTGACAAGTTTGGCAAGTTCTGCGACCATGGTGGTGGCGCTATCATTGCTTCTTATATTGGGCTCCTTCTCTTGGTGGTTATCACAGTGTTGTCCATCATCAAGCTACTCAAGGCGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

189

Amino Acids

20.15

Weight (kDa)

9.85

Isoelectric Point (pI)

22.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 21 - 172 2e-38 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 113
AciI CCGC 2 cut(s) 111, 294
AcoI YGGCCR 3 cut(s) 99, 252, 336
AfaI GTAC 1 cut(s) 67
AfiI CCNNNNNNNGG 2 cut(s) 168, 520
AgsI TTSAA 2 cut(s) 209, 307
AjnI CCWGG 1 cut(s) 25
AluBI AGCT 3 cut(s) 242, 389, 553
AluI AGCT 3 cut(s) 242, 389, 553
AoxI GGCC 4 cut(s) 99, 252, 336, 360
ApeKI GCWGC 1 cut(s) 386
AspLEI GCGC 2 cut(s) 7, 488
AspS9I GGNCC 1 cut(s) 361
BalI TGGCCA 3 cut(s) 101, 254, 338
BanII GRGCYC 1 cut(s) 513
BbsI GAAGAC 1 cut(s) 311
BbvI GCAGC 1 cut(s) 398
BccI CCATC 4 cut(s) 118, 328, 374, 551
BciT130I CCWGG 1 cut(s) 27
BfaI CTAG 4 cut(s) 8, 33, 119, 407
BfoI RGCGCY 2 cut(s) 8, 489
BisI GCNGC 2 cut(s) 111, 387
BlsI GCNGC 2 cut(s) 112, 388
Bme1390I CCNGG 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 361
BmiI GGNNCC 1 cut(s) 512
BmrFI CCNGG 1 cut(s) 27
BoxI GACNNNNGTC 1 cut(s) 315
BpiI GAAGAC 1 cut(s) 311
BpmI CTGGAG 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 542
BsaJI CCNNGG 3 cut(s) 26, 150, 475
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 520
Bse3DI GCAATG 2 cut(s) 255, 492
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 3 cut(s) 26, 150, 475
BseLI CCNNNNNNNGG 2 cut(s) 168, 520
BseMI GCAATG 2 cut(s) 255, 492
BseMII CTCAG 1 cut(s) 71
BseXI GCAGC 1 cut(s) 398
BsgI GTGCAG 1 cut(s) 405
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 4 cut(s) 101, 254, 338, 362
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 2 cut(s) 168, 520
BsmFI GGGAC 1 cut(s) 184
BsmI GAATGC 1 cut(s) 92
BsnI GGCC 4 cut(s) 101, 254, 338, 362
Bsp1286I GDGCHC 1 cut(s) 513
Bsp19I CCATGG 1 cut(s) 475
BspACI CCGC 2 cut(s) 111, 294
BspANI GGCC 4 cut(s) 101, 254, 338, 362
BspCNI CTCAG 1 cut(s) 72
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 1 cut(s) 512
BsrDI GCAATG 2 cut(s) 255, 492
BssECI CCNNGG 3 cut(s) 26, 150, 475
BssT1I CCWWGG 2 cut(s) 150, 475
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 2 cut(s) 358, 535
BstAPI GCANNNNNTGC 1 cut(s) 468
BstC8I GCNNGC 1 cut(s) 256
BstDEI CTNAG 1 cut(s) 80
BstDSI CCRYGG 1 cut(s) 475
BstFNI CGCG 1 cut(s) 113
BstH2I RGCGCY 2 cut(s) 8, 489
BstHHI GCGC 2 cut(s) 7, 488
BstMWI GCNNNNNNNGC 5 cut(s) 98, 107, 407, 468, 494
BstNI CCWGG 1 cut(s) 27
BstNSI RCATGY 1 cut(s) 431
BstPAI GACNNNNGTC 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 25
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 1 cut(s) 398
BstV2I GAAGAC 1 cut(s) 311
BstXI CCANNNNNNTGG 2 cut(s) 151, 482
BsuRI GGCC 4 cut(s) 101, 254, 338, 362
BtgI CCRYGG 1 cut(s) 475
BtgZI GCGATG 1 cut(s) 393
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 2 cut(s) 51, 540
Cac8I GCNNGC 1 cut(s) 256
CfoI GCGC 2 cut(s) 7, 488
Cfr13I GGNCC 1 cut(s) 361
Csp6I GTAC 1 cut(s) 66
CviAII CATG 4 cut(s) 349, 397, 428, 476
CviQI GTAC 1 cut(s) 66
DdeI CTNAG 1 cut(s) 80
EaeI YGGCCR 3 cut(s) 99, 252, 336
Eco130I CCWWGG 2 cut(s) 150, 475
Eco24I GRGCYC 1 cut(s) 513
EcoRII CCWGG 1 cut(s) 25
EcoT14I CCWWGG 2 cut(s) 150, 475
EcoT38I GRGCYC 1 cut(s) 513
ErhI CCWWGG 2 cut(s) 150, 475
FaeI CATG 4 cut(s) 352, 400, 431, 479
FaiI YATR 6 cut(s) 239, 350, 398, 429, 477, 504
FaqI GGGAC 1 cut(s) 184
FatI CATG 4 cut(s) 348, 396, 427, 475
Fnu4HI GCNGC 2 cut(s) 111, 387
FriOI GRGCYC 1 cut(s) 513
Fsp4HI GCNGC 2 cut(s) 111, 387
FspBI CTAG 4 cut(s) 8, 33, 119, 407
GlaI GCGC 2 cut(s) 6, 487
GluI GCNGC 2 cut(s) 111, 387
GsuI CTGGAG 1 cut(s) 201
HaeII RGCGCY 2 cut(s) 8, 489
HaeIII GGCC 4 cut(s) 101, 254, 338, 362
HhaI GCGC 2 cut(s) 7, 488
Hin1II CATG 4 cut(s) 352, 400, 431, 479
Hin6I GCGC 2 cut(s) 5, 486
HinP1I GCGC 2 cut(s) 5, 486
HinfI GANTC 1 cut(s) 115
Hpy188I TCNGA 1 cut(s) 180
HpyAV CCTTC 3 cut(s) 269, 403, 524
HpyCH4III ACNGT 2 cut(s) 358, 535
HpyCH4V TGCA 5 cut(s) 92, 248, 286, 386, 431
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 107, 407, 468, 494
HpyF3I CTNAG 1 cut(s) 80
Hsp92II CATG 4 cut(s) 352, 400, 431, 479
HspAI GCGC 2 cut(s) 5, 486
LmnI GCTCC 1 cut(s) 516
LpnPI CCDG 6 cut(s) 12, 39, 231, 268, 360, 377
Lsp1109I GCAGC 1 cut(s) 398
MaeI CTAG 4 cut(s) 8, 33, 119, 407
MaeIII GTNAC 1 cut(s) 449
MboII GAAGA 3 cut(s) 311, 363, 424
MhlI GDGCHC 1 cut(s) 513
MlsI TGGCCA 3 cut(s) 101, 254, 338
MluCI AATT 1 cut(s) 421
MluNI TGGCCA 3 cut(s) 101, 254, 338
MlyI GAGTC 1 cut(s) 109
MnlI CCTC 1 cut(s) 290
Mox20I TGGCCA 3 cut(s) 101, 254, 338
MscI TGGCCA 3 cut(s) 101, 254, 338
MseI TTAA 1 cut(s) 183
MslI CAYNNNNRTG 1 cut(s) 480
Msp20I TGGCCA 3 cut(s) 101, 254, 338
MspR9I CCNGG 1 cut(s) 27
Mva1269I GAATGC 1 cut(s) 92
MvaI CCWGG 1 cut(s) 27
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 5 cut(s) 98, 107, 407, 468, 494
NcoI CCATGG 1 cut(s) 475
NlaIII CATG 4 cut(s) 352, 400, 431, 479
NlaIV GGNNCC 1 cut(s) 512
NmuCI GTSAC 1 cut(s) 449
NspI RCATGY 1 cut(s) 431
PctI GAATGC 1 cut(s) 92
PkrI GCNGC 2 cut(s) 112, 388
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PshAI GACNNNNGTC 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 25
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 512
PspPI GGNCC 1 cut(s) 361
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
RseI CAYNNNNRTG 1 cut(s) 480
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 2 cut(s) 111, 387
Sau96I GGNCC 1 cut(s) 361
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 1 cut(s) 513
SetI ASST 7 cut(s) 152, 244, 391, 395, 421, 437, 555
SmiMI CAYNNNNRTG 1 cut(s) 480
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
Sse9I AATT 1 cut(s) 421
SsiI CCGC 2 cut(s) 111, 294
SspMI CTAG 4 cut(s) 8, 33, 119, 407
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 2 cut(s) 150, 475
TaaI ACNGT 2 cut(s) 358, 535
TasI AATT 1 cut(s) 421
TauI GCSGC 1 cut(s) 113
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TscAI CASTG 2 cut(s) 51, 540
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 1 cut(s) 386
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 1 cut(s) 385
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 2 cut(s) 51, 540
XceI RCATGY 1 cut(s) 431
XspI CTAG 4 cut(s) 8, 33, 119, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.