Rorug05G0268800

ribonuclease H protein At1g65750-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010714
Physical Location & Seq
Reverse (-)
30345918 .. 30348646
2729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0268800.1

Sequence Viewer

Length: 1698 bp
ATGTCTCTCAACGTGGTCAGTGTAGCTTCTCAAGCCCCTCCTACCCTGAATGCCTCTACTGATGCCGATAGTCGACGTTCGGCTAATTTCCATCCCAGCATTTGGGGAGATCAGTTTCTGTCTTATAACAACACTATGGAAACTAACATAAATGGTGAGCAACATGTCCAACAACTGAAGGAAGAAGTGAAGAGAATGCTAATGGCTCCGCCAGTTCAAACAAGTTTGGGAAAACTGGAGTTGATTGATGACATTCAACGCTTGGGTGTATCTTACCATTTTGAATACGAAATTGACCAAACTATGCAACAAATTCACCACAATTTTAATGGTTACTGTGATGATGATCTGCACACTTGTGCTCTTCGTTTTCGCTTGCTGAGGCAGCATGGTTACAATGTTTCATGCGATACGTTCAACAAGTTCAAGGACAGTGATGGAAAATTGAACAAATCGCTTCACGATGATGTAGTGGGACTACAAAGCTTGTATGAAGCCACACATCTTAGAGTACGAGGAGAGGATTTTCTAGAAGAAGCACTAACCTTCACCACCACACATCTAGCGTCGGCAGTGCTAGCAAACCGTTTAAGCCCCCCGCTTTCGAAACAAGTAAGGCATGCCTTGAATCAACCACTCCGGAAGGGCTTACCGAGGCTAGAAGCGAGGCACTACATGTCTCTCCACCAGGAACTACACGGATCACGTAACCAAATTTTGCTGAGTTTTGCAAAGCTGGATTTCAACCTGCTCCAACAATTGCATCAGAAAGAACTAAGTGATATTGCAAGGTGGTGGAAGGAGTTGGACTTTGCTAGCAAGCTACCTTTTGCAAGAGACAGAGTAATTGAGTGCTACTTTTGGATTTTGGGAGTCTACTTTGAGCCCAAGTATTACTTTGCCAGGAGGACACTAACCAAAGTTATTGCCATGACATCCATTATCGATGACATTTATGATGTGTATGGCACGCTTGAAGAACTAGACCTCTTTACTAGAGCAATTGAGAGGTGGGACATCTCCGCCATGGATGAACTACCGGAGTATATGAAAGTCTGTTACGAGGCACTGTTAGATGTCTATGCTGATACAGAACAACAGCTTGCAAGCGAGGGAAGATTATATCGTATCGATTACGCAAAAGAAGCGATGAAGAGACAAGTTAGGGCATACCATGCTGAAGCAAAATGGTTACACAAGAACTACACACCAACAATGGATGAATACATGGAAGTAGCCCTTGTCACTTCAGCCTACTCAATGCTAGCAACCACATCCTTTGTTGGAATGGGAGATATCGTTACAAAAGACTCCTTTGAGTGGATCTTCAGTGACCCTAAGATTGTAAAGGCTTCATCAGTAGTTTGCAGACTCATGGATGACATTGTATCCCACAAGTTTGAGCAAAAGAGAGGACATGTTGCCTCGGCTGTGGAATGTTACATGAAACAATACGGTGCGACAGAAGAACAAACCATAATTGAATTTCGTAAACAAGTGATTGATGCATGGAAGGACATAAATGAAGAGTGCCTCCACCCTACACCTCTTGAAATGCCGCTCTTAATGCGAGTTCTGAATCTCACACGCGTGATAGATGTAGTATACAAGTCTGAAGATGGCTACACTCATGCTGGAACCATTCTCAAAGATTTTGTAGCTTCTCTACTTATCGAACCTGTGCCTGTGCCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

565

Amino Acids

65.29

Weight (kDa)

5.63

Isoelectric Point (pI)

49.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 34 - 209 3.4e-59 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 267 - 506 1.3e-105 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 307 - 506 7.6e-63 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010714)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51500 AT3G21090
fragaria_vesca FvH4_3g28460 FvH4_3g28460 FvH4_3g28460
malus_domestica MD03G1172200.v1.1 MD11G1189900.v1.1
prunus_persica Prupe.4G254600_v2.0.a1 Prupe.4G254600_v2.0.a1
pyrus_communis pycom03g12930 pycom11g16700
rosa_chinensis RchiOBHm_Chr5g0053001
rosa_laevigata RLG00000034782
rosa_multiflora Rmu_sc0003030.1_g000007
rosa_roxburghii Rroxscaffold_1G00028800
rosa_rugosa Rorug05G0268800 Rorug05G0268900
rosa_samantha Rh5AG341200 Rh5CG378400
rosa_wichuraiana Rw5G032160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 126
Acc36I ACCTGC 1 cut(s) 756
AccB7I CCANNNNNTGG 1 cut(s) 102
AccBSI CCGCTC 1 cut(s) 1561
AccI GTMKAC 3 cut(s) 73, 876, 1605
AccII CGCG 1 cut(s) 1590
AccIII TCCGGA 1 cut(s) 639
AciI CCGC 4 cut(s) 209, 599, 1023, 1559
AclWI GGATC 2 cut(s) 709, 1331
AcsI RAATTY 3 cut(s) 312, 714, 1484
AcuI CTGAAG 5 cut(s) 197, 1200, 1233, 1312, 1635
AfaI GTAC 1 cut(s) 513
AfiI CCNNNNNNNGG 2 cut(s) 102, 1320
AflIII ACRYGT 4 cut(s) 163, 675, 1417, 1588
AjnI CCWGG 2 cut(s) 687, 902
AleI CACNNNNGTG 2 cut(s) 357, 1589
AluBI AGCT 6 cut(s) 26, 486, 736, 823, 1102, 1661
AluI AGCT 6 cut(s) 26, 486, 736, 823, 1102, 1661
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 4 cut(s) 9, 684, 831, 1150
AlwI GGATC 2 cut(s) 709, 1331
AlwNI CAGNNNCTG 1 cut(s) 118
Aor13HI TCCGGA 1 cut(s) 639
ApeKI GCWGC 1 cut(s) 385
ApoI RAATTY 3 cut(s) 312, 714, 1484
AsuHPI GGTGA 3 cut(s) 167, 308, 541
AsuII TTCGAA 1 cut(s) 605
AsuNHI GCTAGC 3 cut(s) 577, 815, 1264
BanII GRGCYC 1 cut(s) 888
Bbv12I GWGCWC 1 cut(s) 364
BbvCI CCTCAGC 1 cut(s) 380
BbvI GCAGC 1 cut(s) 397
BccI CCATC 3 cut(s) 99, 431, 1613
BciT130I CCWGG 2 cut(s) 689, 904
BciVI GTATCC 1 cut(s) 1399
BcoDI GTCTC 4 cut(s) 9, 684, 831, 1150
BfaI CTAG 8 cut(s) 530, 563, 578, 659, 816, 983, 996, 1265
BfuAI ACCTGC 1 cut(s) 756
BfuI GTATCC 1 cut(s) 1399
BisI GCNGC 2 cut(s) 386, 1559
BlsI GCNGC 2 cut(s) 387, 1560
Bme1390I CCNGG 2 cut(s) 689, 904
BmiI GGNNCC 2 cut(s) 207, 1639
BmrFI CCNGG 2 cut(s) 689, 904
BmsI GCATC 3 cut(s) 52, 772, 1495
BmtI GCTAGC 3 cut(s) 581, 819, 1268
BpmI CTGGAG 1 cut(s) 257
Bpu10I CCTNAGC 1 cut(s) 380
Bpu14I TTCGAA 1 cut(s) 605
BpuEI CTTGAG 1 cut(s) 15
Bsa29I ATCGAT 2 cut(s) 945, 1131
BsaAI YACGTR 1 cut(s) 707
BsaBI GATNNNNATC 1 cut(s) 345
BsaJI CCNNGG 3 cut(s) 653, 1026, 1425
BsaWI WCCGGW 2 cut(s) 639, 1039
BsaXI ACNNNNNCTCC 2 cut(s) 1284, 1314
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 1320
Bse1I ACTGG 2 cut(s) 212, 240
Bse8I GATNNNNATC 1 cut(s) 345
BseAI TCCGGA 1 cut(s) 639
BseBI CCWGG 2 cut(s) 689, 904
BseCI ATCGAT 2 cut(s) 945, 1131
BseDI CCNNGG 3 cut(s) 653, 1026, 1425
BseGI GGATG 6 cut(s) 91, 935, 1036, 1225, 1274, 1384
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 2 cut(s) 102, 1320
BseMII CTCAG 2 cut(s) 371, 713
BseNI ACTGG 2 cut(s) 212, 240
BseRI GAGGAG 1 cut(s) 531
BseXI GCAGC 1 cut(s) 397
BseYI CCCAGC 1 cut(s) 95
BsgI GTGCAG 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 1590
BshVI ATCGAT 2 cut(s) 945, 1131
BsiHKAI GWGCWC 1 cut(s) 364
BsiSI CCGG 2 cut(s) 640, 1040
BslFI GGGAC 2 cut(s) 489, 1028
BslI CCNNNNNNNGG 2 cut(s) 102, 1320
BsmAI GTCTC 4 cut(s) 9, 684, 831, 1150
BsmFI GGGAC 2 cut(s) 489, 1028
BsmI GAATGC 2 cut(s) 55, 201
Bsp119I TTCGAA 1 cut(s) 605
Bsp1286I GDGCHC 2 cut(s) 364, 888
Bsp13I TCCGGA 1 cut(s) 639
Bsp143I GATC 4 cut(s) 109, 346, 701, 1323
Bsp19I CCATGG 1 cut(s) 1026
BspACI CCGC 4 cut(s) 209, 599, 1023, 1559
BspCNI CTCAG 2 cut(s) 372, 714
BspDI ATCGAT 2 cut(s) 945, 1131
BspEI TCCGGA 1 cut(s) 639
BspFNI CGCG 1 cut(s) 1590
BspLI GGNNCC 2 cut(s) 207, 1639
BspMI ACCTGC 1 cut(s) 756
BspOI GCTAGC 3 cut(s) 581, 819, 1268
BspPI GGATC 2 cut(s) 709, 1331
BspQI GCTCTTC 1 cut(s) 369
BspT104I TTCGAA 1 cut(s) 605
BsrBI CCGCTC 1 cut(s) 1561
BsrI ACTGG 2 cut(s) 212, 240
BssECI CCNNGG 3 cut(s) 653, 1026, 1425
BssMI GATC 4 cut(s) 109, 346, 701, 1323
BssNAI GTATAC 1 cut(s) 1606
BssT1I CCWWGG 1 cut(s) 1026
Bst1107I GTATAC 1 cut(s) 1606
Bst2UI CCWGG 2 cut(s) 689, 904
Bst4CI ACNGT 5 cut(s) 338, 434, 587, 1071, 1457
Bst6I CTCTTC 4 cut(s) 185, 369, 1148, 1521
BstAPI GCANNNNNTGC 1 cut(s) 1175
BstBAI YACGTR 1 cut(s) 707
BstBI TTCGAA 1 cut(s) 605
BstC8I GCNNGC 9 cut(s) 377, 579, 621, 817, 821, 971, 1104, 1108, 1266
BstDEI CTNAG 5 cut(s) 380, 506, 722, 776, 1338
BstDSI CCRYGG 1 cut(s) 1026
BstF5I GGATG 6 cut(s) 91, 935, 1036, 1225, 1274, 1384
BstFNI CGCG 1 cut(s) 1590
BstKTI GATC 4 cut(s) 112, 349, 704, 1326
BstMAI GTCTC 4 cut(s) 9, 684, 831, 1150
BstMBI GATC 4 cut(s) 109, 346, 701, 1323
BstMWI GCNNNNNNNGC 6 cut(s) 32, 385, 578, 1145, 1175, 1567
BstNI CCWGG 2 cut(s) 689, 904
BstNSI RCATGY 4 cut(s) 167, 623, 679, 1421
BstSCI CCNGG 2 cut(s) 687, 902
BstUI CGCG 1 cut(s) 1590
BstV1I GCAGC 1 cut(s) 397
BstX2I RGATCY 1 cut(s) 1323
BstYI RGATCY 1 cut(s) 1323
BstZ17I GTATAC 1 cut(s) 1606
Bsu15I ATCGAT 2 cut(s) 945, 1131
BsuI GTATCC 1 cut(s) 1399
BsuTUI ATCGAT 2 cut(s) 945, 1131
BtgI CCRYGG 1 cut(s) 1026
BtgZI GCGATG 1 cut(s) 1163
BtsCI GGATG 6 cut(s) 91, 935, 1036, 1225, 1274, 1384
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 5 cut(s) 25, 439, 579, 1067, 1336
BveI ACCTGC 1 cut(s) 756
Cac8I GCNNGC 9 cut(s) 377, 579, 621, 817, 821, 971, 1104, 1108, 1266
CaiI CAGNNNCTG 1 cut(s) 118
ClaI ATCGAT 2 cut(s) 945, 1131
CseI GACGC 1 cut(s) 555
Csp6I GTAC 1 cut(s) 512
CspCI CAANNNNNGTGG 2 cut(s) 1261, 1296
CviQI GTAC 1 cut(s) 512
DdeI CTNAG 5 cut(s) 380, 506, 722, 776, 1338
DpnI GATC 4 cut(s) 111, 348, 703, 1325
DpnII GATC 4 cut(s) 109, 346, 701, 1323
Eam1104I CTCTTC 4 cut(s) 185, 369, 1148, 1521
EarI CTCTTC 4 cut(s) 185, 369, 1148, 1521
EciI GGCGGA 2 cut(s) 198, 1012
Eco130I CCWWGG 1 cut(s) 1026
Eco24I GRGCYC 1 cut(s) 888
Eco32I GATATC 1 cut(s) 1297
Eco57I CTGAAG 5 cut(s) 197, 1200, 1233, 1312, 1635
EcoRII CCWGG 2 cut(s) 687, 902
EcoRV GATATC 1 cut(s) 1297
EcoT14I CCWWGG 1 cut(s) 1026
EcoT22I ATGCAT 1 cut(s) 1510
EcoT38I GRGCYC 1 cut(s) 888
ErhI CCWWGG 1 cut(s) 1026
FalI AAGNNNNNCTT 4 cut(s) 881, 913, 1224, 1256
FaqI GGGAC 2 cut(s) 489, 1028
FauI CCCGC 1 cut(s) 606
FblI GTMKAC 3 cut(s) 73, 876, 1605
Fnu4HI GCNGC 2 cut(s) 386, 1559
FokI GGATG 6 cut(s) 78, 922, 1043, 1232, 1261, 1391
FriOI GRGCYC 1 cut(s) 888
Fsp4HI GCNGC 2 cut(s) 386, 1559
FspBI CTAG 8 cut(s) 530, 563, 578, 659, 816, 983, 996, 1265
GluI GCNGC 2 cut(s) 386, 1559
GsaI CCCAGC 1 cut(s) 99
GsuI CTGGAG 1 cut(s) 257
HapII CCGG 2 cut(s) 640, 1040
HgaI GACGC 1 cut(s) 555
HincII GTYRAC 1 cut(s) 74
HindII GTYRAC 1 cut(s) 74
HindIII AAGCTT 1 cut(s) 484
HinfI GANTC 5 cut(s) 628, 873, 1310, 1371, 1579
HpaII CCGG 2 cut(s) 640, 1040
HphI GGTGA 3 cut(s) 167, 308, 541
Hpy166II GTNNAC 4 cut(s) 74, 877, 1493, 1606
Hpy188I TCNGA 3 cut(s) 768, 1578, 1615
Hpy188III TCNNGA 4 cut(s) 461, 530, 640, 1550
Hpy8I GTNNAC 4 cut(s) 74, 877, 1493, 1606
Hpy99I CGWCG 2 cut(s) 78, 571
HpyAV CCTTC 5 cut(s) 172, 556, 637, 793, 1507
HpyCH4III ACNGT 5 cut(s) 338, 434, 587, 1071, 1457
HpyCH4IV ACGT 4 cut(s) 12, 76, 413, 706
HpyCH4V TGCA 9 cut(s) 307, 352, 731, 763, 788, 833, 1106, 1368, 1508
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 385, 578, 1145, 1175, 1567
HpyF3I CTNAG 5 cut(s) 380, 506, 722, 776, 1338
HpySE526I ACGT 4 cut(s) 12, 76, 413, 706
Kpn2I TCCGGA 1 cut(s) 639
Kzo9I GATC 4 cut(s) 109, 346, 701, 1323
LguI GCTCTTC 1 cut(s) 369
LmnI GCTCC 2 cut(s) 211, 756
Lsp1109I GCAGC 1 cut(s) 397
LweI GCATC 3 cut(s) 52, 772, 1495
MaeI CTAG 8 cut(s) 530, 563, 578, 659, 816, 983, 996, 1265
MaeII ACGT 4 cut(s) 12, 76, 413, 706
MaeIII GTNAC 9 cut(s) 332, 392, 707, 1058, 1191, 1243, 1300, 1331, 1439
MalI GATC 4 cut(s) 111, 348, 703, 1325
MbiI CCGCTC 1 cut(s) 1561
MboI GATC 4 cut(s) 109, 346, 701, 1323
MfeI CAATTG 2 cut(s) 758, 1002
MflI RGATCY 1 cut(s) 1323
MhlI GDGCHC 2 cut(s) 364, 888
MluI ACGCGT 1 cut(s) 1588
MlyI GAGTC 3 cut(s) 882, 1304, 1365
MmeI TCCRAC 4 cut(s) 193, 778, 786, 1264
Mph1103I ATGCAT 1 cut(s) 1510
MroI TCCGGA 1 cut(s) 639
MseI TTAA 3 cut(s) 327, 590, 1565
MslI CAYNNNNRTG 3 cut(s) 357, 465, 1589
MspI CCGG 2 cut(s) 640, 1040
MspR9I CCNGG 2 cut(s) 689, 904
MunI CAATTG 2 cut(s) 758, 1002
Mva1269I GAATGC 2 cut(s) 55, 201
MvaI CCWGG 2 cut(s) 689, 904
MvnI CGCG 1 cut(s) 1590
MwoI GCNNNNNNNGC 6 cut(s) 32, 385, 578, 1145, 1175, 1567
NcoI CCATGG 1 cut(s) 1026
NdeII GATC 4 cut(s) 109, 346, 701, 1323
NheI GCTAGC 3 cut(s) 577, 815, 1264
NlaIV GGNNCC 2 cut(s) 207, 1639
NmeAIII GCCGAG 1 cut(s) 1406
NmuCI GTSAC 2 cut(s) 1243, 1331
NsiI ATGCAT 1 cut(s) 1510
NspI RCATGY 4 cut(s) 167, 623, 679, 1421
NspV TTCGAA 1 cut(s) 605
OliI CACNNNNGTG 2 cut(s) 357, 1589
PaeI GCATGC 1 cut(s) 623
PciI ACATGT 3 cut(s) 163, 675, 1417
PciSI GCTCTTC 1 cut(s) 369
PctI GAATGC 2 cut(s) 55, 201
PfeI GAWTC 2 cut(s) 628, 1579
PflMI CCANNNNNTGG 1 cut(s) 102
PkrI GCNGC 2 cut(s) 387, 1560
PleI GAGTC 3 cut(s) 881, 1304, 1365
PpsI GAGTC 3 cut(s) 881, 1304, 1365
Ppu21I YACGTR 1 cut(s) 707
PscI ACATGT 3 cut(s) 163, 675, 1417
PsiI TTATAA 1 cut(s) 126
Psp6I CCWGG 2 cut(s) 687, 902
PspFI CCCAGC 1 cut(s) 95
PspGI CCWGG 2 cut(s) 687, 902
PspN4I GGNNCC 2 cut(s) 207, 1639
PstNI CAGNNNCTG 1 cut(s) 118
PsuI RGATCY 1 cut(s) 1323
RsaI GTAC 1 cut(s) 513
RsaNI GTAC 1 cut(s) 512
RseI CAYNNNNRTG 3 cut(s) 357, 465, 1589
SalI GTCGAC 1 cut(s) 72
SapI GCTCTTC 1 cut(s) 369
SaqAI TTAA 3 cut(s) 327, 590, 1565
SatI GCNGC 2 cut(s) 386, 1559
Sau3AI GATC 4 cut(s) 109, 346, 701, 1323
SchI GAGTC 3 cut(s) 882, 1304, 1365
ScrFI CCNGG 2 cut(s) 689, 904
SduI GDGCHC 2 cut(s) 364, 888
SfaNI GCATC 3 cut(s) 52, 772, 1495
SfuI TTCGAA 1 cut(s) 605
SmiMI CAYNNNNRTG 3 cut(s) 357, 465, 1589
SmlI CTYRAG 1 cut(s) 30
SmoI CTYRAG 1 cut(s) 30
SphI GCATGC 1 cut(s) 623
SsiI CCGC 4 cut(s) 209, 599, 1023, 1559
SspMI CTAG 8 cut(s) 530, 563, 578, 659, 816, 983, 996, 1265
StyD4I CCNGG 2 cut(s) 687, 902
StyI CCWWGG 1 cut(s) 1026
TaaI ACNGT 5 cut(s) 338, 434, 587, 1071, 1457
TaiI ACGT 4 cut(s) 15, 79, 416, 709
TaqI TCGA 5 cut(s) 73, 605, 945, 1131, 1674
TauI GCSGC 1 cut(s) 1561
TfiI GAWTC 2 cut(s) 628, 1579
Tru1I TTAA 3 cut(s) 327, 590, 1565
Tru9I TTAA 3 cut(s) 327, 590, 1565
TscAI CASTG 5 cut(s) 25, 439, 579, 1074, 1336
TseFI GTSAC 2 cut(s) 1243, 1331
TseI GCWGC 1 cut(s) 385
Tsp45I GTSAC 2 cut(s) 1243, 1331
TspDTI ATGAA 9 cut(s) 393, 507, 1047, 1064, 1166, 1236, 1344, 1460, 1539
TspGWI ACGGA 1 cut(s) 714
TspRI CASTG 5 cut(s) 25, 439, 579, 1074, 1336
Van91I CCANNNNNTGG 1 cut(s) 102
XapI RAATTY 3 cut(s) 312, 714, 1484
XbaI TCTAGA 1 cut(s) 529
XceI RCATGY 4 cut(s) 167, 623, 679, 1421
XcmI CCANNNNNNNNNTGG 1 cut(s) 326
XmiI GTMKAC 3 cut(s) 73, 876, 1605
XspI CTAG 8 cut(s) 530, 563, 578, 659, 816, 983, 996, 1265
Zsp2I ATGCAT 1 cut(s) 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.