Rorug05G0431700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0018407
Physical Location & Seq
Reverse (-)
59985688 .. 59988565
2878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0431700.1

Sequence Viewer

Length: 1611 bp
ATGGAAACCCTGACGGAGCAAACCGACGTCCACGTCATCCTCTCCGAGCAGCGCAGGGAGCTCTTGGCCGCCAAAACTCTAGACGCCGACCTCGACTTGGCCTTCAATCTACAAATGCAAGAAGCCATAACCGCCTCTCTGGCGCTCAATCCATCACCGAGTACTTCACAGTCTCCTCCTCCGTCGCAGCCGTACGAGCCAAACGACGACGTGTTGGACATCGTCGCCACGCTGCTGCTGGAGGACGTGGAGAGGTTCTCGCAGGAGTACGAGGACCACGAGCGCAGCGTTTTGGAGGCGAGGAGGGCGAAGGAGGATCTGGACCGTCGGATTCACGACCAGAAGTTCGCGATCGACGTCCGTGACTTGCCGGAGGAATACTGGAGGAATCACGGGGACGTGTACGAACGGCCGTTCTGTACGGACGGGGCGTCGTCGTCGTCGGGAAAAGCTGCGGCGGAGACAGAGTGTTTGAGATTGTACTGCAAGGGTTTGATTAGTGAAGAGGTTGTGAAGAATGTGAAAATGGCGGTGGGCGGGATTGGGGTTGCGATCTGTGACCCTAGAGATAATCTGGTTTTCGAGGTGAAGAAGAATATGGAGGCGGTGGTTGATGGTGAGAGACTGAGCGATGAGGCTGCGGAGCTTGAGGCTCTGGTTGAAGGGCTTAATAAAGCTCTAACTATGGACTTGAAGAATGTCACATTTTTCTGTGATGATAACAAGCTTTACCAATATTTTACAAACAGAGTGGTTCCTGGAAACAGCAAGATTGCAACACTAGTCAACCAAGTTGCTCTTCTACAAAAAGAATTTGAATCCTGCAGCCCTTCACTTGTGGCACGTGGTGATATTAAGTTTGCATTTAAATTTGCAAGAGAGGCTATAGTTTCTCAGATCACCTGGCAAGCAGAAACTAGCAATGGCAAGTCTTTGAAGGAGACTTGTGTCATATGTTATGAAGAAACTGATGTTGCTCGAATGTTTTCAATCGATGGTTGTCTGCACAGGTATTGCATTTCTTGTATGAAACAGCACGTGGAAGTAAGGTTGCTTAATGGGATGGTGGCACATTGTCCTCATGAAGGCTGCAAATCCGAAGTGAATATTGATAGCTATGAGAATTTCTTCTTGGCACCTAAACTAGTTGAGGTTATGAACCAACGAATCAAGGAGTCTTCTATTCATGTTACAGACAGAGTCTATTGCCAATATCCCAAGTGTTCTGCATTAATGTCCAAGAAGGAGATCTTAGAATATACCAAAACTTCTTATGGTGGTGCTGAAGAATCTGGAGCCAGAAAATGCATGAAATGCCACTACTATTTTTGTATCAAGTGCTTGGTTCCTTGGCATTTTGAATTGACCTGTGATGAGTACCAAACGTCACACAATTTCCCTAAAGAACATCAAATGCTGAAGTCACTGGCTACAAAGAGACTCTGGCGGCAGTGTGTTAAGTGCAACCACATGGTTGAACTTGCACAAGGTTGTTACCACATTACTTGCAGATGTGGATATCAGTTTTGCTACACTTGTGGAGCTGAATGGAAGGACAGGAAACCTTGTTCCTGCCCACTCTGGGACGAGCAGTACATACTTACAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
MPP6

Protein Analysis

536

Amino Acids

61.15

Weight (kDa)

5.36

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 175 - 295 5.5e-20 Reverse transcriptase-like
IBR PF01485 396 - 457 4e-09 IBR domain, a half RING-finger domain
IBR PF01485 473 - 523 7e-07 IBR domain, a half RING-finger domain
Rcat_RNF216 PF26200 474 - 518 6.8e-06 RNF216 Rcat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018407)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g41460
rosa_laevigata RLG00000036475
rosa_multiflora Rmu_sc0000573.1_g000002 Rmu_sc0025084.1_g000001
rosa_roxburghii Rroxscaffold_1G00007100
rosa_rugosa Rorug05G0431700
rosa_samantha Rh5BG509700 Rh5CG533500 Rh5DG522600
rosa_wichuraiana Rw5G045330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 32
AatII GACGTC 2 cut(s) 30, 360
AccB1I GGYRCC 1 cut(s) 1135
AccII CGCG 1 cut(s) 350
AciI CCGC 9 cut(s) 69, 132, 455, 458, 530, 537, 605, 641, 1447
AclWI GGATC 1 cut(s) 324
AcoI YGGCCR 2 cut(s) 66, 410
AcsI RAATTY 3 cut(s) 812, 869, 1123
AcuI CTGAAG 2 cut(s) 1305, 1439
AcvI CACGTG 2 cut(s) 845, 1039
AcyI GRCGYC 4 cut(s) 27, 84, 357, 431
AdeI CACNNNGTG 1 cut(s) 848
AfaI GTAC 8 cut(s) 163, 194, 269, 404, 421, 482, 1379, 1595
AfiI CCNNNNNNNGG 3 cut(s) 97, 1085, 1582
AflIII ACRYGT 2 cut(s) 210, 399
AgsI TTSAA 8 cut(s) 106, 662, 694, 818, 937, 990, 1361, 1478
AhdI GACNNNNNGTC 1 cut(s) 430
AhlI ACTAGT 2 cut(s) 781, 1144
AjiI CACGTC 4 cut(s) 34, 211, 247, 400
AjnI CCWGG 2 cut(s) 757, 902
AjuI GAANNNNNNNTTGG 4 cut(s) 783, 815, 1115, 1147
AloI GAACNNNNNNTCC 2 cut(s) 1552, 1584
AluBI AGCT 7 cut(s) 61, 452, 646, 677, 727, 1116, 1544
AluI AGCT 7 cut(s) 61, 452, 646, 677, 727, 1116, 1544
Alw21I GWGCWC 1 cut(s) 63
Alw26I GTCTC 5 cut(s) 177, 455, 616, 935, 1432
AlwI GGATC 1 cut(s) 324
AoxI GGCC 3 cut(s) 66, 99, 410
ApeKI GCWGC 9 cut(s) 49, 187, 232, 235, 285, 452, 638, 825, 1089
ApoI RAATTY 3 cut(s) 812, 869, 1123
ArsI GACNNNNNNTTYG 4 cut(s) 329, 361, 454, 486
AseI ATTAAT 1 cut(s) 1232
Asp700I GAANNNNTTC 2 cut(s) 985, 1127
AspLEI GCGC 3 cut(s) 54, 145, 285
AspS9I GGNCC 2 cut(s) 274, 322
AsuHPI GGTGA 5 cut(s) 147, 598, 629, 860, 892
AvaII GGWCC 2 cut(s) 274, 322
BanI GGYRCC 1 cut(s) 1135
BanII GRGCYC 1 cut(s) 63
BauI CACGAG 1 cut(s) 278
BbrPI CACGTG 2 cut(s) 845, 1039
BbsI GAAGAC 1 cut(s) 1170
Bbv12I GWGCWC 1 cut(s) 63
BbvI GCAGC 9 cut(s) 61, 199, 219, 222, 297, 439, 625, 837, 1076
BccI CCATC 4 cut(s) 160, 608, 989, 1057
BceAI ACGGC 3 cut(s) 175, 397, 425
BcgI CGANNNNNNTGC 2 cut(s) 620, 654
BciT130I CCWGG 2 cut(s) 759, 904
BcoDI GTCTC 5 cut(s) 177, 455, 616, 935, 1432
BcuI ACTAGT 2 cut(s) 781, 1144
BfaI CTAG 5 cut(s) 80, 564, 782, 918, 1145
BfmI CTRYAG 2 cut(s) 823, 885
BfoI RGCGCY 1 cut(s) 146
BglI GCCNNNNNGGC 1 cut(s) 140
BglII AGATCT 1 cut(s) 1248
BmcAI AGTACT 1 cut(s) 163
Bme1390I CCNGG 2 cut(s) 759, 904
Bme18I GGWCC 2 cut(s) 274, 322
BmeRI GACNNNNNGTC 1 cut(s) 430
BmgBI CACGTC 4 cut(s) 34, 211, 247, 400
BmgT120I GGNCC 2 cut(s) 274, 322
BmiI GGNNCC 4 cut(s) 756, 1137, 1297, 1347
BmrFI CCNGG 2 cut(s) 759, 904
BoxI GACNNNNGTC 1 cut(s) 947
BpiI GAAGAC 1 cut(s) 1170
BplI GAGNNNNNCTC 2 cut(s) 242, 274
BpmI CTGGAG 3 cut(s) 260, 403, 1314
BpuEI CTTGAG 1 cut(s) 668
Bsa29I ATCGAT 1 cut(s) 993
BsaAI YACGTR 2 cut(s) 845, 1039
BsaHI GRCGYC 4 cut(s) 27, 84, 357, 431
BsaJI CCNNGG 1 cut(s) 1349
BsaXI ACNNNNNCTCC 2 cut(s) 452, 482
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 1085, 1582
Bse1I ACTGG 2 cut(s) 386, 1431
Bse3DI GCAATG 1 cut(s) 928
BseBI CCWGG 2 cut(s) 759, 904
BseCI ATCGAT 1 cut(s) 993
BseDI CCNNGG 1 cut(s) 1349
BseGI GGATG 2 cut(s) 36, 1068
BseLI CCNNNNNNNGG 3 cut(s) 97, 1085, 1582
BseMI GCAATG 1 cut(s) 928
BseMII CTCAG 2 cut(s) 617, 908
BseNI ACTGG 2 cut(s) 386, 1431
BseRI GAGGAG 3 cut(s) 165, 168, 316
BseX3I CGGCCG 1 cut(s) 410
BseXI GCAGC 9 cut(s) 61, 199, 219, 222, 297, 439, 625, 837, 1076
BsgI GTGCAG 1 cut(s) 989
Bsh1236I CGCG 1 cut(s) 350
Bsh1285I CGRYCG 2 cut(s) 354, 413
BshFI GGCC 3 cut(s) 68, 101, 412
BshNI GGYRCC 1 cut(s) 1135
BshVI ATCGAT 1 cut(s) 993
BsiEI CGRYCG 2 cut(s) 354, 413
BsiHKAI GWGCWC 1 cut(s) 63
BsiSI CCGG 1 cut(s) 371
BsiWI CGTACG 1 cut(s) 192
BslFI GGGAC 2 cut(s) 410, 1598
BslI CCNNNNNNNGG 3 cut(s) 97, 1085, 1582
BsmAI GTCTC 5 cut(s) 177, 455, 616, 935, 1432
BsmFI GGGAC 2 cut(s) 410, 1598
BsnI GGCC 3 cut(s) 68, 101, 412
Bsp1286I GDGCHC 1 cut(s) 63
Bsp143I GATC 5 cut(s) 316, 351, 552, 897, 1248
Bsp68I TCGCGA 1 cut(s) 350
BspACI CCGC 9 cut(s) 69, 132, 455, 458, 530, 537, 605, 641, 1447
BspANI GGCC 3 cut(s) 68, 101, 412
BspCNI CTCAG 2 cut(s) 618, 907
BspDI ATCGAT 1 cut(s) 993
BspFNI CGCG 1 cut(s) 350
BspHI TCATGA 1 cut(s) 1081
BspLI GGNNCC 4 cut(s) 756, 1137, 1297, 1347
BspMAI CTGCAG 1 cut(s) 827
BspPI GGATC 1 cut(s) 324
BspQI GCTCTTC 1 cut(s) 804
BspT107I GGYRCC 1 cut(s) 1135
BsrDI GCAATG 1 cut(s) 928
BsrI ACTGG 2 cut(s) 386, 1431
BssECI CCNNGG 1 cut(s) 1349
BssMI GATC 5 cut(s) 316, 351, 552, 897, 1248
BssNI GRCGYC 4 cut(s) 27, 84, 357, 431
BssSI CACGAG 1 cut(s) 278
BssT1I CCWWGG 1 cut(s) 1349
Bst2BI CACGAG 1 cut(s) 278
Bst2UI CCWGG 2 cut(s) 759, 904
Bst4CI ACNGT 2 cut(s) 171, 326
Bst6I CTCTTC 2 cut(s) 498, 804
BstACI GRCGYC 4 cut(s) 27, 84, 357, 431
BstAPI GCANNNNNTGC 1 cut(s) 1314
BstBAI YACGTR 2 cut(s) 845, 1039
BstC8I GCNNGC 1 cut(s) 909
BstDEI CTNAG 3 cut(s) 626, 894, 1252
BstENI CCTNNNNNAGG 1 cut(s) 1083
BstF5I GGATG 2 cut(s) 36, 1068
BstFNI CGCG 1 cut(s) 350
BstH2I RGCGCY 1 cut(s) 146
BstHHI GCGC 3 cut(s) 54, 145, 285
BstKTI GATC 5 cut(s) 319, 354, 555, 900, 1251
BstMAI GTCTC 5 cut(s) 177, 455, 616, 935, 1432
BstMBI GATC 5 cut(s) 316, 351, 552, 897, 1248
BstMCI CGRYCG 2 cut(s) 354, 413
BstMWI GCNNNNNNNGC 7 cut(s) 58, 131, 140, 196, 305, 881, 1314
BstNI CCWGG 2 cut(s) 759, 904
BstPAI GACNNNNGTC 1 cut(s) 947
BstSCI CCNGG 2 cut(s) 757, 902
BstSFI CTRYAG 2 cut(s) 823, 885
BstUI CGCG 1 cut(s) 350
BstV1I GCAGC 9 cut(s) 61, 199, 219, 222, 297, 439, 625, 837, 1076
BstV2I GAAGAC 1 cut(s) 1170
BstX2I RGATCY 2 cut(s) 316, 1248
BstYI RGATCY 2 cut(s) 316, 1248
BstZI CGGCCG 1 cut(s) 410
Bsu15I ATCGAT 1 cut(s) 993
BsuRI GGCC 3 cut(s) 68, 101, 412
BsuTUI ATCGAT 1 cut(s) 993
BtgZI GCGATG 1 cut(s) 645
BtrI CACGTC 4 cut(s) 34, 211, 247, 400
BtsCI GGATG 2 cut(s) 36, 1068
BtsI GCAGTG 1 cut(s) 1457
BtsIMutI CAGTG 2 cut(s) 1424, 1457
BtuMI TCGCGA 1 cut(s) 350
Cac8I GCNNGC 1 cut(s) 909
CciI TCATGA 1 cut(s) 1081
CfoI GCGC 3 cut(s) 54, 145, 285
Cfr13I GGNCC 2 cut(s) 274, 322
ClaI ATCGAT 1 cut(s) 993
CseI GACGC 2 cut(s) 92, 420
Csp6I GTAC 8 cut(s) 162, 193, 268, 403, 420, 481, 1378, 1594
CspCI CAANNNNNGTGG 4 cut(s) 732, 767, 1487, 1522
CviAII CATG 4 cut(s) 1082, 1187, 1309, 1471
CviQI GTAC 8 cut(s) 162, 193, 268, 403, 420, 481, 1378, 1594
DdeI CTNAG 3 cut(s) 626, 894, 1252
DpnI GATC 5 cut(s) 318, 353, 554, 899, 1250
DpnII GATC 5 cut(s) 316, 351, 552, 897, 1248
DraI TTTAAA 1 cut(s) 868
DraIII CACNNNGTG 1 cut(s) 848
DrdI GACNNNNNNGTC 1 cut(s) 32
DriI GACNNNNNGTC 1 cut(s) 430
DseDI GACNNNNNNGTC 1 cut(s) 32
EaeI YGGCCR 2 cut(s) 66, 410
EagI CGGCCG 1 cut(s) 410
Eam1104I CTCTTC 2 cut(s) 498, 804
Eam1105I GACNNNNNGTC 1 cut(s) 430
EarI CTCTTC 2 cut(s) 498, 804
EciI GGCGGA 1 cut(s) 473
Ecl136II GAGCTC 1 cut(s) 61
EclXI CGGCCG 1 cut(s) 410
Eco130I CCWWGG 1 cut(s) 1349
Eco24I GRGCYC 1 cut(s) 63
Eco32I GATATC 1 cut(s) 1520
Eco47I GGWCC 2 cut(s) 274, 322
Eco52I CGGCCG 1 cut(s) 410
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 2 cut(s) 1305, 1439
Eco72I CACGTG 2 cut(s) 845, 1039
EcoICRI GAGCTC 1 cut(s) 61
EcoNI CCTNNNNNAGG 1 cut(s) 1083
EcoRII CCWGG 2 cut(s) 757, 902
EcoRV GATATC 1 cut(s) 1520
EcoT14I CCWWGG 1 cut(s) 1349
EcoT22I ATGCAT 1 cut(s) 1310
EcoT38I GRGCYC 1 cut(s) 63
ErhI CCWWGG 1 cut(s) 1349
FaeI CATG 4 cut(s) 1085, 1190, 1312, 1474
FalI AAGNNNNNCTT 4 cut(s) 783, 815, 1235, 1267
FaqI GGGAC 2 cut(s) 410, 1598
FatI CATG 4 cut(s) 1081, 1186, 1308, 1470
FauI CCCGC 1 cut(s) 530
FauNDI CATATG 1 cut(s) 953
FokI GGATG 2 cut(s) 23, 1075
FriOI GRGCYC 1 cut(s) 63
FspBI CTAG 5 cut(s) 80, 564, 782, 918, 1145
GlaI GCGC 3 cut(s) 53, 144, 284
GsuI CTGGAG 3 cut(s) 260, 403, 1314
HaeII RGCGCY 1 cut(s) 146
HaeIII GGCC 3 cut(s) 68, 101, 412
HapII CCGG 1 cut(s) 371
HgaI GACGC 2 cut(s) 92, 420
HhaI GCGC 3 cut(s) 54, 145, 285
Hin1I GRCGYC 4 cut(s) 27, 84, 357, 431
Hin1II CATG 4 cut(s) 1085, 1190, 1312, 1474
Hin6I GCGC 3 cut(s) 52, 143, 283
HinP1I GCGC 3 cut(s) 52, 143, 283
HincII GTYRAC 1 cut(s) 787
HindII GTYRAC 1 cut(s) 787
HindIII AAGCTT 1 cut(s) 725
HinfI GANTC 8 cut(s) 331, 388, 818, 1167, 1175, 1200, 1289, 1440
HpaII CCGG 1 cut(s) 371
HphI GGTGA 5 cut(s) 147, 598, 629, 860, 892
Hpy166II GTNNAC 3 cut(s) 31, 403, 787
Hpy188I TCNGA 4 cut(s) 46, 330, 897, 1099
Hpy188III TCNNGA 7 cut(s) 80, 320, 335, 349, 444, 1082, 1293
Hpy8I GTNNAC 3 cut(s) 31, 403, 787
HpyAV CCTTC 8 cut(s) 112, 304, 656, 840, 931, 1079, 1237, 1546
HpyCH4III ACNGT 2 cut(s) 171, 326
HpyCH4IV ACGT 9 cut(s) 27, 33, 210, 246, 357, 399, 844, 1038, 1385
HpyF10VI GCNNNNNNNGC 7 cut(s) 58, 131, 140, 196, 305, 881, 1314
HpyF3I CTNAG 3 cut(s) 626, 894, 1252
HpySE526I ACGT 9 cut(s) 27, 33, 210, 246, 357, 399, 844, 1038, 1385
Hsp92I GRCGYC 4 cut(s) 27, 84, 357, 431
Hsp92II CATG 4 cut(s) 1085, 1190, 1312, 1474
HspAI GCGC 3 cut(s) 52, 143, 283
Kzo9I GATC 5 cut(s) 316, 351, 552, 897, 1248
LguI GCTCTTC 1 cut(s) 804
LmnI GCTCC 5 cut(s) 16, 58, 643, 1295, 1541
Lsp1109I GCAGC 9 cut(s) 61, 199, 219, 222, 297, 439, 625, 837, 1076
MaeI CTAG 5 cut(s) 80, 564, 782, 918, 1145
MaeII ACGT 9 cut(s) 27, 33, 210, 246, 357, 399, 844, 1038, 1385
MaeIII GTNAC 7 cut(s) 362, 557, 700, 1189, 1386, 1422, 1493
MalI GATC 5 cut(s) 318, 353, 554, 899, 1250
MboI GATC 5 cut(s) 316, 351, 552, 897, 1248
MflI RGATCY 2 cut(s) 316, 1248
MhlI GDGCHC 1 cut(s) 63
MluCI AATT 5 cut(s) 812, 869, 1123, 1361, 1393
MlyI GAGTC 3 cut(s) 1184, 1209, 1434
MmeI TCCRAC 2 cut(s) 195, 308
Mph1103I ATGCAT 1 cut(s) 1310
MroXI GAANNNNTTC 2 cut(s) 985, 1127
MseI TTAA 6 cut(s) 669, 855, 867, 1056, 1232, 1458
MspI CCGG 1 cut(s) 371
MspR9I CCNGG 2 cut(s) 759, 904
MvaI CCWGG 2 cut(s) 759, 904
MvnI CGCG 1 cut(s) 350
MwoI GCNNNNNNNGC 7 cut(s) 58, 131, 140, 196, 305, 881, 1314
NdeI CATATG 1 cut(s) 953
NdeII GATC 5 cut(s) 316, 351, 552, 897, 1248
NlaIII CATG 4 cut(s) 1085, 1190, 1312, 1474
NlaIV GGNNCC 4 cut(s) 756, 1137, 1297, 1347
NmuCI GTSAC 5 cut(s) 362, 557, 700, 1386, 1422
NruI TCGCGA 1 cut(s) 350
NsiI ATGCAT 1 cut(s) 1310
PagI TCATGA 1 cut(s) 1081
PciSI GCTCTTC 1 cut(s) 804
PcsI WCGNNNNNNNCGW 6 cut(s) 90, 201, 276, 285, 354, 428
PdmI GAANNNNTTC 2 cut(s) 985, 1127
PfeI GAWTC 5 cut(s) 331, 388, 818, 1167, 1289
Pfl23II CGTACG 1 cut(s) 192
PflFI GACNNNGTC 2 cut(s) 221, 1199
PfoI TCCNGGA 1 cut(s) 757
Ple19I CGATCG 1 cut(s) 354
PleI GAGTC 3 cut(s) 1183, 1208, 1434
PmaCI CACGTG 2 cut(s) 845, 1039
PmlI CACGTG 2 cut(s) 845, 1039
PpsI GAGTC 3 cut(s) 1183, 1208, 1434
Ppu21I YACGTR 2 cut(s) 845, 1039
PshAI GACNNNNGTC 1 cut(s) 947
PshBI ATTAAT 1 cut(s) 1232
Psp124BI GAGCTC 1 cut(s) 63
Psp6I CCWGG 2 cut(s) 757, 902
PspCI CACGTG 2 cut(s) 845, 1039
PspGI CCWGG 2 cut(s) 757, 902
PspLI CGTACG 1 cut(s) 192
PspN4I GGNNCC 4 cut(s) 756, 1137, 1297, 1347
PspPI GGNCC 2 cut(s) 274, 322
PstI CTGCAG 1 cut(s) 827
PsuI RGATCY 2 cut(s) 316, 1248
PsyI GACNNNGTC 2 cut(s) 221, 1199
PvuI CGATCG 1 cut(s) 354
RruI TCGCGA 1 cut(s) 350
RsaI GTAC 8 cut(s) 163, 194, 269, 404, 421, 482, 1379, 1595
RsaNI GTAC 8 cut(s) 162, 193, 268, 403, 420, 481, 1378, 1594
SacI GAGCTC 1 cut(s) 63
SapI GCTCTTC 1 cut(s) 804
SaqAI TTAA 6 cut(s) 669, 855, 867, 1056, 1232, 1458
Sau3AI GATC 5 cut(s) 316, 351, 552, 897, 1248
Sau96I GGNCC 2 cut(s) 274, 322
ScaI AGTACT 1 cut(s) 163
SchI GAGTC 3 cut(s) 1184, 1209, 1434
ScrFI CCNGG 2 cut(s) 759, 904
SduI GDGCHC 1 cut(s) 63
SfcI CTRYAG 2 cut(s) 823, 885
SinI GGWCC 2 cut(s) 274, 322
SmiI ATTTAAAT 1 cut(s) 868
SmlI CTYRAG 1 cut(s) 647
SmoI CTYRAG 1 cut(s) 647
SpeI ACTAGT 2 cut(s) 781, 1144
Sse9I AATT 5 cut(s) 812, 869, 1123, 1361, 1393
SsiI CCGC 9 cut(s) 69, 132, 455, 458, 530, 537, 605, 641, 1447
SspI AATATT 2 cut(s) 737, 1108
SspMI CTAG 5 cut(s) 80, 564, 782, 918, 1145
SstI GAGCTC 1 cut(s) 63
StyD4I CCNGG 2 cut(s) 757, 902
StyI CCWWGG 1 cut(s) 1349
SwaI ATTTAAAT 1 cut(s) 868
TaaI ACNGT 2 cut(s) 171, 326
TaiI ACGT 9 cut(s) 30, 36, 213, 249, 360, 402, 847, 1041, 1388
TaqI TCGA 5 cut(s) 93, 354, 582, 979, 993
TasI AATT 5 cut(s) 812, 869, 1123, 1361, 1393
TatI WGTACW 3 cut(s) 161, 480, 1593
TauI GCSGC 3 cut(s) 71, 458, 1450
TfiI GAWTC 5 cut(s) 331, 388, 818, 1167, 1289
Tru1I TTAA 6 cut(s) 669, 855, 867, 1056, 1232, 1458
Tru9I TTAA 6 cut(s) 669, 855, 867, 1056, 1232, 1458
TscAI CASTG 2 cut(s) 1431, 1457
TseFI GTSAC 5 cut(s) 362, 557, 700, 1386, 1422
TseI GCWGC 9 cut(s) 49, 187, 232, 235, 285, 452, 638, 825, 1089
Tsp45I GTSAC 5 cut(s) 362, 557, 700, 1386, 1422
TspDTI ATGAA 6 cut(s) 975, 1043, 1098, 1172, 1175, 1325
TspGWI ACGGA 4 cut(s) 29, 171, 350, 437
TspRI CASTG 2 cut(s) 1431, 1457
Tth111I GACNNNGTC 2 cut(s) 221, 1199
VpaK11BI GGWCC 2 cut(s) 274, 322
VspI ATTAAT 1 cut(s) 1232
XagI CCTNNNNNAGG 1 cut(s) 1083
XapI RAATTY 3 cut(s) 812, 869, 1123
XbaI TCTAGA 1 cut(s) 79
XcmI CCANNNNNNNNNTGG 1 cut(s) 235
XmnI GAANNNNTTC 2 cut(s) 985, 1127
XspI CTAG 5 cut(s) 80, 564, 782, 918, 1145
ZraI GACGTC 2 cut(s) 28, 358
ZrmI AGTACT 1 cut(s) 163
Zsp2I ATGCAT 1 cut(s) 1310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.