Rorug06G0294800

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0016632
Physical Location & Seq
Forward (+)
44695067 .. 44695982
916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0294800.1

Sequence Viewer

Length: 666 bp
ATGAGTTGGAGCCACCCAGATATTTCATTGGAAGAATTCATGAAACTGGTTAAAGGGTTCGTCGATATTCTGATTCTGCTTTCTGGGTACCAATCCTCTGGTCGTCTTGCTCACTGGGACTCCCAGAATATCAAGAAGGCCTTCCAATGGGGCCTCTTCTTCGAAAATTTTCTGGGGAGCTTTAGCAGCTCTGATGATTATGTGGACTCTGTGATGGAACTTGATGGGGCTATTTCTGACATGACATCTGCTGCTTCTTTCCCTAAGGGCCTTGCACATATGTCAACTGCTACTCTTGCTACCGCGCGGCGTTTTGTGTTAGAGCATCTCATCCATAGTTTACCTTTGAGAGATGCGCATCTGAGAGCTTTGCTGACGGCGACTGTGGAGATGGATCTTGAGGAGCTATCGGAAAGTGAGCATGATTTTCTTAATGTGTATCTCAACAAGTTGAAGCTGGAGAATACACCAATTCAGCAAGGCATTGAGTCGGCTAGAAAGTTTGGAGATTGCGGTGGTAATGGTTTGACGAAATATGCGGTTCAAGGGCTCTTGAAGAGGTGGTCTGTGGTTTCTTTGATATCAACGGTTGAGTCAGGCTTAGACGTTCTTTCTCGAAGTGTCAGACACAGCACAGTTGGAGTGAGTTCGATGATAACTTATTGA

Protein Analysis

221

Amino Acids

24.52

Weight (kDa)

5.57

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FANCF PF11107 11 - 135 1.7e-09 Fanconi anemia group F protein (FANCF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0016632)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47530 AT1G47530
fragaria_vesca FvH4_2g40430
malus_domestica MD00G1039400.v1.1 MD08G1037600.v1.1
prunus_persica Prupe.1G384800_v2.0.a1
pyrus_communis pycom08g02990
rosa_chinensis RchiOBHm_Chr6g0300301
rosa_laevigata RLG00000011349
rosa_multiflora Rmu_sc0001670.1_g000023
rosa_roxburghii Rroxscaffold_7G00167910
rosa_rugosa Rorug06G0294800
rosa_samantha Rh6CG422300
rosa_wichuraiana Rw6G035630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 357
Acc65I GGTACC 1 cut(s) 87
AccB1I GGYRCC 1 cut(s) 87
AccII CGCG 2 cut(s) 305, 307
AciI CCGC 4 cut(s) 303, 307, 513, 539
AclWI GGATC 1 cut(s) 402
AcsI RAATTY 2 cut(s) 35, 166
AfaI GTAC 1 cut(s) 89
AfiI CCNNNNNNNGG 1 cut(s) 147
AgsI TTSAA 3 cut(s) 454, 545, 556
AluBI AGCT 5 cut(s) 180, 189, 368, 406, 457
AluI AGCT 5 cut(s) 180, 189, 368, 406, 457
AlwI GGATC 1 cut(s) 402
AoxI GGCC 3 cut(s) 138, 151, 268
ApeKI GCWGC 2 cut(s) 186, 251
ApoI RAATTY 2 cut(s) 35, 166
Asp700I GAANNNNTTC 2 cut(s) 140, 168
Asp718I GGTACC 1 cut(s) 87
AspLEI GCGC 2 cut(s) 307, 358
AspS9I GGNCC 2 cut(s) 151, 268
AsuII TTCGAA 1 cut(s) 162
AxyI CCTNAGG 1 cut(s) 264
BanI GGYRCC 1 cut(s) 87
BanII GRGCYC 1 cut(s) 552
BbvI GCAGC 2 cut(s) 198, 238
BccI CCATC 3 cut(s) 208, 218, 385
BceAI ACGGC 1 cut(s) 393
BfaI CTAG 1 cut(s) 495
BisI GCNGC 3 cut(s) 187, 252, 308
BlsI GCNGC 3 cut(s) 188, 253, 309
BmgT120I GGNCC 2 cut(s) 151, 268
BmiI GGNNCC 3 cut(s) 11, 89, 152
BmrI ACTGGG 1 cut(s) 124
BmsI GCATC 3 cut(s) 334, 343, 367
BmuI ACTGGG 1 cut(s) 124
BpmI CTGGAG 1 cut(s) 479
Bpu14I TTCGAA 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 419
BsaBI GATNNNNATC 1 cut(s) 357
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse1I ACTGG 2 cut(s) 51, 119
Bse21I CCTNAGG 1 cut(s) 264
Bse8I GATNNNNATC 1 cut(s) 357
BseGI GGATG 1 cut(s) 330
BseJI GATNNNNATC 1 cut(s) 357
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 2 cut(s) 51, 119
BseRI GAGGAG 1 cut(s) 416
BseXI GCAGC 2 cut(s) 198, 238
Bsh1236I CGCG 2 cut(s) 305, 307
BshFI GGCC 3 cut(s) 140, 153, 270
BshNI GGYRCC 1 cut(s) 87
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 3 cut(s) 140, 153, 270
Bsp119I TTCGAA 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 552
Bsp143I GATC 1 cut(s) 394
BspACI CCGC 4 cut(s) 303, 307, 513, 539
BspANI GGCC 3 cut(s) 140, 153, 270
BspCNI CTCAG 1 cut(s) 354
BspFNI CGCG 2 cut(s) 305, 307
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 3 cut(s) 11, 89, 152
BspPI GGATC 1 cut(s) 402
BspT104I TTCGAA 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 87
BsrI ACTGG 2 cut(s) 51, 119
BssMI GATC 1 cut(s) 394
Bst4CI ACNGT 3 cut(s) 385, 589, 637
Bst6I CTCTTC 2 cut(s) 161, 551
BstBI TTCGAA 1 cut(s) 162
BstDEI CTNAG 3 cut(s) 264, 362, 601
BstF5I GGATG 1 cut(s) 330
BstFNI CGCG 2 cut(s) 305, 307
BstHHI GCGC 2 cut(s) 307, 358
BstKTI GATC 1 cut(s) 397
BstMBI GATC 1 cut(s) 394
BstMWI GCNNNNNNNGC 2 cut(s) 186, 296
BstUI CGCG 2 cut(s) 305, 307
BstV1I GCAGC 2 cut(s) 198, 238
BstX2I RGATCY 1 cut(s) 394
BstXI CCANNNNNNTGG 1 cut(s) 98
BstYI RGATCY 1 cut(s) 394
Bsu36I CCTNAGG 1 cut(s) 264
BsuRI GGCC 3 cut(s) 140, 153, 270
BtsCI GGATG 1 cut(s) 330
BtsIMutI CAGTG 1 cut(s) 112
CciI TCATGA 1 cut(s) 39
CfoI GCGC 2 cut(s) 307, 358
Cfr13I GGNCC 2 cut(s) 151, 268
Csp6I GTAC 1 cut(s) 88
CviAII CATG 3 cut(s) 40, 241, 422
CviQI GTAC 1 cut(s) 88
DdeI CTNAG 3 cut(s) 264, 362, 601
DpnI GATC 1 cut(s) 396
DpnII GATC 1 cut(s) 394
Eam1104I CTCTTC 2 cut(s) 161, 551
EarI CTCTTC 2 cut(s) 161, 551
Eco147I AGGCCT 1 cut(s) 140
Eco24I GRGCYC 1 cut(s) 552
Eco32I GATATC 1 cut(s) 582
Eco81I CCTNAGG 1 cut(s) 264
EcoO109I RGGNCCY 2 cut(s) 151, 268
EcoRI GAATTC 1 cut(s) 35
EcoRV GATATC 1 cut(s) 582
EcoT38I GRGCYC 1 cut(s) 552
FaeI CATG 3 cut(s) 43, 244, 425
FaiI YATR 8 cut(s) 41, 201, 242, 279, 281, 336, 423, 537
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FaqI GGGAC 1 cut(s) 131
FatI CATG 3 cut(s) 39, 240, 421
FauNDI CATATG 1 cut(s) 279
Fnu4HI GCNGC 3 cut(s) 187, 252, 308
FokI GGATG 1 cut(s) 317
FriOI GRGCYC 1 cut(s) 552
Fsp4HI GCNGC 3 cut(s) 187, 252, 308
FspAI RTGCGCAY 1 cut(s) 357
FspBI CTAG 1 cut(s) 495
FspI TGCGCA 1 cut(s) 357
GlaI GCGC 2 cut(s) 306, 357
GluI GCNGC 3 cut(s) 187, 252, 308
GsuI CTGGAG 1 cut(s) 479
HaeIII GGCC 3 cut(s) 140, 153, 270
HhaI GCGC 2 cut(s) 307, 358
Hin1II CATG 3 cut(s) 43, 244, 425
Hin6I GCGC 2 cut(s) 305, 356
HinP1I GCGC 2 cut(s) 305, 356
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HinfI GANTC 5 cut(s) 73, 119, 206, 488, 593
Hpy166II GTNNAC 3 cut(s) 205, 285, 341
Hpy188I TCNGA 6 cut(s) 72, 193, 238, 363, 412, 626
Hpy188III TCNNGA 5 cut(s) 40, 133, 398, 553, 615
Hpy8I GTNNAC 3 cut(s) 205, 285, 341
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 2 cut(s) 130, 151
HpyCH4III ACNGT 3 cut(s) 385, 589, 637
HpyCH4IV ACGT 1 cut(s) 606
HpyCH4V TGCA 1 cut(s) 275
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 296
HpyF3I CTNAG 3 cut(s) 264, 362, 601
HpySE526I ACGT 1 cut(s) 606
Hsp92II CATG 3 cut(s) 43, 244, 425
HspAI GCGC 2 cut(s) 305, 356
KpnI GGTACC 1 cut(s) 91
Kzo9I GATC 1 cut(s) 394
LmnI GCTCC 3 cut(s) 9, 177, 403
LpnPI CCDG 9 cut(s) 30, 32, 69, 84, 100, 137, 158, 443, 582
Lsp1109I GCAGC 2 cut(s) 198, 238
LweI GCATC 3 cut(s) 334, 343, 367
MaeI CTAG 1 cut(s) 495
MaeII ACGT 1 cut(s) 606
MalI GATC 1 cut(s) 396
MboI GATC 1 cut(s) 394
MboII GAAGA 4 cut(s) 44, 148, 151, 568
MflI RGATCY 1 cut(s) 394
MhlI GDGCHC 1 cut(s) 552
MluCI AATT 3 cut(s) 35, 166, 471
MlyI GAGTC 4 cut(s) 113, 200, 497, 602
MmeI TCCRAC 1 cut(s) 619
MnlI CCTC 4 cut(s) 106, 164, 394, 552
MroXI GAANNNNTTC 2 cut(s) 140, 168
MseI TTAA 2 cut(s) 51, 432
MvnI CGCG 2 cut(s) 305, 307
MwoI GCNNNNNNNGC 2 cut(s) 186, 296
NdeI CATATG 1 cut(s) 279
NdeII GATC 1 cut(s) 394
NlaIII CATG 3 cut(s) 43, 244, 425
NlaIV GGNNCC 3 cut(s) 11, 89, 152
NsbI TGCGCA 1 cut(s) 357
NspV TTCGAA 1 cut(s) 162
PagI TCATGA 1 cut(s) 39
PceI AGGCCT 1 cut(s) 140
PdmI GAANNNNTTC 2 cut(s) 140, 168
PfeI GAWTC 1 cut(s) 73
PkrI GCNGC 3 cut(s) 188, 253, 309
PleI GAGTC 4 cut(s) 113, 200, 496, 601
PpsI GAGTC 4 cut(s) 113, 200, 496, 601
PspN4I GGNNCC 3 cut(s) 11, 89, 152
PspPI GGNCC 2 cut(s) 151, 268
PsuI RGATCY 1 cut(s) 394
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
SaqAI TTAA 2 cut(s) 51, 432
SatI GCNGC 3 cut(s) 187, 252, 308
Sau3AI GATC 1 cut(s) 394
Sau96I GGNCC 2 cut(s) 151, 268
SchI GAGTC 4 cut(s) 113, 200, 497, 602
SduI GDGCHC 1 cut(s) 552
SetI ASST 8 cut(s) 182, 191, 346, 370, 408, 459, 563, 609
SfaNI GCATC 3 cut(s) 334, 343, 367
SfuI TTCGAA 1 cut(s) 162
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 3 cut(s) 35, 166, 471
SseBI AGGCCT 1 cut(s) 140
SsiI CCGC 4 cut(s) 303, 307, 513, 539
SspMI CTAG 1 cut(s) 495
StuI AGGCCT 1 cut(s) 140
TaaI ACNGT 3 cut(s) 385, 589, 637
TaiI ACGT 1 cut(s) 609
TaqI TCGA 4 cut(s) 63, 162, 616, 650
TasI AATT 3 cut(s) 35, 166, 471
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 1 cut(s) 73
Tru1I TTAA 2 cut(s) 51, 432
Tru9I TTAA 2 cut(s) 51, 432
TscAI CASTG 1 cut(s) 119
TseI GCWGC 2 cut(s) 186, 251
TspDTI ATGAA 3 cut(s) 15, 28, 56
TspRI CASTG 1 cut(s) 119
XapI RAATTY 2 cut(s) 35, 166
XmnI GAANNNNTTC 2 cut(s) 140, 168
XspI CTAG 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.